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Image Search Results
Journal: Science translational medicine
Article Title: Nonlesional lupus skin contributes to inflammatory education of myeloid cells and primes for cutaneous inflammation.
doi: 10.1126/scitranslmed.abn2263
Figure Lengend Snippet: Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass cytometry. Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Article Snippet:
Techniques: Control, Staining, Expressing, Generated, Imaging, Mass Cytometry