med12 Search Results


gene exp med12 cg04504095 g1  (Thermo Fisher)
91
Thermo Fisher gene exp med12 cg04504095 g1
Gene Exp Med12 Cg04504095 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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med12  (Novus Biologicals)
91
Novus Biologicals med12
TET3 affects DNA methylation and histone modifications of the <t>MED12,</t> TGFBR2, and TSP1 promoters. a UtLM cells were transfected with siCon or siTET3 for 48 h, followed by ChIP-qPCR analysis. Data are presented as mean relative TET3 enrichment over input. n = 3. Red numbers indicate nucleotide positions relative to the transcriptional start sites, with PCR products depicted as red-stripped bars. b Sequences of critical transcription regulatory regions (CTRR) of MED12 , TGFBR2 , and TSP1 . The differentially methylated cytosine residues are marked in red. The red numbers mark the positions of the indicated nucleotides relative to the transcriptional start sites. c UtLM cells were transfected with siCon or siTET3 for 48 h, followed by QMSP analysis. n = 3. d UtLM cells were transfected with siCon or siTET3 for 48 h, followed by ChIP-qPCR analysis. Data are presented as mean relative enrichment over input. n = 3. All data are representative of at least two independent experiments and are presented as mean ± SEM. * p < 0.05, ** p < 0.01
Med12, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
med12 - by Bioz Stars, 2026-04
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med12  (Cell Signaling Technology Inc)
92
Cell Signaling Technology Inc med12
TET3 affects DNA methylation and histone modifications of the <t>MED12,</t> TGFBR2, and TSP1 promoters. a UtLM cells were transfected with siCon or siTET3 for 48 h, followed by ChIP-qPCR analysis. Data are presented as mean relative TET3 enrichment over input. n = 3. Red numbers indicate nucleotide positions relative to the transcriptional start sites, with PCR products depicted as red-stripped bars. b Sequences of critical transcription regulatory regions (CTRR) of MED12 , TGFBR2 , and TSP1 . The differentially methylated cytosine residues are marked in red. The red numbers mark the positions of the indicated nucleotides relative to the transcriptional start sites. c UtLM cells were transfected with siCon or siTET3 for 48 h, followed by QMSP analysis. n = 3. d UtLM cells were transfected with siCon or siTET3 for 48 h, followed by ChIP-qPCR analysis. Data are presented as mean relative enrichment over input. n = 3. All data are representative of at least two independent experiments and are presented as mean ± SEM. * p < 0.05, ** p < 0.01
Med12, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
med12 - by Bioz Stars, 2026-04
92/100 stars
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med12  (Bethyl)
93
Bethyl med12
(A) Co-IP experiment with antibodies against endogenous CDK8, MED1, and <t>MED12</t> in 293T whole cell lysis. The resultant immunoprecipitates were washed by washing buffers containing 0%, 5%, and 10% of 1,6-hexanediol respectively. Input: 0.5% of the total lysate was loaded. (B) Co-IP experiment with antibodies against endogenous CDK8, MED1, and MED12 in HeLa nuclear extract. The resultant immunoprecipitates were washed by washing buffers containing 10% of 1,6-hexanediol or 10% 2,5-hexanediol. Input: 0.2% of the total HeLa nuclear extract was loaded. (C) Co-IP experiment with antibodies against endogenous MED1 in 293T whole cell lysis. The resultant immunoprecipitated was washed by washing buffers containing 150 mM, 300 mM, and 500 mM of NaCl respectively. Input: 0.2% of the total HeLa nuclear extract was loaded. (D) Gel filtration chromatography of HeLa nuclear extract. 500µl HeLa nuclear extract was applied to Superose 6 column then was run in Buffer D. Column fractions of 500µl were collected. The Void (void volumn) of Superose 6 is based on the volume of effluent required for the elution of blue dextran (molecular mass of ∼2000 kDa). The molecular weight of corresponding fractions was detected by protein standards. (E) Immunoblots of anti-MED16 immunoprecipitation in HeLa nuclear extract and gel filtration factions No.29 to No.31. The immunoprecipitated proteins were detected with indicated antibodies by western blotting. HeLa NE input: 0.2% of the total HeLa nuclear extract was loaded. (F) Immunoblots of anti-TFCP2 immunoprecipitation in HeLa nuclear extract and gel filtration factions No.29 to No.31. The immunoprecipitated proteins were detected with indicated antibodies by western blotting. HeLa NE input: 0.2% of the total HeLa nuclear extract was loaded.
