mabs Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Millipore monoclonal anti flag m2 antibody
    Monoclonal Anti Flag M2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 37942 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti flag m2 antibody/product/Millipore
    Average 97 stars, based on 37942 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti flag m2 antibody - by Bioz Stars, 2021-02
    97/100 stars
      Buy from Supplier

    99
    Millipore β actin
    CCP6 or CCP1 deficiency promotes cell reprogramming. a Ccp1 - or Ccp6 - deficient MEFs were lyzed for immunoblotting. <t>β-actin</t> was used as a loading control. b WT or CCPs-deficient MEFs were infected by OSKM factors containing retrovirus and cultured in ESC media for 3 weeks. Alkaline phosphatase (AP)-positive colony numbers per 10 4 cells were calculated and shown as means ± S.D. **, P
    β Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 67284 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β actin/product/Millipore
    Average 99 stars, based on 67284 article reviews
    Price from $9.99 to $1999.99
    β actin - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    99
    Millipore monoclonal anti beta actin antibody
    CCP6 or CCP1 deficiency promotes cell reprogramming. a Ccp1 - or Ccp6 - deficient MEFs were lyzed for immunoblotting. <t>β-actin</t> was used as a loading control. b WT or CCPs-deficient MEFs were infected by OSKM factors containing retrovirus and cultured in ESC media for 3 weeks. Alkaline phosphatase (AP)-positive colony numbers per 10 4 cells were calculated and shown as means ± S.D. **, P
    Monoclonal Anti Beta Actin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 34567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti beta actin antibody/product/Millipore
    Average 99 stars, based on 34567 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti beta actin antibody - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    99
    Millipore monoclonal anti beta tubulin antibody
    CCP6 or CCP1 deficiency promotes cell reprogramming. a Ccp1 - or Ccp6 - deficient MEFs were lyzed for immunoblotting. <t>β-actin</t> was used as a loading control. b WT or CCPs-deficient MEFs were infected by OSKM factors containing retrovirus and cultured in ESC media for 3 weeks. Alkaline phosphatase (AP)-positive colony numbers per 10 4 cells were calculated and shown as means ± S.D. **, P
    Monoclonal Anti Beta Tubulin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4638 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti beta tubulin antibody/product/Millipore
    Average 99 stars, based on 4638 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti beta tubulin antibody - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    99
    Millipore anti β actin
    Pellino2 ubiquitinates IRAK1 and suppress the inhibitory effects of IRAK1 on NLRP3. a Immunoblot analysis of myc and IRAK1 in lysates (Input) and immunoprecipitated (IP) myc samples from HEK293T cells transfected with myc-tagged Pellino2 and untagged IRAK1. b Immunoblot analysis of HA, IRAK1 and FLAG in lysates (Input) and IP IRAK1 samples from HEK293T cells transfected with FLAG-tagged Pellino2, FLAG-tagged Pellino2 RING mutant, untagged IRAK1, and HA-Ubiquitin. c, d Immunoblot analysis of Ubiquitin (short exposure (SE) or long exposure (LE)) and IRAK1 in lysates (Input) and IP IRAK1 samples or IRAK1 and ubq in TUBE-Ubq elution and cell lysates ( d ) from WT and Peli2 −/− BMDMs treated with 100 ng/ml of LPS for the indicated times. e Immunoblot analysis of NLRP3 and IRAK1 in lysates (Input) and IP IRAK1 samples from WT and Peli2 −/− BMDMs treated with 100 ng/ml LPS for the indicated times. f ELISA of IL-1β (left panel) and TNF (right panel) in medium from primary WT and Irak1 −/− BMDMs treated with 100 ng/ml LPS for 3 h and then with 2.5 mM ATP or 5 mM Nigericin for 1 h. UT, untreated. g Immunoblot analysis of IL-1β and Caspase-1 in medium (Sup) and lysates of WT and Irak1 −/− BMDMs stimulated with 100 ng/ml LPS for 3 h and 2.5 mM ATP for 1 h. h Immunoblot analysis of HA, NLRP3, and IRAK1 in lysates (Input) and IP NLRP3 samples from HEK293T cells transfected with V5-tagged NLRP3, untagged IRAK1, untagged kinase dead IRAK1 (IRAK1-KD), and HA-ubiquitin. <t>β-Actin</t> was used as loading controls. i Immunoblot analysis of Ubiquitin, NLRP3, and IRAK1 in lysates (Input) and IP NLRP3 samples from immortalized WT and Irak1 −/− BMDMs treated with 100 ng/ml LPS for the indicated times. j Immortalized WT and Irak1 −/− BMDMs were infected with MSCV as control (Ctrl) or with MSCV containing an expression construct encoding myc-tagged murine Pellino2 (Peli2), Pellino2 RING mutant (RING) or Pellino2 FHA mutant (FHA). Immunoblot analysis of myc and NLRP3 in lysates (Input) and IP myc samples from virus-infected BMDMs treated with 100 ng/ml LPS for indicated times. * p
    Anti β Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 27592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti β actin/product/Millipore
    Average 99 stars, based on 27592 article reviews
    Price from $9.99 to $1999.99
    anti β actin - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc gapdh
    Pellino2 ubiquitinates IRAK1 and suppress the inhibitory effects of IRAK1 on NLRP3. a Immunoblot analysis of myc and IRAK1 in lysates (Input) and immunoprecipitated (IP) myc samples from HEK293T cells transfected with myc-tagged Pellino2 and untagged IRAK1. b Immunoblot analysis of HA, IRAK1 and FLAG in lysates (Input) and IP IRAK1 samples from HEK293T cells transfected with FLAG-tagged Pellino2, FLAG-tagged Pellino2 RING mutant, untagged IRAK1, and HA-Ubiquitin. c, d Immunoblot analysis of Ubiquitin (short exposure (SE) or long exposure (LE)) and IRAK1 in lysates (Input) and IP IRAK1 samples or IRAK1 and ubq in TUBE-Ubq elution and cell lysates ( d ) from WT and Peli2 −/− BMDMs treated with 100 ng/ml of LPS for the indicated times. e Immunoblot analysis of NLRP3 and IRAK1 in lysates (Input) and IP IRAK1 samples from WT and Peli2 −/− BMDMs treated with 100 ng/ml LPS for the indicated times. f ELISA of IL-1β (left panel) and TNF (right panel) in medium from primary WT and Irak1 −/− BMDMs treated with 100 ng/ml LPS for 3 h and then with 2.5 mM ATP or 5 mM Nigericin for 1 h. UT, untreated. g Immunoblot analysis of IL-1β and Caspase-1 in medium (Sup) and lysates of WT and Irak1 −/− BMDMs stimulated with 100 ng/ml LPS for 3 h and 2.5 mM ATP for 1 h. h Immunoblot analysis of HA, NLRP3, and IRAK1 in lysates (Input) and IP NLRP3 samples from HEK293T cells transfected with V5-tagged NLRP3, untagged IRAK1, untagged kinase dead IRAK1 (IRAK1-KD), and HA-ubiquitin. <t>β-Actin</t> was used as loading controls. i Immunoblot analysis of Ubiquitin, NLRP3, and IRAK1 in lysates (Input) and IP NLRP3 samples from immortalized WT and Irak1 −/− BMDMs treated with 100 ng/ml LPS for the indicated times. j Immortalized WT and Irak1 −/− BMDMs were infected with MSCV as control (Ctrl) or with MSCV containing an expression construct encoding myc-tagged murine Pellino2 (Peli2), Pellino2 RING mutant (RING) or Pellino2 FHA mutant (FHA). Immunoblot analysis of myc and NLRP3 in lysates (Input) and IP myc samples from virus-infected BMDMs treated with 100 ng/ml LPS for indicated times. * p
    Gapdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 30834 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gapdh/product/Cell Signaling Technology Inc
    Average 99 stars, based on 30834 article reviews
    Price from $9.99 to $1999.99
    gapdh - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc cleaved caspase 3
    REV-ERB agonist SR9011 inhibit autophagy a–b, SR90011 treatment reduces the number of autophagosomes both in MCF7 and T47D, n= biological independent samples MCF7 20µM 24h n=9 (mock), n=4 (SR9011) ** P =0.0056, T47D 48h 20µM n=5 (mock), n=4 (SR9011) ** P =0.0079; c–d, SR9011 induces accumulation of p62 as shown by immunofluorescence both in MCF7 and T47D n= biological independent samples 48h MCF7 p62 n=3 (mock), n=4 (SR9011) * P =0.0286; 48h T47D n=5 (mock, SR9011) ** P =0.004; e, Accumulation of p62 is confirmed by immunoblot (48h, 20µM A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved <t>Caspase</t> 3 and TUNEL assay (n= biological independent samples n=4 mock 48h, n=5 SR9011 p62, n=3 48h SR9011; n=6 (mock, SR9011 72h p62), n=10 (mock 72h), n=8 (SR9011 72h) A375 20µM, Cl. Casp. 3 48h * P =0.0286; Cl. Casp. 3 72h **** P
    Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 41696 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 3/product/Cell Signaling Technology Inc
    Average 99 stars, based on 41696 article reviews
    Price from $9.99 to $1999.99
    cleaved caspase 3 - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc erk1 2
    REV-ERB agonist SR9011 inhibit autophagy a–b, SR90011 treatment reduces the number of autophagosomes both in MCF7 and T47D, n= biological independent samples MCF7 20µM 24h n=9 (mock), n=4 (SR9011) ** P =0.0056, T47D 48h 20µM n=5 (mock), n=4 (SR9011) ** P =0.0079; c–d, SR9011 induces accumulation of p62 as shown by immunofluorescence both in MCF7 and T47D n= biological independent samples 48h MCF7 p62 n=3 (mock), n=4 (SR9011) * P =0.0286; 48h T47D n=5 (mock, SR9011) ** P =0.004; e, Accumulation of p62 is confirmed by immunoblot (48h, 20µM A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved <t>Caspase</t> 3 and TUNEL assay (n= biological independent samples n=4 mock 48h, n=5 SR9011 p62, n=3 48h SR9011; n=6 (mock, SR9011 72h p62), n=10 (mock 72h), n=8 (SR9011 72h) A375 20µM, Cl. Casp. 3 48h * P =0.0286; Cl. Casp. 3 72h **** P
    Erk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 22641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erk1 2/product/Cell Signaling Technology Inc
    Average 99 stars, based on 22641 article reviews
    Price from $9.99 to $1999.99
    erk1 2 - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    99
    Millipore actin
    REV-ERB agonist SR9011 inhibit autophagy a–b, SR90011 treatment reduces the number of autophagosomes both in MCF7 and T47D, n= biological independent samples MCF7 20µM 24h n=9 (mock), n=4 (SR9011) ** P =0.0056, T47D 48h 20µM n=5 (mock), n=4 (SR9011) ** P =0.0079; c–d, SR9011 induces accumulation of p62 as shown by immunofluorescence both in MCF7 and T47D n= biological independent samples 48h MCF7 p62 n=3 (mock), n=4 (SR9011) * P =0.0286; 48h T47D n=5 (mock, SR9011) ** P =0.004; e, Accumulation of p62 is confirmed by immunoblot (48h, 20µM A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved <t>Caspase</t> 3 and TUNEL assay (n= biological independent samples n=4 mock 48h, n=5 SR9011 p62, n=3 48h SR9011; n=6 (mock, SR9011 72h p62), n=10 (mock 72h), n=8 (SR9011 72h) A375 20µM, Cl. Casp. 3 48h * P =0.0286; Cl. Casp. 3 72h **** P
    Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 16414 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/actin/product/Millipore
    Average 99 stars, based on 16414 article reviews
    Price from $9.99 to $1999.99
    actin - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    99
    Millipore mouse monoclonal antibody
    REV-ERB agonist SR9011 inhibit autophagy a–b, SR90011 treatment reduces the number of autophagosomes both in MCF7 and T47D, n= biological independent samples MCF7 20µM 24h n=9 (mock), n=4 (SR9011) ** P =0.0056, T47D 48h 20µM n=5 (mock), n=4 (SR9011) ** P =0.0079; c–d, SR9011 induces accumulation of p62 as shown by immunofluorescence both in MCF7 and T47D n= biological independent samples 48h MCF7 p62 n=3 (mock), n=4 (SR9011) * P =0.0286; 48h T47D n=5 (mock, SR9011) ** P =0.004; e, Accumulation of p62 is confirmed by immunoblot (48h, 20µM A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved <t>Caspase</t> 3 and TUNEL assay (n= biological independent samples n=4 mock 48h, n=5 SR9011 p62, n=3 48h SR9011; n=6 (mock, SR9011 72h p62), n=10 (mock 72h), n=8 (SR9011 72h) A375 20µM, Cl. Casp. 3 48h * P =0.0286; Cl. Casp. 3 72h **** P
    Mouse Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal antibody/product/Millipore
    Average 99 stars, based on 11460 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal antibody - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc β actin
    REV-ERB agonist SR9011 inhibit autophagy a–b, SR90011 treatment reduces the number of autophagosomes both in MCF7 and T47D, n= biological independent samples MCF7 20µM 24h n=9 (mock), n=4 (SR9011) ** P =0.0056, T47D 48h 20µM n=5 (mock), n=4 (SR9011) ** P =0.0079; c–d, SR9011 induces accumulation of p62 as shown by immunofluorescence both in MCF7 and T47D n= biological independent samples 48h MCF7 p62 n=3 (mock), n=4 (SR9011) * P =0.0286; 48h T47D n=5 (mock, SR9011) ** P =0.004; e, Accumulation of p62 is confirmed by immunoblot (48h, 20µM A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved <t>Caspase</t> 3 and TUNEL assay (n= biological independent samples n=4 mock 48h, n=5 SR9011 p62, n=3 48h SR9011; n=6 (mock, SR9011 72h p62), n=10 (mock 72h), n=8 (SR9011 72h) A375 20µM, Cl. Casp. 3 48h * P =0.0286; Cl. Casp. 3 72h **** P
    β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 38293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β actin/product/Cell Signaling Technology Inc
    Average 99 stars, based on 38293 article reviews
    Price from $9.99 to $1999.99
    β actin - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher anti cd3
    IL-31R signaling did not influence the differentiation of CD4 + T cell sub-populations. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with <t>anti-CD3/anti-CD28</t> under Th1-, Th2- or Th17- polarizing conditions. Cells were assayed for the transcription factors T-bet, GATA-3, ROR-γt and cytokines IFN-γ, IL-4, IL-17 by flow cytometry. No difference on the differentiation of Th1(A), Th2 (B) and Th17 (C) was found between WT ( n =4) and IL-31RA KO mice ( n =4). All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated three times. P, polarization; NP, non-polarization.
    Anti Cd3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd3/product/Thermo Fisher
    Average 99 stars, based on 12407 article reviews
    Price from $9.99 to $1999.99
    anti cd3 - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    97
    Becton Dickinson cd11b
    IL-31R signaling did not influence the differentiation of CD4 + T cell sub-populations. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with <t>anti-CD3/anti-CD28</t> under Th1-, Th2- or Th17- polarizing conditions. Cells were assayed for the transcription factors T-bet, GATA-3, ROR-γt and cytokines IFN-γ, IL-4, IL-17 by flow cytometry. No difference on the differentiation of Th1(A), Th2 (B) and Th17 (C) was found between WT ( n =4) and IL-31RA KO mice ( n =4). All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated three times. P, polarization; NP, non-polarization.
    Cd11b, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 97/100, based on 13372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd11b/product/Becton Dickinson
    Average 97 stars, based on 13372 article reviews
    Price from $9.99 to $1999.99
    cd11b - by Bioz Stars, 2021-02
    97/100 stars
      Buy from Supplier

