Journal: Scientific Reports

Article Title: Distinguishing core from penumbra by lipid profiles using Mass Spectrometry Imaging in a transgenic mouse model of ischemic stroke

doi: 10.1038/s41598-018-37612-5

Figure Lengend Snippet: A schematic representation of the manual overlays of MALDI-MSI and Nissl stain with marked necrotic border in SCiLS Lab 2016b. The manual overlay was followed by a linear transparency overlay ( a ) of a selected m/z value (in the example m/z = 782.6 ± 0.3). The high intensity of the [PC(34:1) + Na] + perfectly aligns within the necrotic border of the Nissl stain. ( b ) In the next step, the manual overlay was followed by a spatial segmentation pipeline built in the software. Once the segmentation map was created it detected prominent spatial regions ipsilateral, representing core, penumbra and healthy tissue, here at 4 hours after transient middle cerebral artery occlusion.

Article Snippet: All data was acquired on a Bruker RapifleX MALDI TissuetyperTM system operating in reflectron mode (Bruker Daltonik GmbH) with a nominal acceleration potential of ±20 kV.

Techniques: Staining, Software

Journal: Scientific Reports

Article Title: Distinguishing core from penumbra by lipid profiles using Mass Spectrometry Imaging in a transgenic mouse model of ischemic stroke

doi: 10.1038/s41598-018-37612-5

Figure Lengend Snippet: MALDI images showing the distribution of the PIP molecular family and accompanying Nissl stainings. Selected MALDI m/z images acquired at 50-µm spatial resolution showing the distribution of specific lipids m/z = 885.6 [PI(38:4) − H] − , m/z = 965.5 [PIP(38:4) − H] − , and m/z = 1045.5 [PIP 2 (38:4) − H] − in a SHAM WT mouse and at 4, 8 and 24 hours after transient middle cerebral artery occlusion measured in negative ion mode. The last column represents the linear transparency overlays of m/z = 885.6 [PI(38:4) − H] − , m/z = 965.5 [PIP(38:4) − H] − , and m/z = 1045.5 [PIP 2 (38:4) − H] − with Nissl staining at 24 hours after tMCAO.

Article Snippet: All data was acquired on a Bruker RapifleX MALDI TissuetyperTM system operating in reflectron mode (Bruker Daltonik GmbH) with a nominal acceleration potential of ±20 kV.

Techniques: Staining

Journal: Scientific Reports

Article Title: Distinguishing core from penumbra by lipid profiles using Mass Spectrometry Imaging in a transgenic mouse model of ischemic stroke

doi: 10.1038/s41598-018-37612-5

Figure Lengend Snippet: MALDI images showing the distribution of lyso-lipids and accompanying Nissl stainings. Selected MALDI m/z images acquired at 50-µm spatial resolution showing the distribution of lyso-lipids in a SHAM WT mouse and at 4, 8 and 24 hours after transient middle cerebral artery occlusion measured in positive ion mode. Each row of the selected species represents un-washed (top) and washed (bottom) tissue section.

Article Snippet: All data was acquired on a Bruker RapifleX MALDI TissuetyperTM system operating in reflectron mode (Bruker Daltonik GmbH) with a nominal acceleration potential of ±20 kV.

Techniques:

Journal: Scientific Reports

Article Title: Distinguishing core from penumbra by lipid profiles using Mass Spectrometry Imaging in a transgenic mouse model of ischemic stroke

doi: 10.1038/s41598-018-37612-5

Figure Lengend Snippet: MRI, SIMS and MALDI images revealing the distribution of the infarct core in wild-type mice after transient middle cerebral artery occlusion. ( a ) PC4 loadings plot revealing the distribution of co-related species with Na + (positive loadings plot-green) and K + (negative loadings-blue), ( b ) RGB overlay of three PCs (red; PC-3, green; PC4, and blue; PC-4); ( c ) T2-weighted MRI image with delineated infarct border; MALDI image of ( d ) m/z = 756.6 [PC(32:0) + Na] + and ( e ) m/z = 772.6 [PC(32:0) + K] + ; ( f ) K + /Na + ratio, ( g ) choline fragment, ( h ) phosphatidylcholine fragment in positive ion mode and ( i ) palmitic acid (16:0) m/z = 255, in ischemia 24 hours after transient middle middle cerebral artery occlusion.

Article Snippet: All data was acquired on a Bruker RapifleX MALDI TissuetyperTM system operating in reflectron mode (Bruker Daltonik GmbH) with a nominal acceleration potential of ±20 kV.

