Journal: International Journal of Molecular Medicine

Article Title: Novel effect of estrogen on RANK and c-fms expression in RAW 264.7 cells

doi: 10.3892/ijmm.20.1.97

Figure Lengend Snippet: Figure 1. (Left panels) Estrogen receptors ER α and ß RNA expression in macrophage cells. RAW 264.7 cells were analyzed by RT-PCR for the presence of the mRNA encoding ER α and ß. Cells were cultured under standard growth conditions, and RNA was extracted and retrotranscribed using the reverse transcriptase enzyme. ER α and ß were expressed as specific single bands. (Right panels) Western blot analysis for ERs. The whole cell lysates from RAW 264.7 cells were analyzed for the expression of ER α and ß. The positive controls were MCF7 whole cell lysate for α and NIH/3T3 whole cell lysate for ß. Cont, control.

Article Snippet: Controls for both α and ß were used (MCF7 whole cell lysate for α and NIH/3T3 whole cell lysate for ß, Santa Cruz Biotechnology, Inc., CA).

Techniques: RNA Expression, Reverse Transcription Polymerase Chain Reaction, Cell Culture, Reverse Transcription, Western Blot, Expressing, Control

Journal: ACS Omega

Article Title: Mechanical Transfer of Black Phosphorus on a Silk Fibroin Substrate: A Viable Method for Photoresponsive and Printable Biomaterials

doi: 10.1021/acsomega.3c09461

Figure Lengend Snippet: (a) Viability of MCF7 cancer cells after 24 h of incubation with PBS 1× (CTR), SF, SF/BP 0.03 mg, SF/BP 0.056 mg, and BP 0.056 mg reported without irradiation (in gray), irradiation with a 110 kJ/m 2 dose for 220 s (in blue), and irradiation with a 220 kJ/m 2 dose for 440 s (in orange). (b) AO–DAPI double staining was performed for each condition, and viability (%) was reported in each image.

Article Snippet: MCF7 cells were used as a well-characterized breast cancer cell line and were purchased from Elabscience Biotechnology (Houston, Texas).

Techniques: Incubation, Irradiation, Double Staining