Med12, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
med12 - by Bioz Stars, 2026-04
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anti med12  (Proteintech)
93
Proteintech anti med12
(A) Co-IP experiment with antibodies against endogenous CDK8, MED1, and <t>MED12</t> in 293T whole cell lysis. The resultant immunoprecipitates were washed by washing buffers containing 0%, 5%, and 10% of 1,6-hexanediol respectively. Input: 0.5% of the total lysate was loaded. (B) Co-IP experiment with antibodies against endogenous CDK8, MED1, and MED12 in HeLa nuclear extract. The resultant immunoprecipitates were washed by washing buffers containing 10% of 1,6-hexanediol or 10% 2,5-hexanediol. Input: 0.2% of the total HeLa nuclear extract was loaded. (C) Co-IP experiment with antibodies against endogenous MED1 in 293T whole cell lysis. The resultant immunoprecipitated was washed by washing buffers containing 150 mM, 300 mM, and 500 mM of NaCl respectively. Input: 0.2% of the total HeLa nuclear extract was loaded. (D) Gel filtration chromatography of HeLa nuclear extract. 500µl HeLa nuclear extract was applied to Superose 6 column then was run in Buffer D. Column fractions of 500µl were collected. The Void (void volumn) of Superose 6 is based on the volume of effluent required for the elution of blue dextran (molecular mass of ∼2000 kDa). The molecular weight of corresponding fractions was detected by protein standards. (E) Immunoblots of anti-MED16 immunoprecipitation in HeLa nuclear extract and gel filtration factions No.29 to No.31. The immunoprecipitated proteins were detected with indicated antibodies by western blotting. HeLa NE input: 0.2% of the total HeLa nuclear extract was loaded. (F) Immunoblots of anti-TFCP2 immunoprecipitation in HeLa nuclear extract and gel filtration factions No.29 to No.31. The immunoprecipitated proteins were detected with indicated antibodies by western blotting. HeLa NE input: 0.2% of the total HeLa nuclear extract was loaded.
Anti Med12, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
anti med12 - by Bioz Stars, 2026-04
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med12 plx307  (Addgene inc)
91
Addgene inc med12 plx307
(A) Co-IP experiment with antibodies against endogenous CDK8, MED1, and <t>MED12</t> in 293T whole cell lysis. The resultant immunoprecipitates were washed by washing buffers containing 0%, 5%, and 10% of 1,6-hexanediol respectively. Input: 0.5% of the total lysate was loaded. (B) Co-IP experiment with antibodies against endogenous CDK8, MED1, and MED12 in HeLa nuclear extract. The resultant immunoprecipitates were washed by washing buffers containing 10% of 1,6-hexanediol or 10% 2,5-hexanediol. Input: 0.2% of the total HeLa nuclear extract was loaded. (C) Co-IP experiment with antibodies against endogenous MED1 in 293T whole cell lysis. The resultant immunoprecipitated was washed by washing buffers containing 150 mM, 300 mM, and 500 mM of NaCl respectively. Input: 0.2% of the total HeLa nuclear extract was loaded. (D) Gel filtration chromatography of HeLa nuclear extract. 500µl HeLa nuclear extract was applied to Superose 6 column then was run in Buffer D. Column fractions of 500µl were collected. The Void (void volumn) of Superose 6 is based on the volume of effluent required for the elution of blue dextran (molecular mass of ∼2000 kDa). The molecular weight of corresponding fractions was detected by protein standards. (E) Immunoblots of anti-MED16 immunoprecipitation in HeLa nuclear extract and gel filtration factions No.29 to No.31. The immunoprecipitated proteins were detected with indicated antibodies by western blotting. HeLa NE input: 0.2% of the total HeLa nuclear extract was loaded. (F) Immunoblots of anti-TFCP2 immunoprecipitation in HeLa nuclear extract and gel filtration factions No.29 to No.31. The immunoprecipitated proteins were detected with indicated antibodies by western blotting. HeLa NE input: 0.2% of the total HeLa nuclear extract was loaded.