    97
    Becton Dickinson anti cd28
    IL-31R signaling did not influence the differentiation of CD4 + T cell sub-populations. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with <t>anti-CD3/anti-CD28</t> under Th1-, Th2- or Th17- polarizing conditions. Cells were assayed for the transcription factors T-bet, GATA-3, ROR-γt and cytokines IFN-γ, IL-4, IL-17 by flow cytometry. No difference on the differentiation of Th1(A), Th2 (B) and Th17 (C) was found between WT ( n =4) and IL-31RA KO mice ( n =4). All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated three times. P, polarization; NP, non-polarization.
    Anti Cd28, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 97/100, based on 9921 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd28/product/Becton Dickinson
    Average 97 stars, based on 9921 article reviews
    Price from $9.99 to $1999.99
    anti cd28 - by Bioz Stars, 2021-02
    97/100 stars
      Buy from Supplier

    99
    Thermo Fisher cd8a
    IL-31R signaling did not influence the differentiation of CD4 + T cell sub-populations. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with <t>anti-CD3/anti-CD28</t> under Th1-, Th2- or Th17- polarizing conditions. Cells were assayed for the transcription factors T-bet, GATA-3, ROR-γt and cytokines IFN-γ, IL-4, IL-17 by flow cytometry. No difference on the differentiation of Th1(A), Th2 (B) and Th17 (C) was found between WT ( n =4) and IL-31RA KO mice ( n =4). All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated three times. P, polarization; NP, non-polarization.
    Cd8a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd8a/product/Thermo Fisher
    Average 99 stars, based on 1237 article reviews
    Price from $9.99 to $1999.99
    cd8a - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    97
    Becton Dickinson e cadherin
    The effects of daily different dwelling times on cellular protein expression and cell survival. Expression of <t>E-cadherin</t> in mesothelial cells (MCs) was assessed on day 3, and cell counts were determined on days 3 and 6. A) The expression of E-cadherin, exhibited significant down-regulation with increasing dwell time (N = 4). B) The cell count decreased with a longer daily dwell time, where the maximal decrease was evident in the 24 hour group. On day 3, the 24 hour group demonstrated a significantly lower cell count compared to the phosphate-buffered saline (PBS) group. On day 6, the 16 and 24 hour groups demonstrated a significantly lower cell count compared to the PBS group. Additionally, the 24 hour group also demonstrated a significantly lower cell count compared to the transforming growth factor-β1 (TGF-β1) group (N = 4) (* P
    E Cadherin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 97/100, based on 9399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e cadherin/product/Becton Dickinson
    Average 97 stars, based on 9399 article reviews
    Price from $9.99 to $1999.99
    e cadherin - by Bioz Stars, 2021-02
    97/100 stars
      Buy from Supplier

