Journal: Cell Reports Medicine

Article Title: ACVR2A attenuation impacts lactate production and hyperglycolytic conditions attracting regulatory T cells in hepatocellular carcinoma

doi: 10.1016/j.xcrm.2025.102038

Figure Lengend Snippet: Impairment of Treg cell infiltration by MCT inhibition in ACVR2A -KO HCC (A) Western blot analysis of MCT4 expression in HuH7 KO and Hepa1-6 KO cells. β-Actin was used as an internal control. (B) Dose-response curves of the MCT4 inhibitor VB124 in HuH7 KO and Hepa1-6 KO cells. (C and D) Proliferation (C) and colony formation (D) assays of HuH7 KO and Hepa1-6 KO cells treated with VB124. Representative photo images in each assay were included. The p value was calculated by Welch’s t test (C). The p value was calculated by Mann-Whitney U test (D). (E and F) Quantitative flow cytometric analysis of Foxp-3 + Treg cells co-cultured with HuH7 KO cells (E) and Hepa1-6 KO cells (F). The p value was calculated using Mann-Whitney U test or Kruskal-Wallis test with Steel-Dwass post hoc test. Boxes represent the 25th, 50th, and 75th percentiles. Data are the mean ± SD. n.s, not significant; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: MCT4 Polyclonal Antibody , Proteintech , Cat# 36169S; RRID: AB_2799095.

Techniques: Inhibition, Western Blot, Expressing, Control, MANN-WHITNEY, Cell Culture

Journal: Cell Reports Medicine

Article Title: ACVR2A attenuation impacts lactate production and hyperglycolytic conditions attracting regulatory T cells in hepatocellular carcinoma

doi: 10.1016/j.xcrm.2025.102038

Figure Lengend Snippet: Synergistic anti-tumor effects of MCT inhibition and immune checkpoint blockade on ACVR2A -KO HCC (A) Tumorigenicity assay of Hepa1-6 KO cells with Mct4 knockdown in immunoproficient mice treated with anti-PD-1 antibody ( n = 4). Representative photo images of tumor specimens were included. The p value was calculated by ANOVA with Tukey-Kramer post hoc test. (B) Quantitative immunohistochemical analysis of CD8 + T cell and Foxp-3 + T cell infiltration. The p value was calculated using Kruskal-Wallis test with Steel-Dwass post hoc test. (C) CD8 + T cell/Foxp-3 + Treg cell ratio. The p value was calculated using Kruskal-Wallis test with Steel-Dwass post hoc test. (D) Intratumoral lactate levels. The p value was calculated using Kruskal-Wallis test with Steel-Dwass post hoc test. (E) Tumorigenicity assay of Hepa1-6 KO cells in immunoproficient mice treated with anti-PD-1 antibody and VB124 ( N = 4). Representative photo images of tumor specimens were included. The p value was calculated by ANOVA with Tukey-Kramer post hoc test. (F) Quantitative immunohistochemical analysis of CD8 + T cell and Foxp-3 + T cell infiltration. The p value was calculated using Kruskal-Wallis test with Steel-Dwass post hoc test. (G) CD8 + T cell/Foxp-3 + Treg cell ratio. The p value was calculated using Kruskal-Wallis test with Steel-Dwass post hoc test. (H) Intratumoral lactate levels. The p value was calculated using Kruskal-Wallis test with Steel-Dwass post hoc test. (I) Tumorigenicity assay of 3H3-Pten-KO cells with Acvr2a knockout in immunoproficient mice treated with anti-PD-1 antibody and VB124 ( N = 4). Representative photo images of tumor specimens were included. The p value was calculated by ANOVA with Tukey-Kramer post hoc test. (J) Quantitative immunohistochemical analysis of CD8 + T cell and Foxp-3 + T cell infiltration. The p value was calculated using Kruskal-Wallis test with Steel-Dwass post hoc test. (K) CD8 + T cell/Foxp-3 + Treg cell ratio. The p value was calculated using Kruskal-Wallis test with Steel-Dwass post hoc test. (L) Intratumoral lactate levels. The p value was calculated using Kruskal-Wallis test with Steel-Dwass post hoc test. (M and N) Quantitative flow cytometric analysis of Foxp-3 + Treg cells and CD8 + T cells with exhaustion and activation markers in Hepa1-6 KO (M) and 3H3-Pten-KO with Acvr2a -knockout (N) xenografts. The p value was calculated using Mann-Whitney U test. (O) Representative case of HCC with high SUVmax. The left and right panels show an FDG-PET/CT image and representative immunohistochemical images of ACVR2A, LDHA and Treg cells, respectively. Nuclei were stained using hematoxylin. The scale bar represents 100 or 200 μm. Boxes represent the 25th, 50th, and 75th percentiles. Data are the mean ± SD. n.s, not significant; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: MCT4 Polyclonal Antibody , Proteintech , Cat# 36169S; RRID: AB_2799095.

Techniques: Inhibition, Tumorigenicity Assay, Knockdown, Immunohistochemical staining, Knock-Out, Activation Assay, MANN-WHITNEY, Positron Emission Tomography-Computed Tomography, Staining

Journal: Cell Reports Medicine

Article Title: ACVR2A attenuation impacts lactate production and hyperglycolytic conditions attracting regulatory T cells in hepatocellular carcinoma

doi: 10.1016/j.xcrm.2025.102038

Figure Lengend Snippet:

Article Snippet: MCT4 Polyclonal Antibody , Proteintech , Cat# 36169S; RRID: AB_2799095.

Techniques: Staining, Purification, Recombinant, Modification, Transfection, Functional Assay, Antibody Labeling, Lactate Assay, Glucose Assay, Blocking Assay, Western Blot, Reverse Transcription, Cell Culture, Bicinchoninic Acid Protein Assay, RNA Sequencing, Gene Expression, Plasmid Preparation, shRNA, Software, Real-time Polymerase Chain Reaction, Filtration

Journal: Cell Reports Medicine

Article Title: ACVR2A attenuation impacts lactate production and hyperglycolytic conditions attracting regulatory T cells in hepatocellular carcinoma

doi: 10.1016/j.xcrm.2025.102038

Figure Lengend Snippet:

Article Snippet: PGI 20139-1-AP MCT1 Polyclonal antibody , Proteintech , Cat# 20139-1-AP; RRID: AB_2878645.

Techniques: Staining, Purification, Recombinant, Modification, Transfection, Functional Assay, Antibody Labeling, Lactate Assay, Glucose Assay, Blocking Assay, Western Blot, Reverse Transcription, Cell Culture, Bicinchoninic Acid Protein Assay, RNA Sequencing, Gene Expression, Plasmid Preparation, shRNA, Software, Real-time Polymerase Chain Reaction, Filtration