m7000 system Search Results


m-7000  (Hitachi Ltd)
99
Hitachi Ltd m-7000
M 7000, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m-7000/product/Hitachi Ltd
Average 99 stars, based on 1 article reviews
m-7000 - by Bioz Stars, 2026-05
99/100 stars
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quantikine il7 elisa kit  (R&D Systems)
93
R&D Systems quantikine il7 elisa kit
Figure 1. Soluble factors mediate FRC activation and mitochondrial imbalance. A, Experimental scheme used to investigate tumor-draining factors in vivo. B, Draining lymph nodes were harvested from female C57BL/6 mice that had received 10 daily subcutaneous doses of PBS, RPMI medium, or B16.F10 TCM into the shoulder. Flow cytometric analysis allowed reporting of the total number of lymph node cells (left), flow cytometry gating scheme, FRCs (right), and FRC Pdpn histogram (furthest right). n ¼ 4 to 9 animals per group. C, qRT-PCR analysis of Pdpn (left), Thy1 (middle), and <t>Il7</t> (right) in FRCs cultured in vitro and treated for 4 days with CCM, B16.F10 TCM, or 4T1 TCM. n ¼ 3 to 10 independent experiments. D, IL7 protein quantification in supernatants of FRCs cultured in vitro and treated with CCM or B16.F10 TCM, assessed by <t>ELISA.</t> n ¼ 4 independent experiments. Flow cytometric quantification of Pdpn protein (E) and Thy1 protein (F) expression for FRCs cultured in vitro and treated with CCM or 4T1 TCM for 4 days. n ¼ 3 independent experiments. G, Flow cytometric analysis of TMRM incorporation by FRCs treated for 4 days with CCM or B16 TCM. n ¼ 4 independent experiments performed in triplicate. H, OCR of FRCs treated with CCM or B16.F10 TCM at baseline and in response to oligomycin, FCCP, and rotenone plus antimycin A. One representative of three experiments with five replicates. I, Baseline OCR from CCM or B16.F10 TCM-treated FRCs. n ¼ 3 experiments with 5 replicates. J, Representative confocal imaging of live cells treated with CCM or B16.F10 TCM. Green, Mitochondria (Mitotracker green); blue, nuclei (Hoechst). Scale bar: 7 mm. Data are mean with SEM. Significance (, P < 0.05; , P < 0.01; , P < 0.001; and , P < 0.0001) was determined by unpaired two-tailed t test or one-way ANOVA with Tukey post hoc (B). s.c., subcutaneous; B16, B16.F10; max, maximum; NS, not significant.
Quantikine Il7 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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mouse il 7 quantikine elisa kit m700  (R&D Systems)
93
R&D Systems mouse il 7 quantikine elisa kit m700
Figure 1. Soluble factors mediate FRC activation and mitochondrial imbalance. A, Experimental scheme used to investigate tumor-draining factors in vivo. B, Draining lymph nodes were harvested from female C57BL/6 mice that had received 10 daily subcutaneous doses of PBS, RPMI medium, or B16.F10 TCM into the shoulder. Flow cytometric analysis allowed reporting of the total number of lymph node cells (left), flow cytometry gating scheme, FRCs (right), and FRC Pdpn histogram (furthest right). n ¼ 4 to 9 animals per group. C, qRT-PCR analysis of Pdpn (left), Thy1 (middle), and <t>Il7</t> (right) in FRCs cultured in vitro and treated for 4 days with CCM, B16.F10 TCM, or 4T1 TCM. n ¼ 3 to 10 independent experiments. D, IL7 protein quantification in supernatants of FRCs cultured in vitro and treated with CCM or B16.F10 TCM, assessed by <t>ELISA.</t> n ¼ 4 independent experiments. Flow cytometric quantification of Pdpn protein (E) and Thy1 protein (F) expression for FRCs cultured in vitro and treated with CCM or 4T1 TCM for 4 days. n ¼ 3 independent experiments. G, Flow cytometric analysis of TMRM incorporation by FRCs treated for 4 days with CCM or B16 TCM. n ¼ 4 independent experiments performed in triplicate. H, OCR of FRCs treated with CCM or B16.F10 TCM at baseline and in response to oligomycin, FCCP, and rotenone plus antimycin A. One representative of three experiments with five replicates. I, Baseline OCR from CCM or B16.F10 TCM-treated FRCs. n ¼ 3 experiments with 5 replicates. J, Representative confocal imaging of live cells treated with CCM or B16.F10 TCM. Green, Mitochondria (Mitotracker green); blue, nuclei (Hoechst). Scale bar: 7 mm. Data are mean with SEM. Significance (, P < 0.05; , P < 0.01; , P < 0.001; and , P < 0.0001) was determined by unpaired two-tailed t test or one-way ANOVA with Tukey post hoc (B). s.c., subcutaneous; B16, B16.F10; max, maximum; NS, not significant.
