Journal: Metabolites

Article Title: Identification of Plasma Metabolites Responding to Oxycodone Exposure in Rats

doi: 10.3390/metabo15020095

Figure Lengend Snippet: Tryptophan metabolism sub-pathway. Metabolites highlighted in green show a significant increase in abundance level at intoxication compared to pre-exposure. Metabolites in red did not have significant differential responses between the two time points. Metabolites in black were not measurable in our study.

Article Snippet: A total of 941 annotated metabolites were characterized by Metabolon.

Techniques:

Journal: Journal of Translational Medicine

Article Title: Integrative microbiome and metabolome profiles reveal the impacts of periodontitis via oral-gut axis in first-trimester pregnant women

doi: 10.1186/s12967-024-05579-9

Figure Lengend Snippet: Comparison of fecal metabolites between the pregnant women with Perio ( n = 31) and Non-Perio ( n = 23). A Principal component analysis score plot. 95% confidence ellipses were shown. B Volcano plot revealed the inter-group difference in metabolic features ( n = 137). Features with |log 2 FC| > 1 and P < 0.05 (Wilcoxon signed-rank test) were colored and labelled. Chemical classification of metabolic features in samples ( C ) and limma-identified and BMI-adjusted differentially changed ( P < 0.05) metabolic features ( D , n = 547) of feces, saliva and serum based on ClassyFire classification. E Boxplot of selected differentially changed metabolic features in feces. Wilcoxon rank sum test. F Functional enrichment analysis on limma-identified and BMI-adjusted differentially changed ( P < 0.05) metabolic features in feces ( n = 547) with integration of mummichog and GSEA algorithms. Quantitative metabolite set enrichment analysis (qMSEA) on MS2- and HMDB-annotated fecal metabolic features (limma-identified BMI-adjusted differentially changed, P < 0.05) for pathways ( G ) and disease signatures of HMDB ( H ). The detailed results are presented in Tables –

Article Snippet: Of them, 92 were MS2-annotated, including 56 MS2-annotated metabolites associated with Perio (positive coefficients).

Techniques: Comparison, Functional Assay

Journal: Journal of Translational Medicine

Article Title: Integrative microbiome and metabolome profiles reveal the impacts of periodontitis via oral-gut axis in first-trimester pregnant women

doi: 10.1186/s12967-024-05579-9

Figure Lengend Snippet: Integration of fecal microbiota, metabolites and systemic clinical determinants in the pregnant women with Perio ( n = 31) and Non-Perio ( n = 23). A DIABLO-integrated correlation network of fecal microbiome, metabolome and clinical numeric determinants. Nodes of ‘tie’ of sPLS-DA were not shown. The line width represented | r |. The correlation coefficient threshold with clinical determinants was |r|>0.3, while the one between microbiome and metabolome was |r|>0.4. Two clusters were observed, and all the inter-cluster links were shown with translucency for clarity. When no HMDB ID was presented, the feature was annotated by an in-house library only. CRP, C-reactive protein; TG, triglyceride; LDL-c, low-density lipoprotein cholesterol; TBIL, total bilirubin; TP, total protein; ALB, serum albumin. B Pairwise Spearman’s correlation analysis between DESeq2-enriched genera (relative abundance) in feces and Venn-selected MS2-annotated metabolic features with FDR (Benjamini-Hochberg) adjustment for P values. Enriched groups for microbiome were from DESeq2 results, while enriched groups for metabolome were from the sPLS-DA model. Correlation matrices were presented with asterisks representing the significance of FDR-adjusted P values: * FDR < 0.05, ** FDR < 0.01. The exact adjusted P values (FDR) are provided in Table . c Scatter plots of Spearman’s correlation between selected fecal genera and metabolic features

Article Snippet: Of them, 92 were MS2-annotated, including 56 MS2-annotated metabolites associated with Perio (positive coefficients).

Techniques: