Journal: PLoS ONE

Article Title: 3-O-Galloylated Procyanidins from Rumex acetosa L. Inhibit the Attachment of Influenza A Virus

doi: 10.1371/journal.pone.0110089

Figure Lengend Snippet: IAV and test compounds were co-incubated for 1 h at 37°C prior to the addition to MDCK II cells. Heparin served as positive control (D). Values (% of plaque reduction) ±SD relate to the respective mock-treated controls ( = 100%). * p<0.05, ** p<0.01 (two-tailed, unpaired Student's t-test).

Article Snippet: MDCK II cells (canine kidney cells) were propagated in minimal essential medium (MEM; Biochrom, Berlin, Germany) supplemented with 5% fetal calf serum (FCS; Biochrom), 2 mM l -glutamine (Sigma-Aldrich), non-essential amino acids (1×) and 100 μg/mL penicillin/streptomycin (Biochrom).

Techniques: Incubation, Positive Control, Two Tailed Test

Journal: PLoS ONE

Article Title: 3-O-Galloylated Procyanidins from Rumex acetosa L. Inhibit the Attachment of Influenza A Virus

doi: 10.1371/journal.pone.0110089

Figure Lengend Snippet: Effects on the penetration of IAV were determined by a modified plaque reduction assay. Test compounds were added for 30 min. after attachment of IAV to MDCK II cells at 4°C. Values (% of plaque reduction) ±SD relate to the respective mock-treated controls ( = 100%) and represent ≥3 independent experiments. * p<0.05, ** p<0.01 (two-tailed, unpaired Student's t-test).

Article Snippet: MDCK II cells (canine kidney cells) were propagated in minimal essential medium (MEM; Biochrom, Berlin, Germany) supplemented with 5% fetal calf serum (FCS; Biochrom), 2 mM l -glutamine (Sigma-Aldrich), non-essential amino acids (1×) and 100 μg/mL penicillin/streptomycin (Biochrom).

Techniques: Modification, Two Tailed Test

Journal: International Journal of Molecular Sciences

Article Title: A Unique In Vitro Assay to Investigate ABCB4 Transport Function

doi: 10.3390/ijms24054459

Figure Lengend Snippet: Efflux ratios for prototypic ABCB1 substrates. ( a ) Efflux ratios for digoxin and talinolol (1 µM, traced with 0.17 µCi/mL 3 H-labelled probe) in the MDCKII parental and in the Abcb1KO cells. The dashed line indicates an efflux ratio of 2. Data are presented as mean ± standard deviation for 3 experiments each performed in triplicate. ( b ) Functional characterization of ABCB1, Mock and ABCB4 cells using prototypical ABCB1 substrates (1 µM, traced with 0.17 µCi/mL 3 H-labelled probe). The dashed line indicates an efflux ratio of 2. Data are presented as mean ± standard deviation for 3 experiments each performed in triplicate.

Article Snippet: An Abcb1 biallelic knockout MDCKII (Abcb1KO-MDCKII) cell clone was generated by GenScript (Leiden, the Netherlands) using the CRISPR/Cas9 system.

Techniques: Standard Deviation, Functional Assay

Journal: International Journal of Molecular Sciences

Article Title: A Unique In Vitro Assay to Investigate ABCB4 Transport Function

doi: 10.3390/ijms24054459

Figure Lengend Snippet: Tested compounds with ABCB4 inhibition activities and with ABCB1 interaction abilities.

Article Snippet: An Abcb1 biallelic knockout MDCKII (Abcb1KO-MDCKII) cell clone was generated by GenScript (Leiden, the Netherlands) using the CRISPR/Cas9 system.

Techniques: Inhibition, In Vitro

Journal: International Journal of Molecular Sciences

Article Title: A Unique In Vitro Assay to Investigate ABCB4 Transport Function

doi: 10.3390/ijms24054459

Figure Lengend Snippet: Transcellular transport of TKIs in the ABCB4 and Mock cells. ( a ) Bidirectional transport of gefitinib. ABCB4 and Mock cells were incubated with 0.5 µM gefitinib for 60, 120 or 180 min. Data are represented as means ± S.D. for three experiments. Gefitinib concentration in the samples was determined with LC-MS/MS. ( b ) Bidirectional transport of imatinib. ABCB4 and Mock cells were incubated with 0.5 µM imatinib for 60, 120 or 180 min. Imatinib concentration in the samples was determined with LC-MS/MS. Data are represented as means ± S.D. from three independent experiments.

Article Snippet: An Abcb1 biallelic knockout MDCKII (Abcb1KO-MDCKII) cell clone was generated by GenScript (Leiden, the Netherlands) using the CRISPR/Cas9 system.

Techniques: Incubation, Concentration Assay, Liquid Chromatography with Mass Spectroscopy

Journal: ACS Omega

Article Title: Conducting Polymer Scaffolds Based on Poly(3,4-ethylenedioxythiophene) and Xanthan Gum for Live-Cell Monitoring

doi: 10.1021/acsomega.8b00458

Figure Lengend Snippet: MDCK II eGFP cell growth on PEDOT:xanthan gum (0.5:0.5) 2% solid content scaffold 14 days after culture, 37 °C, 5% CO 2 . (a) Confocal microscopy image of the entire scaffold 14 days after cell seeding and (b) zoomed image of the scaffold 14 days after cell seeding.

Article Snippet: MDCK II LifeAct cells were prepared for this study in the same manner according to the manufacturer guidelines (Ibidi, GmbH) and as described previously using pCMVLifeAct-TagRFP.

Techniques: Confocal Microscopy

Journal: ACS Omega

Article Title: Conducting Polymer Scaffolds Based on Poly(3,4-ethylenedioxythiophene) and Xanthan Gum for Live-Cell Monitoring

doi: 10.1021/acsomega.8b00458

Figure Lengend Snippet: Cell growth on PEDOT:xanthan gum (0.5:0.5) 2% solid content scaffolds and impedance measurements. (a) Device configuration of the scaffold-based electrode. (b) Confocal microscopy image of the entire scaffold 7 days after cell seeding with fluorescent MDCK II LifeAct cells and (c,d) zoomed images of the scaffold 7 days after cell seeding. Impedance spectra showing the (e) complex impedance data, (f) the phase angle vs frequency, and the (g) Nyquist plots of the scaffolds before and after cell culture for 7 days. The dashed lines represent the spectra of a PEDOT:xanthan gum scaffold after 7 days of incubation (37 °C, 5% CO 2 ) in culture media without cells.

Article Snippet: MDCK II LifeAct cells were prepared for this study in the same manner according to the manufacturer guidelines (Ibidi, GmbH) and as described previously using pCMVLifeAct-TagRFP.

Techniques: Confocal Microscopy, Cell Culture, Incubation