Med12 Plx307, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
med12 plx307 - by Bioz Stars, 2026-04
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gene exp med12 hs00192801 m1  (Thermo Fisher)
90
Thermo Fisher gene exp med12 hs00192801 m1
(A) Co-IP experiment with antibodies against endogenous CDK8, MED1, and <t>MED12</t> in 293T whole cell lysis. The resultant immunoprecipitates were washed by washing buffers containing 0%, 5%, and 10% of 1,6-hexanediol respectively. Input: 0.5% of the total lysate was loaded. (B) Co-IP experiment with antibodies against endogenous CDK8, MED1, and MED12 in HeLa nuclear extract. The resultant immunoprecipitates were washed by washing buffers containing 10% of 1,6-hexanediol or 10% 2,5-hexanediol. Input: 0.2% of the total HeLa nuclear extract was loaded. (C) Co-IP experiment with antibodies against endogenous MED1 in 293T whole cell lysis. The resultant immunoprecipitated was washed by washing buffers containing 150 mM, 300 mM, and 500 mM of NaCl respectively. Input: 0.2% of the total HeLa nuclear extract was loaded. (D) Gel filtration chromatography of HeLa nuclear extract. 500µl HeLa nuclear extract was applied to Superose 6 column then was run in Buffer D. Column fractions of 500µl were collected. The Void (void volumn) of Superose 6 is based on the volume of effluent required for the elution of blue dextran (molecular mass of ∼2000 kDa). The molecular weight of corresponding fractions was detected by protein standards. (E) Immunoblots of anti-MED16 immunoprecipitation in HeLa nuclear extract and gel filtration factions No.29 to No.31. The immunoprecipitated proteins were detected with indicated antibodies by western blotting. HeLa NE input: 0.2% of the total HeLa nuclear extract was loaded. (F) Immunoblots of anti-TFCP2 immunoprecipitation in HeLa nuclear extract and gel filtration factions No.29 to No.31. The immunoprecipitated proteins were detected with indicated antibodies by western blotting. HeLa NE input: 0.2% of the total HeLa nuclear extract was loaded.
Gene Exp Med12 Hs00192801 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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gene exp med12 mm00804032 m1  (Thermo Fisher)
86
Thermo Fisher gene exp med12 mm00804032 m1
(A) Co-IP experiment with antibodies against endogenous CDK8, MED1, and <t>MED12</t> in 293T whole cell lysis. The resultant immunoprecipitates were washed by washing buffers containing 0%, 5%, and 10% of 1,6-hexanediol respectively. Input: 0.5% of the total lysate was loaded. (B) Co-IP experiment with antibodies against endogenous CDK8, MED1, and MED12 in HeLa nuclear extract. The resultant immunoprecipitates were washed by washing buffers containing 10% of 1,6-hexanediol or 10% 2,5-hexanediol. Input: 0.2% of the total HeLa nuclear extract was loaded. (C) Co-IP experiment with antibodies against endogenous MED1 in 293T whole cell lysis. The resultant immunoprecipitated was washed by washing buffers containing 150 mM, 300 mM, and 500 mM of NaCl respectively. Input: 0.2% of the total HeLa nuclear extract was loaded. (D) Gel filtration chromatography of HeLa nuclear extract. 500µl HeLa nuclear extract was applied to Superose 6 column then was run in Buffer D. Column fractions of 500µl were collected. The Void (void volumn) of Superose 6 is based on the volume of effluent required for the elution of blue dextran (molecular mass of ∼2000 kDa). The molecular weight of corresponding fractions was detected by protein standards. (E) Immunoblots of anti-MED16 immunoprecipitation in HeLa nuclear extract and gel filtration factions No.29 to No.31. The immunoprecipitated proteins were detected with indicated antibodies by western blotting. HeLa NE input: 0.2% of the total HeLa nuclear extract was loaded. (F) Immunoblots of anti-TFCP2 immunoprecipitation in HeLa nuclear extract and gel filtration factions No.29 to No.31. The immunoprecipitated proteins were detected with indicated antibodies by western blotting. HeLa NE input: 0.2% of the total HeLa nuclear extract was loaded.
Gene Exp Med12 Mm00804032 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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Image Search Results


