map2k6 Search Results


gene exp map2k6 mm00803694 m1  (Thermo Fisher)
85
Thermo Fisher gene exp map2k6 mm00803694 m1
Chromatin immunoprecipitation (ChIP) in BAT of male wt and p62 Δ69-251 mice ( a ) and p62 −/− mice ( b ) using antibodies specific for ATF2. ChIP-qPCR analysis of Foxl2 (negative control), Jun , Fos , Atf3 , Ucp1 (CRE2) , Ucp1 (CRE4) , and Pgc-1α ( n = 5 mice pooled each genotype, n = 2 technical replicates) ( a , b ). Luciferase-based CRE reporter assay of HEK293T cells transfected with the pCRE-Luc reporter, a dominant positive <t>MKK6</t> (K82A) and p62 wt or p62 Δ69-251 ( n = 7/8 technical replicates each group) ( c ). Panel ( c ) is a representative example of three independently performed studies, each yielding similar results. Data in ( a ) and ( b ) have been analyzed using a two-sided two-tailed t -test, data in ( c ) have been analyzed using one-way ANOVA was treatment as co-variant and using Bonferroni multiple comparison between the treatment groups. Data represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. Exact p -values are ( c ) pCRE-Luc vs. pCRE-Luc + MKK6 + p62 wt p < 0.0001, pCRE-Luc vs. pCRE-Luc + MKK6 + p62 Δ69-251 p > 0.05, pCRE-Luc vs. pCRE-Luc + MKK6 + SB203580 p > 0.05, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + p62 Δ69-251 p < 0.0001, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + SB203580 p > 0.0001, pCRE-Luc + MKK6 + p62 Δ69-251 vs. pCRE-Luc + MKK6 + SB203580 p = 0.0005. Foxl2 forkhead box L2, Fos FBJ osteosarcoma oncogene, Atf3 activating transcription factor 3.
Gene Exp Map2k6 Mm00803694 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
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kinase inactive mutant mkk6  (Carna Inc)
94
Carna Inc kinase inactive mutant mkk6
Chromatin immunoprecipitation (ChIP) in BAT of male wt and p62 Δ69-251 mice ( a ) and p62 −/− mice ( b ) using antibodies specific for ATF2. ChIP-qPCR analysis of Foxl2 (negative control), Jun , Fos , Atf3 , Ucp1 (CRE2) , Ucp1 (CRE4) , and Pgc-1α ( n = 5 mice pooled each genotype, n = 2 technical replicates) ( a , b ). Luciferase-based CRE reporter assay of HEK293T cells transfected with the pCRE-Luc reporter, a dominant positive <t>MKK6</t> (K82A) and p62 wt or p62 Δ69-251 ( n = 7/8 technical replicates each group) ( c ). Panel ( c ) is a representative example of three independently performed studies, each yielding similar results. Data in ( a ) and ( b ) have been analyzed using a two-sided two-tailed t -test, data in ( c ) have been analyzed using one-way ANOVA was treatment as co-variant and using Bonferroni multiple comparison between the treatment groups. Data represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. Exact p -values are ( c ) pCRE-Luc vs. pCRE-Luc + MKK6 + p62 wt p < 0.0001, pCRE-Luc vs. pCRE-Luc + MKK6 + p62 Δ69-251 p > 0.05, pCRE-Luc vs. pCRE-Luc + MKK6 + SB203580 p > 0.05, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + p62 Δ69-251 p < 0.0001, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + SB203580 p > 0.0001, pCRE-Luc + MKK6 + p62 Δ69-251 vs. pCRE-Luc + MKK6 + SB203580 p = 0.0005. Foxl2 forkhead box L2, Fos FBJ osteosarcoma oncogene, Atf3 activating transcription factor 3.
Kinase Inactive Mutant Mkk6, supplied by Carna Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mkk6  (OriGene)
90
OriGene mkk6
Chromatin immunoprecipitation (ChIP) in BAT of male wt and p62 Δ69-251 mice ( a ) and p62 −/− mice ( b ) using antibodies specific for ATF2. ChIP-qPCR analysis of Foxl2 (negative control), Jun , Fos , Atf3 , Ucp1 (CRE2) , Ucp1 (CRE4) , and Pgc-1α ( n = 5 mice pooled each genotype, n = 2 technical replicates) ( a , b ). Luciferase-based CRE reporter assay of HEK293T cells transfected with the pCRE-Luc reporter, a dominant positive <t>MKK6</t> (K82A) and p62 wt or p62 Δ69-251 ( n = 7/8 technical replicates each group) ( c ). Panel ( c ) is a representative example of three independently performed studies, each yielding similar results. Data in ( a ) and ( b ) have been analyzed using a two-sided two-tailed t -test, data in ( c ) have been analyzed using one-way ANOVA was treatment as co-variant and using Bonferroni multiple comparison between the treatment groups. Data represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. Exact p -values are ( c ) pCRE-Luc vs. pCRE-Luc + MKK6 + p62 wt p < 0.0001, pCRE-Luc vs. pCRE-Luc + MKK6 + p62 Δ69-251 p > 0.05, pCRE-Luc vs. pCRE-Luc + MKK6 + SB203580 p > 0.05, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + p62 Δ69-251 p < 0.0001, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + SB203580 p > 0.0001, pCRE-Luc + MKK6 + p62 Δ69-251 vs. pCRE-Luc + MKK6 + SB203580 p = 0.0005. Foxl2 forkhead box L2, Fos FBJ osteosarcoma oncogene, Atf3 activating transcription factor 3.
Mkk6, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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gene exp map2k6 hs00992389 m1  (Thermo Fisher)
91
Thermo Fisher gene exp map2k6 hs00992389 m1
Chromatin immunoprecipitation (ChIP) in BAT of male wt and p62 Δ69-251 mice ( a ) and p62 −/− mice ( b ) using antibodies specific for ATF2. ChIP-qPCR analysis of Foxl2 (negative control), Jun , Fos , Atf3 , Ucp1 (CRE2) , Ucp1 (CRE4) , and Pgc-1α ( n = 5 mice pooled each genotype, n = 2 technical replicates) ( a , b ). Luciferase-based CRE reporter assay of HEK293T cells transfected with the pCRE-Luc reporter, a dominant positive <t>MKK6</t> (K82A) and p62 wt or p62 Δ69-251 ( n = 7/8 technical replicates each group) ( c ). Panel ( c ) is a representative example of three independently performed studies, each yielding similar results. Data in ( a ) and ( b ) have been analyzed using a two-sided two-tailed t -test, data in ( c ) have been analyzed using one-way ANOVA was treatment as co-variant and using Bonferroni multiple comparison between the treatment groups. Data represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. Exact p -values are ( c ) pCRE-Luc vs. pCRE-Luc + MKK6 + p62 wt p < 0.0001, pCRE-Luc vs. pCRE-Luc + MKK6 + p62 Δ69-251 p > 0.05, pCRE-Luc vs. pCRE-Luc + MKK6 + SB203580 p > 0.05, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + p62 Δ69-251 p < 0.0001, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + SB203580 p > 0.0001, pCRE-Luc + MKK6 + p62 Δ69-251 vs. pCRE-Luc + MKK6 + SB203580 p = 0.0005. Foxl2 forkhead box L2, Fos FBJ osteosarcoma oncogene, Atf3 activating transcription factor 3.
Gene Exp Map2k6 Hs00992389 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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p mkk3 mkk6  (Proteintech)
93
Proteintech p mkk3 mkk6
Chromatin immunoprecipitation (ChIP) in BAT of male wt and p62 Δ69-251 mice ( a ) and p62 −/− mice ( b ) using antibodies specific for ATF2. ChIP-qPCR analysis of Foxl2 (negative control), Jun , Fos , Atf3 , Ucp1 (CRE2) , Ucp1 (CRE4) , and Pgc-1α ( n = 5 mice pooled each genotype, n = 2 technical replicates) ( a , b ). Luciferase-based CRE reporter assay of HEK293T cells transfected with the pCRE-Luc reporter, a dominant positive <t>MKK6</t> (K82A) and p62 wt or p62 Δ69-251 ( n = 7/8 technical replicates each group) ( c ). Panel ( c ) is a representative example of three independently performed studies, each yielding similar results. Data in ( a ) and ( b ) have been analyzed using a two-sided two-tailed t -test, data in ( c ) have been analyzed using one-way ANOVA was treatment as co-variant and using Bonferroni multiple comparison between the treatment groups. Data represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. Exact p -values are ( c ) pCRE-Luc vs. pCRE-Luc + MKK6 + p62 wt p < 0.0001, pCRE-Luc vs. pCRE-Luc + MKK6 + p62 Δ69-251 p > 0.05, pCRE-Luc vs. pCRE-Luc + MKK6 + SB203580 p > 0.05, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + p62 Δ69-251 p < 0.0001, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + SB203580 p > 0.0001, pCRE-Luc + MKK6 + p62 Δ69-251 vs. pCRE-Luc + MKK6 + SB203580 p = 0.0005. Foxl2 forkhead box L2, Fos FBJ osteosarcoma oncogene, Atf3 activating transcription factor 3.
P Mkk3 Mkk6, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chicken ovalbumin  (Addgene inc)
93
Addgene inc chicken ovalbumin
Chromatin immunoprecipitation (ChIP) in BAT of male wt and p62 Δ69-251 mice ( a ) and p62 −/− mice ( b ) using antibodies specific for ATF2. ChIP-qPCR analysis of Foxl2 (negative control), Jun , Fos , Atf3 , Ucp1 (CRE2) , Ucp1 (CRE4) , and Pgc-1α ( n = 5 mice pooled each genotype, n = 2 technical replicates) ( a , b ). Luciferase-based CRE reporter assay of HEK293T cells transfected with the pCRE-Luc reporter, a dominant positive <t>MKK6</t> (K82A) and p62 wt or p62 Δ69-251 ( n = 7/8 technical replicates each group) ( c ). Panel ( c ) is a representative example of three independently performed studies, each yielding similar results. Data in ( a ) and ( b ) have been analyzed using a two-sided two-tailed t -test, data in ( c ) have been analyzed using one-way ANOVA was treatment as co-variant and using Bonferroni multiple comparison between the treatment groups. Data represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. Exact p -values are ( c ) pCRE-Luc vs. pCRE-Luc + MKK6 + p62 wt p < 0.0001, pCRE-Luc vs. pCRE-Luc + MKK6 + p62 Δ69-251 p > 0.05, pCRE-Luc vs. pCRE-Luc + MKK6 + SB203580 p > 0.05, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + p62 Δ69-251 p < 0.0001, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + SB203580 p > 0.0001, pCRE-Luc + MKK6 + p62 Δ69-251 vs. pCRE-Luc + MKK6 + SB203580 p = 0.0005. Foxl2 forkhead box L2, Fos FBJ osteosarcoma oncogene, Atf3 activating transcription factor 3.
Chicken Ovalbumin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chicken ovalbumin/product/Addgene inc
Average 93 stars, based on 1 article reviews
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gene exp map2k6 hs00177150 m1  (Thermo Fisher)
85
Thermo Fisher gene exp map2k6 hs00177150 m1
Chromatin immunoprecipitation (ChIP) in BAT of male wt and p62 Δ69-251 mice ( a ) and p62 −/− mice ( b ) using antibodies specific for ATF2. ChIP-qPCR analysis of Foxl2 (negative control), Jun , Fos , Atf3 , Ucp1 (CRE2) , Ucp1 (CRE4) , and Pgc-1α ( n = 5 mice pooled each genotype, n = 2 technical replicates) ( a , b ). Luciferase-based CRE reporter assay of HEK293T cells transfected with the pCRE-Luc reporter, a dominant positive <t>MKK6</t> (K82A) and p62 wt or p62 Δ69-251 ( n = 7/8 technical replicates each group) ( c ). Panel ( c ) is a representative example of three independently performed studies, each yielding similar results. Data in ( a ) and ( b ) have been analyzed using a two-sided two-tailed t -test, data in ( c ) have been analyzed using one-way ANOVA was treatment as co-variant and using Bonferroni multiple comparison between the treatment groups. Data represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. Exact p -values are ( c ) pCRE-Luc vs. pCRE-Luc + MKK6 + p62 wt p < 0.0001, pCRE-Luc vs. pCRE-Luc + MKK6 + p62 Δ69-251 p > 0.05, pCRE-Luc vs. pCRE-Luc + MKK6 + SB203580 p > 0.05, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + p62 Δ69-251 p < 0.0001, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + SB203580 p > 0.0001, pCRE-Luc + MKK6 + p62 Δ69-251 vs. pCRE-Luc + MKK6 + SB203580 p = 0.0005. Foxl2 forkhead box L2, Fos FBJ osteosarcoma oncogene, Atf3 activating transcription factor 3.
Gene Exp Map2k6 Hs00177150 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
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85/100 stars
