Journal: International Journal of Molecular Medicine

Article Title: Novel effect of estrogen on RANK and c-fms expression in RAW 264.7 cells

doi: 10.3892/ijmm.20.1.97

Figure Lengend Snippet: Figure 1. (Left panels) Estrogen receptors ER α and ß RNA expression in macrophage cells. RAW 264.7 cells were analyzed by RT-PCR for the presence of the mRNA encoding ER α and ß. Cells were cultured under standard growth conditions, and RNA was extracted and retrotranscribed using the reverse transcriptase enzyme. ER α and ß were expressed as specific single bands. (Right panels) Western blot analysis for ERs. The whole cell lysates from RAW 264.7 cells were analyzed for the expression of ER α and ß. The positive controls were MCF7 whole cell lysate for α and NIH/3T3 whole cell lysate for ß. Cont, control.

Article Snippet: Controls for both α and ß were used (MCF7 whole cell lysate for α and NIH/3T3 whole cell lysate for ß, Santa Cruz Biotechnology, Inc., CA).

Techniques: RNA Expression, Reverse Transcription Polymerase Chain Reaction, Cell Culture, Reverse Transcription, Western Blot, Expressing, Control

Journal: ACS Omega

Article Title: Mechanical Transfer of Black Phosphorus on a Silk Fibroin Substrate: A Viable Method for Photoresponsive and Printable Biomaterials

doi: 10.1021/acsomega.3c09461

Figure Lengend Snippet: (a) Viability of MCF7 cancer cells after 24 h of incubation with PBS 1× (CTR), SF, SF/BP 0.03 mg, SF/BP 0.056 mg, and BP 0.056 mg reported without irradiation (in gray), irradiation with a 110 kJ/m 2 dose for 220 s (in blue), and irradiation with a 220 kJ/m 2 dose for 440 s (in orange). (b) AO–DAPI double staining was performed for each condition, and viability (%) was reported in each image.

Article Snippet: MCF7 cells were used as a well-characterized breast cancer cell line and were purchased from Elabscience Biotechnology (Houston, Texas).

Techniques: Incubation, Irradiation, Double Staining

Journal: Oncotarget

Article Title: The TGFα-EGFR-Akt signaling axis plays a role in enhancing proinflammatory chemokines in triple-negative breast cancer cells

doi: 10.18632/oncotarget.25389

Figure Lengend Snippet: ( A ) Heatmap for RNA expression levels of chemokines in representative human TNBC (BT549, MB231 and MB468) and non-TNBC (T47D, MCF7) cell lines. ( B ) Heatmap for RNA expression levels of chemokine receptors in TNBC and non-TNBC cell lines. After isolating total RNA and choosing the qualified RNAs, a human chemokine PCR array was performed. Red trend indicates high expression levels of chemokines. ( C ) The status of estrogen receptor (ER), progesterone receptor (PR) and HER2 expression in TNBC and LA-BC cells. ( D ) Average intensity for the expression levels of dominant chemokines and chemokine receptors in TNBC and LA-BC cells. Red and blue bars indicate TNBC and LA-BC cells, respectively.

Article Snippet: The PIK3CA activity in BT549 and MCF7 cells (lysate) after time-dependent treatment with EGF and TNF was analyzed by an ELISA kit (Aviva Systems Biology Corporation, San Diego, CA, USA) according to the manufacturer's instructions.

Techniques: RNA Expression, Expressing

Journal: Oncotarget

Article Title: The TGFα-EGFR-Akt signaling axis plays a role in enhancing proinflammatory chemokines in triple-negative breast cancer cells

doi: 10.18632/oncotarget.25389

Figure Lengend Snippet: ( A ) Heatmap for RNA expression levels of EGFR family members in human BC tissues from TCGA-based dataset using Gitools 2.3.1. ( B ) Statistical analysis for RNA expression levels of EGFR family members in human BC tissues. The red, yellow, blue and green colors indicate BL, HER2, LA and LB samples, respectively. The asterisk ( * ) and hash (#) indicate a statistically significant increase and decrease ( p ≤ 0.05) as calculated by ANOVA and Tukey's pairwise comparisons, respectively. ( C ) Heatmap for RNA expression levels of EGFR family members based on analysis of the GEO dataset (Accession: GSE12777) for 51 human BC cell lines using Gitools 2.3.1. Pink, yellow and green dots indicate high expression levels in BL-TNBC, HER2-BC and LB-BC cells, respectively. ( D ) Protein levels of EGFR family members in representative TNBC (MB468, MB231, and BT549) and non-TNBC (MCF7 and T47D) cells. β-actin was used as the loading control.

Article Snippet: The PIK3CA activity in BT549 and MCF7 cells (lysate) after time-dependent treatment with EGF and TNF was analyzed by an ELISA kit (Aviva Systems Biology Corporation, San Diego, CA, USA) according to the manufacturer's instructions.

Techniques: RNA Expression, Expressing, Control

Journal: Oncotarget

Article Title: The TGFα-EGFR-Akt signaling axis plays a role in enhancing proinflammatory chemokines in triple-negative breast cancer cells

doi: 10.18632/oncotarget.25389

Figure Lengend Snippet: Chemokine signatures in non-TNBC (MCF7) and TNBC (BT549) cells after a 1-h stimulation with recombinant human EGF (10 ng/ml) as revealed by a human chemokine PCR array. Chemokines with duplicate average cycle threshold of <30 are considered dominant. The asterisk ( * ) and hash ( # ) indicate increases and decreases that are larger than two-fold, respectively.

Article Snippet: The PIK3CA activity in BT549 and MCF7 cells (lysate) after time-dependent treatment with EGF and TNF was analyzed by an ELISA kit (Aviva Systems Biology Corporation, San Diego, CA, USA) according to the manufacturer's instructions.

Techniques: Recombinant

Journal: Oncotarget

Article Title: The TGFα-EGFR-Akt signaling axis plays a role in enhancing proinflammatory chemokines in triple-negative breast cancer cells

doi: 10.18632/oncotarget.25389

Figure Lengend Snippet: ( A ) Basal protein levels of Akt and Erk in TNBC (BT549, MB231, MB468) and non-TNBC (MCF, T47D) cell lines. ( B ) Basal protein levels for NF-κB family members p65 (Rel A), Rel B, NF-κB (100/52), and NF-κB (105/50) in TNBC and non-TNBC cells. Human SKOV-3 and OVCAR-3 ovarian cancer cells were used as positive controls for the expression of NF-κB signaling components. ( C ) Protein expression profiles of IκB, Akt, Erk and their phosphorylated forms in BT549 and MCF7 cells in response to EGF (10 ng/ml) and TNF (10 ng/ml) treatments. β-actin was used as the loading control.

Article Snippet: The PIK3CA activity in BT549 and MCF7 cells (lysate) after time-dependent treatment with EGF and TNF was analyzed by an ELISA kit (Aviva Systems Biology Corporation, San Diego, CA, USA) according to the manufacturer's instructions.

Techniques: Expressing, Control