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  • 97
    Millipore mbp
    Together with CPP1, DAY regulates the stability of protochlorophyllide oxidoreductase (POR) through its holdase chaperone activity. a Reduction of DAY causes POR instability in N. benthamiana . Virus-induced gene silencing (VIGS) of N. benthamiana DAY ( NbDAY ) was performed. Then the VIGS plants (TRV:NbDAY) were agroinfiltrated with the POR C-GFP construct, and leaf mesophyll cells were examined by CLSM. POR C is a major POR isoform in light-grown Arabidopsis. NbDAY silencing caused a significant reduction in chlorophyll autofluorescence and POR C-GFP fluorescence. Scale bar, 20 µm. b DAY has holdase activity. Absorbance at 340 nm ( A 340 ) was measured at 1 min intervals to quantify turbidity. Thermal aggregation of malate dehydrogenase (2 µM) was measured at 43 °C over 16 min with 0–6 µM of <t>MBP-∆cTP</t> DAY or, as controls, 4 µM MBP-∆cTP/∆JL DAY (del JL) and 10 µM BSA. ∆cTP, deletion of cTP; ∆JL, deletion of the DnaJ- like domain (JL). c , d DAY interacts with POR C and CPP1 in chloroplasts. BiFC assays between DAY and POR C ( c ) or CPP1 ( d ). YFP signals were examined by CLSM in protoplasts after agroinfiltration. MAGNESIUM-PROTOPORPHYRIN IX METHYL TRANSFERASE (MgPMT), which is involved in chlorophyll biosynthesis, was used as a negative control. Scale bar, 10 µm ( c ) or 20 µm ( d ). e DAY directly interacts with CPP1 in vitro. Lysates of E. coli cells expressing <t>GST</t> or GST-CPP1 were incubated with MBP or MBP-∆cTP DAY (MBP-∆cTP) immobilized on amylose agarose resins. The resulting eluates were analyzed by immunoblotting using anti-GST antibody. The amount of MBP and MBP-∆cTP used in the pull-down assay were visualized by Coomassie staining.
    Mbp, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mbp/product/Millipore
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    86
    Millipore myelin basic protein mbp
    Together with CPP1, DAY regulates the stability of protochlorophyllide oxidoreductase (POR) through its holdase chaperone activity. a Reduction of DAY causes POR instability in N. benthamiana . Virus-induced gene silencing (VIGS) of N. benthamiana DAY ( NbDAY ) was performed. Then the VIGS plants (TRV:NbDAY) were agroinfiltrated with the POR C-GFP construct, and leaf mesophyll cells were examined by CLSM. POR C is a major POR isoform in light-grown Arabidopsis. NbDAY silencing caused a significant reduction in chlorophyll autofluorescence and POR C-GFP fluorescence. Scale bar, 20 µm. b DAY has holdase activity. Absorbance at 340 nm ( A 340 ) was measured at 1 min intervals to quantify turbidity. Thermal aggregation of malate dehydrogenase (2 µM) was measured at 43 °C over 16 min with 0–6 µM of <t>MBP-∆cTP</t> DAY or, as controls, 4 µM MBP-∆cTP/∆JL DAY (del JL) and 10 µM BSA. ∆cTP, deletion of cTP; ∆JL, deletion of the DnaJ- like domain (JL). c , d DAY interacts with POR C and CPP1 in chloroplasts. BiFC assays between DAY and POR C ( c ) or CPP1 ( d ). YFP signals were examined by CLSM in protoplasts after agroinfiltration. MAGNESIUM-PROTOPORPHYRIN IX METHYL TRANSFERASE (MgPMT), which is involved in chlorophyll biosynthesis, was used as a negative control. Scale bar, 10 µm ( c ) or 20 µm ( d ). e DAY directly interacts with CPP1 in vitro. Lysates of E. coli cells expressing <t>GST</t> or GST-CPP1 were incubated with MBP or MBP-∆cTP DAY (MBP-∆cTP) immobilized on amylose agarose resins. The resulting eluates were analyzed by immunoblotting using anti-GST antibody. The amount of MBP and MBP-∆cTP used in the pull-down assay were visualized by Coomassie staining.
    Myelin Basic Protein Mbp, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/myelin basic protein mbp/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    myelin basic protein mbp - by Bioz Stars, 2021-09
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    94
    New England Biolabs anti mbp antibody
    Together with CPP1, DAY regulates the stability of protochlorophyllide oxidoreductase (POR) through its holdase chaperone activity. a Reduction of DAY causes POR instability in N. benthamiana . Virus-induced gene silencing (VIGS) of N. benthamiana DAY ( NbDAY ) was performed. Then the VIGS plants (TRV:NbDAY) were agroinfiltrated with the POR C-GFP construct, and leaf mesophyll cells were examined by CLSM. POR C is a major POR isoform in light-grown Arabidopsis. NbDAY silencing caused a significant reduction in chlorophyll autofluorescence and POR C-GFP fluorescence. Scale bar, 20 µm. b DAY has holdase activity. Absorbance at 340 nm ( A 340 ) was measured at 1 min intervals to quantify turbidity. Thermal aggregation of malate dehydrogenase (2 µM) was measured at 43 °C over 16 min with 0–6 µM of <t>MBP-∆cTP</t> DAY or, as controls, 4 µM MBP-∆cTP/∆JL DAY (del JL) and 10 µM BSA. ∆cTP, deletion of cTP; ∆JL, deletion of the DnaJ- like domain (JL). c , d DAY interacts with POR C and CPP1 in chloroplasts. BiFC assays between DAY and POR C ( c ) or CPP1 ( d ). YFP signals were examined by CLSM in protoplasts after agroinfiltration. MAGNESIUM-PROTOPORPHYRIN IX METHYL TRANSFERASE (MgPMT), which is involved in chlorophyll biosynthesis, was used as a negative control. Scale bar, 10 µm ( c ) or 20 µm ( d ). e DAY directly interacts with CPP1 in vitro. Lysates of E. coli cells expressing <t>GST</t> or GST-CPP1 were incubated with MBP or MBP-∆cTP DAY (MBP-∆cTP) immobilized on amylose agarose resins. The resulting eluates were analyzed by immunoblotting using anti-GST antibody. The amount of MBP and MBP-∆cTP used in the pull-down assay were visualized by Coomassie staining.
    Anti Mbp Antibody, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mbp antibody/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mbp antibody - by Bioz Stars, 2021-09
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    Image Search Results


