mab Search Results


resource source identifier antibodies mouse monoclonal igg1 anti rac1 cytoskeleton  (Cytoskeleton Inc)
94
Cytoskeleton Inc resource source identifier antibodies mouse monoclonal igg1 anti rac1 cytoskeleton
Figure 1. <t>Rac1</t> signaling is increased in MMTV-PyMT-driven metastatic breast cancer (A) Schematic of Rac1-FRET biosensor in FRET conformation upon GTP loading and Rac1 activation in cells crossed to the MMTV-PyMT-driven breast cancer model. (B) Representative images and quantification of upregulated Rac1 activity in primary MMTV-PyMT-driven mammary tumors compared to WT mammary glands (n = 7 mice per condition, 991 cells in total). (C) Spatiotemporal monitoring of live Rac1 activity in the context of the native tumor microenvironment showing the local vasculature (Qdot655) and second harmonic generation (SHG) imaging visualizing the local ECM monitored by optical window imaging in primary tumors (i). Longitudinal imaging can be achieved using these windows and further improved by image stabilization, allowing for the assessment of Rac1 activity in relation to tumor microenvironment (ii). (D) Tracking of Rac1 activity in relation to the proximity of cells to local tumor vasculature, with example excerpts showing cells proximal and distal to the local vasculature (n = 5 mice, 166 cells). (E) Upregulation of Rac1 activity at the invasive border of primary tumors as quantified through an optical window (n = 5 mice, 180 cells). Columns show averages, and error bars represent SEM; scale bars, 50 mm. Unpaired Welch’s t test, ****p < 0.0001 and *p < 0.05 (B and E); one-way ANOVA, *p < 0.05 (D).
Resource Source Identifier Antibodies Mouse Monoclonal Igg1 Anti Rac1 Cytoskeleton, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab/Anti-Rac1+mouse+Mab/pm34525350-202-2-10
Average 94 stars, based on 1 article reviews
resource source identifier antibodies mouse monoclonal igg1 anti rac1 cytoskeleton - by Bioz Stars, 2026-09
94/100 stars
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antibody anti fyb monoclonal lorne cat  (Lorne Laboratories)
86
Lorne Laboratories antibody anti fyb monoclonal lorne cat
Figure 1. <t>Rac1</t> signaling is increased in MMTV-PyMT-driven metastatic breast cancer (A) Schematic of Rac1-FRET biosensor in FRET conformation upon GTP loading and Rac1 activation in cells crossed to the MMTV-PyMT-driven breast cancer model. (B) Representative images and quantification of upregulated Rac1 activity in primary MMTV-PyMT-driven mammary tumors compared to WT mammary glands (n = 7 mice per condition, 991 cells in total). (C) Spatiotemporal monitoring of live Rac1 activity in the context of the native tumor microenvironment showing the local vasculature (Qdot655) and second harmonic generation (SHG) imaging visualizing the local ECM monitored by optical window imaging in primary tumors (i). Longitudinal imaging can be achieved using these windows and further improved by image stabilization, allowing for the assessment of Rac1 activity in relation to tumor microenvironment (ii). (D) Tracking of Rac1 activity in relation to the proximity of cells to local tumor vasculature, with example excerpts showing cells proximal and distal to the local vasculature (n = 5 mice, 166 cells). (E) Upregulation of Rac1 activity at the invasive border of primary tumors as quantified through an optical window (n = 5 mice, 180 cells). Columns show averages, and error bars represent SEM; scale bars, 50 mm. Unpaired Welch’s t test, ****p < 0.0001 and *p < 0.05 (B and E); one-way ANOVA, *p < 0.05 (D).
Antibody Anti Fyb Monoclonal Lorne Cat, supplied by Lorne Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab/10__7554_slash_elife__45829-295-171-174
Average 86 stars, based on 1 article reviews
antibody anti fyb monoclonal lorne cat - by Bioz Stars, 2026-09
86/100 stars
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antibody duoclone monoclonal lorne cat  (Lorne Laboratories)
86
Lorne Laboratories antibody duoclone monoclonal lorne cat
Figure 1. <t>Rac1</t> signaling is increased in MMTV-PyMT-driven metastatic breast cancer (A) Schematic of Rac1-FRET biosensor in FRET conformation upon GTP loading and Rac1 activation in cells crossed to the MMTV-PyMT-driven breast cancer model. (B) Representative images and quantification of upregulated Rac1 activity in primary MMTV-PyMT-driven mammary tumors compared to WT mammary glands (n = 7 mice per condition, 991 cells in total). (C) Spatiotemporal monitoring of live Rac1 activity in the context of the native tumor microenvironment showing the local vasculature (Qdot655) and second harmonic generation (SHG) imaging visualizing the local ECM monitored by optical window imaging in primary tumors (i). Longitudinal imaging can be achieved using these windows and further improved by image stabilization, allowing for the assessment of Rac1 activity in relation to tumor microenvironment (ii). (D) Tracking of Rac1 activity in relation to the proximity of cells to local tumor vasculature, with example excerpts showing cells proximal and distal to the local vasculature (n = 5 mice, 166 cells). (E) Upregulation of Rac1 activity at the invasive border of primary tumors as quantified through an optical window (n = 5 mice, 180 cells). Columns show averages, and error bars represent SEM; scale bars, 50 mm. Unpaired Welch’s t test, ****p < 0.0001 and *p < 0.05 (B and E); one-way ANOVA, *p < 0.05 (D).
Antibody Duoclone Monoclonal Lorne Cat, supplied by Lorne Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab/10__7554_slash_elife__45829-295-156-159
Average 86 stars, based on 1 article reviews
antibody duoclone monoclonal lorne cat - by Bioz Stars, 2026-09
86/100 stars
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anti mouse monoclonal antibodies  (Boster Bio)
91
Boster Bio anti mouse monoclonal antibodies
Figure 1. <t>Rac1</t> signaling is increased in MMTV-PyMT-driven metastatic breast cancer (A) Schematic of Rac1-FRET biosensor in FRET conformation upon GTP loading and Rac1 activation in cells crossed to the MMTV-PyMT-driven breast cancer model. (B) Representative images and quantification of upregulated Rac1 activity in primary MMTV-PyMT-driven mammary tumors compared to WT mammary glands (n = 7 mice per condition, 991 cells in total). (C) Spatiotemporal monitoring of live Rac1 activity in the context of the native tumor microenvironment showing the local vasculature (Qdot655) and second harmonic generation (SHG) imaging visualizing the local ECM monitored by optical window imaging in primary tumors (i). Longitudinal imaging can be achieved using these windows and further improved by image stabilization, allowing for the assessment of Rac1 activity in relation to tumor microenvironment (ii). (D) Tracking of Rac1 activity in relation to the proximity of cells to local tumor vasculature, with example excerpts showing cells proximal and distal to the local vasculature (n = 5 mice, 166 cells). (E) Upregulation of Rac1 activity at the invasive border of primary tumors as quantified through an optical window (n = 5 mice, 180 cells). Columns show averages, and error bars represent SEM; scale bars, 50 mm. Unpaired Welch’s t test, ****p < 0.0001 and *p < 0.05 (B and E); one-way ANOVA, *p < 0.05 (D).