TET3 affects DNA methylation and histone modifications of the MED12, TGFBR2, and TSP1 promoters. a UtLM cells were transfected with siCon or siTET3 for 48 h, followed by ChIP-qPCR analysis. Data are presented as mean relative TET3 enrichment over input. n = 3. Red numbers indicate nucleotide positions relative to the transcriptional start sites, with PCR products depicted as red-stripped bars. b Sequences of critical transcription regulatory regions (CTRR) of MED12 , TGFBR2 , and TSP1 . The differentially methylated cytosine residues are marked in red. The red numbers mark the positions of the indicated nucleotides relative to the transcriptional start sites. c UtLM cells were transfected with siCon or siTET3 for 48 h, followed by QMSP analysis. n = 3. d UtLM cells were transfected with siCon or siTET3 for 48 h, followed by ChIP-qPCR analysis. Data are presented as mean relative enrichment over input. n = 3. All data are representative of at least two independent experiments and are presented as mean ± SEM. * p < 0.05, ** p < 0.01

Journal: Oncogene

Article Title: H19 lncRNA identified as a master regulator of genes that drive uterine leiomyomas

doi: 10.1038/s41388-019-0808-4

Figure Lengend Snippet: TET3 affects DNA methylation and histone modifications of the MED12, TGFBR2, and TSP1 promoters. a UtLM cells were transfected with siCon or siTET3 for 48 h, followed by ChIP-qPCR analysis. Data are presented as mean relative TET3 enrichment over input. n = 3. Red numbers indicate nucleotide positions relative to the transcriptional start sites, with PCR products depicted as red-stripped bars. b Sequences of critical transcription regulatory regions (CTRR) of MED12 , TGFBR2 , and TSP1 . The differentially methylated cytosine residues are marked in red. The red numbers mark the positions of the indicated nucleotides relative to the transcriptional start sites. c UtLM cells were transfected with siCon or siTET3 for 48 h, followed by QMSP analysis. n = 3. d UtLM cells were transfected with siCon or siTET3 for 48 h, followed by ChIP-qPCR analysis. Data are presented as mean relative enrichment over input. n = 3. All data are representative of at least two independent experiments and are presented as mean ± SEM. * p < 0.05, ** p < 0.01

Article Snippet: Antibodies for TET3 (GeneTex, GTX121453; used at a dilution of 1/500), TGFBR2 (Abcam, ab184948; used at a dilution of 1/1000), TSP1 (Abcam, ab85762; used at a dilution of 1/500), MED12 (Novus Biological, NB100–2357; used at a dilution of 1/500), HMGA2 (Proteintech, 20795–1-AP; used at a dilution of 1/500), GRAF1 (Cell Signaling, 8802; used at a dilution of 1/500), SPARC (Cell Signaling, 8725; used at a dilution of 1/500), COL3A1 (LS-Bio, LS-C159386; used at a dilution of 1/1000), COL4A1 (LS-Bio, LS-C100552; used at a dilution of 1/500), COL5A2 (Origene, TA809611; used at a dilution of 1/500), and GAPDH (Abcam, ab128915; used at a dilution of 1/10000) were purchased.

Techniques: DNA Methylation Assay, Transfection, ChIP-qPCR, Methylation

H19 and TET3 co-express with fibroid-promoting genes in vivo. a , c RT-qPCR analyses were performed on RNAs extracted from human fibroids and matched myometrium tissues. Spearman’s correlation showed positive correlations between expression of H19 and TET3 ( a , left panel), as well as TET3 and its target genes MED12 , TGFBR2 , and TSP1 ( c ) in a statistically significant manner. No correlation between expression of H19 and HMGA2 at the RNA level was detected ( a , right panel). Spearman’s correlation coefficient, p -values, and sample numbers are presented. b Results of western blotting analysis of HMGA2 in human fibroids and matched myometrium. n = 3. Data are representative of two independent experiments and are presented as mean ± SEM

