Journal: Scientific Reports

Article Title: 3D transcranial ultrasound localization microscopy for discrimination between ischemic and hemorrhagic stroke in early phase

doi: 10.1038/s41598-022-18025-x

Figure Lengend Snippet: 3DULM for in vivo angiography at the early phase of stroke’s models. (a) Skin opening for ultrasound imaging. Ischemic model: a small hole was drilled above the MCA branch, and human thrombin was injected. Hemorrhagic model: the parietal bone was drilled, and collagenase was injected in the striatum. ( b) Timeline of the experiment for each model, with four acquisitions: in the half hour prior stroke onset (t−), in hyperacute stage (30 min and 1h30), and the day after with MRI imaging. T2* and T2 MRI sequences slices and 3D rendering. ( c) Ultrasound matrix probe with transducers pooled in four synthetic apertures successively connected to the echograph. ULM processing with filtering, localization and tracking. Rat brain atlas registered on ULM volume (600 µm width slab). 3D rendering with brain surface with Amira software (Thermo Fisher). Scale bars: 1 mm. Figures were created with ( a–c ) Illustrator 2021 ( https://www.adobe.com/products/illustrator ), ( c ) MatLab 2019a ( https://www.mathworks.com/products/matlab ), ( c ) Amira 2019.4 ( https://www.thermofisher.com/amira-avizo ).

Article Snippet: Figures were created with ( a–c ) Illustrator 2021 ( https://www.adobe.com/products/illustrator ), ( c ) MatLab 2019a ( https://www.mathworks.com/products/matlab ), ( c ) Amira 2019.4 ( https://www.thermofisher.com/amira-avizo ).

Techniques: In Vivo, Imaging, Injection, Software

Journal: Scientific Reports

Article Title: 3D transcranial ultrasound localization microscopy for discrimination between ischemic and hemorrhagic stroke in early phase

doi: 10.1038/s41598-022-18025-x

Figure Lengend Snippet: MDI for unsupervised observation reflects the vascularization density. (a) Anatomical regions are extracted from anatomical structures. The hidden areas are excluded from the study. MDI computation with a range of 100 µm on binarized vascularization. ( b) Selection of a round reference region of 1.2 mm diameter in the thalamus and a region of interest in the cortex for ischemic stroke (0.9 mm diameter) and in the striatum for hemorrhage. ( c) Example of a spherical ROI extracted from the ULM volume, binarization and MDI computation. Figures were created with ( a , b ) MatLab 2019a ( https://www.mathworks.com/products/matlab ), ( c ) Amira 2019.4 ( https://www.thermofisher.com/amira-avizo ).

Article Snippet: Figures were created with ( a–c ) Illustrator 2021 ( https://www.adobe.com/products/illustrator ), ( c ) MatLab 2019a ( https://www.mathworks.com/products/matlab ), ( c ) Amira 2019.4 ( https://www.thermofisher.com/amira-avizo ).

Techniques: Selection

Journal: Scientific Reports

Article Title: 3D transcranial ultrasound localization microscopy for discrimination between ischemic and hemorrhagic stroke in early phase

doi: 10.1038/s41598-022-18025-x

Figure Lengend Snippet: The MCA occlusion ischemic stroke was observed with 3DULM at the early phase. (a) 3D renderings at before and 30 min after stroke’s induction (Supplementary Video ). ( b) Sagittal and coronal slices of 600 µm with velocity rendering at different timepoints. Ischemic cortex wrapped with a white dotted line. \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${V}_{max}=30\mathrm{ mm}/\mathrm{s}$$\end{document} V max = 30 mm / s . Upward flows: red, downward flows: blue. ( c) T2 MRI at D + 1 with the segmentation of the lesion (yellow dotted lines). Scale bar: 2 mm. Figures were created with ( a ) Amira 2019.4 ( https://www.thermofisher.com/amira-avizo ), ( b , c ) MatLab 2019a ( https://www.mathworks.com/products/matlab ).

Article Snippet: Figures were created with ( a–c ) Illustrator 2021 ( https://www.adobe.com/products/illustrator ), ( c ) MatLab 2019a ( https://www.mathworks.com/products/matlab ), ( c ) Amira 2019.4 ( https://www.thermofisher.com/amira-avizo ).