Anti Mouse Monoclonal Antibodies, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab/Anti-ATM+Monoclonal+Antibody/pmc04305631-75-4-16
Average 91 stars, based on 1 article reviews
anti mouse monoclonal antibodies - by Bioz Stars, 2026-09
91/100 stars
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mouse anti sars cov 2 n monoclonal antibody  (R&D Systems)
95
R&D Systems mouse anti sars cov 2 n monoclonal antibody
Figure 1. <t>Rac1</t> signaling is increased in MMTV-PyMT-driven metastatic breast cancer (A) Schematic of Rac1-FRET biosensor in FRET conformation upon GTP loading and Rac1 activation in cells crossed to the MMTV-PyMT-driven breast cancer model. (B) Representative images and quantification of upregulated Rac1 activity in primary MMTV-PyMT-driven mammary tumors compared to WT mammary glands (n = 7 mice per condition, 991 cells in total). (C) Spatiotemporal monitoring of live Rac1 activity in the context of the native tumor microenvironment showing the local vasculature (Qdot655) and second harmonic generation (SHG) imaging visualizing the local ECM monitored by optical window imaging in primary tumors (i). Longitudinal imaging can be achieved using these windows and further improved by image stabilization, allowing for the assessment of Rac1 activity in relation to tumor microenvironment (ii). (D) Tracking of Rac1 activity in relation to the proximity of cells to local tumor vasculature, with example excerpts showing cells proximal and distal to the local vasculature (n = 5 mice, 166 cells). (E) Upregulation of Rac1 activity at the invasive border of primary tumors as quantified through an optical window (n = 5 mice, 180 cells). Columns show averages, and error bars represent SEM; scale bars, 50 mm. Unpaired Welch’s t test, ****p < 0.0001 and *p < 0.05 (B and E); one-way ANOVA, *p < 0.05 (D).
Mouse Anti Sars Cov 2 N Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab/SARS-CoV-2+Nucleocapsid+MAb+(Clone+1035111)/pmc08942852-317-24-29
Average 95 stars, based on 1 article reviews
mouse anti sars cov 2 n monoclonal antibody - by Bioz Stars, 2026-09
95/100 stars
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pge2 elisa kit  (Cayman Chemical)
97
Cayman Chemical pge2 elisa kit
Figure 1. <t>Rac1</t> signaling is increased in MMTV-PyMT-driven metastatic breast cancer (A) Schematic of Rac1-FRET biosensor in FRET conformation upon GTP loading and Rac1 activation in cells crossed to the MMTV-PyMT-driven breast cancer model. (B) Representative images and quantification of upregulated Rac1 activity in primary MMTV-PyMT-driven mammary tumors compared to WT mammary glands (n = 7 mice per condition, 991 cells in total). (C) Spatiotemporal monitoring of live Rac1 activity in the context of the native tumor microenvironment showing the local vasculature (Qdot655) and second harmonic generation (SHG) imaging visualizing the local ECM monitored by optical window imaging in primary tumors (i). Longitudinal imaging can be achieved using these windows and further improved by image stabilization, allowing for the assessment of Rac1 activity in relation to tumor microenvironment (ii). (D) Tracking of Rac1 activity in relation to the proximity of cells to local tumor vasculature, with example excerpts showing cells proximal and distal to the local vasculature (n = 5 mice, 166 cells). (E) Upregulation of Rac1 activity at the invasive border of primary tumors as quantified through an optical window (n = 5 mice, 180 cells). Columns show averages, and error bars represent SEM; scale bars, 50 mm. Unpaired Welch’s t test, ****p < 0.0001 and *p < 0.05 (B and E); one-way ANOVA, *p < 0.05 (D).
Pge2 Elisa Kit, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab/Prostaglandin+E2+ELISA+Kit+-+Monoclonal/pm31900335-147-6-10
Average 97 stars, based on 1 article reviews
pge2 elisa kit - by Bioz Stars, 2026-09
97/100 stars
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cleaved caspase 9  (Boster Bio)
94
Boster Bio cleaved caspase 9
UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of <t>caspase-9,</t> and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars
Cleaved Caspase 9, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab/Anti-Caspase+9+CASP9+Monoclonal+Antibody/pmc12866510-84-38-37
Average 94 stars, based on 1 article reviews
cleaved caspase 9 - by Bioz Stars, 2026-09
94/100 stars
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pv 6002  (OriGene)
93
OriGene pv 6002
UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of <t>caspase-9,</t> and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars
Pv 6002, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab/C2+Mouse+Monoclonal+Antibody/10__1016_slash_j__esmoop__2025__105561-42-42-43
Average 93 stars, based on 1 article reviews
pv 6002 - by Bioz Stars, 2026-09
93/100 stars
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mouse anti e1  (OriGene)
90
OriGene mouse anti e1
UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of <t>caspase-9,</t> and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars
Mouse Anti E1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab/E7+Mouse+Monoclonal+Antibody/pm25486085-93-6-8
Average 90 stars, based on 1 article reviews
mouse anti e1 - by Bioz Stars, 2026-09
90/100 stars
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mouse anti human rgs5  (OriGene)
90
OriGene mouse anti human rgs5
UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of <t>caspase-9,</t> and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars
Mouse Anti Human Rgs5, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab/RGS5+Mouse+Monoclonal+Antibody/pmc07295949-99-28-43
Average 90 stars, based on 1 article reviews
mouse anti human rgs5 - by Bioz Stars, 2026-09
90/100 stars
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ta347049  (OriGene)
93
OriGene ta347049
UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of <t>caspase-9,</t> and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars
Ta347049, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab/BRG1+(SMARCA4)+Mouse+Monoclonal+Antibody/us12473334-497-35-42
Average 93 stars, based on 1 article reviews
ta347049 - by Bioz Stars, 2026-09
93/100 stars
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1a4 antibody  (OriGene)
92
OriGene 1a4 antibody
UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of <t>caspase-9,</t> and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars
1a4 Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab/Human+IgA2+(Fc+specific)+Mouse+Monoclonal+Antibody/pmc08862096-51-2-4
Average 92 stars, based on 1 article reviews
1a4 antibody - by Bioz Stars, 2026-09
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Image Search Results


