mcmbp Search Results


nbp1 90746  (Novus Biologicals)
90
Novus Biologicals nbp1 90746
Nbp1 90746, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nbp1 90746/product/Novus Biologicals
Average 90 stars, based on 1 article reviews
nbp1 90746 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
gfp  (Proteintech)
93
Proteintech gfp
Gfp, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfp/product/Proteintech
Average 93 stars, based on 1 article reviews
gfp - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
rabbit polyclonal anti mcmbp  (Atlas Antibodies)
93
Atlas Antibodies rabbit polyclonal anti mcmbp

Rabbit Polyclonal Anti Mcmbp, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti mcmbp/product/Atlas Antibodies
Average 93 stars, based on 1 article reviews
rabbit polyclonal anti mcmbp - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
mcmbp  (Novus Biologicals)
93
Novus Biologicals mcmbp
a, A schematic describing the experimental approach for understanding the role of CUL4-DCAF12 <t>in</t> <t>MCM2-7</t> assembly (see text for details). b, Halo-immunoprecipitation (Halo IP) of whole cell lysates followed by western blotting, cells were treated with siRNA against DCAF12 (siDCAF12) as indicated. This IP was done at low salt concentration (150 mM NaCl) c, Quantification of Halo-IP products based on western blots in b ; IP products upon control siRNA treatments have been considered as 100% (data are mean ± s.d.; n = 2 biological replicates). d, Halo IP of whole cell lysates followed by western blotting, cells were treated with siRNA against DCAF12 (siDCAF12) as indicated. This IP was done at a high salt concentration (500 mM NaCl). e, Quantification of Halo-IP products based on western blots in d ; IP products upon control siRNA treatments have been considered as 100% (data mean ± s.d.; n = 2 biological replicates). e, Heatmap, derived from mass spectrometry analysis of HEK293T cells co- transfected with StrepII-FLAG-tagged DCAF12, shows log2 intensities of indicated proteins. Cells were treated with either DMSO (CTRL) or 10 µM MG132 for 6 hours prior to protein purification. g, Schematic representation of human DCAF12 and its N-terminally truncated mutants. Two predicted nuclear localization signals (NLS) and H-box are highlighted. h, i, QIBC plots of the cytoplasm of U2OS cells stained for StrepII-tagged DCAF12 ( h ) or <t>MCMBP</t> ( i ) protein after indicated DCAF12 constructs were inducibly expressed. Lines denote medians; n ≈ 1,000 cells per condition.
Mcmbp, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mcmbp/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
mcmbp - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
mcmbp fl/fl mice  (GemPharmatech Co Ltd)
90
GemPharmatech Co Ltd mcmbp fl/fl mice
a, A schematic describing the experimental approach for understanding the role of CUL4-DCAF12 <t>in</t> <t>MCM2-7</t> assembly (see text for details). b, Halo-immunoprecipitation (Halo IP) of whole cell lysates followed by western blotting, cells were treated with siRNA against DCAF12 (siDCAF12) as indicated. This IP was done at low salt concentration (150 mM NaCl) c, Quantification of Halo-IP products based on western blots in b ; IP products upon control siRNA treatments have been considered as 100% (data are mean ± s.d.; n = 2 biological replicates). d, Halo IP of whole cell lysates followed by western blotting, cells were treated with siRNA against DCAF12 (siDCAF12) as indicated. This IP was done at a high salt concentration (500 mM NaCl). e, Quantification of Halo-IP products based on western blots in d ; IP products upon control siRNA treatments have been considered as 100% (data mean ± s.d.; n = 2 biological replicates). e, Heatmap, derived from mass spectrometry analysis of HEK293T cells co- transfected with StrepII-FLAG-tagged DCAF12, shows log2 intensities of indicated proteins. Cells were treated with either DMSO (CTRL) or 10 µM MG132 for 6 hours prior to protein purification. g, Schematic representation of human DCAF12 and its N-terminally truncated mutants. Two predicted nuclear localization signals (NLS) and H-box are highlighted. h, i, QIBC plots of the cytoplasm of U2OS cells stained for StrepII-tagged DCAF12 ( h ) or <t>MCMBP</t> ( i ) protein after indicated DCAF12 constructs were inducibly expressed. Lines denote medians; n ≈ 1,000 cells per condition.
Mcmbp Fl/Fl Mice, supplied by GemPharmatech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mcmbp fl/fl mice/product/GemPharmatech Co Ltd
Average 90 stars, based on 1 article reviews
mcmbp fl/fl mice - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


Journal: iScience

Article Title: Bromodomain protein BRD8 regulates cell cycle progression in colorectal cancer cells through a TIP60-independent regulation of the pre-RC complex

doi: 10.1016/j.isci.2023.106563

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-MCMBP , Atlas Antibodies , Cat# HPA038481; RRID:AB_10794620.