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gene exp map2k6 cf02683089 m1  (Thermo Fisher)
86
Thermo Fisher gene exp map2k6 cf02683089 m1
Chromatin immunoprecipitation (ChIP) in BAT of male wt and p62 Δ69-251 mice ( a ) and p62 −/− mice ( b ) using antibodies specific for ATF2. ChIP-qPCR analysis of Foxl2 (negative control), Jun , Fos , Atf3 , Ucp1 (CRE2) , Ucp1 (CRE4) , and Pgc-1α ( n = 5 mice pooled each genotype, n = 2 technical replicates) ( a , b ). Luciferase-based CRE reporter assay of HEK293T cells transfected with the pCRE-Luc reporter, a dominant positive <t>MKK6</t> (K82A) and p62 wt or p62 Δ69-251 ( n = 7/8 technical replicates each group) ( c ). Panel ( c ) is a representative example of three independently performed studies, each yielding similar results. Data in ( a ) and ( b ) have been analyzed using a two-sided two-tailed t -test, data in ( c ) have been analyzed using one-way ANOVA was treatment as co-variant and using Bonferroni multiple comparison between the treatment groups. Data represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. Exact p -values are ( c ) pCRE-Luc vs. pCRE-Luc + MKK6 + p62 wt p < 0.0001, pCRE-Luc vs. pCRE-Luc + MKK6 + p62 Δ69-251 p > 0.05, pCRE-Luc vs. pCRE-Luc + MKK6 + SB203580 p > 0.05, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + p62 Δ69-251 p < 0.0001, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + SB203580 p > 0.0001, pCRE-Luc + MKK6 + p62 Δ69-251 vs. pCRE-Luc + MKK6 + SB203580 p = 0.0005. Foxl2 forkhead box L2, Fos FBJ osteosarcoma oncogene, Atf3 activating transcription factor 3.
Gene Exp Map2k6 Cf02683089 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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mg204942  (OriGene)
91
OriGene mg204942
Chromatin immunoprecipitation (ChIP) in BAT of male wt and p62 Δ69-251 mice ( a ) and p62 −/− mice ( b ) using antibodies specific for ATF2. ChIP-qPCR analysis of Foxl2 (negative control), Jun , Fos , Atf3 , Ucp1 (CRE2) , Ucp1 (CRE4) , and Pgc-1α ( n = 5 mice pooled each genotype, n = 2 technical replicates) ( a , b ). Luciferase-based CRE reporter assay of HEK293T cells transfected with the pCRE-Luc reporter, a dominant positive <t>MKK6</t> (K82A) and p62 wt or p62 Δ69-251 ( n = 7/8 technical replicates each group) ( c ). Panel ( c ) is a representative example of three independently performed studies, each yielding similar results. Data in ( a ) and ( b ) have been analyzed using a two-sided two-tailed t -test, data in ( c ) have been analyzed using one-way ANOVA was treatment as co-variant and using Bonferroni multiple comparison between the treatment groups. Data represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. Exact p -values are ( c ) pCRE-Luc vs. pCRE-Luc + MKK6 + p62 wt p < 0.0001, pCRE-Luc vs. pCRE-Luc + MKK6 + p62 Δ69-251 p > 0.05, pCRE-Luc vs. pCRE-Luc + MKK6 + SB203580 p > 0.05, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + p62 Δ69-251 p < 0.0001, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + SB203580 p > 0.0001, pCRE-Luc + MKK6 + p62 Δ69-251 vs. pCRE-Luc + MKK6 + SB203580 p = 0.0005. Foxl2 forkhead box L2, Fos FBJ osteosarcoma oncogene, Atf3 activating transcription factor 3.
Mg204942, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp map2k6 rn00586764 m1  (Thermo Fisher)
85
Thermo Fisher gene exp map2k6 rn00586764 m1
Quantitative RT-PCT validation of microarray results on selected gene transcripts.
Gene Exp Map2k6 Rn00586764 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mice haploinsufficient for map2k6  (Jackson Laboratory)
90
Jackson Laboratory mice haploinsufficient for map2k6
Quantitative RT-PCT validation of microarray results on selected gene transcripts.
Mice Haploinsufficient For Map2k6, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


Chromatin immunoprecipitation (ChIP) in BAT of male wt and p62 Δ69-251 mice ( a ) and p62 −/− mice ( b ) using antibodies specific for ATF2. ChIP-qPCR analysis of Foxl2 (negative control), Jun , Fos , Atf3 , Ucp1 (CRE2) , Ucp1 (CRE4) , and Pgc-1α ( n = 5 mice pooled each genotype, n = 2 technical replicates) ( a , b ). Luciferase-based CRE reporter assay of HEK293T cells transfected with the pCRE-Luc reporter, a dominant positive MKK6 (K82A) and p62 wt or p62 Δ69-251 ( n = 7/8 technical replicates each group) ( c ). Panel ( c ) is a representative example of three independently performed studies, each yielding similar results. Data in ( a ) and ( b ) have been analyzed using a two-sided two-tailed t -test, data in ( c ) have been analyzed using one-way ANOVA was treatment as co-variant and using Bonferroni multiple comparison between the treatment groups. Data represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. Exact p -values are ( c ) pCRE-Luc vs. pCRE-Luc + MKK6 + p62 wt p < 0.0001, pCRE-Luc vs. pCRE-Luc + MKK6 + p62 Δ69-251 p > 0.05, pCRE-Luc vs. pCRE-Luc + MKK6 + SB203580 p > 0.05, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + p62 Δ69-251 p < 0.0001, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + SB203580 p > 0.0001, pCRE-Luc + MKK6 + p62 Δ69-251 vs. pCRE-Luc + MKK6 + SB203580 p = 0.0005. Foxl2 forkhead box L2, Fos FBJ osteosarcoma oncogene, Atf3 activating transcription factor 3.

