Journal: Cell

Article Title: Deconstruction of corticospinal circuits for goal-directed motor skills

doi: 10.1016/j.cell.2017.08.014

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab1397 0 Rabbit polyclonal anti-RFP Abcam Cat#ab3477 1 Mouse monoclonal anti-NeuN Millipore Cat#MAB37 7 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Goat polyclonal cholinergic acetyltransferase Millipore Cat#AB144 P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Diphtheria toxin Sigma Cat# D0564 Green RetrobeadsTM Lumafluor Cat#N/A Clozapine N-oxide Enzo Life Sciences Cat#BML-NS105-0025 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Mouse/Rosa-LSL-ChR2EYFP The Jackson Laboratory Jax#12569 Mouse/ChAT-Cre The Jackson Laboratory Jax#028861 Mouse/Thy1-ChR2EYFP The Jackson Laboratory Jax#7612 Recombinant DNA AAV-GFP This paper Cat#N/A AAV-FLEX-DTR This paper Cat#N/A AAV-FLEX-PLAP This paper Cat#N/A AAV-syn-FLEX-GCaMP6 UPenn Cat#PV2821 AAV-syn-FLEX-hM4Di-mCitrine Addgene Cat#44362 AAV-CAG-FLEX-GFP Addgene Cat#28304 AAV-EF1α-FLEX-tdTomato Addgene Cat#50462 AAV-tdTomato-P2A-SypEGFP This paper Cat#N/A AAV-FLEX-tdTomato-P2A-SypEGFP Addgene Cat# 51509 AAV-CAG-FLEX-oG-WPRE-SV40pA Addgene Cat#74292 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-mCherry This paper Cat#N/A Lenti-HiRet-Cre This paper Cat#N/A Lenti-HiRet-FLEX-DTR This paper Cat#N/A Rabies-ΔG-mCherry Kim et al., 2016 Cat#N/A Rabies-ΔG-GFP Kim et al., 2016 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms Matlab 2016b Mathworks https://www.mathworks.com/ pClamp 10 Molecular Devices https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software ImageJ2 NIH https://imagej.nih.gov/ij/index.html ImageJ Stabilizer N/A http://www.cs.cmu.edu/~kangli/code/Image_Stabilizer.html Imaris 8.0 Bitplane http://www.bitplane.com/imaris/imaris Matlab CellSort toolbox Matlab central https://www.mathworks.com/matlabcentral/fileexchange/25405-emukamel-cellsort Matlab fastICA toolbox Matlab central https://www.mathworks.com/matlabcentral/linkexchange/links/2115-the-fastica-package-for-matlab Inscopix nVista 2.0.32 Inscopix https://www.inscopix.com/ Mosaic Inscopix https://www.inscopix.com/ Other N/A N/A N/A Open in a separate window KEY RESOURCE TABLE

Techniques: Recombinant, Sequencing, Software

Journal: Current biology : CB

Article Title: Disparate insults relevant to schizophrenia converge on impaired spike synchrony and weaker synaptic interactions in prefrontal local circuits

doi: 10.1016/j.cub.2021.10.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Phencyclidine hydrochloride Sigma-Aldrich P3029 Experimental models: Organisms/strains Rhesus macaque (Macaca mulatta) N/A N/A Mouse: Nestin-Cre mice (strain: B6.Cg (SJL)-TgN(NesCre)1Kln) Jackson Labs Stock #003771 Mouse: Dgcr8flox/flox mice (strain: B6.Cg-Dgcr8tm1.1Blel/Mmjax;) Jackson Labs Stock #0032051 Deposited data NHP and mouse spiking and LFP data This study https://data.mendeley.com/datasets/y9d3yx4xj6/draft?a=270d6c1c-70ec-480d-a727-a64eb7c7d24c Software and algorithms MATLAB MathWorks RRID:SCR_001622 Transfer entropy Matlab Toolbox 38 https://code.google.com/archive/p/transfer-entropy-toolbox/ N/A FieldTrip Matlab Toolbox 114 https://www.fieldtriptoolbox.org/download.php RRID:SCR_004849 Open in a separate window KEY RESOURCES TABLE.

