Journal: Folia Microbiologica

Article Title: Atypical URA5 gene restriction fragment length polymorphism banding profile in Cryptococcus neoformans strains

doi: 10.1007/s12223-019-00699-y

Figure Lengend Snippet: Restriction map of K1b strain URA 5 gene prepared using BioEdit v7.2.0 (top); comparison of CBS 10079 and K1b strains’ sequence fragments. In K1b sequence point mutation (bold; position 374) as well as recognition sequences in the new restriction site are indicated (bottom)

Article Snippet: Restriction maps of the tested and reference strains were prepared using BioEdit v7.2.0 software.

Techniques: Sequencing, Mutagenesis

Journal: International journal of mycobacteriology

Article Title: Molecular epidemiology of Mycobacterium avium subspecies paratuberculosis in ruminants in different parts of India.

doi: 10.1016/j.ijmyco.2015.11.003

Figure Lengend Snippet: Fig. 1 – IS900 PCR. Lane M, marker; lane 1, C-132; lane 2, C1A; lane 3, 163A; lane 4, CS2028; lane 5, K10 MAP. Note: MAP = Mycobacterium avium subspecies paratuberculosis; PCR = polymerase chain reaction.

Article Snippet: The cattle ‘C’ type was detected only in seven cattle isolates from the Bareilly region (North India) and produced four bands by PCR-REA (67 bp, 218 bp, 285 bp, and 323 bp). ogy of Mycobacterium avium subspecies paratuberculosis in ruminants /10.1016/j.ijmyco.2015.11.003 All MAP reference strains (K10, ATCC 19698, and 316F) were ‘C’ type, while M. avium (M120/04 UK) showed an Maa-type REA pattern as expected.

Techniques: Marker, Polymerase Chain Reaction

Journal: International journal of mycobacteriology

Article Title: Molecular epidemiology of Mycobacterium avium subspecies paratuberculosis in ruminants in different parts of India.

doi: 10.1016/j.ijmyco.2015.11.003

Figure Lengend Snippet: Fig. 2 – IS1311 gene PCR. Lane M, marker; lane 1, C-132; lane 2, C1A; lane 3, 163A; lane 4, CS2028; lane 5, C16; lane 6, H615; lane 7, K10 MAP. Note: MAP = Mycobacterium avium subspecies paratuberculosis; PCR = polymerase chain reaction.

Article Snippet: The cattle ‘C’ type was detected only in seven cattle isolates from the Bareilly region (North India) and produced four bands by PCR-REA (67 bp, 218 bp, 285 bp, and 323 bp). ogy of Mycobacterium avium subspecies paratuberculosis in ruminants /10.1016/j.ijmyco.2015.11.003 All MAP reference strains (K10, ATCC 19698, and 316F) were ‘C’ type, while M. avium (M120/04 UK) showed an Maa-type REA pattern as expected.

Techniques: Marker, Polymerase Chain Reaction

Journal: International journal of mycobacteriology

Article Title: Molecular epidemiology of Mycobacterium avium subspecies paratuberculosis in ruminants in different parts of India.

doi: 10.1016/j.ijmyco.2015.11.003

Figure Lengend Snippet: Fig. 3 – PCR-REA analysis of MAP isolates. Lane M1 and M2, markers; lane 1, C-139 (B type); lane 2, C-12A (B type); lane 3, 316FMTCC (C type); lane 4, C-148A (C type); lane 5, 1168A (B type); lane 6, Mycobacterium bovis (no band pattern). Note: MAP = Mycobacterium avium subspecies paratuberculosis; PCR = polymerase chain reaction; REA = restriction endonuclease analysis.

Article Snippet: The cattle ‘C’ type was detected only in seven cattle isolates from the Bareilly region (North India) and produced four bands by PCR-REA (67 bp, 218 bp, 285 bp, and 323 bp). ogy of Mycobacterium avium subspecies paratuberculosis in ruminants /10.1016/j.ijmyco.2015.11.003 All MAP reference strains (K10, ATCC 19698, and 316F) were ‘C’ type, while M. avium (M120/04 UK) showed an Maa-type REA pattern as expected.

Techniques: Polymerase Chain Reaction

Journal: International journal of mycobacteriology

Article Title: Molecular epidemiology of Mycobacterium avium subspecies paratuberculosis in ruminants in different parts of India.

doi: 10.1016/j.ijmyco.2015.11.003

Figure Lengend Snippet: Fig. 4 – PCR-REA analysis of MAP isolates. Lane M1 and M2, marker; lane 1, 163A (B type); lane 2, C-132 (B type); lane3, CS2280 (B type); lane 4, CS1700 (B type); lane 5, C 93 (B type); lane 6, C260 (B type). Note: MAP = Mycobacterium avium subspecies paratuberculosis; PCR = polymerase chain reaction; REA = restriction endonuclease analysis.

Article Snippet: The cattle ‘C’ type was detected only in seven cattle isolates from the Bareilly region (North India) and produced four bands by PCR-REA (67 bp, 218 bp, 285 bp, and 323 bp). ogy of Mycobacterium avium subspecies paratuberculosis in ruminants /10.1016/j.ijmyco.2015.11.003 All MAP reference strains (K10, ATCC 19698, and 316F) were ‘C’ type, while M. avium (M120/04 UK) showed an Maa-type REA pattern as expected.