    99
    Millipore anti alpha tubulin antibody mouse monoclonal
    The effects of daily different dwelling times on cellular protein expression and cell survival. Expression of <t>E-cadherin</t> in mesothelial cells (MCs) was assessed on day 3, and cell counts were determined on days 3 and 6. A) The expression of E-cadherin, exhibited significant down-regulation with increasing dwell time (N = 4). B) The cell count decreased with a longer daily dwell time, where the maximal decrease was evident in the 24 hour group. On day 3, the 24 hour group demonstrated a significantly lower cell count compared to the phosphate-buffered saline (PBS) group. On day 6, the 16 and 24 hour groups demonstrated a significantly lower cell count compared to the PBS group. Additionally, the 24 hour group also demonstrated a significantly lower cell count compared to the transforming growth factor-β1 (TGF-β1) group (N = 4) (* P
    Anti Alpha Tubulin Antibody Mouse Monoclonal, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4746 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti alpha tubulin antibody mouse monoclonal/product/Millipore
    Average 99 stars, based on 4746 article reviews
    Price from $9.99 to $1999.99
    anti alpha tubulin antibody mouse monoclonal - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    99
    Millipore igg
    The effects of daily different dwelling times on cellular protein expression and cell survival. Expression of <t>E-cadherin</t> in mesothelial cells (MCs) was assessed on day 3, and cell counts were determined on days 3 and 6. A) The expression of E-cadherin, exhibited significant down-regulation with increasing dwell time (N = 4). B) The cell count decreased with a longer daily dwell time, where the maximal decrease was evident in the 24 hour group. On day 3, the 24 hour group demonstrated a significantly lower cell count compared to the phosphate-buffered saline (PBS) group. On day 6, the 16 and 24 hour groups demonstrated a significantly lower cell count compared to the PBS group. Additionally, the 24 hour group also demonstrated a significantly lower cell count compared to the transforming growth factor-β1 (TGF-β1) group (N = 4) (* P
    Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg/product/Millipore
    Average 99 stars, based on 10101 article reviews
    Price from $9.99 to $1999.99
    igg - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher cd11b
    The effects of daily different dwelling times on cellular protein expression and cell survival. Expression of <t>E-cadherin</t> in mesothelial cells (MCs) was assessed on day 3, and cell counts were determined on days 3 and 6. A) The expression of E-cadherin, exhibited significant down-regulation with increasing dwell time (N = 4). B) The cell count decreased with a longer daily dwell time, where the maximal decrease was evident in the 24 hour group. On day 3, the 24 hour group demonstrated a significantly lower cell count compared to the phosphate-buffered saline (PBS) group. On day 6, the 16 and 24 hour groups demonstrated a significantly lower cell count compared to the PBS group. Additionally, the 24 hour group also demonstrated a significantly lower cell count compared to the transforming growth factor-β1 (TGF-β1) group (N = 4) (* P
    Cd11b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 10005 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd11b/product/Thermo Fisher
    Average 92 stars, based on 10005 article reviews
    Price from $9.99 to $1999.99
    cd11b - by Bioz Stars, 2021-02
    92/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc akt
    The effects of daily different dwelling times on cellular protein expression and cell survival. Expression of <t>E-cadherin</t> in mesothelial cells (MCs) was assessed on day 3, and cell counts were determined on days 3 and 6. A) The expression of E-cadherin, exhibited significant down-regulation with increasing dwell time (N = 4). B) The cell count decreased with a longer daily dwell time, where the maximal decrease was evident in the 24 hour group. On day 3, the 24 hour group demonstrated a significantly lower cell count compared to the phosphate-buffered saline (PBS) group. On day 6, the 16 and 24 hour groups demonstrated a significantly lower cell count compared to the PBS group. Additionally, the 24 hour group also demonstrated a significantly lower cell count compared to the transforming growth factor-β1 (TGF-β1) group (N = 4) (* P
    Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 48620 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/akt/product/Cell Signaling Technology Inc
    Average 99 stars, based on 48620 article reviews
    Price from $9.99 to $1999.99
    akt - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    94
    Abcam mouse monoclonal
    The effects of daily different dwelling times on cellular protein expression and cell survival. Expression of <t>E-cadherin</t> in mesothelial cells (MCs) was assessed on day 3, and cell counts were determined on days 3 and 6. A) The expression of E-cadherin, exhibited significant down-regulation with increasing dwell time (N = 4). B) The cell count decreased with a longer daily dwell time, where the maximal decrease was evident in the 24 hour group. On day 3, the 24 hour group demonstrated a significantly lower cell count compared to the phosphate-buffered saline (PBS) group. On day 6, the 16 and 24 hour groups demonstrated a significantly lower cell count compared to the PBS group. Additionally, the 24 hour group also demonstrated a significantly lower cell count compared to the transforming growth factor-β1 (TGF-β1) group (N = 4) (* P
    Mouse Monoclonal, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 917 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal/product/Abcam
    Average 94 stars, based on 917 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal - by Bioz Stars, 2021-02
    94/100 stars
      Buy from Supplier

    N/A
    β galactosidase antibody Mouse IgG1e3 isotype control
      Buy from Supplier

    N/A
    β galactosidase antibody Mouse IgG2a isotype control
      Buy from Supplier

    N/A
    Phosphotyrosine Molecular Weight Marker Contains the following phosphotyrosine modified proteins Galactosidase 114 kDa Phosphorylase A 94 kDa Conalbumin 78 kDa BSA 67 kDa Ovalbumin 47 kDa Carbonic anhydrase 30 kDa
      Buy from Supplier

    N/A
    Phosphoserine Set of 6 MAbs is a mouse monoclonal antibody to apoptosis and signal transduction reagent Host Note Mouse Conjugation Note Unconjugated Application Note ELISA WB IP
      Buy from Supplier

    Image Search Results


    CCP6 or CCP1 deficiency promotes cell reprogramming. a Ccp1 - or Ccp6 - deficient MEFs were lyzed for immunoblotting. β-actin was used as a loading control. b WT or CCPs-deficient MEFs were infected by OSKM factors containing retrovirus and cultured in ESC media for 3 weeks. Alkaline phosphatase (AP)-positive colony numbers per 10 4 cells were calculated and shown as means ± S.D. **, P

    Journal: Nature Communications

    Article Title: Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice

    doi: 10.1038/s41467-018-03008-2

    Figure Lengend Snippet: CCP6 or CCP1 deficiency promotes cell reprogramming. a Ccp1 - or Ccp6 - deficient MEFs were lyzed for immunoblotting. β-actin was used as a loading control. b WT or CCPs-deficient MEFs were infected by OSKM factors containing retrovirus and cultured in ESC media for 3 weeks. Alkaline phosphatase (AP)-positive colony numbers per 10 4 cells were calculated and shown as means ± S.D. **, P

    Article Snippet: Antibodies against Flag-tag (M2, F3165), β-actin (A-5316), His-tag (H1029), glutamylated tubulin (B3), and GFP-tag (G-1544) were from Sigma-Aldrich (St. Louis, USA).