Figure 1. Rac1 signaling is increased in MMTV-PyMT-driven metastatic breast cancer (A) Schematic of Rac1-FRET biosensor in FRET conformation upon GTP loading and Rac1 activation in cells crossed to the MMTV-PyMT-driven breast cancer model. (B) Representative images and quantification of upregulated Rac1 activity in primary MMTV-PyMT-driven mammary tumors compared to WT mammary glands (n = 7 mice per condition, 991 cells in total). (C) Spatiotemporal monitoring of live Rac1 activity in the context of the native tumor microenvironment showing the local vasculature (Qdot655) and second harmonic generation (SHG) imaging visualizing the local ECM monitored by optical window imaging in primary tumors (i). Longitudinal imaging can be achieved using these windows and further improved by image stabilization, allowing for the assessment of Rac1 activity in relation to tumor microenvironment (ii). (D) Tracking of Rac1 activity in relation to the proximity of cells to local tumor vasculature, with example excerpts showing cells proximal and distal to the local vasculature (n = 5 mice, 166 cells). (E) Upregulation of Rac1 activity at the invasive border of primary tumors as quantified through an optical window (n = 5 mice, 180 cells). Columns show averages, and error bars represent SEM; scale bars, 50 mm. Unpaired Welch’s t test, ****p < 0.0001 and *p < 0.05 (B and E); one-way ANOVA, *p < 0.05 (D).

