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  • 86
    Becton Dickinson myeloid derived suppressor cells
    Myeloid Derived Suppressor Cells, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/myeloid derived suppressor cells/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    myeloid derived suppressor cells - by Bioz Stars, 2021-09
    86/100 stars
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    86
    Thermo Fisher g mdscs
    Knockdown of <t>Pvt1</t> alters the suppressive capacity of <t>G-MDSCs</t> in vitro. G-MDSCs sorted from tumor tissues were obtained from TB mice injected with cells transfected with 50 nM Pvt1 siRNA (si-Pvt1) or negative control (NC) siRNA (si-NC). a qRT-PCR confirmed the efficiency of transfection with si-Pvt1. b G-MDSCs were transfected with Pvt1 siRNA, and then, the cells were harvested after 6 h and cocultured with CD4 + T cells at ratio of 1:1 in the presence of anti-CD3 mAb and anti-CD28 mAb for72 h. 3H-thymidine incorporation was used to detect T cells proliferation. c Arg1 activity in G-MDSCs transfected with si-Pvt1 was measured. d ROS production in G-MDSCs was analyzed via flow cytometry. ** p
    G Mdscs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g mdscs/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    g mdscs - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    86
    Miltenyi Biotec myeloid derived suppressor cell isolation kit
    Knockdown of <t>Pvt1</t> alters the suppressive capacity of <t>G-MDSCs</t> in vitro. G-MDSCs sorted from tumor tissues were obtained from TB mice injected with cells transfected with 50 nM Pvt1 siRNA (si-Pvt1) or negative control (NC) siRNA (si-NC). a qRT-PCR confirmed the efficiency of transfection with si-Pvt1. b G-MDSCs were transfected with Pvt1 siRNA, and then, the cells were harvested after 6 h and cocultured with CD4 + T cells at ratio of 1:1 in the presence of anti-CD3 mAb and anti-CD28 mAb for72 h. 3H-thymidine incorporation was used to detect T cells proliferation. c Arg1 activity in G-MDSCs transfected with si-Pvt1 was measured. d ROS production in G-MDSCs was analyzed via flow cytometry. ** p
    Myeloid Derived Suppressor Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/myeloid derived suppressor cell isolation kit/product/Miltenyi Biotec
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    myeloid derived suppressor cell isolation kit - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec mdscs
    Knockdown of <t>Pvt1</t> alters the suppressive capacity of <t>G-MDSCs</t> in vitro. G-MDSCs sorted from tumor tissues were obtained from TB mice injected with cells transfected with 50 nM Pvt1 siRNA (si-Pvt1) or negative control (NC) siRNA (si-NC). a qRT-PCR confirmed the efficiency of transfection with si-Pvt1. b G-MDSCs were transfected with Pvt1 siRNA, and then, the cells were harvested after 6 h and cocultured with CD4 + T cells at ratio of 1:1 in the presence of anti-CD3 mAb and anti-CD28 mAb for72 h. 3H-thymidine incorporation was used to detect T cells proliferation. c Arg1 activity in G-MDSCs transfected with si-Pvt1 was measured. d ROS production in G-MDSCs was analyzed via flow cytometry. ** p
    Mdscs, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mdscs/product/Miltenyi Biotec
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mdscs - by Bioz Stars, 2021-09
    96/100 stars
      Buy from Supplier

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    Knockdown of Pvt1 alters the suppressive capacity of G-MDSCs in vitro. G-MDSCs sorted from tumor tissues were obtained from TB mice injected with cells transfected with 50 nM Pvt1 siRNA (si-Pvt1) or negative control (NC) siRNA (si-NC). a qRT-PCR confirmed the efficiency of transfection with si-Pvt1. b G-MDSCs were transfected with Pvt1 siRNA, and then, the cells were harvested after 6 h and cocultured with CD4 + T cells at ratio of 1:1 in the presence of anti-CD3 mAb and anti-CD28 mAb for72 h. 3H-thymidine incorporation was used to detect T cells proliferation. c Arg1 activity in G-MDSCs transfected with si-Pvt1 was measured. d ROS production in G-MDSCs was analyzed via flow cytometry. ** p

    Journal: Molecular Cancer

    Article Title: Long noncoding RNA Pvt1 regulates the immunosuppression activity of granulocytic myeloid-derived suppressor cells in tumor-bearing mice

    doi: 10.1186/s12943-019-0978-2

    Figure Lengend Snippet: Knockdown of Pvt1 alters the suppressive capacity of G-MDSCs in vitro. G-MDSCs sorted from tumor tissues were obtained from TB mice injected with cells transfected with 50 nM Pvt1 siRNA (si-Pvt1) or negative control (NC) siRNA (si-NC). a qRT-PCR confirmed the efficiency of transfection with si-Pvt1. b G-MDSCs were transfected with Pvt1 siRNA, and then, the cells were harvested after 6 h and cocultured with CD4 + T cells at ratio of 1:1 in the presence of anti-CD3 mAb and anti-CD28 mAb for72 h. 3H-thymidine incorporation was used to detect T cells proliferation. c Arg1 activity in G-MDSCs transfected with si-Pvt1 was measured. d ROS production in G-MDSCs was analyzed via flow cytometry. ** p

    Article Snippet: However, whether Pvt1 regulates cell cycle progression and apoptosis in G-MDSCs requires further research.