Journal: Nature Communications

Article Title: The scaffold protein p62 regulates adaptive thermogenesis through ATF2 nuclear target activation

doi: 10.1038/s41467-020-16230-8

Figure Lengend Snippet: Chromatin immunoprecipitation (ChIP) in BAT of male wt and p62 Δ69-251 mice ( a ) and p62 −/− mice ( b ) using antibodies specific for ATF2. ChIP-qPCR analysis of Foxl2 (negative control), Jun , Fos , Atf3 , Ucp1 (CRE2) , Ucp1 (CRE4) , and Pgc-1α ( n = 5 mice pooled each genotype, n = 2 technical replicates) ( a , b ). Luciferase-based CRE reporter assay of HEK293T cells transfected with the pCRE-Luc reporter, a dominant positive MKK6 (K82A) and p62 wt or p62 Δ69-251 ( n = 7/8 technical replicates each group) ( c ). Panel ( c ) is a representative example of three independently performed studies, each yielding similar results. Data in ( a ) and ( b ) have been analyzed using a two-sided two-tailed t -test, data in ( c ) have been analyzed using one-way ANOVA was treatment as co-variant and using Bonferroni multiple comparison between the treatment groups. Data represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. Exact p -values are ( c ) pCRE-Luc vs. pCRE-Luc + MKK6 + p62 wt p < 0.0001, pCRE-Luc vs. pCRE-Luc + MKK6 + p62 Δ69-251 p > 0.05, pCRE-Luc vs. pCRE-Luc + MKK6 + SB203580 p > 0.05, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + p62 Δ69-251 p < 0.0001, pCRE-Luc + MKK6 + p62 wt vs. pCRE-Luc + MKK6 + SB203580 p > 0.0001, pCRE-Luc + MKK6 + p62 Δ69-251 vs. pCRE-Luc + MKK6 + SB203580 p = 0.0005. Foxl2 forkhead box L2, Fos FBJ osteosarcoma oncogene, Atf3 activating transcription factor 3.

Article Snippet: Hpr t Mm01545399_m1, Jun Mm00495062_s, Fos Mm00487425_m1, JunB Mm04243546_s1, Atf3 Mm00476033_m1, Atm Mm01177457_m1, Xpa Mm00457111_m1, Rad23b Mm00772280_m1, Msh6 Mm00487761_m1, Nos2 Mm00440502_m1, Hspa5 Mm00517691_m1, Ccna1 Mm01292554_m1, Ccnd1 Mm00432359_m1, Tnf Mm00443258_m1, Il8 Mm00441263_m1, Il1b Mm00434228_m1, Il6 Mm00446190_m1, Th Mm00447557_m1, Col1a1 Mm00801666_g1, Mmp2 Mm00439498_m1, Sele Mm00441278_m1, Selp Mm01295931_m1, Vcam1 Mm01320970_m1, Ppargc1 Mm01208835_m1, Ucp1 Mm01244861_m1, Zfp516 Mm00813584_m1, Mapk14 Mm01301009_m1, Creb1 Mm00501607_m1, Brca1 Mm00515386_m1, Bcl2L1 Mm00437783_m1, Ddit3 Mm01135937_g1, Adrb2 Mm02524224_s1, Map2k6 Mm00803694_m1, Ep300 Mm00625535_m1, Bcl6 Mm00477633_m1, Nfil3 Mm00600292_s1, Pdgfra Mm00440701_m1, Atf6 Mm01295319_m1, Atf4 Mm00515325_g1, Ulk1 Mm00437238_m1, Atg7 Mm00512209_m1, Atf13 Mm00521135_m1, Map1lc3a Mm00458724_m1.

Techniques: Chromatin Immunoprecipitation, ChIP-qPCR, Negative Control, Luciferase, Reporter Assay, Transfection, Two Tailed Test, Variant Assay, Comparison

Quantitative RT-PCT validation of microarray results on selected gene transcripts.

Journal: International Journal of Molecular Sciences

Article Title: Global Transcriptomic Profiling Using Small Volumes of Whole Blood: A Cost-Effective Method for Translational Genomic Biomarker Identification in Small Animals

doi: 10.3390/ijms12042502

Figure Lengend Snippet: Quantitative RT-PCT validation of microarray results on selected gene transcripts.

Article Snippet: The following primers and probes were selected from the Applied Biosystems Assays-on-Demand gene expression products: Tcf7 (Rn_00493446_m1), Map2k6 (Rn_00586764_m1), Nrp1 (Rn_00595457_m1), Alas2 (Rn_00566201_m1), Flad1 (Rn_01512487_m1), Canx (Rn_00596877_m1), S 100a9 (Rn0585879_m1), Tnf (Rn_99999017_m1), Il1b (Rn_01514151_m1), and 18S (Hs_99999901_s1).

Techniques: Biomarker Discovery, Microarray