Journal: Oncogene

Article Title: H19 lncRNA identified as a master regulator of genes that drive uterine leiomyomas

doi: 10.1038/s41388-019-0808-4

Figure Lengend Snippet: H19 and TET3 co-express with fibroid-promoting genes in vivo. a , c RT-qPCR analyses were performed on RNAs extracted from human fibroids and matched myometrium tissues. Spearman’s correlation showed positive correlations between expression of H19 and TET3 ( a , left panel), as well as TET3 and its target genes MED12 , TGFBR2 , and TSP1 ( c ) in a statistically significant manner. No correlation between expression of H19 and HMGA2 at the RNA level was detected ( a , right panel). Spearman’s correlation coefficient, p -values, and sample numbers are presented. b Results of western blotting analysis of HMGA2 in human fibroids and matched myometrium. n = 3. Data are representative of two independent experiments and are presented as mean ± SEM

Article Snippet: Antibodies for TET3 (GeneTex, GTX121453; used at a dilution of 1/500), TGFBR2 (Abcam, ab184948; used at a dilution of 1/1000), TSP1 (Abcam, ab85762; used at a dilution of 1/500), MED12 (Novus Biological, NB100–2357; used at a dilution of 1/500), HMGA2 (Proteintech, 20795–1-AP; used at a dilution of 1/500), GRAF1 (Cell Signaling, 8802; used at a dilution of 1/500), SPARC (Cell Signaling, 8725; used at a dilution of 1/500), COL3A1 (LS-Bio, LS-C159386; used at a dilution of 1/1000), COL4A1 (LS-Bio, LS-C100552; used at a dilution of 1/500), COL5A2 (Origene, TA809611; used at a dilution of 1/500), and GAPDH (Abcam, ab128915; used at a dilution of 1/10000) were purchased.

Techniques: In Vivo, Quantitative RT-PCR, Expressing, Western Blot

(A) Co-IP experiment with antibodies against endogenous CDK8, MED1, and MED12 in 293T whole cell lysis. The resultant immunoprecipitates were washed by washing buffers containing 0%, 5%, and 10% of 1,6-hexanediol respectively. Input: 0.5% of the total lysate was loaded. (B) Co-IP experiment with antibodies against endogenous CDK8, MED1, and MED12 in HeLa nuclear extract. The resultant immunoprecipitates were washed by washing buffers containing 10% of 1,6-hexanediol or 10% 2,5-hexanediol. Input: 0.2% of the total HeLa nuclear extract was loaded. (C) Co-IP experiment with antibodies against endogenous MED1 in 293T whole cell lysis. The resultant immunoprecipitated was washed by washing buffers containing 150 mM, 300 mM, and 500 mM of NaCl respectively. Input: 0.2% of the total HeLa nuclear extract was loaded. (D) Gel filtration chromatography of HeLa nuclear extract. 500µl HeLa nuclear extract was applied to Superose 6 column then was run in Buffer D. Column fractions of 500µl were collected. The Void (void volumn) of Superose 6 is based on the volume of effluent required for the elution of blue dextran (molecular mass of ∼2000 kDa). The molecular weight of corresponding fractions was detected by protein standards. (E) Immunoblots of anti-MED16 immunoprecipitation in HeLa nuclear extract and gel filtration factions No.29 to No.31. The immunoprecipitated proteins were detected with indicated antibodies by western blotting. HeLa NE input: 0.2% of the total HeLa nuclear extract was loaded. (F) Immunoblots of anti-TFCP2 immunoprecipitation in HeLa nuclear extract and gel filtration factions No.29 to No.31. The immunoprecipitated proteins were detected with indicated antibodies by western blotting. HeLa NE input: 0.2% of the total HeLa nuclear extract was loaded.

Journal: bioRxiv

Article Title: Mediator Subunit MED16 Collaborates with UBP1-TFCP2 to Control Transcriptional Activation or Repression via Promoter Positional Specificity

