Journal: Journal of Virology

Article Title: Development of a Primary Human Cell Model for the Study of Human Cytomegalovirus Replication and Spread within Salivary Epithelium

doi: 10.1128/JVI.01608-18

Figure Lengend Snippet: Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on Cultrex PathClear basement membrane extract (BME) gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.

Article Snippet: Following digestion and lysis of red blood cells, the salivary cells were plated on Cultrex PathClear basement membrane extract (BME) gels (R&D Systems) and cultured in BEGM growth medium (containing the BEGM bullet kit and 4% charcoal-stripped fetal bovine serum [FBS]), provided by Lonza.

Techniques: Isolation, Cell Culture, Membrane, Infection, Expressing, Gene Expression, In Vitro, In Vivo

Journal: Foods

Article Title: Sea Buckthorn Pericarp Flavonoids Improve Diet-Induced Hyperlipidemia via Coordinated Modulation of Hepatic Lipid Metabolism and Gut Microbiota

doi: 10.3390/foods15061049

Figure Lengend Snippet: TFSP modulates the expression of ACC, FAS, CPT-1α, PPARα and ATGL proteins in the livers of HFD mice. n = 6 per group. Data are presented as mean ± SD. Different letters above bars indicate statistically significant differences ( p < 0.05) by one-way ANOVA followed by LSD post hoc test.

Article Snippet: Peroxisome proliferator-activated receptor alpha (PPARα) primary antibody (Cat. No. A00600-2), carnitine palmitoyltransferase-1 alpha (CPT-1α) primary antibody (Cat. No. A00917-3), acetyl-CoA carboxylase (ACC) primary antibody (Cat. No. M01802-2), fatty acid synthase (FAS) primary antibody (Cat. No. BA0484), adipose triglyceride lipase (ATGL) primary antibody (Cat. No. A01800-1), and GAPDH primary antibody (Cat. No. BM1623) were obtained from BOSTER Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing