Journal: Reproduction (Cambridge, England)

Article Title: Platelet-derived growth factor modulates the primordial to primary follicle transition.

doi: 10.1530/rep.1.00978

Figure Lengend Snippet: Figure 1 Effect of PDGF-treatment on primordial to primary follicle transition in cultured ovaries. Ovaries from 4-day-old rats were placed into culture for 14 days. Cultured ovaries were treated with PDGF, anti- PDGF neutralizing antibody, KITL or were left untreated as controls. After culture all ovaries were fixed, stained and subjected to morphological analysis. The follicles per ovary cross-section were categorized as being either primordial or developing (which includes all follicles having undergone the primordial to primary transition). Data are presented as the mean percentageGS.E.M. with data pooled from four separate experiments (nZ6–12 ovaries per treatment group). One-way ANOVA showed a significant (P!0.0001) effect of treatment. Asterisks indicate that the percentage of developing follicles for a treatment is significantly (P!0.05) different than that of the control by post-hoc Dunnet’s test.

Article Snippet: Treatments during organ culture included recombinant rat PDGF-AB heterodimer (R&D Systems, Inc.; Minneapolis, MN, USA) at 50 ng/ml, rat KITL (Amgen; Thousand Oaks, CA, USA) at 50 ng/ml, anti-PDGF neutralizing antibody (R&D Systems) at 20 mg/ml, www.reproduction-online.org human NRG1-b1 extracellular domain (R&D Systems) at 50 ng/ml, human NRG1-b1 EGF domain (R&D Systems) at 50 ng/ml, or rat VEGF (R&D Systems) at 50 ng/ml.

Techniques: Cell Culture, Staining, Control

Journal: Reproduction (Cambridge, England)

Article Title: Platelet-derived growth factor modulates the primordial to primary follicle transition.

doi: 10.1530/rep.1.00978

Figure Lengend Snippet: Figure 3 ImmunohistochemicallocalizationofPDGFproteininculturedovaries.ThepresenceofPDGFproteinisindicatedbyadarkbrownstaininovary sections. A) Four-day-old ovaries cultured for 14 days showing PDGF staining at high intensity in oocytes. B) Control cultured ovary section stained using non-immune IgG as a primary antibody. C) Freshly isolated 4-day-old ovaries showing PDGF staining in the oocytes of primordial follicles. D) Control freshly isolated ovary section stained using non-immune IgG as a primary antibody. Images are representative of two different experiments using ovaries from different rats. Microscope magnificationZ400!. Total microscope magnification at the time of image capture was 400!.

Article Snippet: Treatments during organ culture included recombinant rat PDGF-AB heterodimer (R&D Systems, Inc.; Minneapolis, MN, USA) at 50 ng/ml, rat KITL (Amgen; Thousand Oaks, CA, USA) at 50 ng/ml, anti-PDGF neutralizing antibody (R&D Systems) at 20 mg/ml, www.reproduction-online.org human NRG1-b1 extracellular domain (R&D Systems) at 50 ng/ml, human NRG1-b1 EGF domain (R&D Systems) at 50 ng/ml, or rat VEGF (R&D Systems) at 50 ng/ml.

Techniques: Cell Culture, Staining, Control, Isolation, Microscopy

Journal: Reproduction (Cambridge, England)

Article Title: Platelet-derived growth factor modulates the primordial to primary follicle transition.

doi: 10.1530/rep.1.00978

Figure Lengend Snippet: Figure 5 Kit ligand (KITL) mRNA expression in cultured ovaries in response to PDGF-treatment. Ovaries from 4-day-old rats were placed into culture and treated with PDGF or were not treated for 2 days. Real- time PCR was performed on RNA isolated from cultured whole ovaries to determine levels of KITL mRNA expression. Data are expressed as KITL mRNA/S2 mRNA normalized to untreated control values. Data are from three separate experiments with the meanGS.E.M. presented. Asterisk indicates a significant difference (P!0.05) from control as determined by a one-sample t-test.

Article Snippet: Treatments during organ culture included recombinant rat PDGF-AB heterodimer (R&D Systems, Inc.; Minneapolis, MN, USA) at 50 ng/ml, rat KITL (Amgen; Thousand Oaks, CA, USA) at 50 ng/ml, anti-PDGF neutralizing antibody (R&D Systems) at 20 mg/ml, www.reproduction-online.org human NRG1-b1 extracellular domain (R&D Systems) at 50 ng/ml, human NRG1-b1 EGF domain (R&D Systems) at 50 ng/ml, or rat VEGF (R&D Systems) at 50 ng/ml.

Techniques: Expressing, Cell Culture, Real-time Polymerase Chain Reaction, Isolation, Control

Journal: Reproduction (Cambridge, England)

Article Title: Platelet-derived growth factor modulates the primordial to primary follicle transition.

doi: 10.1530/rep.1.00978

Figure Lengend Snippet: Figure 6 A model of the regulation of the primordial to primary follicle transition by paracrine growth factors, including the actions of platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF), leukemia inhibitory factor (LIF), bone morphogenetic protein-4 (BMP4), keratinocyte growth factor (KGF), Anti-Mu¨llerian Hormone/Mu¨llerian inhibitory substance (AMH/MIS), and insulin.

Article Snippet: Treatments during organ culture included recombinant rat PDGF-AB heterodimer (R&D Systems, Inc.; Minneapolis, MN, USA) at 50 ng/ml, rat KITL (Amgen; Thousand Oaks, CA, USA) at 50 ng/ml, anti-PDGF neutralizing antibody (R&D Systems) at 20 mg/ml, www.reproduction-online.org human NRG1-b1 extracellular domain (R&D Systems) at 50 ng/ml, human NRG1-b1 EGF domain (R&D Systems) at 50 ng/ml, or rat VEGF (R&D Systems) at 50 ng/ml.

Techniques: Derivative Assay