Journal: Cell reports

Article Title: Optimizing metastatic-cascade-dependent Rac1 targeting in breast cancer: Guidance using optical window intravital FRET imaging.

doi: 10.1016/j.celrep.2021.109689

Figure Lengend Snippet: Figure 1. Rac1 signaling is increased in MMTV-PyMT-driven metastatic breast cancer (A) Schematic of Rac1-FRET biosensor in FRET conformation upon GTP loading and Rac1 activation in cells crossed to the MMTV-PyMT-driven breast cancer model. (B) Representative images and quantification of upregulated Rac1 activity in primary MMTV-PyMT-driven mammary tumors compared to WT mammary glands (n = 7 mice per condition, 991 cells in total). (C) Spatiotemporal monitoring of live Rac1 activity in the context of the native tumor microenvironment showing the local vasculature (Qdot655) and second harmonic generation (SHG) imaging visualizing the local ECM monitored by optical window imaging in primary tumors (i). Longitudinal imaging can be achieved using these windows and further improved by image stabilization, allowing for the assessment of Rac1 activity in relation to tumor microenvironment (ii). (D) Tracking of Rac1 activity in relation to the proximity of cells to local tumor vasculature, with example excerpts showing cells proximal and distal to the local vasculature (n = 5 mice, 166 cells). (E) Upregulation of Rac1 activity at the invasive border of primary tumors as quantified through an optical window (n = 5 mice, 180 cells). Columns show averages, and error bars represent SEM; scale bars, 50 mm. Unpaired Welch’s t test, ****p < 0.0001 and *p < 0.05 (B and E); one-way ANOVA, *p < 0.05 (D).