Techniques:

Journal: Microorganisms

Article Title: Characterization by MALDI-TOF MS and 16S rRNA Gene Sequencing of Aerobic Endospore-Forming Bacteria Isolated from Pharmaceutical Facility in Rio de Janeiro, Brazil

doi: 10.3390/microorganisms12040724

Figure Lengend Snippet: Results of aerobic endospore-forming bacteria (n = 25) identification by VITEK ® MS and MALDI Biotyper ® .

Article Snippet: Proteomic characterization by MALDI-TOF MS was performed using two semi-automated systems: MALDI Biotyper ® (MBT) (Bruker Corporation, Billerica, MA, USA) and VITEK ® MS RUO (bioMérieux, Craponne, France), according to the manufacturers’ instructions.

Techniques: Bacteria, Bioburden Testing, Sterility, Purification

Journal: Microorganisms

Article Title: Characterization by MALDI-TOF MS and 16S rRNA Gene Sequencing of Aerobic Endospore-Forming Bacteria Isolated from Pharmaceutical Facility in Rio de Janeiro, Brazil

doi: 10.3390/microorganisms12040724

Figure Lengend Snippet: Identification by 16S rRNA gene sequencing of the strains (n = 15) not identified by either MALDI TOF/MS system.

Article Snippet: Proteomic characterization by MALDI-TOF MS was performed using two semi-automated systems: MALDI Biotyper ® (MBT) (Bruker Corporation, Billerica, MA, USA) and VITEK ® MS RUO (bioMérieux, Craponne, France), according to the manufacturers’ instructions.

Techniques: Sequencing

Journal: bioRxiv

Article Title: A MALDI-TOF assay identifies nilotinib as an inhibitor of inflammation in acute myeloid leukaemia

doi: 10.1101/2021.03.29.437557

Figure Lengend Snippet: A) Workflow of the MALDI-TOF MS assay: (1) AML cells were pre-treated with a drug for 1h before adding a pro-inflammatory stimulus for 24h. (2) Cells were frozen on dry ice, thawed and (3) washed with 100 mM Tris-HCl buffer at 4°C. (4) Cells were mixed with a matrix (10 mg/mL α-cyano-4-cinnamic acid in 50% acetonitrile, 0.1% trifluoroacetic acid). (5) Cells were analyzed in a rapifleX PharmaPulse MALDI TOF mass spectrometer. (6) In the ionization chamber a laser is used to produce ions in the gas phase. These ions are separated according to their time-of-flight (TOF) in a field-free region. The smaller ions reach the detector first, followed by the bigger ions, according to the m/z ratio. (7) The detector converts the received ions into electrical current which is amplified and digitized in m/z spectra. B) Unsupervised PCA plot of LPS, Pam 2 CSK 4 , Pam 3 CSK 4 and IFN-γ-treated cells showing separation of cells treated with bacterial ligands treated and resting monocytes. C) Loading plot derived from PCA in B) showing that m/z 4964 and 4632 contribute predominantly to the separation of the two clusters in component 1 and component 2. D) Unsupervised heat map of the relative intensities of three biological replicates of THP-1 cells treated with 100 ng/mL LPS, Pam 2 CSK 4 , Pam 3 CSK 4 , 100 U/mL IFN-γ, 1 μg/mL poly(I:C) and poly(A:U) for 24h compared to resting cells E) Box plots of significantly changing intensities between resting and LPS-treated monocytes identified at m/z 4632 and 4964. F) Relative quantitation from three biological replicates of THP-1 cells treated with 100 ng/mL LPS, Pam 2 CSK 4 , Pam 3 CSK 4 and 100 U/mL IFN-γ for 24h compared to resting cells. G) Titration of LPS, Pam 2 CSK 4 and Pam 3 CSK 4 -treated cells from 10 −100 ng/mL of stimulus. H) PCA plot with three dimensions represents resting, LPS-, and 0.5 µM staurosporine-treated cells. Significant differences between two groups were determined by Student’s t-test. The statistical significance of the comparisons with resting is indicated as follows: ns, not significant; ***, P ≤ 0.001; *, P ≤ 0.05. Error bars represent the standard deviation of three biological replicates.

Article Snippet: A RapifleX PharmaPulse MALDI TOF/TOF mass spectrometer (Bruker Daltonics) equipped with a Smartbeam 3D laser was used in positive ion mode.

Techniques: Mass Spectrometry, Amplification, Derivative Assay, Quantitation Assay, Titration, Standard Deviation