    Techniques: In Vitro, Mouse Assay, Injection, Transfection, Negative Control, Quantitative RT-PCR, Activity Assay, Flow Cytometry, Cytometry

    Pvt1 knockdown reduces the ability of G-MDSCs to accelerate tumor progression and inhibit antitumor immune responses. Two groups of mice were given a s.c. injection of a mixture of LLCs and G-MDSCs transfected with si-Pvt1 (si-Pvt1 group) or si-NC (si-NC group). a Tumor volume was measured at the indicated time. b , c The proportions of CD8 + IFN-γ + CTLs and CD4 + IFN-γ + Th1 cells from draining lymph nodes, spleens and tumor tissues were analyzed via flow cytometry. ** p

    Journal: Molecular Cancer

    Article Title: Long noncoding RNA Pvt1 regulates the immunosuppression activity of granulocytic myeloid-derived suppressor cells in tumor-bearing mice

    doi: 10.1186/s12943-019-0978-2

    Figure Lengend Snippet: Pvt1 knockdown reduces the ability of G-MDSCs to accelerate tumor progression and inhibit antitumor immune responses. Two groups of mice were given a s.c. injection of a mixture of LLCs and G-MDSCs transfected with si-Pvt1 (si-Pvt1 group) or si-NC (si-NC group). a Tumor volume was measured at the indicated time. b , c The proportions of CD8 + IFN-γ + CTLs and CD4 + IFN-γ + Th1 cells from draining lymph nodes, spleens and tumor tissues were analyzed via flow cytometry. ** p

    Article Snippet: However, whether Pvt1 regulates cell cycle progression and apoptosis in G-MDSCs requires further research.

    Techniques: Mouse Assay, Injection, Transfection, Flow Cytometry, Cytometry

    c-myc is a potential downstream target of Pvt1 in G-MDSCs. a Scatter plot for protein-coding RNAs. b qRT-PCR was used to detect the mRNA level of c-myc in G-MDSCs sorted from spleen of WT mice and tumor tissues of TB mice. c , d After transfection with si-Pvt1, the mRNA and protein levels of c-myc in G-MDSCs isolated from tumor tissues were measured via qRT-PCR and western blot analyses. ** p

    Journal: Molecular Cancer

    Article Title: Long noncoding RNA Pvt1 regulates the immunosuppression activity of granulocytic myeloid-derived suppressor cells in tumor-bearing mice

    doi: 10.1186/s12943-019-0978-2

    Figure Lengend Snippet: c-myc is a potential downstream target of Pvt1 in G-MDSCs. a Scatter plot for protein-coding RNAs. b qRT-PCR was used to detect the mRNA level of c-myc in G-MDSCs sorted from spleen of WT mice and tumor tissues of TB mice. c , d After transfection with si-Pvt1, the mRNA and protein levels of c-myc in G-MDSCs isolated from tumor tissues were measured via qRT-PCR and western blot analyses. ** p

    Article Snippet: However, whether Pvt1 regulates cell cycle progression and apoptosis in G-MDSCs requires further research.

    Techniques: Quantitative RT-PCR, Mouse Assay, Transfection, Isolation, Western Blot

    Pvt1 is highly expressed in tumor-expanded G-MDSCs. A total of 2 × 10 ^6 Lewis lung carcinoma cells (LLCs) were introduced via s.c. injection into C57BL/6 mice. After 4 weeks, bone marrow cells, splenocytes and a single-cell suspension derived from tumor tissues were collected, and G-MDSCs were later sorted. Splenocytes from wild-type (WT) C57BL/6 mice were collected, and G-MDSCs were isolated. Hierarchical clustering analysis of lncRNAs and protein-coding RNAs that were differentially expressed (fold change > 2) in G-MDSCs sorted from tumor tissue of Lewis tumor-bearing mice and spleens of WT C57BL/6 mice. a Clustering tree for lncRNAs; the expression values are represented in shades of red and green, indicating expression above and below normal values, respectively. b The purity of sorted G-MDSCs was determined via flow cytometry by assessing the expression of two surface markers: Ly6G and CD11b. c The expression level of Pvt1 in total RNA isolated from G-MDSCs from the bone marrow, spleen and tumor tissues of Lewis-bearing mice was measured by qRT-PCR. Fresh G-MDSCs isolated from bone marrow (BM) from WT C57BL/6 mice served as the control. Bone marrow cells (1 × 10 ^6 ) from WT C57BL/6 mice were plated in 24-well plates in 1 mL of RPMI 1640 medium containing 10% FBS, 20 ng/mL IL-6 and 20 ng/mL GM-CSF. The cells were then collected, and G-MDSCs were sorted 3 days later. d G-MDSCs cocultured with CFSE-labeled CD4 + T cells at a ratio of 1:1 in the presence of anti-CD3 mAb and anti-CD28 mAb for 72 h. The proliferation of CD4 + T cells was detected by flow cytometry at 488 nm excitation light. e Arg1 activity in G-MDSCs induced from BM cells was measured. f ROS production in G-MDSCs was analyzed via flow cytometry. g The expression level of Pvt1 in G-MDSCs was detected using qRT-PCR. *** p