Techniques:

Journal: Scientific Reports

Article Title: 3D transcranial ultrasound localization microscopy for discrimination between ischemic and hemorrhagic stroke in early phase

doi: 10.1038/s41598-022-18025-x

Figure Lengend Snippet: Sudden and massive loss of perfusion in the cortex. (a) Coronal slices of MDI with anatomical regions. MDI values inside each region normalized with t−. Scale bar 1 mm, slices of 600 µm. (n = 1). ( b) Projection of the selected ROI in the cortex and thalamus as a reference. c MDI values for the ROI inside ischemia and the reference, normalized with baseline (t−). (Results of the analysis of 1 animal over 4.) Box plot: mean (dot), median (line), 1 and 3 quartile (box), 5 and 95% (whiskers). Figures were created with (a–c). MatLab 2019a ( https://www.mathworks.com/products/matlab ).

Article Snippet: Figures were created with ( a–c ) Illustrator 2021 ( https://www.adobe.com/products/illustrator ), ( c ) MatLab 2019a ( https://www.mathworks.com/products/matlab ), ( c ) Amira 2019.4 ( https://www.thermofisher.com/amira-avizo ).

Techniques:

Journal: Scientific Reports

Article Title: 3D transcranial ultrasound localization microscopy for discrimination between ischemic and hemorrhagic stroke in early phase

doi: 10.1038/s41598-022-18025-x

Figure Lengend Snippet: Collagenase induced hemorrhage in the striatum observed with 3DULM in the early phase. (a) 3D renderings before, and 1h30 after stroke’s induction, and the day after (Supplementary Video ). ( b) Sagittal and coronal slices of 600 µm with velocity rendering at different timepoints. Hemorrhagic core wrapped with a white dotted line. \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${V}_{max}=30\mathrm{ mm}/\mathrm{s}$$\end{document} V max = 30 mm / s . Upward flow: red, downward flows: blue. ( c) T2* MRI at D + 1. Scale bar: 2 mm. Yellow arrow targets the hemorrhagic core. ( d) Projection of the selected ROI in the cortex and thalamus as a reference. ( e) MDI values for the ROI inside the hemorrhagic core and the reference normalized with the baseline. (Results of the analysis of 1 animal over 5.) Figures were created with ( a ) Amira 2019.4 ( https://www.thermofisher.com/amira-avizo ), ( b–e ) MatLab 2019a ( https://www.mathworks.com/products/matlab ).

Article Snippet: Figures were created with ( a–c ) Illustrator 2021 ( https://www.adobe.com/products/illustrator ), ( c ) MatLab 2019a ( https://www.mathworks.com/products/matlab ), ( c ) Amira 2019.4 ( https://www.thermofisher.com/amira-avizo ).

Techniques:

Journal: Scientific Reports

Article Title: 3D transcranial ultrasound localization microscopy for discrimination between ischemic and hemorrhagic stroke in early phase

doi: 10.1038/s41598-022-18025-x

Figure Lengend Snippet: Comparison between ischemic and hemorrhagic models. Variation of the MDI normalized prior onset (t−) for each ROI in the ischemic ( a ) and hemorrhagic ( b ) stroke and reference ROIs. Ratios between the ischemic ( c ) and hemorrhagic ( d ) strokes ROI and the reference region. ( e ) Normalized MDI ratio of the lesion site and the reference region, compared to the baseline (t-). All animals were pooled for baseline ratio in cortex and striatum (n = 9). Student t-test with equal variance ( f ) Volume renderings of ischemic (left) and hemorrhagic (right) strokes 30 min after onset. Scale bar 2 mm. Figures were created with ( a–e ) MatLab 2019a ( https://www.mathworks.com/products/matlab ), ( f ) Amira 2019.4 ( https://www.thermofisher.com/amira-avizo ).

Article Snippet: Figures were created with ( a–c ) Illustrator 2021 ( https://www.adobe.com/products/illustrator ), ( c ) MatLab 2019a ( https://www.mathworks.com/products/matlab ), ( c ) Amira 2019.4 ( https://www.thermofisher.com/amira-avizo ).

Techniques:

Journal: Cell

Article Title: Mapping local and global liquid phase behavior in living cells using photo-oligomerizable seeds

doi: 10.1016/j.cell.2018.10.048

Figure Lengend Snippet: Highlights

Article Snippet: Matlab2017b , Mathworks , https://www.mathworks.com/products/matlab.html.

Techniques: Virus, Recombinant, Transfection, Cloning, Clinical Proteomics, Plasmid Preparation, cDNA Library Assay, Marker, Software