Journal: The Journal of infectious diseases

Article Title: Aberrant Humoral Immune Responses in Neurosyphilis: CXCL13/CXCR5 Play a Pivotal Role for B-Cell Recruitment to the Cerebrospinal Fluid.

doi: 10.1093/infdis/jix233

Figure Lengend Snippet: Figure 1. Enrichment of CD19+ B cells and memory B cells in the CSF of NS patients. A, Gating strategies. B, Representative flow cytometry analysis of the frequency of CSF total CD19+ B cells and subsets of CD19+ B cells between non-NS and NS patients (first row). Bar charts of the frequency of B-cell subsets of patients with asymptomatic and symptomatic NS (second row). Comparison of the proportion of CSF total CXCR5+ B cells in different groups (third row). C, Serial changes of CSF total CD19+ B cells in the patient with NS following first and second treatment. D, Correlation of frequency of CD19+ B cells and CSF protein levels, CSF WBC counts, or CSF VDRL titer. Abbreviations: CSF, cerebrospinal fluid; NS, neurosyphilis; VDRL, Venereal Disease Research Laboratory; WBC, white blood cell.

Article Snippet: Paraffin-embedded brain biopsy (4 μm) was stained with appropriate concentration of primary antibodies against CXCL13, CXCR5, CD20, CD3, CD35, CD138 (Maixin-Bio, Fujian, China), and spirochete (Biocare Medical, Pacheco, CA), then incubated with streptavidin-biotin complex system according to manufacturer’s instructions (Boster, Pleasanton, CA).

Techniques: Flow Cytometry, Comparison

Journal: The Journal of infectious diseases

Article Title: Aberrant Humoral Immune Responses in Neurosyphilis: CXCL13/CXCR5 Play a Pivotal Role for B-Cell Recruitment to the Cerebrospinal Fluid.

doi: 10.1093/infdis/jix233

Figure Lengend Snippet: Figure 5. Pathologic diagnosis of intracranial syphilitic gumma and cellular organization of follicle-like structures. HE staining of intracranial syphilitic gumma (first row). Immunostaining of spirochete in intracranial syphilitic gumma (second row). Immunostaining of CXCL13 in intracranial syphilitic gumma and follicle-like structure (third row). Immunostaining of CXCR5 in intracranial syphilitic gumma and follicle-like structure (fourth row). Immunostaining of CD20+ B cells in intra- cranial syphilitic gumma and follicle-like structure (fifth row). Immunostaining of CD3+ T cells in intracranial syphilitic gumma and follicle-like structure (sixth row). Immunostaining of CD35+ FDCs in intracranial syphilitic gumma and follicle–like structure (seventh row). Immunostaining of CD138+ plasma cells in intracranial syphilitic gumma and follicle-like structure (eighth row). Abbreviations: FDCs, follic- ular dendritic cells; HE, hematoxylin and eosin.

Article Snippet: Paraffin-embedded brain biopsy (4 μm) was stained with appropriate concentration of primary antibodies against CXCL13, CXCR5, CD20, CD3, CD35, CD138 (Maixin-Bio, Fujian, China), and spirochete (Biocare Medical, Pacheco, CA), then incubated with streptavidin-biotin complex system according to manufacturer’s instructions (Boster, Pleasanton, CA).

Techniques: Biomarker Discovery, Staining, Immunostaining, Clinical Proteomics

Journal: Frontiers in Pharmacology

Article Title: Mechanism of Paeoniflorin on ANIT-Induced Cholestatic Liver Injury Using Integrated Metabolomics and Network Pharmacology

doi: 10.3389/fphar.2021.737630

Figure Lengend Snippet: Effect of PF on the expression of CYP2C9, ABCB1, MAOB, CDC25B, and MTOR in the treatment of cholestatic liver injury detected by western blotting. (A) Western blotting images of CYP2C9, ABCB1, MAOB, CDC25B, and MTOR; (B) Relative protein expression of CDC25B; (C) Relative protein expression of CYP2C9; (D) Relative protein expression of MAOB; (E) Relative protein expression of ABCB1; (F) Relative protein expression of MTOR. The data are expressed as the mean ± SD, n = 3. ## p < 0.01, # p < 0.05 compared with the control group, ** p < 0.01, * p < 0.05 compared with ANIT group.

Article Snippet: The PVDF membranes were blocked with 5% fat-free milk at room temperature for 2 hours, then incubated overnight at 4°C with antibodies against anit-CDC25B (PB9488, BOSTER, dilution: 1:1,000), anit-MTOR (A00003-2, BOSTER, dilution: 1:1,000), anit-CYP2C9 (16546-1-AP, proteintech, dilution: 1:2,000), anit-ABCB1 (A00049-3, BOSTER, dilution: 1:1,000), anit-MAOB (PB9665, BOSTER, dilution: 1:1,000).

Techniques: Expressing, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Mechanism of Paeoniflorin on ANIT-Induced Cholestatic Liver Injury Using Integrated Metabolomics and Network Pharmacology

doi: 10.3389/fphar.2021.737630

Figure Lengend Snippet: Effect of PF on the expression of CYP2C9, ABCB1, MAOB, CDC25B, and MTOR in the treatment of cholestatic liver injury. (A) The expression of CYP2C9 in the liver tissue; (B) The expression of ABCB1 in the liver tissue; (C) The expression of MAOB in the liver tissue; (D) The expression of CDC25B in the liver tissue; (E) The expression of MTOR in the liver tissue. The data are expressed as the mean ± SD, n = 3. ## p < 0.01, # p < 0.05 compared with the control group, ** p < 0.01, * p < 0.05 compared with ANIT group.

Article Snippet: The PVDF membranes were blocked with 5% fat-free milk at room temperature for 2 hours, then incubated overnight at 4°C with antibodies against anit-CDC25B (PB9488, BOSTER, dilution: 1:1,000), anit-MTOR (A00003-2, BOSTER, dilution: 1:1,000), anit-CYP2C9 (16546-1-AP, proteintech, dilution: 1:2,000), anit-ABCB1 (A00049-3, BOSTER, dilution: 1:1,000), anit-MAOB (PB9665, BOSTER, dilution: 1:1,000).

Techniques: Expressing, Control