Journal: Journal of Neuroscience

Article Title: The Protein Kinase A Regulatory Subunit R1A (Prkar1a) Plays Critical Roles in Peripheral Nerve Development

doi: 10.1523/jneurosci.0766-13.2013

Figure Lengend Snippet: Figure4. TheonsetofmyelinationisearlyinPrkar1a-SCKOsciaticnerves.A,EMshows1:1SC-axonrelations(asterisks)incontrolandPrkar1a-SCKOsciaticnervesatE18.Bottom,moremyelin wraps were present in Prkar1a-SCKO sciatic nerves. B, Oct6 expression was increased in Prkar1a-SCKO sciatic nerves stained with anti-Oct6. C, MBP expression was increased in E18 Prkar1a-SCKO sciatic nerves. D, EM shows myelinated axons (asterisks) in Prkar1a-SCKO sciatic nerves at P0. There was a significant increase in myelinated axons in E18–P0 Prkar1a-SCKO nerves. For each genotype,n6miceatE18–P0(***p0.001,Student’sttest).Errorbarsindicate SEM.E,MBPandPmp22expressionwereincreased,whereasKrox20wasslightlydecreasedinPrkar1a-SCKO sciatic nerve lysates. Total ErbB3, P-Akt, and P-Erk1/2 were decreased in Prkar1a-SCKO sciatic nerve lysates by Western blot (n 3). -Actin was used as a loading control. Scale bars: A, 1 M; B, C, 25 M; D, 2 M.

Article Snippet: The following primary antibodies were used: Ki67 (Ventana); MBP (Aves Labs); Oct-6 (Santa Cruz Biotechnology); neurofilament (Developmen- tal Studies Hybridoma Bank).

Techniques: Expressing, Staining, Western Blot, Control

Journal: bioRxiv

Article Title: Curcumin Promotes Myelin Repair after Spinal Cord Injury via Spatially Selective Regulation of CLASP2 Phosphorylation

doi: 10.64898/2026.01.21.700776

Figure Lengend Snippet: (A) Schematic representation of study design. In thorax vertebrae 8-9 region of mice, 1 mm thick of spinal cord tissue was excised, and penicillin was injected muscularly daily for 3 days post-surgery. The groups were assigned to receive PBS, with the middle-dose group receiving 200 mg/kg/day and the high-dose group receiving 400 mg/kg/day. (B) BMS scores in mice rear legs of diverse groups. Values = means ± SEM. One-way analysis of variance (ANOVA) was conducted to examine the differences between groups, n=3 for each group. Con vs. CM-M, # p <0.05, ## p <0.01; Con vs. CM-H, * p <0.05, ** p <0.01, *** p <0.001. (C) Representative pictures of the hindlimb gait of control (complete spinal cord transection injury group), and treat (medium-dose group and high-dose group of curcumin). (D) Probability distribution chart of ankle dorsifiexion angles in each group. (E) Fibrotic scar and glial scar in the injured area of different groups. Immunostaining of tissues with chicken anti-GFAP (green) antibodies and mouse anti-Laminin antibodies (red). Nuclei were stained with DAPI. Scale bar represent 5 μm. (F) The myelin sheath and glial scar in the injured area of different groups. Immunostaining of tissues with chicken anti-GFAP (green) antibodies and mouse anti-MBP antibodies (red). Nuclei were stained with DAPI. Scale bar represent 5 μm. (G) Area of the fibrous scar defined by the fibrous scar marker Laminin from (E). Values = means ± SEM. One-way ANOVA, n=3 for each group. * P <0.05, ** P <0.01, n.s P> 0.05. (H) Area of the glial scar defined by the glial scar marker GFAP from (E). Values = means ± SEM. One-way ANOVA, n=3 for each group. n.s P> 0.05. (I) Integrated density of myelin sheath defined by MBP from (F). Values = means ± SEM. One-way ANOVA, n=3 for each group. * P <0.05, n.s P> 0.05.

Article Snippet: Avertin (Sigma-Aldrich, T48402-25G), penicillin sodium (FeiyuBIO, FY20311), Curcumin (Solarbio, C7090), NaOH (SCR, 1310-73-2), Na 2 HPO 4 (SCR, 7558-79-4), NaH 2 PO 4 (SCR, 13472-35-0), NaCl (SCR, 7647-14-5), Paraformaldehyde (SCR, 30525-89-4), Sucrose (SCR, 57-50-1), Triton-X-100 (Aladdin, T109026), O.C.T compound (Sakura, 4583), Tris (Solarbio, T8060), HCl (SCR, 7647-01-0), Ethylene Diamine Tetraacetic Acid (SCR, 60-00-4), NP-40 (Thermo Scientific, 28324), Na 3 VO 4 (Sigma-Aldrich, S6508-10G), NaF (SCR, 7681-49-4), Na 4 P 2 O 7 (Sigma-Aldrich, 7722-88-5), β-glycerophosphate(Sigma-Aldrich, 154804-51-0), Phosphatase inhibitor cocktail (Sigma-Aldrich, 524633), Complete Protease Inhibitor Cocktail (Roche, CO-RO), sodium deoxycholate (Sigma-Aldrich, V900388-50G), acetic acid (SCR, 64-19-7), sodium acetate (SCR, 127-09-3), zinc acetate (SCR, 5970-45-6), Phos-tag Agarose (Wako, 302-93561), L-Glutathione reduced (Sigma-Aldrich, 70-18-8), Nonfat dry milk (Beyotime, P0216), ECL kit (PerkinElmer, 122799-10), Tween-20 (Solarbio, T8220), TEMED (Amresco, 110-18-9), 30% Acrylamide (Solarbio, A1010), ammonium persulfate (SCR, 7727-54-0), methyl alcohol(SCR, 67-56-1), Fetal bovine serum (VivaCell, C04001-050), P/S (Pricella, PB180120), Bovine serum albumin (AMRESCO, 9048-46-8), Typsin (Sigma-Aldrich, R00950), Poly-L-lysine (Yeasen, 60716ES08), AntiFade Reagent (Sigma-Aldrich, P36930-2), FluorSave Reagent (Millipore, 345789), Laminin (Abcam, IF 1:1000, ab133645), MBP (SANTA CRUZ, IF 1:100, sc-66064), GFAP (Abcam, IF 1:1000, ab133645 ab7260), NFH (Abcam, IF 1:1000, ab207176), EB1 (BD, IF 1:500, 610535), GM130 (BD, IF 1:500, 610822), Clasp2 (CST, WB 1:1000, 14629s), acetylated-tubulin (Sigma-Aldrich, IF 1:6000, WB 1:10000 T6793), GFP (Sigma-Aldrich, IF 1:1000, G1546), DAPI (Sigma-Aldrich, 28718-90-3), anti-α-tubulin antibody (Sigma-Aldrich, T9026, 1:10000), goat HRP-conjugated anti-mouse IgG (CWBIO, CW0102S, WB 1:1000), goat Alexa Fluor 555-conjugated anti-rabbit (Invitrogen, IF 1:500, A27039), goat Alexa Fluor 488-conjugated anti-mouse (Invitrogen, IF 1:500, A11001), goat Alexa Fluor 488-conjugated anti-chicken (Invitrogen, IF 1:500, A11039).

Techniques: Injection, Control, Immunostaining, Staining, Marker