    Journal: Molecular Cancer

    Article Title: Long noncoding RNA Pvt1 regulates the immunosuppression activity of granulocytic myeloid-derived suppressor cells in tumor-bearing mice

    doi: 10.1186/s12943-019-0978-2

    Figure Lengend Snippet: Pvt1 is highly expressed in tumor-expanded G-MDSCs. A total of 2 × 10 ^6 Lewis lung carcinoma cells (LLCs) were introduced via s.c. injection into C57BL/6 mice. After 4 weeks, bone marrow cells, splenocytes and a single-cell suspension derived from tumor tissues were collected, and G-MDSCs were later sorted. Splenocytes from wild-type (WT) C57BL/6 mice were collected, and G-MDSCs were isolated. Hierarchical clustering analysis of lncRNAs and protein-coding RNAs that were differentially expressed (fold change > 2) in G-MDSCs sorted from tumor tissue of Lewis tumor-bearing mice and spleens of WT C57BL/6 mice. a Clustering tree for lncRNAs; the expression values are represented in shades of red and green, indicating expression above and below normal values, respectively. b The purity of sorted G-MDSCs was determined via flow cytometry by assessing the expression of two surface markers: Ly6G and CD11b. c The expression level of Pvt1 in total RNA isolated from G-MDSCs from the bone marrow, spleen and tumor tissues of Lewis-bearing mice was measured by qRT-PCR. Fresh G-MDSCs isolated from bone marrow (BM) from WT C57BL/6 mice served as the control. Bone marrow cells (1 × 10 ^6 ) from WT C57BL/6 mice were plated in 24-well plates in 1 mL of RPMI 1640 medium containing 10% FBS, 20 ng/mL IL-6 and 20 ng/mL GM-CSF. The cells were then collected, and G-MDSCs were sorted 3 days later. d G-MDSCs cocultured with CFSE-labeled CD4 + T cells at a ratio of 1:1 in the presence of anti-CD3 mAb and anti-CD28 mAb for 72 h. The proliferation of CD4 + T cells was detected by flow cytometry at 488 nm excitation light. e Arg1 activity in G-MDSCs induced from BM cells was measured. f ROS production in G-MDSCs was analyzed via flow cytometry. g The expression level of Pvt1 in G-MDSCs was detected using qRT-PCR. *** p

    Article Snippet: However, whether Pvt1 regulates cell cycle progression and apoptosis in G-MDSCs requires further research.

    Techniques: Injection, Mouse Assay, Derivative Assay, Isolation, Expressing, Flow Cytometry, Cytometry, Quantitative RT-PCR, Labeling, Activity Assay

    HIF-1α upregulates Pvt1 expression in G-MDSCs under hypoxic stress. a The mRNA level of HIF-1α in G-MDSCs sorted from spleens and tumor tissues of TB mice was detected using qRT-PCR. G-MDSCs isolated from spleens of TB mice were cultured in an incubator at 37 °C (20% O 2 , 5% CO 2 ) (normoxic conditions) or in a sealed box containing an anaerobic bag to consume oxygen (O 2

    Journal: Molecular Cancer

    Article Title: Long noncoding RNA Pvt1 regulates the immunosuppression activity of granulocytic myeloid-derived suppressor cells in tumor-bearing mice

    doi: 10.1186/s12943-019-0978-2

    Figure Lengend Snippet: HIF-1α upregulates Pvt1 expression in G-MDSCs under hypoxic stress. a The mRNA level of HIF-1α in G-MDSCs sorted from spleens and tumor tissues of TB mice was detected using qRT-PCR. G-MDSCs isolated from spleens of TB mice were cultured in an incubator at 37 °C (20% O 2 , 5% CO 2 ) (normoxic conditions) or in a sealed box containing an anaerobic bag to consume oxygen (O 2

    Article Snippet: However, whether Pvt1 regulates cell cycle progression and apoptosis in G-MDSCs requires further research.

    Techniques: Expressing, Mouse Assay, Quantitative RT-PCR, Isolation, Cell Culture