Techniques: Recombinant, Software

Journal: Current biology : CB

Article Title: Disparate insults relevant to schizophrenia converge on impaired spike synchrony and weaker synaptic interactions in prefrontal local circuits

doi: 10.1016/j.cub.2021.10.009

Figure Lengend Snippet: Influence of drug condition (A, B; monkeys) and genotype (C, D; mice) on LFP power (A, C) and pairwise phase consistency (PPC) between spikes and LFPs (B, D). LFP signals were convolved with a set of complex Morlet wavelets in the frequency range of 1–100 Hz using the Matlab FieldTrip114 toolbox. After assigning phase angles to each spike (relative to LFP oscillations at a given frequency), PPC is defined as the mean cosine between the phase angles of all possible pairs of spikes, capturing the tendency for spikes to occur at consistent phases of LFP oscillations64. (A, C) Mean LFP power spectra in monkeys (A) and mice (C). LFP power was significantly reduced by blocking NMDAR in monkeys and hemizygous deletion of Dgcr8 in mice (black dots above horizontal axis indicate p < 0.05 between conditions by t-test at each frequency, FDR corrected for the number of tests using the Benjamini-Hochberg method). Error bars indicate SEM over LFP channels (electrodes). The dip at 60 Hz in the monkey power spectra reflects notch filtering. (B, D) Median PPC as a function of frequency in monkeys (B) and mice (D). PPC was significantly reduced by blocking NMDAR in monkeys (NSaline = 960 neurons, NDrug= 763 neurons) and hemizygous deletion of Dgcr8 in mice (NWT = 79 neurons, NDgcr8 = 128 neurons) (black dots above horizontal axis indicate p < 0.05 between conditions by Wilcoxon Rank Sum test at each frequency, FDR corrected for the number of tests using the Benjamini-Hochberg method).

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Phencyclidine hydrochloride Sigma-Aldrich P3029 Experimental models: Organisms/strains Rhesus macaque (Macaca mulatta) N/A N/A Mouse: Nestin-Cre mice (strain: B6.Cg (SJL)-TgN(NesCre)1Kln) Jackson Labs Stock #003771 Mouse: Dgcr8flox/flox mice (strain: B6.Cg-Dgcr8tm1.1Blel/Mmjax;) Jackson Labs Stock #0032051 Deposited data NHP and mouse spiking and LFP data This study https://data.mendeley.com/datasets/y9d3yx4xj6/draft?a=270d6c1c-70ec-480d-a727-a64eb7c7d24c Software and algorithms MATLAB MathWorks RRID:SCR_001622 Transfer entropy Matlab Toolbox 38 https://code.google.com/archive/p/transfer-entropy-toolbox/ N/A FieldTrip Matlab Toolbox 114 https://www.fieldtriptoolbox.org/download.php RRID:SCR_004849 Open in a separate window KEY RESOURCES TABLE.

Techniques: Blocking Assay

Journal: Current biology : CB

Article Title: Disparate insults relevant to schizophrenia converge on impaired spike synchrony and weaker synaptic interactions in prefrontal local circuits

doi: 10.1016/j.cub.2021.10.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Phencyclidine hydrochloride Sigma-Aldrich P3029 Experimental models: Organisms/strains Rhesus macaque (Macaca mulatta) N/A N/A Mouse: Nestin-Cre mice (strain: B6.Cg (SJL)-TgN(NesCre)1Kln) Jackson Labs Stock #003771 Mouse: Dgcr8flox/flox mice (strain: B6.Cg-Dgcr8tm1.1Blel/Mmjax;) Jackson Labs Stock #0032051 Deposited data NHP and mouse spiking and LFP data This study https://data.mendeley.com/datasets/y9d3yx4xj6/draft?a=270d6c1c-70ec-480d-a727-a64eb7c7d24c Software and algorithms MATLAB MathWorks RRID:SCR_001622 Transfer entropy Matlab Toolbox 38 https://code.google.com/archive/p/transfer-entropy-toolbox/ N/A FieldTrip Matlab Toolbox 114 https://www.fieldtriptoolbox.org/download.php RRID:SCR_004849 Open in a separate window KEY RESOURCES TABLE.

Techniques: Recombinant, Software