doi: 10.1101/2025.08.12.669905

Figure Lengend Snippet: (A) Co-IP experiment with antibodies against endogenous CDK8, MED1, and MED12 in 293T whole cell lysis. The resultant immunoprecipitates were washed by washing buffers containing 0%, 5%, and 10% of 1,6-hexanediol respectively. Input: 0.5% of the total lysate was loaded. (B) Co-IP experiment with antibodies against endogenous CDK8, MED1, and MED12 in HeLa nuclear extract. The resultant immunoprecipitates were washed by washing buffers containing 10% of 1,6-hexanediol or 10% 2,5-hexanediol. Input: 0.2% of the total HeLa nuclear extract was loaded. (C) Co-IP experiment with antibodies against endogenous MED1 in 293T whole cell lysis. The resultant immunoprecipitated was washed by washing buffers containing 150 mM, 300 mM, and 500 mM of NaCl respectively. Input: 0.2% of the total HeLa nuclear extract was loaded. (D) Gel filtration chromatography of HeLa nuclear extract. 500µl HeLa nuclear extract was applied to Superose 6 column then was run in Buffer D. Column fractions of 500µl were collected. The Void (void volumn) of Superose 6 is based on the volume of effluent required for the elution of blue dextran (molecular mass of ∼2000 kDa). The molecular weight of corresponding fractions was detected by protein standards. (E) Immunoblots of anti-MED16 immunoprecipitation in HeLa nuclear extract and gel filtration factions No.29 to No.31. The immunoprecipitated proteins were detected with indicated antibodies by western blotting. HeLa NE input: 0.2% of the total HeLa nuclear extract was loaded. (F) Immunoblots of anti-TFCP2 immunoprecipitation in HeLa nuclear extract and gel filtration factions No.29 to No.31. The immunoprecipitated proteins were detected with indicated antibodies by western blotting. HeLa NE input: 0.2% of the total HeLa nuclear extract was loaded.

Article Snippet: Antibody for Western blot include the following MED23 (Abcam, ab200351), MED1 (Bethyl Lab, A300-793A), MED24 (Bethyl, A301-472A), MED6 (Santa Cruz, sc-9434), MED12 (Bethyl Lab, A300-774A), MED16 (Bethyl Lab, A303-668A), CDK8 (Abcam, ab115155), UBP1 (Protientech, 67318-1-Ig), TFCP2 (Proteintech, 15203-1-AP), YY1 (Proteintech, 66281-1-Ig), P300 (Abcam, ab14984), HDAC1 (Santa Cruz, sc-7872), PolII (Abcam, ab816), PolII-pSer5 (Millipore, 04-1572), TFIIB (Santa Cruz, sc-274D), TFIIH (Santa Cruz, sc-293), H3 (Abcam, ab1791), H3ac (Abcam, ab4729), β-Actin (Proteintech, 66009-1-Ig), GAPDH (Proteintech, 60004-1-Ig), Flag-tag (Sigma-Aldrich, F1804), His-tag (Proteintech, 66005-1-Ig), GST-tag (Invitrogen, 10004D).

Techniques: Co-Immunoprecipitation Assay, Lysis, Immunoprecipitation, Filtration, Chromatography, Molecular Weight, Western Blot

Venn diagram of MED1, MED12, CDK8 and MED16 interacting proteins identified by IP-MS. MED16 specific interacting proteins were highlight on the right.

Journal: bioRxiv

Article Title: Mediator Subunit MED16 Collaborates with UBP1-TFCP2 to Control Transcriptional Activation or Repression via Promoter Positional Specificity

doi: 10.1101/2025.08.12.669905

Figure Lengend Snippet: Venn diagram of MED1, MED12, CDK8 and MED16 interacting proteins identified by IP-MS. MED16 specific interacting proteins were highlight on the right.

Article Snippet: Antibody for Western blot include the following MED23 (Abcam, ab200351), MED1 (Bethyl Lab, A300-793A), MED24 (Bethyl, A301-472A), MED6 (Santa Cruz, sc-9434), MED12 (Bethyl Lab, A300-774A), MED16 (Bethyl Lab, A303-668A), CDK8 (Abcam, ab115155), UBP1 (Protientech, 67318-1-Ig), TFCP2 (Proteintech, 15203-1-AP), YY1 (Proteintech, 66281-1-Ig), P300 (Abcam, ab14984), HDAC1 (Santa Cruz, sc-7872), PolII (Abcam, ab816), PolII-pSer5 (Millipore, 04-1572), TFIIB (Santa Cruz, sc-274D), TFIIH (Santa Cruz, sc-293), H3 (Abcam, ab1791), H3ac (Abcam, ab4729), β-Actin (Proteintech, 66009-1-Ig), GAPDH (Proteintech, 60004-1-Ig), Flag-tag (Sigma-Aldrich, F1804), His-tag (Proteintech, 66005-1-Ig), GST-tag (Invitrogen, 10004D).

Techniques: Protein-Protein interactions