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Image Search Results
Journal: Aging (Albany NY)
Article Title: SAMP-Score: a morphology-based machine learning classification method for screening pro-senescence compounds in p16 positive cancer cells
doi: 10.18632/aging.206333
Figure Lengend Snippet: Validation of QM0005928 (QM5928). ( A ) QM0005928 chemical structure. ( B ) Heatmap representing high content analysis feature (HCA; y-axis) profiles of a dose response of QM5928 (x-axis). ( C ) Line plot showing change in SAMP score with increasing doses of QM5928. ( D ) Raw cell counts of increasing doses of QM5928 (Red = NonSen and Blue = Sen SAMP-Score Classification). ( E , F ) Z-scores for cell count and cell area increasing doses of QM5928. (Red = NonSen and Blue = Sen SAMP-Score Classification). ( G ) Western plot showing p16 expression in MB-468, BT549 and HeLa Sen-Mark+ cancer lines. ( H , I ) Cell counts for MB-468, BT549 and HeLa Sen-Mark+ cancer lines in response to QM5928. N = 3. Scale bar = 100 μm. ( J ) Heatmap representing high content analysis feature (HCA; y-axis) profiles of HeLa screen treatments that phenocopy QM5928 by hierarchical clustering. ( K ) KEGG pathways analysis of HeLa screen treatments that phenocopy QM5928 by hierarchical clustering. The data for MB-468s in and are derived from the same experimental dataset, presented in different formats to emphasise distinct aspects of the findings.
Article Snippet: HeLa (CRUK),
Techniques: Biomarker Discovery, High Content Screening, Cell Characterization, Western Blot, Expressing, Derivative Assay
Journal: Aging (Albany NY)
Article Title: SAMP-Score: a morphology-based machine learning classification method for screening pro-senescence compounds in p16 positive cancer cells
doi: 10.18632/aging.206333
Figure Lengend Snippet: Interplay between p16 localisation and QM5928. ( A ) Immunofluorescence staining of MB-468s treated with either DMSO or QM5928 for DAPI, p16 (Santa Cruz - FITC), p16 (Protein Tech - Cy3) and Cell Mask. Secondary only controls (2nd) did not receive primary p16 antibodies. ( B ) Digital zoom of white boxed areas in A, for p16 (Protein Tech - Cy3). Scale bars = 100 μm. ( C , D ) Scaled probability density estimate quantitation of nuclear/cytoplasmic intensity ratios for MB-468s stained with p16 in the FITC and Cy3 channels. A higher value indicates increased nuclear staining. Area under the curve is equal to 100% of the population. N = 3. ( E , F ) Cell counts for MB-468 (p16 positive - blue) and MB-231 (p16-null - red) cancer lines in response to QM5928 at day 6 or day 10. N = 3. The data for MB-468s in and are derived from the same experimental dataset, presented in different formats to emphasise distinct aspects of the findings.
Article Snippet: HeLa (CRUK),
Techniques: Immunofluorescence, Staining, Quantitation Assay, Derivative Assay