Article Snippet: Reagent or Resource Source Identifier Antibodies Mouse monoclonal IgG1 anti-Rac1 Cytoskeleton Cat# ARC03, RRID:AB_10709099 Rabbit poly-clonal IgG anti-GFP Abcam Cat# ab290, RRID:AB_303395 Rabbit monoclonal IgG1 anti-GAPDH Cell Signaling Cat# 2118, RRID:AB_561053 Sheep-anti-Mouse IgG HRP-linked GE healthcare Cat# NA931, RRID:AB_772210 Donkey-anti-Rabbit IgG HRP-linked GE healthcare Cat# NA934, RRID:AB_772206 Rabbit monoclonal IgG1 anti-Ki67 ThermoFisher Scientific Cat# RM-9106-S1, RRID:AB_149792 Rabbit polyclonal anti-cleaved Caspase-3 Cell Signaling Cat# 9661, RRID:AB_2341188 Rabbit monoclonal anti-Cyclin B1 (EPR17060) Abcam Cat# ab181593, RRID:AB_2820245 Rabbit polyclonal anti- Cyclin A2 (C-19) Santa Cruz Biotechnology Cat# sc-596 Rabbit polyclonal anti-PAK1 Cell Signaling Cat# 2602, RRID:AB_330222 Rabbit polyclonal anti-PAK1 (Ser199/204)/PAK2 (Ser192/197) Cell Signaling Cat# 2605, RRID:AB_2160222 Mouse Monoclonal anti-beta-actin (clone AC-15) Merck (SigmaAldrich) Cat# A5441, RRID:AB_476744 Bacterial and virus strains E. coli DH5a ThermoFisher Scientific Cat# 18265017 Chemicals, peptides and recombinant proteins Qtracker 655 Vascular Label ThermoFisher Scientific Cat# Q21021MP AnnexinV-Cy5 BioVision Cat# 1013 Propidium iodide Merck, Sigma-Aldrich Cat# P4170 SalI Promega Cat# R6051 NdeI New England Biolabs Cat# R0111S Rac1 Pull-down Activation Assay Biochem Kit CytoSkeleton Inc. Cat# BK035 Experimental models: Cell lines PyMT 20065 (from mouse 20065, female) SEARCHBreast https://searchbreast.org/ MMTV-PyMT;Rac1-FRET (from mouse 8625, female) This study N/A MMTV-PyMT;Rac1-FRET;P-Rex1 ± (from mouse 138509, female) This study N/A Experimental models: Organisms/strains Rac1-FRET mice (ubiquitously expressed, ‘‘ON’’) Prof. Heidi CE Welch, Babraham Institute, UK, Johnsson et al. 2014 N/A MMTV-Her2 mice The Jackson Laboratory Stock# 002376 MMTV-PyMT mice The Jackson Laboratory Stock# 002374 K14-CreER mice The Jackson Laboratory Stock# 005107 LSL-K-Ras G12D mice The Jackson Laboratory Stock# 008179 Rac1-flox mice Prof L. Machesky, Prof O. Sansom, CRUK Beatson Institute, University of Glasgow N/A P-Rex1 KO mice Prof. Heidi CE Welch, Babraham Institute, UK, Welch et al. 2005 N/A FVB/NJ (Ausb) mice The Jackson Laboratory Stock# 001800 NOD.Cg-Prkdc < scid > IL2rg < tm1Wjl > /SzJ (Ausb) mice The Jackson Laboratory Stock# 005557 BALB/c-Fox1nu (Ausb) mice Charles River Strain# 194 (Continued on next page) Cell Reports 36, 109689, September 14, 2021 e1

Techniques: Activation Assay, Activity Assay, Imaging

UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of caspase-9, and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars

Journal: Cancer Cell International

Article Title: Silencing Uracil-DNA glycosylase inhibits colorectal cancer progression

doi: 10.1186/s12935-025-04089-y

Figure Lengend Snippet: UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of caspase-9, and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars

Article Snippet: The membranes were incubated overnight at 4 °C with the following primary antibodies: UNG, P70 S6K, p-P70 S6K(S424) (1:1000, Bioworld), β-actin (1:1000, Abcam), cleaved caspase-3, AMPKα1/AMPKα2, p-AMPKα1(Thr183)/AMPKα2(Thr172) (1:1000, Beyotime), BAX, BCL2, AKT1/2/3, mTOR, and p-mTOR (Ser2448) (1:1000; BOSTER), cleaved caspase-9, p-AKT(Ser473), ERK1/2, and p-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) (1:1000; ZEN BIO), and GAPDH (1:1000; Proteintech).

Techniques: Protein-Protein interactions, Western Blot, Knockdown