mab247 Search Results


mouse monoclonal anti human il 15 ab  (R&D Systems)
96
R&D Systems mouse monoclonal anti human il 15 ab
Mouse Monoclonal Anti Human Il 15 Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab247/Human+IL-15+Antibody/pm11673509-57-17-22
Average 96 stars, based on 1 article reviews
mouse monoclonal anti human il 15 ab - by Bioz Stars, 2026-09
96/100 stars
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sandwich elisa test  (R&D Systems)
93
R&D Systems sandwich elisa test
Fig. 2 Results of second-step screening process of IL-15-CAT fusions when expressed in E. coli DH5a. The vertical <t>axis</t> <t>(solubility</t> test) corresponds to the <t>ELISA</t> absorbance of cell extracts incubated in plates coated with Mab247, revealed with an anti-CAT polyclonal antibody. The horizontal axis (functionality test) corresponds to the ELISA absorbance of cell extracts incubated in plates coated with IL-15Ra and revealed with Mab247. Filled squares represent clones selected at cam ,400 mM; filled circle, clones selected at cam 800 mM; filled triangle, clones selected at cam 1200 mM; open square, clones selected at cam 1500 mM.
Sandwich Elisa Test, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab247/Human+IL-15+Antibody/pm21109548-59-7-17
Average 93 stars, based on 1 article reviews
sandwich elisa test - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results


Fig. 2 Results of second-step screening process of IL-15-CAT fusions when expressed in E. coli DH5a. The vertical axis (solubility test) corresponds to the ELISA absorbance of cell extracts incubated in plates coated with Mab247, revealed with an anti-CAT polyclonal antibody. The horizontal axis (functionality test) corresponds to the ELISA absorbance of cell extracts incubated in plates coated with IL-15Ra and revealed with Mab247. Filled squares represent clones selected at cam ,400 mM; filled circle, clones selected at cam 800 mM; filled triangle, clones selected at cam 1200 mM; open square, clones selected at cam 1500 mM.

Journal: Protein engineering, design & selection : PEDS

Article Title: Evolution of interleukin-15 for higher E. coli expression and solubility.

doi: 10.1093/protein/gzq107

Figure Lengend Snippet: Fig. 2 Results of second-step screening process of IL-15-CAT fusions when expressed in E. coli DH5a. The vertical axis (solubility test) corresponds to the ELISA absorbance of cell extracts incubated in plates coated with Mab247, revealed with an anti-CAT polyclonal antibody. The horizontal axis (functionality test) corresponds to the ELISA absorbance of cell extracts incubated in plates coated with IL-15Ra and revealed with Mab247. Filled squares represent clones selected at cam ,400 mM; filled circle, clones selected at cam 800 mM; filled triangle, clones selected at cam 1200 mM; open square, clones selected at cam 1500 mM.

Article Snippet: To screen mutants for their solubility, a sandwich ELISA test was used with Mab247 (anti-human IL-15 IgG, R&D system), dilution 1 mg/ml, as the capture antibody and a two-step detection system with a polyclonal rabbit anti-CAT IgG (Sigma) and a goat anti-rabbit IgG conjugated with peroxidase (Sigma).

Techniques: Solubility, Enzyme-linked Immunosorbent Assay, Incubation, Clone Assay