    Techniques: Infection, Cell Culture

    Pellino2 ubiquitinates IRAK1 and suppress the inhibitory effects of IRAK1 on NLRP3. a Immunoblot analysis of myc and IRAK1 in lysates (Input) and immunoprecipitated (IP) myc samples from HEK293T cells transfected with myc-tagged Pellino2 and untagged IRAK1. b Immunoblot analysis of HA, IRAK1 and FLAG in lysates (Input) and IP IRAK1 samples from HEK293T cells transfected with FLAG-tagged Pellino2, FLAG-tagged Pellino2 RING mutant, untagged IRAK1, and HA-Ubiquitin. c, d Immunoblot analysis of Ubiquitin (short exposure (SE) or long exposure (LE)) and IRAK1 in lysates (Input) and IP IRAK1 samples or IRAK1 and ubq in TUBE-Ubq elution and cell lysates ( d ) from WT and Peli2 −/− BMDMs treated with 100 ng/ml of LPS for the indicated times. e Immunoblot analysis of NLRP3 and IRAK1 in lysates (Input) and IP IRAK1 samples from WT and Peli2 −/− BMDMs treated with 100 ng/ml LPS for the indicated times. f ELISA of IL-1β (left panel) and TNF (right panel) in medium from primary WT and Irak1 −/− BMDMs treated with 100 ng/ml LPS for 3 h and then with 2.5 mM ATP or 5 mM Nigericin for 1 h. UT, untreated. g Immunoblot analysis of IL-1β and Caspase-1 in medium (Sup) and lysates of WT and Irak1 −/− BMDMs stimulated with 100 ng/ml LPS for 3 h and 2.5 mM ATP for 1 h. h Immunoblot analysis of HA, NLRP3, and IRAK1 in lysates (Input) and IP NLRP3 samples from HEK293T cells transfected with V5-tagged NLRP3, untagged IRAK1, untagged kinase dead IRAK1 (IRAK1-KD), and HA-ubiquitin. β-Actin was used as loading controls. i Immunoblot analysis of Ubiquitin, NLRP3, and IRAK1 in lysates (Input) and IP NLRP3 samples from immortalized WT and Irak1 −/− BMDMs treated with 100 ng/ml LPS for the indicated times. j Immortalized WT and Irak1 −/− BMDMs were infected with MSCV as control (Ctrl) or with MSCV containing an expression construct encoding myc-tagged murine Pellino2 (Peli2), Pellino2 RING mutant (RING) or Pellino2 FHA mutant (FHA). Immunoblot analysis of myc and NLRP3 in lysates (Input) and IP myc samples from virus-infected BMDMs treated with 100 ng/ml LPS for indicated times. * p

    Journal: Nature Communications

    Article Title: The E3 ubiquitin ligase Pellino2 mediates priming of the NLRP3 inflammasome

    doi: 10.1038/s41467-018-03669-z

    Figure Lengend Snippet: Pellino2 ubiquitinates IRAK1 and suppress the inhibitory effects of IRAK1 on NLRP3. a Immunoblot analysis of myc and IRAK1 in lysates (Input) and immunoprecipitated (IP) myc samples from HEK293T cells transfected with myc-tagged Pellino2 and untagged IRAK1. b Immunoblot analysis of HA, IRAK1 and FLAG in lysates (Input) and IP IRAK1 samples from HEK293T cells transfected with FLAG-tagged Pellino2, FLAG-tagged Pellino2 RING mutant, untagged IRAK1, and HA-Ubiquitin. c, d Immunoblot analysis of Ubiquitin (short exposure (SE) or long exposure (LE)) and IRAK1 in lysates (Input) and IP IRAK1 samples or IRAK1 and ubq in TUBE-Ubq elution and cell lysates ( d ) from WT and Peli2 −/− BMDMs treated with 100 ng/ml of LPS for the indicated times. e Immunoblot analysis of NLRP3 and IRAK1 in lysates (Input) and IP IRAK1 samples from WT and Peli2 −/− BMDMs treated with 100 ng/ml LPS for the indicated times. f ELISA of IL-1β (left panel) and TNF (right panel) in medium from primary WT and Irak1 −/− BMDMs treated with 100 ng/ml LPS for 3 h and then with 2.5 mM ATP or 5 mM Nigericin for 1 h. UT, untreated. g Immunoblot analysis of IL-1β and Caspase-1 in medium (Sup) and lysates of WT and Irak1 −/− BMDMs stimulated with 100 ng/ml LPS for 3 h and 2.5 mM ATP for 1 h. h Immunoblot analysis of HA, NLRP3, and IRAK1 in lysates (Input) and IP NLRP3 samples from HEK293T cells transfected with V5-tagged NLRP3, untagged IRAK1, untagged kinase dead IRAK1 (IRAK1-KD), and HA-ubiquitin. β-Actin was used as loading controls. i Immunoblot analysis of Ubiquitin, NLRP3, and IRAK1 in lysates (Input) and IP NLRP3 samples from immortalized WT and Irak1 −/− BMDMs treated with 100 ng/ml LPS for the indicated times. j Immortalized WT and Irak1 −/− BMDMs were infected with MSCV as control (Ctrl) or with MSCV containing an expression construct encoding myc-tagged murine Pellino2 (Peli2), Pellino2 RING mutant (RING) or Pellino2 FHA mutant (FHA). Immunoblot analysis of myc and NLRP3 in lysates (Input) and IP myc samples from virus-infected BMDMs treated with 100 ng/ml LPS for indicated times. * p

    Article Snippet: Anti-p-ERK (9101), anti-ERK (9102), anti-p-IκBα (9246), anti-pIKK (14938), anti-p65 (3033) anti-p-P38 (9211), anti-P38 (9212), anti-p-Jnk (9251), anti-Jnk (9252), anti-myc (2276), anti-IRAK1 (4504), anti-IRAK4 (4363), and anti-human caspase 1 p20 (4199) were from CellSignaling; anti-mouse caspase1 p20 (AG-20B-0042-C100) and anti-mouse NLRP3 (AG-20B-0014-C100) was from adipogen; anti-mouse IL1β (AF-401-NA) was from R & D; anti-IκB-α (C-21; sc-371), anti-Ub (P4D1; sc-8017), and anti-ASC (sc-514414) was from Santa Cruz; anti-HA (16B12; MMS-101P) was from Covance; anti-K63 ubiquitin (HWA4C4; BML-PW0600) was from Enzo life science; anti-K48 ubiquitin (Apu2; 05-1307) was from Millipore; anti-flag (F3165) and anti-β-actin (AC-15; A 1978) were from Sigma; anti-mouse IRDyeTM 680 (926-68070) and anti-rabbit IRDyeTM 800 (926-32211) were from LI-COR Biosciences; anti-mouse-HRP and anti-rabbit-HRP were from Promega.

    Techniques: Immunoprecipitation, Transfection, Mutagenesis, Enzyme-linked Immunosorbent Assay, Infection, Expressing, Construct

    Pellino2 mediates NLRP3-dependent oligomerization of ASC. a Immunofluorescence staining of ASC in WT and Peli2 −/− BMDMs that were left untreated (UT) or treated with 100 ng/ml LPS for 3 h and further stimulated with ATP for 30 min. ASC specks were detected by immunostaining using anti-ASC antibody and anti-rabbit Alexa Fluor 568 (red) and cells were counter stained with nuclei-staining DAPI. The histogram quantitates the percentage of cells that exhibit ASC speck formation. (scale bar = 100 μm). b, c Immunoblot analysis of ASC in chemically cross-linked NP-40 insoluble fractions and in NP-40 soluble fractions from cell lysates of WT and Peli2 −/− BMDMs stimulated with 100 ng/ml LPS for 3 h with or without further treatment with b 2.5 mM ATP, or c 5 mM Nigericin for 30 min. β-Actin was used as loading controls. d WT and Peli2 −/− BMDMs were infected with MSCV as control (Ctrl) or with MSCV containing an expression construct encoding myc-tagged murine Pellino2 (Peli2), Pellino2 RING mutant (RING), or Pellino2 FHA mutant (FHA). Immunoblot analysis of ASC in chemically cross-linked NP-40 insoluble fractions and in NP-40 soluble fractions from cell lysates of MSCV-infected cells treated with 100 ng/ml LPS for 3 h followed by 2.5 mM ATP for 30 min. The expression of the Pellino2 constructs was measured by immunoblotting with an anti-myc antibody. ** p

    Journal: Nature Communications

    Article Title: The E3 ubiquitin ligase Pellino2 mediates priming of the NLRP3 inflammasome

    doi: 10.1038/s41467-018-03669-z

    Figure Lengend Snippet: Pellino2 mediates NLRP3-dependent oligomerization of ASC. a Immunofluorescence staining of ASC in WT and Peli2 −/− BMDMs that were left untreated (UT) or treated with 100 ng/ml LPS for 3 h and further stimulated with ATP for 30 min. ASC specks were detected by immunostaining using anti-ASC antibody and anti-rabbit Alexa Fluor 568 (red) and cells were counter stained with nuclei-staining DAPI. The histogram quantitates the percentage of cells that exhibit ASC speck formation. (scale bar = 100 μm). b, c Immunoblot analysis of ASC in chemically cross-linked NP-40 insoluble fractions and in NP-40 soluble fractions from cell lysates of WT and Peli2 −/− BMDMs stimulated with 100 ng/ml LPS for 3 h with or without further treatment with b 2.5 mM ATP, or c 5 mM Nigericin for 30 min. β-Actin was used as loading controls. d WT and Peli2 −/− BMDMs were infected with MSCV as control (Ctrl) or with MSCV containing an expression construct encoding myc-tagged murine Pellino2 (Peli2), Pellino2 RING mutant (RING), or Pellino2 FHA mutant (FHA). Immunoblot analysis of ASC in chemically cross-linked NP-40 insoluble fractions and in NP-40 soluble fractions from cell lysates of MSCV-infected cells treated with 100 ng/ml LPS for 3 h followed by 2.5 mM ATP for 30 min. The expression of the Pellino2 constructs was measured by immunoblotting with an anti-myc antibody. ** p

