Journal: PLoS ONE

Article Title: Identification of Multiple Subsets of Ventral Interneurons and Differential Distribution along the Rostrocaudal Axis of the Developing Spinal Cord

doi: 10.1371/journal.pone.0070325

Figure Lengend Snippet: List of the primary antibodies used in this study.

Article Snippet: Rabbit anti-MafA , 1∶500 , Novus Biological , NB400-137.

Techniques:

Journal: The Journal of Clinical Investigation

Article Title: Sel1L-Hrd1 ER-associated degradation maintains β cell identity via TGF- β signaling

doi: 10.1172/JCI134874

Figure Lengend Snippet: (A) Representative immunofluorescence image showing major islet hormones (insulin, glucagon, and somatostatin; DAPI in blue) in pancreatic sections of WT, Sel1LIns1, and Atg7Ins1 mice (n = 3 mice each). (B–D) Representative immunofluorescence image showing staining of (B) maturation marker MafA with quantitation in C (n = 3 mice, each dot represents an islet), and (D) dedifferentiation marker Aldh1a3 in indicated genotypes. (E) Western blot analysis of Aldh1a3 in primary islets. Values are shown as mean ± SEM. ****P < 0.0001, 1-way ANOVA.

Article Snippet: The following primary antibodies were used: Sel1L (1:200), p62 (Enzo, catalog BML-PW9860; 1:500), insulin (Bio-Rad, catalog 5330-0104G; 1:5000), glucagon (MilliporeSigma, catalog G2654; 1:500), somatostatin (Abcam, catalog ab30788; 1:200), Aldh1a3 (Novus Biologicals, catalog NBP2-15339; 1:100), MafA (Novus Biologicals, catalog NBP1-00121; 1:100), Ucn3 (provided by Mark Huising (University of California, Davis, Davis, CA, USA); 1:1000), Pdx1 (Cell Signaling Technology, catalog 5679; 1:100) Smad2/3 (Cell Signaling Technology, catalog 8685; 1:1000), proinsulin (DSHB, catalog GS-9A8; 1:100), E-Cadherin (BD 610181; 1:500), and BiP (Abcam, catalog 21685; 1:500).

Techniques: Immunofluorescence, Staining, Marker, Quantitation Assay, Western Blot