Journal: Biomatter

Article Title: Cell infiltration into a 3D electrospun fiber and hydrogel hybrid scaffold implanted in the brain

doi: 10.1080/21592535.2015.1005527

Figure Lengend Snippet: Implant geometry 60 d post implantation. The tissue was sectioned transverse to the injection tract, displaying the cross sectional area of each implant, and stained for astrocytes (GFAP, red), macrophages/microglia (ED1, green), and nuclei (DAPI, blue). The samples were as follows: ( A ) Blank hydrogel, ( B ) PLLA fibers – low density, ( C ) PLLA + fibronectin fibers – low density, ( D ) PLLA fibers – high density, and ( E ) PLLA + fibronectin – high density. The cross sectional area for each implant was not significantly altered as a function of time or scaffold composition. Demarcated areas indicate the regions captured at high magnification for . Images were captured with a 10X objective lens using the slide scan function in MetaMorph. Z-stack ranges were optimized for each fluorescence channel and captured independently. Scale bars represent 250 μm.

Article Snippet: Each slide was then washed with PBS and immersed in antibody diluent (0.2% NGS and 0.01% Triton X100) with rabbit anti-rat glial fibrillary acid protein (Astrocytes; GFAP, Dako, Z0334), mouse anti-rat ED1 (Macrophage/Microglia; AbDSerotec, MCA314R), or anti-neurofilament (Neurons; Developmental Studies Hybridoma Bank, RT97) all diluted at 1:500 and incubated overnight at room temperature.

Techniques: Injection, Staining, Fluorescence

Journal: Biomatter

Article Title: Cell infiltration into a 3D electrospun fiber and hydrogel hybrid scaffold implanted in the brain

doi: 10.1080/21592535.2015.1005527

Figure Lengend Snippet: Astrocyte and macrophage/microglia interactions with hybrid scaffolds implanted into the striatum 60 d post implantation. High magnification images (demarcated areas of corresponding images in ) of astrocytes (GFAP, red) and macrophages/microglia (ED1, green) at the tissue-scaffold interface display cellular infiltration into the hybrid scaffold and utilization of the electrospun fiber network. Nuclei were labeled with DAPI (blue). The samples were as follows: ( A ) Blank hydrogel, ( B ) PLLA fibers – low density, ( C ) PLLA + fibronectin fibers – low density, ( D ) PLLA fibers – high density, and ( E ) PLLA + fibronectin – high density. The fibronectin fiber samples, ( C and E ), display a greater extent of cellular infiltration into the hybrid matrix as well as a more loosely defined glial boundary as compared to that displayed in B. Images were captured with a 40X objective lens. Z-stack ranges were optimized for each fluorescence channel and captured independently. Scale bar represents 50 μm.

Article Snippet: Each slide was then washed with PBS and immersed in antibody diluent (0.2% NGS and 0.01% Triton X100) with rabbit anti-rat glial fibrillary acid protein (Astrocytes; GFAP, Dako, Z0334), mouse anti-rat ED1 (Macrophage/Microglia; AbDSerotec, MCA314R), or anti-neurofilament (Neurons; Developmental Studies Hybridoma Bank, RT97) all diluted at 1:500 and incubated overnight at room temperature.

Techniques: Labeling, Fluorescence