mes d3 cells Search Results


d3 mes cells  (ATCC)
93
ATCC d3 mes cells
Gli2 and MEF2C are expressed <t>during</t> <t>mES</t> differentiation. ( A ) Schematic representation of cardiomyogenesis in <t>D3</t> mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.
D3 Mes Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mes+d3+cells/ES-D3+(D3)%3B+Stem+Cell%3B+Mouse/pmc03333882-121-0-3
Average 93 stars, based on 1 article reviews
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mes cell line d3  (ATCC)
96
ATCC mes cell line d3
Gli2 and MEF2C are expressed <t>during</t> <t>mES</t> differentiation. ( A ) Schematic representation of cardiomyogenesis in <t>D3</t> mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.
Mes Cell Line D3, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mes+d3+cells/ES-D3/pm26330232-47-8-13
Average 96 stars, based on 1 article reviews
mes cell line d3 - by Bioz Stars, 2026-08
96/100 stars
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murine cell line d3  (ATCC)
96
ATCC murine cell line d3
Gli2 and MEF2C are expressed <t>during</t> <t>mES</t> differentiation. ( A ) Schematic representation of cardiomyogenesis in <t>D3</t> mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.
Murine Cell Line D3, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mes+d3+cells/MES-SA%2FMX2%3B+Uterine+Sarcoma%3B+Human/us07868133-524-6-10
Average 96 stars, based on 1 article reviews
murine cell line d3 - by Bioz Stars, 2026-08
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mouse embryonic stem mes cell line d3  (ATCC)
96
ATCC mouse embryonic stem mes cell line d3
Gli2 and MEF2C are expressed <t>during</t> <t>mES</t> differentiation. ( A ) Schematic representation of cardiomyogenesis in <t>D3</t> mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.
Mouse Embryonic Stem Mes Cell Line D3, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mes+d3+cells/Staphylococcus%3B+aureus%3B+subsp%2E+aureus/pmc06992348-48-0-18
Average 96 stars, based on 1 article reviews
mouse embryonic stem mes cell line d3 - by Bioz Stars, 2026-08
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d3 obtained from american type culture collection cat crl 11632 mes cell lines  (ATCC)
97
ATCC d3 obtained from american type culture collection cat crl 11632 mes cell lines
Gli2 and MEF2C are expressed <t>during</t> <t>mES</t> differentiation. ( A ) Schematic representation of cardiomyogenesis in <t>D3</t> mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.
D3 Obtained From American Type Culture Collection Cat Crl 11632 Mes Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mes+d3+cells/TIBx%3B+Epithelial+liver%3B+Mouse/pmc03565968-105-17-20
Average 97 stars, based on 1 article reviews
d3 obtained from american type culture collection cat crl 11632 mes cell lines - by Bioz Stars, 2026-08
97/100 stars
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stem cells mes cells  (ATCC)
96
ATCC stem cells mes cells
Gli2 and MEF2C are expressed <t>during</t> <t>mES</t> differentiation. ( A ) Schematic representation of cardiomyogenesis in <t>D3</t> mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.
Stem Cells Mes Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mes+d3+cells/Mouse+ES+Cell+Basal+Medium/us10711307-282-35-41
Average 96 stars, based on 1 article reviews
stem cells mes cells - by Bioz Stars, 2026-08
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d3 mouse embryonic stem cells mes  (ATCC)
92
ATCC d3 mouse embryonic stem cells mes
Target organs or systems for NMs in the present study: immune system (HMDM, RAW264.7 and MH-S), respiratory system (Calu-3, 16HBE and RLE-6TN), male reproductive system (TM3 and TM4), gastrointestinal system (Caco-2), kidneys (NRK-52E) and <t>embryo</t> <t>(NIH-3T3</t> and <t>mES/D3).</t>
D3 Mouse Embryonic Stem Cells Mes, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mes+d3+cells/Es-D3%3B+Embryonic+Stem+Cell%3B+Mouse/pmc04440714-402-4-13
Average 92 stars, based on 1 article reviews
d3 mouse embryonic stem cells mes - by Bioz Stars, 2026-08
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es-d3  (BioResource International Inc)
90
BioResource International Inc es-d3
Target organs or systems for NMs in the present study: immune system (HMDM, RAW264.7 and MH-S), respiratory system (Calu-3, 16HBE and RLE-6TN), male reproductive system (TM3 and TM4), gastrointestinal system (Caco-2), kidneys (NRK-52E) and <t>embryo</t> <t>(NIH-3T3</t> and <t>mES/D3).</t>
Es D3, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mes+d3+cells/murine+embryonic+pluripotent+stem+cells+es+d3/pmc04592426-63-9-13
Average 90 stars, based on 1 article reviews
es-d3 - by Bioz Stars, 2026-08
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mouse embryonic stem cells cell mes lines d3  (ATCC)
91
ATCC mouse embryonic stem cells cell mes lines d3
Target organs or systems for NMs in the present study: immune system (HMDM, RAW264.7 and MH-S), respiratory system (Calu-3, 16HBE and RLE-6TN), male reproductive system (TM3 and TM4), gastrointestinal system (Caco-2), kidneys (NRK-52E) and <t>embryo</t> <t>(NIH-3T3</t> and <t>mES/D3).</t>
Mouse Embryonic Stem Cells Cell Mes Lines D3, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mes+d3+cells/ES-D3%3B+Embryonic+Stem+Cell%3B+Mouse/pm36936789-218-18-26
Average 91 stars, based on 1 article reviews
mouse embryonic stem cells cell mes lines d3 - by Bioz Stars, 2026-08
91/100 stars
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Image Search Results