Techniques: Recombinant, Plasmid Preparation, Cell Cycle Assay, CCK-8 Assay, Flow Cytometry, Viability Assay, Ubiquitin Proteomics, Expressing, Cloning, Mutagenesis, Software

a, A schematic describing the experimental approach for understanding the role of CUL4-DCAF12 in MCM2-7 assembly (see text for details). b, Halo-immunoprecipitation (Halo IP) of whole cell lysates followed by western blotting, cells were treated with siRNA against DCAF12 (siDCAF12) as indicated. This IP was done at low salt concentration (150 mM NaCl) c, Quantification of Halo-IP products based on western blots in b ; IP products upon control siRNA treatments have been considered as 100% (data are mean ± s.d.; n = 2 biological replicates). d, Halo IP of whole cell lysates followed by western blotting, cells were treated with siRNA against DCAF12 (siDCAF12) as indicated. This IP was done at a high salt concentration (500 mM NaCl). e, Quantification of Halo-IP products based on western blots in d ; IP products upon control siRNA treatments have been considered as 100% (data mean ± s.d.; n = 2 biological replicates). e, Heatmap, derived from mass spectrometry analysis of HEK293T cells co- transfected with StrepII-FLAG-tagged DCAF12, shows log2 intensities of indicated proteins. Cells were treated with either DMSO (CTRL) or 10 µM MG132 for 6 hours prior to protein purification. g, Schematic representation of human DCAF12 and its N-terminally truncated mutants. Two predicted nuclear localization signals (NLS) and H-box are highlighted. h, i, QIBC plots of the cytoplasm of U2OS cells stained for StrepII-tagged DCAF12 ( h ) or MCMBP ( i ) protein after indicated DCAF12 constructs were inducibly expressed. Lines denote medians; n ≈ 1,000 cells per condition.

Journal: bioRxiv

Article Title: CRL4 DCAF12 regulation of MCMBP ensures optimal licensing of DNA replication

doi: 10.1101/2024.11.26.625391

Figure Lengend Snippet: a, A schematic describing the experimental approach for understanding the role of CUL4-DCAF12 in MCM2-7 assembly (see text for details). b, Halo-immunoprecipitation (Halo IP) of whole cell lysates followed by western blotting, cells were treated with siRNA against DCAF12 (siDCAF12) as indicated. This IP was done at low salt concentration (150 mM NaCl) c, Quantification of Halo-IP products based on western blots in b ; IP products upon control siRNA treatments have been considered as 100% (data are mean ± s.d.; n = 2 biological replicates). d, Halo IP of whole cell lysates followed by western blotting, cells were treated with siRNA against DCAF12 (siDCAF12) as indicated. This IP was done at a high salt concentration (500 mM NaCl). e, Quantification of Halo-IP products based on western blots in d ; IP products upon control siRNA treatments have been considered as 100% (data mean ± s.d.; n = 2 biological replicates). e, Heatmap, derived from mass spectrometry analysis of HEK293T cells co- transfected with StrepII-FLAG-tagged DCAF12, shows log2 intensities of indicated proteins. Cells were treated with either DMSO (CTRL) or 10 µM MG132 for 6 hours prior to protein purification. g, Schematic representation of human DCAF12 and its N-terminally truncated mutants. Two predicted nuclear localization signals (NLS) and H-box are highlighted. h, i, QIBC plots of the cytoplasm of U2OS cells stained for StrepII-tagged DCAF12 ( h ) or MCMBP ( i ) protein after indicated DCAF12 constructs were inducibly expressed. Lines denote medians; n ≈ 1,000 cells per condition.

Article Snippet: Primary antibodies used for immunofluorescence (IF) were as follows: CDT1 (rabbit, Abcam, ab202067, 1:2,000), Cyclin D1 (rabbit, Proteintech, 26939-1-AP, 1:1,000), GFP (rabbit, Proteintech, PABG1, 1:5,000), MCMBP (rabbit, Novus Biologicals, NBP1-90746, 1:1,000), MCM2 (rabbit, Proteintech, 10513-1-AP, 1:1,000), MCM3 (mouse, Santa Cruz, sc-390480, 1:1,000), MCM4 (rabbit, Proteintech, 13043-1-AP, 1:1,000), MCM5 (rabbit, Proteintech, 11703-1-AP, 1:1,000), MCM6 (mouse, Novus Biologicals, H00004175-M04, 1:1,000), MCM7 (mouse, Santa Cruz, sc-9966, 1:1,000), PCNA (human, Immuno Concepts, 2037, 1:1,000), RAD51 (rabbit, BioAcademia, 70-012, 1:1,000), Strep II Tag (mouse, Novus Biologicals, NBP2-43735, 1:1,000), γH2AX (Ser139) (rabbit, Abcam, ab81299, 1:1,000).

Techniques: Immunoprecipitation, Western Blot, Concentration Assay, Control, Derivative Assay, Mass Spectrometry, Transfection, Protein Purification, Staining, Construct