    Together with CPP1, DAY regulates the stability of protochlorophyllide oxidoreductase (POR) through its holdase chaperone activity. a Reduction of DAY causes POR instability in N. benthamiana . Virus-induced gene silencing (VIGS) of N. benthamiana DAY ( NbDAY ) was performed. Then the VIGS plants (TRV:NbDAY) were agroinfiltrated with the POR C-GFP construct, and leaf mesophyll cells were examined by CLSM. POR C is a major POR isoform in light-grown Arabidopsis. NbDAY silencing caused a significant reduction in chlorophyll autofluorescence and POR C-GFP fluorescence. Scale bar, 20 µm. b DAY has holdase activity. Absorbance at 340 nm ( A 340 ) was measured at 1 min intervals to quantify turbidity. Thermal aggregation of malate dehydrogenase (2 µM) was measured at 43 °C over 16 min with 0–6 µM of MBP-∆cTP DAY or, as controls, 4 µM MBP-∆cTP/∆JL DAY (del JL) and 10 µM BSA. ∆cTP, deletion of cTP; ∆JL, deletion of the DnaJ- like domain (JL). c , d DAY interacts with POR C and CPP1 in chloroplasts. BiFC assays between DAY and POR C ( c ) or CPP1 ( d ). YFP signals were examined by CLSM in protoplasts after agroinfiltration. MAGNESIUM-PROTOPORPHYRIN IX METHYL TRANSFERASE (MgPMT), which is involved in chlorophyll biosynthesis, was used as a negative control. Scale bar, 10 µm ( c ) or 20 µm ( d ). e DAY directly interacts with CPP1 in vitro. Lysates of E. coli cells expressing GST or GST-CPP1 were incubated with MBP or MBP-∆cTP DAY (MBP-∆cTP) immobilized on amylose agarose resins. The resulting eluates were analyzed by immunoblotting using anti-GST antibody. The amount of MBP and MBP-∆cTP used in the pull-down assay were visualized by Coomassie staining.

    Journal: Nature Communications

    Article Title: Chaperone-like protein DAY plays critical roles in photomorphogenesis

    doi: 10.1038/s41467-021-24446-5

    Figure Lengend Snippet: Together with CPP1, DAY regulates the stability of protochlorophyllide oxidoreductase (POR) through its holdase chaperone activity. a Reduction of DAY causes POR instability in N. benthamiana . Virus-induced gene silencing (VIGS) of N. benthamiana DAY ( NbDAY ) was performed. Then the VIGS plants (TRV:NbDAY) were agroinfiltrated with the POR C-GFP construct, and leaf mesophyll cells were examined by CLSM. POR C is a major POR isoform in light-grown Arabidopsis. NbDAY silencing caused a significant reduction in chlorophyll autofluorescence and POR C-GFP fluorescence. Scale bar, 20 µm. b DAY has holdase activity. Absorbance at 340 nm ( A 340 ) was measured at 1 min intervals to quantify turbidity. Thermal aggregation of malate dehydrogenase (2 µM) was measured at 43 °C over 16 min with 0–6 µM of MBP-∆cTP DAY or, as controls, 4 µM MBP-∆cTP/∆JL DAY (del JL) and 10 µM BSA. ∆cTP, deletion of cTP; ∆JL, deletion of the DnaJ- like domain (JL). c , d DAY interacts with POR C and CPP1 in chloroplasts. BiFC assays between DAY and POR C ( c ) or CPP1 ( d ). YFP signals were examined by CLSM in protoplasts after agroinfiltration. MAGNESIUM-PROTOPORPHYRIN IX METHYL TRANSFERASE (MgPMT), which is involved in chlorophyll biosynthesis, was used as a negative control. Scale bar, 10 µm ( c ) or 20 µm ( d ). e DAY directly interacts with CPP1 in vitro. Lysates of E. coli cells expressing GST or GST-CPP1 were incubated with MBP or MBP-∆cTP DAY (MBP-∆cTP) immobilized on amylose agarose resins. The resulting eluates were analyzed by immunoblotting using anti-GST antibody. The amount of MBP and MBP-∆cTP used in the pull-down assay were visualized by Coomassie staining.

    Article Snippet: Anti-Flag (1:5000), α-Tubulin (1:5000), GST (1:5000), and MBP (1:5000) antibodies were purchased from Sigma-Aldrich (A8592, T6199, RPN1236, and M1321).

    Techniques: Activity Assay, Construct, Confocal Laser Scanning Microscopy, Fluorescence, Bimolecular Fluorescence Complementation Assay, Negative Control, In Vitro, Expressing, Incubation, Pull Down Assay, Staining