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mab860 55d  (R&D Systems)
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( A ) Immunofluorescent assay for colocalization of AXL with B19V virions. CD36 + EPCs cells were infected with B19V at an MOI of ~5000 vgc per cell for 1.5 hours at 4°C. The cells were then washed with ice-cold PBS, then cytospun onto slides, fixed, permeabilized, and stained with an anti-B19V capsid antibody (clone <t>MAB860-55D).</t> Confocal images were taken under a confocal microscope. Nuclei were stained with DAPI (blue). Scale bar, 10 μm. ( B to D ) Kinetics of the in vitro interaction between AXL and VP1u using Ni-NTA biosensors. The binding kinetics depicts the associations and dissociations of 4 μM rAXL EC with GST-VP1u at the indicated concentrations (B). Binding kinetics of 4 μM AXL His with 4 and 8 μM GST was used as a control (C). The binding parameters of AXL and GST-VP1u are shown (D). Equilibrium dissociation constant K D value represents the ratio of dissociation [ K d (1/s)] and association [ K a (1/Ms)] computed from the real-time binding curves of rAXL EC to GST-VP1u. The values are shown with means ± SDs based on at least three repeated experiments.
Mab860 55d, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab860+55d/Human%2FMouse+BID+Antibody/pmc09833669-199-16-31
Average 93 stars, based on 1 article reviews
mab860 55d - by Bioz Stars, 2026-08
93/100 stars
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( A ) Immunofluorescent assay for colocalization of AXL with B19V virions. CD36 + EPCs cells were infected with B19V at an MOI of ~5000 vgc per cell for 1.5 hours at 4°C. The cells were then washed with ice-cold PBS, then cytospun onto slides, fixed, permeabilized, and stained with an anti-B19V capsid antibody (clone MAB860-55D). Confocal images were taken under a confocal microscope. Nuclei were stained with DAPI (blue). Scale bar, 10 μm. ( B to D ) Kinetics of the in vitro interaction between AXL and VP1u using Ni-NTA biosensors. The binding kinetics depicts the associations and dissociations of 4 μM rAXL EC with GST-VP1u at the indicated concentrations (B). Binding kinetics of 4 μM AXL His with 4 and 8 μM GST was used as a control (C). The binding parameters of AXL and GST-VP1u are shown (D). Equilibrium dissociation constant K D value represents the ratio of dissociation [ K d (1/s)] and association [ K a (1/Ms)] computed from the real-time binding curves of rAXL EC to GST-VP1u. The values are shown with means ± SDs based on at least three repeated experiments.

Journal: Science Advances

Article Title: Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors

doi: 10.1126/sciadv.ade0869

Figure Lengend Snippet: ( A ) Immunofluorescent assay for colocalization of AXL with B19V virions. CD36 + EPCs cells were infected with B19V at an MOI of ~5000 vgc per cell for 1.5 hours at 4°C. The cells were then washed with ice-cold PBS, then cytospun onto slides, fixed, permeabilized, and stained with an anti-B19V capsid antibody (clone MAB860-55D). Confocal images were taken under a confocal microscope. Nuclei were stained with DAPI (blue). Scale bar, 10 μm. ( B to D ) Kinetics of the in vitro interaction between AXL and VP1u using Ni-NTA biosensors. The binding kinetics depicts the associations and dissociations of 4 μM rAXL EC with GST-VP1u at the indicated concentrations (B). Binding kinetics of 4 μM AXL His with 4 and 8 μM GST was used as a control (C). The binding parameters of AXL and GST-VP1u are shown (D). Equilibrium dissociation constant K D value represents the ratio of dissociation [ K d (1/s)] and association [ K a (1/Ms)] computed from the real-time binding curves of rAXL EC to GST-VP1u. The values are shown with means ± SDs based on at least three repeated experiments.

Article Snippet: The fixed cells were then incubated with an anti-B19V capsid antibody [#MAB8292/clone 521-5D ( ) or #MAb860-55D ( , )] to detect intact B19V virions or costained with anti-AXL antibody (#AF154, R&D Systems).

Techniques: Infection, Staining, Microscopy, In Vitro, Binding Assay, Control