    Article Snippet: Anti-p-ERK (9101), anti-ERK (9102), anti-p-IκBα (9246), anti-pIKK (14938), anti-p65 (3033) anti-p-P38 (9211), anti-P38 (9212), anti-p-Jnk (9251), anti-Jnk (9252), anti-myc (2276), anti-IRAK1 (4504), anti-IRAK4 (4363), and anti-human caspase 1 p20 (4199) were from CellSignaling; anti-mouse caspase1 p20 (AG-20B-0042-C100) and anti-mouse NLRP3 (AG-20B-0014-C100) was from adipogen; anti-mouse IL1β (AF-401-NA) was from R & D; anti-IκB-α (C-21; sc-371), anti-Ub (P4D1; sc-8017), and anti-ASC (sc-514414) was from Santa Cruz; anti-HA (16B12; MMS-101P) was from Covance; anti-K63 ubiquitin (HWA4C4; BML-PW0600) was from Enzo life science; anti-K48 ubiquitin (Apu2; 05-1307) was from Millipore; anti-flag (F3165) and anti-β-actin (AC-15; A 1978) were from Sigma; anti-mouse IRDyeTM 680 (926-68070) and anti-rabbit IRDyeTM 800 (926-32211) were from LI-COR Biosciences; anti-mouse-HRP and anti-rabbit-HRP were from Promega.

    Techniques: Immunofluorescence, Staining, Immunostaining, Infection, Expressing, Construct, Mutagenesis

    Pellino2 mediates LPS-induced ubiquitination and activation of NLRP3. a-c Immunoblot analysis of NLRP3 and ubiquitin in cell lysates (Input) and immunoprecipitated (IP) NLRP3 samples ( a ) or NLRP3 and K63-linked ubiquitin (K63-ubq) in K63-TUBE-FLAG elution and cell lysates ( b ) or NLRP3 and K48-ubq in K48-TUBE-FLAG elution and cell lysates ( c ) from WT and Peli2 −/− BMDMs treated with a 100 ng/ml LPS for the indicated times. d ELISA of IL-1β in medium from WT and Peli2 −/− BMDMs treated with 100 ng/ml LPS for 2 h followed by sequential treatment with 1 μM MCC950 for 1 h and 2.5 mM ATP for 30 min. UT, untreated. e, f Immunoblot analysis of ubiquitin and NLRP3 in lysates (Input) and immunoprecipitated (IP) NLRP3 samples ( e ) or NLRP3 and K63-ubq in K63-TUBE-FLAG elution and cell lysates ( f ) from WT and Peli2 −/− BMDMs pre-treated with 1 μM MCC950 for 1 h followed by treatment with 100 ng/ml LPS for the indicated times. g Peli2 −/− BMDMs were infected with MSCV as control (MSCV-Ctrl) or with MSCV containing an expression construct encoding myc-tagged murine Pellino2 (MSCV-Peli2). Immunoblot analysis of NLRP3 and myc in lysates (Input) and immunoprecipitated (IP) myc samples from MSCV-infected cells treated with 100 ng/ml LPS for the indicated times. β-Actin was used as loading controls. * p

    Journal: Nature Communications

    Article Title: The E3 ubiquitin ligase Pellino2 mediates priming of the NLRP3 inflammasome

    doi: 10.1038/s41467-018-03669-z

    Figure Lengend Snippet: Pellino2 mediates LPS-induced ubiquitination and activation of NLRP3. a-c Immunoblot analysis of NLRP3 and ubiquitin in cell lysates (Input) and immunoprecipitated (IP) NLRP3 samples ( a ) or NLRP3 and K63-linked ubiquitin (K63-ubq) in K63-TUBE-FLAG elution and cell lysates ( b ) or NLRP3 and K48-ubq in K48-TUBE-FLAG elution and cell lysates ( c ) from WT and Peli2 −/− BMDMs treated with a 100 ng/ml LPS for the indicated times. d ELISA of IL-1β in medium from WT and Peli2 −/− BMDMs treated with 100 ng/ml LPS for 2 h followed by sequential treatment with 1 μM MCC950 for 1 h and 2.5 mM ATP for 30 min. UT, untreated. e, f Immunoblot analysis of ubiquitin and NLRP3 in lysates (Input) and immunoprecipitated (IP) NLRP3 samples ( e ) or NLRP3 and K63-ubq in K63-TUBE-FLAG elution and cell lysates ( f ) from WT and Peli2 −/− BMDMs pre-treated with 1 μM MCC950 for 1 h followed by treatment with 100 ng/ml LPS for the indicated times. g Peli2 −/− BMDMs were infected with MSCV as control (MSCV-Ctrl) or with MSCV containing an expression construct encoding myc-tagged murine Pellino2 (MSCV-Peli2). Immunoblot analysis of NLRP3 and myc in lysates (Input) and immunoprecipitated (IP) myc samples from MSCV-infected cells treated with 100 ng/ml LPS for the indicated times. β-Actin was used as loading controls. * p

    Article Snippet: Anti-p-ERK (9101), anti-ERK (9102), anti-p-IκBα (9246), anti-pIKK (14938), anti-p65 (3033) anti-p-P38 (9211), anti-P38 (9212), anti-p-Jnk (9251), anti-Jnk (9252), anti-myc (2276), anti-IRAK1 (4504), anti-IRAK4 (4363), and anti-human caspase 1 p20 (4199) were from CellSignaling; anti-mouse caspase1 p20 (AG-20B-0042-C100) and anti-mouse NLRP3 (AG-20B-0014-C100) was from adipogen; anti-mouse IL1β (AF-401-NA) was from R & D; anti-IκB-α (C-21; sc-371), anti-Ub (P4D1; sc-8017), and anti-ASC (sc-514414) was from Santa Cruz; anti-HA (16B12; MMS-101P) was from Covance; anti-K63 ubiquitin (HWA4C4; BML-PW0600) was from Enzo life science; anti-K48 ubiquitin (Apu2; 05-1307) was from Millipore; anti-flag (F3165) and anti-β-actin (AC-15; A 1978) were from Sigma; anti-mouse IRDyeTM 680 (926-68070) and anti-rabbit IRDyeTM 800 (926-32211) were from LI-COR Biosciences; anti-mouse-HRP and anti-rabbit-HRP were from Promega.

    Techniques: Activation Assay, Immunoprecipitation, Enzyme-linked Immunosorbent Assay, Infection, Expressing, Construct

    Pellino2 mediates activation of the NLRP3 pathway. ELISA of a IL-1β and b IL-18, and c LDH assay of medium from WT and Peli2 −/− BMDMs treated with 100 ng/ml LPS for 3 h with or without further stimulation with 2.5 mM ATP for 1 h. UT, untreated. d Immunoblot analysis of IL-1β and Caspase-1 in medium (Sup) and lysates from WT and Peli2 −/− BMDMs stimulated with 100 ng/ml LPS for 3 h with or without further stimulation with 2.5 mM ATP for 1 h or transfection of Poly (dA:dT) (1 μg/ml) for 6 h. ELISA of e IL-1β and f IL-18 and g LDH assay of medium from WT and Peli2 −/− BMDMs treated with 100 ng/ml LPS for 3 h with or without further stimulation with 5 mM Nigericin for 1 h. h Immunoblot analysis of IL-1β and Caspase-1 in medium (Sup) and lysates from WT and Peli2 −/− BMDMs stimulated with 100 ng/ml LPS for 3 h with or without further stimulation with 5 mM Nigericin for 1 h or transfection of Poly (dA:dT) (1 μg/ml) for 6 h. i ELISA of IL-1β of medium from WT and Peli2 −/− BMDMs treated with 100 ng/ml LPS for 3 h with or without further stimulation with the indicated concentrations of Alum for 6 h. j ELISA of IL-1β in medium from peritoneal-resident macrophages isolated from WT and Peli2 −/− mice. Cells were treated with 100 ng/ml of LPS for 3 h with or without further stimulation with 2.5 mM ATP for 1 h. k-m Human THP1 cells were transfected with human Pellino2-specific siRNA or control siRNA. k Quantitative RT-PCR analysis of PELI2 expression in transfected cells. l ELISA of IL-1β in medium from transfected THP1 cells stimulated with 100 ng/ml LPS for 6 h with or without further treatment with 2.5 mM ATP or 5 mM Nigericin for 1 h. m Immunoblot analysis of IL-1β and Caspase-1 in medium (Sup) and lysates from transfected THP1 cells stimulated with 100 ng/ml LPS for 6 h with or without further stimulation with 2.5 mM ATP or 5 mM Nigericin for 1 h. β-Actin was used as loading controls. * p