Techniques: Marker, Polymerase Chain Reaction

Journal: Canadian Journal of Veterinary Research

Article Title: Development of an immunocapture-polymerase chain reaction assay using IgY to detect Mycobacterium avium subsp. paratuberculosis

doi:

Figure Lengend Snippet: Determination of Beads A volume required for immunocapture of American Type Culture Collection (ATCC) strain 19698 of Mycobacterium avium subsp. paratuberculosis (MAP). For each Beads A volume used, DNA was run undiluted (N) and at dilutions of 1:10 (−1) and 1:100 (−2). The bead volumes were as follows: lanes 1 to 3, 10 μL; lanes 4 to 6, 20 μL; lanes 7 to 9, 30 μL; lanes 10 to 12, 40 μL. Lane −C — negative water control; lane M — 1-kb molecular weight marker; bp — base pairs.

Article Snippet: The concentrations of 3 field and 2 reference strains of MAP [11992, EA4146, 2945, American Type Culture Collection (ATCC) 12258, and ATCC 19698] were adjusted to 2 × 10 7 cells/mL.

Techniques: Control, Molecular Weight, Marker

Journal: Canadian Journal of Veterinary Research

Article Title: Development of an immunocapture-polymerase chain reaction assay using IgY to detect Mycobacterium avium subsp. paratuberculosis

doi:

Figure Lengend Snippet: Polymerase chain reaction (PCR) results with different concentrations of DNA extracted from ATCC 19698 MAP cells immunocaptured by 40 μL of Beads A incubated at room temperature (RT) for different times; DNA was run undiluted (N) and at dilutions of 1:10 (−1) and 1:100 (−2). The incubation times were as follows: lanes 1 to 3, 15 min; lanes 4 to 6, 30 min; lanes 7 to 9, 60 min. Lane +C — positive-control DNA (ATCC 19698); lane +IC — positive internal-control DNA; lane −C — negative water control; lane M — 1-kb molecular weight marker.

Article Snippet: The concentrations of 3 field and 2 reference strains of MAP [11992, EA4146, 2945, American Type Culture Collection (ATCC) 12258, and ATCC 19698] were adjusted to 2 × 10 7 cells/mL.

Techniques: Polymerase Chain Reaction, Incubation, Positive Control, Control, Molecular Weight, Marker

Journal: Canadian Journal of Veterinary Research

Article Title: Development of an immunocapture-polymerase chain reaction assay using IgY to detect Mycobacterium avium subsp. paratuberculosis

doi:

Figure Lengend Snippet: Results of PCR with the use of 40 μL of Beads A to immunocapture MAP ATCC 19698 spiked in bovine feces (2 × 104 cells/g) incubated at RT for 15 min. Lanes 1 and 2 — DNA diluted 1:10 and 1:100; lanes 3 and 4 — negative-control bovine feces with DNA diluted 1:10 and 1:100; lane 5 — positive-control DNA (ATCC 19698); lane 6 — negative water control; lane M — 1-kb molecular weight marker.

Article Snippet: The concentrations of 3 field and 2 reference strains of MAP [11992, EA4146, 2945, American Type Culture Collection (ATCC) 12258, and ATCC 19698] were adjusted to 2 × 10 7 cells/mL.

Techniques: Incubation, Negative Control, Positive Control, Control, Molecular Weight, Marker

Journal: Canadian Journal of Veterinary Research

Article Title: Development of an immunocapture-polymerase chain reaction assay using IgY to detect Mycobacterium avium subsp. paratuberculosis

doi:

Figure Lengend Snippet: Determination of MagaCell-IgY beads volume required for immunocapture of MAP ATCC 19698 spiked in bovine feces (2 × 104 cells/g) and incubated at RT for 15 min. For each MagaCell-IgY beads volume used, DNA was run undiluted (N) and at dilutions of 1:10 (−1) and 1:100 (−2). The bead volumes were as follows: lanes 1 to 3, 5 μL; lanes 4 to 6, 10 μL; lanes 7 to 9, 15 μL; lanes 10 to 12, 20 μL. Lane M — 1-kb molecular weight marker; lane +C — positive-control DNA (ATCC 19698); lane −C — negative water control.

Article Snippet: The concentrations of 3 field and 2 reference strains of MAP [11992, EA4146, 2945, American Type Culture Collection (ATCC) 12258, and ATCC 19698] were adjusted to 2 × 10 7 cells/mL.

Techniques: Incubation, Molecular Weight, Marker, Positive Control, Control

Journal: Canadian Journal of Veterinary Research

Article Title: Development of an immunocapture-polymerase chain reaction assay using IgY to detect Mycobacterium avium subsp. paratuberculosis

doi:

Figure Lengend Snippet: Results of PCR with the use of 10 μL of MagaCell-IgY beads to immunocapture MAP strain ATCC 19698 spiked in bovine feces at concentrations of 2 × 103 cells/g (lanes 1 to 3), 2 × 104 cells/g (lanes 4 to 6), and 2 × 105 cells/g (lanes 7 to 9). End-point titration was performed with extracted DNA run undiluted (N) and at dilutions of 1:10 (−1) and 1:100 (−2). Lane +C — positive-control DNA (ATCC 19698); lane +IC — positive internal-control DNA; lane −C — negative water control; lane M — 1-kb molecular weight marker.

Article Snippet: The concentrations of 3 field and 2 reference strains of MAP [11992, EA4146, 2945, American Type Culture Collection (ATCC) 12258, and ATCC 19698] were adjusted to 2 × 10 7 cells/mL.

Techniques: Titration, Positive Control, Control, Molecular Weight, Marker