Journal:

Article Title: Analysis of Mycobacterium Species for the Presence of a Macrolide Toxin, Mycolactone

doi: 10.1128/IAI.72.1.123-132.2004

Figure Lengend Snippet: Mycobacterium species used in this study and the results of the ASL cytopathicity assays for the slow-growing mycobacteria a

Article Snippet: These results show that neither mycolactone nor the core mycolactone lipid was present as cell-associated lipids in any of the mycobacterial isolates tested other than M. ulcerans . fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 2. caption a7 Effect of ASLs on L929 fibroblasts at 48 h. Original magnification, ×200. (A) Control monolayer. (B) Effect of 1615 ASLs at 2.12 ng/ml. (C) CEI 01-628 ASLs (10,000 μg). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Isolate, species Source CPA c of ASLs (μg/ml) 1615, M. ulcerans (ATCC 35840) TMCC 0.0021 706, M. avium (ATCC 35714) TMCC — d K10, M. paratuberculosis NADC b — 1464, M. intracellulare (ATCC 35768) TMCC — 1467, M. intracellulare (ATCC 35770) TMCC — 1456, M. gastri (ATCC 15754) TMCC — 1318, M. gordonae (ATCC 35756) TMCC — 1319, M. gordonae (ATCC 35757) TMCC — 1324, M. gordonae (ATCC 14470) TMCC 10,000 1203, M. kansasii (ATCC 35776) TMCC 1,250 1204, M. kansasii (ATCC 12478) TMCC 5,000 1214, M. kansasii (ATCC 35777) TMCC — 1601, M. microti (ATCC 35781) TMCC 10,000 1302, M. scrofulaceum (ATCC 35785) TMCC — 1305, M. scrofulaceum (ATCC 35786) TMCC 10,000 1306, M. scrofulaceum (ATCC 35787) TMCC 10,000 1307, M. scrofulaceum (ATCC 35788) TMCC 20,000 1312, M. scrofulaceum (ATCC 35790) TMCC 10,000 1314, M. scrofulaceum (ATCC 35791) TMCC 20,000 1315, M. scrofulaceum (ATCC 35792) TMCC 20,000 1316, M. scrofulaceum (ATCC 35793) TMCC — 1320, M. scrofulaceum (ATCC 35794) TMCC — 1321, M. scrofulaceum (ATCC 35795) TMCC — 1323, M. scrofulaceum (ATCC 19981) TMCC — 1481, M. nonchromogenicium (ATCC 19530) TMCC — 1541, M. flavescens (ATCC 14474) CEI — 01-626 CEI 2,500 01-627 CEI 2,500 01-628 CEI 10,000 01-629 CEI — 01-630 CEI — 01-631 CEI 1,600 01-632 CEI 10,000 01-633 CEI — 01-634 CEI — 01-635 CEI 2,500 01-636 CEI 2,500 01-664 CEI 330 01-665 CEI — 01-666 CEI — 01-667 CEI — 01-668 Open in a separate window a Cytopathicity of ASLs of a chloroform-methanol (2:1, vol/vol) extract from Mycobacterium spp. on L929 fibroblasts after 48 h. b NADC, National Animal Disease Center, Ames, Iowa. c CPA is defined as the minimal amount of ASLs added to cause altered morphology of 90% of the cells and detachment within 48 h. d —, no cytopathic effect seen at <20 μg/μl.

Techniques:

Journal: STAR Protocols

Article Title: High-throughput generation of midbrain dopaminergic neuron organoids from reporter human pluripotent stem cells

doi: 10.1016/j.xpro.2021.100463

Figure Lengend Snippet:

Article Snippet: Fontana-Masson Stain Kit (Melanin Stain) , Abcam , Cat#Ab150669; RRID: N/A.

Techniques: Recombinant, Lysis, Staining, Transfection, Plasmid Preparation, Selection, CRISPR, Software, Cell Culture, Cell Counting