    Journal: Nature Communications

    Article Title: The E3 ubiquitin ligase Pellino2 mediates priming of the NLRP3 inflammasome

    doi: 10.1038/s41467-018-03669-z

    Figure Lengend Snippet: Pellino2 mediates activation of the NLRP3 pathway. ELISA of a IL-1β and b IL-18, and c LDH assay of medium from WT and Peli2 −/− BMDMs treated with 100 ng/ml LPS for 3 h with or without further stimulation with 2.5 mM ATP for 1 h. UT, untreated. d Immunoblot analysis of IL-1β and Caspase-1 in medium (Sup) and lysates from WT and Peli2 −/− BMDMs stimulated with 100 ng/ml LPS for 3 h with or without further stimulation with 2.5 mM ATP for 1 h or transfection of Poly (dA:dT) (1 μg/ml) for 6 h. ELISA of e IL-1β and f IL-18 and g LDH assay of medium from WT and Peli2 −/− BMDMs treated with 100 ng/ml LPS for 3 h with or without further stimulation with 5 mM Nigericin for 1 h. h Immunoblot analysis of IL-1β and Caspase-1 in medium (Sup) and lysates from WT and Peli2 −/− BMDMs stimulated with 100 ng/ml LPS for 3 h with or without further stimulation with 5 mM Nigericin for 1 h or transfection of Poly (dA:dT) (1 μg/ml) for 6 h. i ELISA of IL-1β of medium from WT and Peli2 −/− BMDMs treated with 100 ng/ml LPS for 3 h with or without further stimulation with the indicated concentrations of Alum for 6 h. j ELISA of IL-1β in medium from peritoneal-resident macrophages isolated from WT and Peli2 −/− mice. Cells were treated with 100 ng/ml of LPS for 3 h with or without further stimulation with 2.5 mM ATP for 1 h. k-m Human THP1 cells were transfected with human Pellino2-specific siRNA or control siRNA. k Quantitative RT-PCR analysis of PELI2 expression in transfected cells. l ELISA of IL-1β in medium from transfected THP1 cells stimulated with 100 ng/ml LPS for 6 h with or without further treatment with 2.5 mM ATP or 5 mM Nigericin for 1 h. m Immunoblot analysis of IL-1β and Caspase-1 in medium (Sup) and lysates from transfected THP1 cells stimulated with 100 ng/ml LPS for 6 h with or without further stimulation with 2.5 mM ATP or 5 mM Nigericin for 1 h. β-Actin was used as loading controls. * p

    Article Snippet: Anti-p-ERK (9101), anti-ERK (9102), anti-p-IκBα (9246), anti-pIKK (14938), anti-p65 (3033) anti-p-P38 (9211), anti-P38 (9212), anti-p-Jnk (9251), anti-Jnk (9252), anti-myc (2276), anti-IRAK1 (4504), anti-IRAK4 (4363), and anti-human caspase 1 p20 (4199) were from CellSignaling; anti-mouse caspase1 p20 (AG-20B-0042-C100) and anti-mouse NLRP3 (AG-20B-0014-C100) was from adipogen; anti-mouse IL1β (AF-401-NA) was from R & D; anti-IκB-α (C-21; sc-371), anti-Ub (P4D1; sc-8017), and anti-ASC (sc-514414) was from Santa Cruz; anti-HA (16B12; MMS-101P) was from Covance; anti-K63 ubiquitin (HWA4C4; BML-PW0600) was from Enzo life science; anti-K48 ubiquitin (Apu2; 05-1307) was from Millipore; anti-flag (F3165) and anti-β-actin (AC-15; A 1978) were from Sigma; anti-mouse IRDyeTM 680 (926-68070) and anti-rabbit IRDyeTM 800 (926-32211) were from LI-COR Biosciences; anti-mouse-HRP and anti-rabbit-HRP were from Promega.

    Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Lactate Dehydrogenase Assay, Transfection, Isolation, Mouse Assay, Quantitative RT-PCR, Expressing

    Pellino2 mediates activation of the NLRP3 pathway in response to bacterial infection. a-f WT and Peli2 −/− BMDMs were infected with a, b C. rodentium , c, d E.coli , or e, f P. aeruginosa (PA01 strain) at a multiplicity of infection (MOI) of 100. a, c, e ELISA of IL-1β, IL-18, and CXCL1 in medium from BMDMs infected for 6 h. b, d Immunoblot analysis of Caspase-11 in lysates from cells infected for 0–6 h. f Immunoblot analysis of IL-1β and Caspase-1 in lysates from cells infected with PAO1 for 3 h. β-Actin was used as loading controls. g ELISA of IL-1β, IL-18, and IL-6 in peritoneal lavage from WT and Peli2 −/− mice previously infected for 10 h by intraperitoneal injection of PAO1 (1.5 × 10 7 CFU). * p

    Journal: Nature Communications

    Article Title: The E3 ubiquitin ligase Pellino2 mediates priming of the NLRP3 inflammasome

    doi: 10.1038/s41467-018-03669-z

    Figure Lengend Snippet: Pellino2 mediates activation of the NLRP3 pathway in response to bacterial infection. a-f WT and Peli2 −/− BMDMs were infected with a, b C. rodentium , c, d E.coli , or e, f P. aeruginosa (PA01 strain) at a multiplicity of infection (MOI) of 100. a, c, e ELISA of IL-1β, IL-18, and CXCL1 in medium from BMDMs infected for 6 h. b, d Immunoblot analysis of Caspase-11 in lysates from cells infected for 0–6 h. f Immunoblot analysis of IL-1β and Caspase-1 in lysates from cells infected with PAO1 for 3 h. β-Actin was used as loading controls. g ELISA of IL-1β, IL-18, and IL-6 in peritoneal lavage from WT and Peli2 −/− mice previously infected for 10 h by intraperitoneal injection of PAO1 (1.5 × 10 7 CFU). * p

    Article Snippet: Anti-p-ERK (9101), anti-ERK (9102), anti-p-IκBα (9246), anti-pIKK (14938), anti-p65 (3033) anti-p-P38 (9211), anti-P38 (9212), anti-p-Jnk (9251), anti-Jnk (9252), anti-myc (2276), anti-IRAK1 (4504), anti-IRAK4 (4363), and anti-human caspase 1 p20 (4199) were from CellSignaling; anti-mouse caspase1 p20 (AG-20B-0042-C100) and anti-mouse NLRP3 (AG-20B-0014-C100) was from adipogen; anti-mouse IL1β (AF-401-NA) was from R & D; anti-IκB-α (C-21; sc-371), anti-Ub (P4D1; sc-8017), and anti-ASC (sc-514414) was from Santa Cruz; anti-HA (16B12; MMS-101P) was from Covance; anti-K63 ubiquitin (HWA4C4; BML-PW0600) was from Enzo life science; anti-K48 ubiquitin (Apu2; 05-1307) was from Millipore; anti-flag (F3165) and anti-β-actin (AC-15; A 1978) were from Sigma; anti-mouse IRDyeTM 680 (926-68070) and anti-rabbit IRDyeTM 800 (926-32211) were from LI-COR Biosciences; anti-mouse-HRP and anti-rabbit-HRP were from Promega.

    Techniques: Activation Assay, Infection, Enzyme-linked Immunosorbent Assay, Mouse Assay, Injection

    Seesaw effect between CAV1 and HER2 proteins. a Association between CAV1 and HER2 in gastric (NCIN87, KATOIII), bladder (HT1197, UMUC14, UMUC3), breast (MDAMB231, MCF-7, BT474, SKBR3), pancreatic (BxPc3, MiaPaCa, Suit2) and prostate (LnCap, Pc3) cancer cells. The level of CAV1 ( y -axis) and HER2 ( x -axis) are expressed as the ratio of protein/β-actin control, as determined by western blot analysis (upper panel). The non-parametric one-tailed Spearman test was used to determine the correlation coefficients. R HER2/CAV1 , ratio of HER2-to-CAV1 protein levels as determined by western blot analysis from total extracts (lower panel). b Confocal images of immunofluorescence staining of HER2 in s.c. NCIN87 gastric tumors from athymic nude mice, showing that HER2 does not exhibit a predominant membrane staining. Scale bars: 100 μm and 50 μm (inset). c Confocal images of immunofluorescence staining of HER2 and CAV1 in s.c. NCIN87 gastric tumors from athymic nude mice. HER2 presence at the cell membrane in cells where CAV1 is absent (cell clusters 1 and 3) and HER2 almost absent at the cell membrane in cells where CAV1 is present (cell clusters 2 and 4). Scale bars: 50 μm. d Confocal images and quantification (mean ± S.E.M, n = 3) of immunofluorescence staining of HER2 and CAV1 in human HER2-positive gastric tumors containing high (Patient 1) and low (Patient 2) expression of CAV1. HER2 predominant presence at the cell membrane in CAV1-negative tumors (Patient 2). Scale bars: 50 μm. e Western blot analysis of the distribution of HER2 and CAV1 in caveolae fractions isolated from NCIN87 cells. f Western blot of HER2 and CAV1 in the total lysates of NCIN87 cells after blocking protein synthesis with 80 μg mL −1 CHX for 0, 12, 24, 30, 48, 60, 72, and 96 h. CHX cyclohexamide. g HER2 and CAV1 have similar half-lives. Half-lives calculated after western blot analysis of Fig. 1f. Density of western blot bands was quantified by scanning densitometry with ImageJ software. Half-lives were calculated as the time required for protein decrease to 50% of its initial level (mean ± S.E.M, n = 4)