Gli2 and MEF2C are expressed during mES differentiation. ( A ) Schematic representation of cardiomyogenesis in D3 mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.

Journal: Nucleic Acids Research

Article Title: Gli2 and MEF2C activate each other's expression and function synergistically during cardiomyogenesis in vitro

doi: 10.1093/nar/gkr1232

Figure Lengend Snippet: Gli2 and MEF2C are expressed during mES differentiation. ( A ) Schematic representation of cardiomyogenesis in D3 mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.

Article Snippet: D3 mES cells (ATCC, # CRL-1934) were maintained as described in ref. ( , ).

Techniques: Expressing, Staining, Western Blot, Binding Assay, Control

Summary of gene expression changes in cell lines treated with or without DMSO

Journal: Nucleic Acids Research

Article Title: Gli2 and MEF2C activate each other's expression and function synergistically during cardiomyogenesis in vitro

doi: 10.1093/nar/gkr1232

Figure Lengend Snippet: Summary of gene expression changes in cell lines treated with or without DMSO

Article Snippet: D3 mES cells (ATCC, # CRL-1934) were maintained as described in ref. ( , ).

Techniques: Gene Expression

Target organs or systems for NMs in the present study: immune system (HMDM, RAW264.7 and MH-S), respiratory system (Calu-3, 16HBE and RLE-6TN), male reproductive system (TM3 and TM4), gastrointestinal system (Caco-2), kidneys (NRK-52E) and embryo (NIH-3T3 and mES/D3).

Journal: PLoS ONE

Article Title: Comprehensive In Vitro Toxicity Testing of a Panel of Representative Oxide Nanomaterials: First Steps towards an Intelligent Testing Strategy

doi: 10.1371/journal.pone.0127174

Figure Lengend Snippet: Target organs or systems for NMs in the present study: immune system (HMDM, RAW264.7 and MH-S), respiratory system (Calu-3, 16HBE and RLE-6TN), male reproductive system (TM3 and TM4), gastrointestinal system (Caco-2), kidneys (NRK-52E) and embryo (NIH-3T3 and mES/D3).

Article Snippet: Mouse NIH/3T3 cells and D3 mouse Embryonic Stem cells (mES) were purchased from ATCC (Manassas, VA, USA).

Techniques: