Journal: eLife

Article Title: Distinct and diverse chromatin proteomes of ageing mouse organs reveal protein signatures that correlate with physiological functions

doi: 10.7554/eLife.73524

Figure Lengend Snippet: ( A ) Volcano plots of differentially regulated proteins across six organs (brain, heart, kidney, lung, liver, and spleen) between the early (3 months) and the late (15 months) adult mouse lifespan. Expressed protein significantly changed highlighted in red and green show respectively downregulation and upregulation during the mouse lifespan. Specific enrichments for each protein were calculated by Rack test. Adjusted q-values were calculated to correct for multiple testing (-log10 q-value <0.1 cutoff) ( B ) Co-expression cluster profile, extrapolated from the hierarchical clustering heatmap, shows the ‘unique differentially expressed protein’ trend changed over time in each organ.( C ) Metadata analysis reported the quantitative changed expression profile of human ageing biomarker candidates obtained from the ‘Human Ageing Genomic Resources’ and ‘GenAge machine learning databank’.

Article Snippet: Next, we queried the ‘Human Ageing Genomic Resources’ and ‘GenAge machine learning databank’ using our complete list of ‘unique regulated proteins’ that are not yet assigned to chromatin or nuclear environment to demonstrate the ability of our mouse organ proteomics approach to detect known human ageing biomarkers ( ; ; ).

Techniques: Expressing, Biomarker Discovery