    Journal: Nature Communications

    Article Title: Caveolin-1 mediates cellular distribution of HER2 and affects trastuzumab binding and therapeutic efficacy

    doi: 10.1038/s41467-018-07608-w

    Figure Lengend Snippet: Seesaw effect between CAV1 and HER2 proteins. a Association between CAV1 and HER2 in gastric (NCIN87, KATOIII), bladder (HT1197, UMUC14, UMUC3), breast (MDAMB231, MCF-7, BT474, SKBR3), pancreatic (BxPc3, MiaPaCa, Suit2) and prostate (LnCap, Pc3) cancer cells. The level of CAV1 ( y -axis) and HER2 ( x -axis) are expressed as the ratio of protein/β-actin control, as determined by western blot analysis (upper panel). The non-parametric one-tailed Spearman test was used to determine the correlation coefficients. R HER2/CAV1 , ratio of HER2-to-CAV1 protein levels as determined by western blot analysis from total extracts (lower panel). b Confocal images of immunofluorescence staining of HER2 in s.c. NCIN87 gastric tumors from athymic nude mice, showing that HER2 does not exhibit a predominant membrane staining. Scale bars: 100 μm and 50 μm (inset). c Confocal images of immunofluorescence staining of HER2 and CAV1 in s.c. NCIN87 gastric tumors from athymic nude mice. HER2 presence at the cell membrane in cells where CAV1 is absent (cell clusters 1 and 3) and HER2 almost absent at the cell membrane in cells where CAV1 is present (cell clusters 2 and 4). Scale bars: 50 μm. d Confocal images and quantification (mean ± S.E.M, n = 3) of immunofluorescence staining of HER2 and CAV1 in human HER2-positive gastric tumors containing high (Patient 1) and low (Patient 2) expression of CAV1. HER2 predominant presence at the cell membrane in CAV1-negative tumors (Patient 2). Scale bars: 50 μm. e Western blot analysis of the distribution of HER2 and CAV1 in caveolae fractions isolated from NCIN87 cells. f Western blot of HER2 and CAV1 in the total lysates of NCIN87 cells after blocking protein synthesis with 80 μg mL −1 CHX for 0, 12, 24, 30, 48, 60, 72, and 96 h. CHX cyclohexamide. g HER2 and CAV1 have similar half-lives. Half-lives calculated after western blot analysis of Fig. 1f. Density of western blot bands was quantified by scanning densitometry with ImageJ software. Half-lives were calculated as the time required for protein decrease to 50% of its initial level (mean ± S.E.M, n = 4)

    Article Snippet: Following electrophoresis and transfer to polyvinylidene difluoride (PVDF) membranes (Bio-Rad), the blots were incubated in 5% (m/v) BSA in Tris-buffered saline buffer-Tween (TBS-T, Cell Signaling Technology) and probed with mouse anti β-actin 1:20,000 (Sigma, A1978), rabbit anti-HER2 1:800 (Abcam, ab131490), rabbit anti-H ER2 phospho Y1139 1:500 (Abcam, ab53290), and rabbit anti-CAV1 1:500 (Abcam, ab2910) antibodies.

    Techniques: Western Blot, One-tailed Test, Immunofluorescence, Staining, Mouse Assay, Expressing, Isolation, Blocking Assay, Software

    REV-ERB agonist SR9011 inhibit autophagy a–b, SR90011 treatment reduces the number of autophagosomes both in MCF7 and T47D, n= biological independent samples MCF7 20µM 24h n=9 (mock), n=4 (SR9011) ** P =0.0056, T47D 48h 20µM n=5 (mock), n=4 (SR9011) ** P =0.0079; c–d, SR9011 induces accumulation of p62 as shown by immunofluorescence both in MCF7 and T47D n= biological independent samples 48h MCF7 p62 n=3 (mock), n=4 (SR9011) * P =0.0286; 48h T47D n=5 (mock, SR9011) ** P =0.004; e, Accumulation of p62 is confirmed by immunoblot (48h, 20µM A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved Caspase 3 and TUNEL assay (n= biological independent samples n=4 mock 48h, n=5 SR9011 p62, n=3 48h SR9011; n=6 (mock, SR9011 72h p62), n=10 (mock 72h), n=8 (SR9011 72h) A375 20µM, Cl. Casp. 3 48h * P =0.0286; Cl. Casp. 3 72h **** P

    Journal: Nature

    Article Title: Pharmacological activation of REV-ERBs is lethal in cancer and oncogene induced senescence

    doi: 10.1038/nature25170

    Figure Lengend Snippet: REV-ERB agonist SR9011 inhibit autophagy a–b, SR90011 treatment reduces the number of autophagosomes both in MCF7 and T47D, n= biological independent samples MCF7 20µM 24h n=9 (mock), n=4 (SR9011) ** P =0.0056, T47D 48h 20µM n=5 (mock), n=4 (SR9011) ** P =0.0079; c–d, SR9011 induces accumulation of p62 as shown by immunofluorescence both in MCF7 and T47D n= biological independent samples 48h MCF7 p62 n=3 (mock), n=4 (SR9011) * P =0.0286; 48h T47D n=5 (mock, SR9011) ** P =0.004; e, Accumulation of p62 is confirmed by immunoblot (48h, 20µM A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved Caspase 3 and TUNEL assay (n= biological independent samples n=4 mock 48h, n=5 SR9011 p62, n=3 48h SR9011; n=6 (mock, SR9011 72h p62), n=10 (mock 72h), n=8 (SR9011 72h) A375 20µM, Cl. Casp. 3 48h * P =0.0286; Cl. Casp. 3 72h **** P

    Article Snippet: Apoptosis was evaluated by immunostaining of cleaved caspase 3 (Cell signaling #9664 1:200) and by TUNEL assay using In Situ Cell Death Detection Kit, Fluoresceine or TMR red (Roche).

    Techniques: Immunofluorescence, Inhibition, TUNEL Assay

    SR9009 and SR9011 treatment evokes an apoptotic response and induces inhibition of autophagy in OIS cells a, Proliferation assay shows that REV-ERBs agonists impair viability of OIS cells (6 days, 20µM). b–c, Immunofluorescence assay for cleaved Caspase 3 and TUNEL assay shows apoptosis induction specifically in OIS (n=biological independent samples, n=7 mock, n=9 SR9009, n=14 SR9011, 72h, 20µM; one-way ANOVA, Cl. Casp 3 **** P

    Journal: Nature

    Article Title: Pharmacological activation of REV-ERBs is lethal in cancer and oncogene induced senescence

    doi: 10.1038/nature25170

    Figure Lengend Snippet: SR9009 and SR9011 treatment evokes an apoptotic response and induces inhibition of autophagy in OIS cells a, Proliferation assay shows that REV-ERBs agonists impair viability of OIS cells (6 days, 20µM). b–c, Immunofluorescence assay for cleaved Caspase 3 and TUNEL assay shows apoptosis induction specifically in OIS (n=biological independent samples, n=7 mock, n=9 SR9009, n=14 SR9011, 72h, 20µM; one-way ANOVA, Cl. Casp 3 **** P

    Article Snippet: Apoptosis was evaluated by immunostaining of cleaved caspase 3 (Cell signaling #9664 1:200) and by TUNEL assay using In Situ Cell Death Detection Kit, Fluoresceine or TMR red (Roche).

    Techniques: Inhibition, Proliferation Assay, Immunofluorescence, TUNEL Assay

    REV-ERBs agonist SR9009 inhibits autophagy a–b , SR9009 treatment reduces the number of autophagosomes, as shown by immunofluorescence of LC3B, (n=biological independent samples MCF7 (n=6 mock), (n=5 SR9009) and T47D (n=5 mock) (n=4 SR9009) Mann–Whitney test one-tailed MCF7 20µM 24h * P =0.0152, T47D 20µM 48h ** P =0.0079; c–d SR9009 induces accumulation of p62 as shown by immunofluorescence; n= biological independent samples MCF7 (n=3 mock), (n=8 SR9009) and T47D (n=5 mock), (n=4 SR9009) Mann–Whitney test one-tailed 48h MCF7 p62 ** P =0.0061; 48h T47D ** P =0.0079; e, Inhibition of autophagy is confirmed by the immunoblot for p62 (20µM 48h, A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved Caspase 3 and TUNEL assay; n=biological independent samples, Mann–Whitney test one-tailed, A375 20µM Cl. Casp. 3 48h (n=3) * P =0.0179; Cl. Casp. 3 72h (n=7) **** P

    Journal: Nature

    Article Title: Pharmacological activation of REV-ERBs is lethal in cancer and oncogene induced senescence

    doi: 10.1038/nature25170

    Figure Lengend Snippet: REV-ERBs agonist SR9009 inhibits autophagy a–b , SR9009 treatment reduces the number of autophagosomes, as shown by immunofluorescence of LC3B, (n=biological independent samples MCF7 (n=6 mock), (n=5 SR9009) and T47D (n=5 mock) (n=4 SR9009) Mann–Whitney test one-tailed MCF7 20µM 24h * P =0.0152, T47D 20µM 48h ** P =0.0079; c–d SR9009 induces accumulation of p62 as shown by immunofluorescence; n= biological independent samples MCF7 (n=3 mock), (n=8 SR9009) and T47D (n=5 mock), (n=4 SR9009) Mann–Whitney test one-tailed 48h MCF7 p62 ** P =0.0061; 48h T47D ** P =0.0079; e, Inhibition of autophagy is confirmed by the immunoblot for p62 (20µM 48h, A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved Caspase 3 and TUNEL assay; n=biological independent samples, Mann–Whitney test one-tailed, A375 20µM Cl. Casp. 3 48h (n=3) * P =0.0179; Cl. Casp. 3 72h (n=7) **** P

    Article Snippet: Apoptosis was evaluated by immunostaining of cleaved caspase 3 (Cell signaling #9664 1:200) and by TUNEL assay using In Situ Cell Death Detection Kit, Fluoresceine or TMR red (Roche).

    Techniques: Immunofluorescence, MANN-WHITNEY, One-tailed Test, Inhibition, TUNEL Assay

    Neurotoxicity of CORT in primary mouse hippocampal neurons. a Dose dependence analyzed by one-way ANOVA ( n = 3). b Immunostaining for cleaved caspase-3 (C.Casp-3) after treatment with CORT for 24 h or staurosporine (STS) for 6 h. Arrows indicates apoptotic, condensed nucleus. Scale bar, 10 μm. c Western blotting analysis of C.Casp-3 after treatment with CORT for 24 h or STS for 6 h. d Western blotting analysis of LC3-II after CORT treatment for 24 h. Bafilomycin A1 (Baf.A1; 20 nM) was added 2 h before sampling. e Quantification of p62 and LC3-II analyzed by two-way ANOVA ( n = 8). Data are mean ± SEM. * p

    Journal: Molecular Brain

    Article Title: Chronic restraint stress induces hippocampal memory deficits by impairing insulin signaling

    doi: 10.1186/s13041-018-0381-8

    Figure Lengend Snippet: Neurotoxicity of CORT in primary mouse hippocampal neurons. a Dose dependence analyzed by one-way ANOVA ( n = 3). b Immunostaining for cleaved caspase-3 (C.Casp-3) after treatment with CORT for 24 h or staurosporine (STS) for 6 h. Arrows indicates apoptotic, condensed nucleus. Scale bar, 10 μm. c Western blotting analysis of C.Casp-3 after treatment with CORT for 24 h or STS for 6 h. d Western blotting analysis of LC3-II after CORT treatment for 24 h. Bafilomycin A1 (Baf.A1; 20 nM) was added 2 h before sampling. e Quantification of p62 and LC3-II analyzed by two-way ANOVA ( n = 8). Data are mean ± SEM. * p

    Article Snippet: Antibodies against IR subunit β (3025), p-Akt-S473 (9271), Akt (9272), p-mTOR-S2448 (2971), mTOR (2972), and cleaved caspase-3 (C.Casp-3; 9664) were purchased form Cell Signaling Technology.

    Techniques: Immunostaining, Western Blot, Sampling

    IL-31R signaling did not influence the differentiation of CD4 + T cell sub-populations. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with anti-CD3/anti-CD28 under Th1-, Th2- or Th17- polarizing conditions. Cells were assayed for the transcription factors T-bet, GATA-3, ROR-γt and cytokines IFN-γ, IL-4, IL-17 by flow cytometry. No difference on the differentiation of Th1(A), Th2 (B) and Th17 (C) was found between WT ( n =4) and IL-31RA KO mice ( n =4). All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated three times. P, polarization; NP, non-polarization.

    Journal: Biology Open

    Article Title: IL-31 plays dual roles in lung inflammation in an OVA-induced murine asthma model

    doi: 10.1242/bio.036244

    Figure Lengend Snippet: IL-31R signaling did not influence the differentiation of CD4 + T cell sub-populations. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with anti-CD3/anti-CD28 under Th1-, Th2- or Th17- polarizing conditions. Cells were assayed for the transcription factors T-bet, GATA-3, ROR-γt and cytokines IFN-γ, IL-4, IL-17 by flow cytometry. No difference on the differentiation of Th1(A), Th2 (B) and Th17 (C) was found between WT ( n =4) and IL-31RA KO mice ( n =4). All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated three times. P, polarization; NP, non-polarization.

    Article Snippet: T cells at 2 million cells/ml were washed and resuspended in 2 μM CFSE (Invitrogen) staining solution for 10 min, and CFSE fluorescence was measured by flow cytometry at 96 h after culture with 1 μl/ml anti-CD3 (CAT#85-16-0032-81)/anti-CD28 (CAT#85-16-0281-81) (both eBioscience).

    Techniques: Purification, Mouse Assay, Flow Cytometry, Cytometry, Two Tailed Test

    IL-31R signaling suppressed the proliferation of CD4 + T cells. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with anti-CD3/anti-CD28. The activation ( n =4) (A) and proliferation ( n =4) (B) of CD4 + T cells were assayed by flow cytometry. All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated twice. Ctrl, control; Act, activation.

    Journal: Biology Open

    Article Title: IL-31 plays dual roles in lung inflammation in an OVA-induced murine asthma model

    doi: 10.1242/bio.036244

    Figure Lengend Snippet: IL-31R signaling suppressed the proliferation of CD4 + T cells. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with anti-CD3/anti-CD28. The activation ( n =4) (A) and proliferation ( n =4) (B) of CD4 + T cells were assayed by flow cytometry. All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated twice. Ctrl, control; Act, activation.

    Article Snippet: T cells at 2 million cells/ml were washed and resuspended in 2 μM CFSE (Invitrogen) staining solution for 10 min, and CFSE fluorescence was measured by flow cytometry at 96 h after culture with 1 μl/ml anti-CD3 (CAT#85-16-0032-81)/anti-CD28 (CAT#85-16-0281-81) (both eBioscience).

    Techniques: Purification, Mouse Assay, Activation Assay, Flow Cytometry, Cytometry, Two Tailed Test, Activated Clotting Time Assay

    The effects of daily different dwelling times on cellular protein expression and cell survival. Expression of E-cadherin in mesothelial cells (MCs) was assessed on day 3, and cell counts were determined on days 3 and 6. A) The expression of E-cadherin, exhibited significant down-regulation with increasing dwell time (N = 4). B) The cell count decreased with a longer daily dwell time, where the maximal decrease was evident in the 24 hour group. On day 3, the 24 hour group demonstrated a significantly lower cell count compared to the phosphate-buffered saline (PBS) group. On day 6, the 16 and 24 hour groups demonstrated a significantly lower cell count compared to the PBS group. Additionally, the 24 hour group also demonstrated a significantly lower cell count compared to the transforming growth factor-β1 (TGF-β1) group (N = 4) (* P

    Journal: BMC Nephrology

    Article Title: Shorter daily dwelling time in peritoneal dialysis attenuates the epithelial-to-mesenchymal transition of mesothelial cells

    doi: 10.1186/1471-2369-15-35

    Figure Lengend Snippet: The effects of daily different dwelling times on cellular protein expression and cell survival. Expression of E-cadherin in mesothelial cells (MCs) was assessed on day 3, and cell counts were determined on days 3 and 6. A) The expression of E-cadherin, exhibited significant down-regulation with increasing dwell time (N = 4). B) The cell count decreased with a longer daily dwell time, where the maximal decrease was evident in the 24 hour group. On day 3, the 24 hour group demonstrated a significantly lower cell count compared to the phosphate-buffered saline (PBS) group. On day 6, the 16 and 24 hour groups demonstrated a significantly lower cell count compared to the PBS group. Additionally, the 24 hour group also demonstrated a significantly lower cell count compared to the transforming growth factor-β1 (TGF-β1) group (N = 4) (* P

    Article Snippet: Western blotting for E-cadherin To validate the changes in EMT of MCs, the expression of the epithelial marker, E-cadherin (anti-E-cadherin; BD Bioscience, Bedford, MA), were identified [ ].

    Techniques: Expressing, Cell Counting