Mouse Il 7 Quantikine Elisa Kit M700, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse il 7 quantikine elisa kit m700/product/R&D Systems
Average 93 stars, based on 1 article reviews
mouse il 7 quantikine elisa kit m700 - by Bioz Stars, 2026-05
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m7000  (McScience Inc)
90
McScience Inc m7000
Figure 1. Soluble factors mediate FRC activation and mitochondrial imbalance. A, Experimental scheme used to investigate tumor-draining factors in vivo. B, Draining lymph nodes were harvested from female C57BL/6 mice that had received 10 daily subcutaneous doses of PBS, RPMI medium, or B16.F10 TCM into the shoulder. Flow cytometric analysis allowed reporting of the total number of lymph node cells (left), flow cytometry gating scheme, FRCs (right), and FRC Pdpn histogram (furthest right). n ¼ 4 to 9 animals per group. C, qRT-PCR analysis of Pdpn (left), Thy1 (middle), and <t>Il7</t> (right) in FRCs cultured in vitro and treated for 4 days with CCM, B16.F10 TCM, or 4T1 TCM. n ¼ 3 to 10 independent experiments. D, IL7 protein quantification in supernatants of FRCs cultured in vitro and treated with CCM or B16.F10 TCM, assessed by <t>ELISA.</t> n ¼ 4 independent experiments. Flow cytometric quantification of Pdpn protein (E) and Thy1 protein (F) expression for FRCs cultured in vitro and treated with CCM or 4T1 TCM for 4 days. n ¼ 3 independent experiments. G, Flow cytometric analysis of TMRM incorporation by FRCs treated for 4 days with CCM or B16 TCM. n ¼ 4 independent experiments performed in triplicate. H, OCR of FRCs treated with CCM or B16.F10 TCM at baseline and in response to oligomycin, FCCP, and rotenone plus antimycin A. One representative of three experiments with five replicates. I, Baseline OCR from CCM or B16.F10 TCM-treated FRCs. n ¼ 3 experiments with 5 replicates. J, Representative confocal imaging of live cells treated with CCM or B16.F10 TCM. Green, Mitochondria (Mitotracker green); blue, nuclei (Hoechst). Scale bar: 7 mm. Data are mean with SEM. Significance (, P < 0.05; , P < 0.01; , P < 0.001; and , P < 0.0001) was determined by unpaired two-tailed t test or one-way ANOVA with Tukey post hoc (B). s.c., subcutaneous; B16, B16.F10; max, maximum; NS, not significant.
M7000, supplied by McScience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m7000/product/McScience Inc
Average 90 stars, based on 1 article reviews
m7000 - by Bioz Stars, 2026-05
90/100 stars
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evos m7000  (Gibthai Co Ltd)
90
Gibthai Co Ltd evos m7000
Figure 1. Soluble factors mediate FRC activation and mitochondrial imbalance. A, Experimental scheme used to investigate tumor-draining factors in vivo. B, Draining lymph nodes were harvested from female C57BL/6 mice that had received 10 daily subcutaneous doses of PBS, RPMI medium, or B16.F10 TCM into the shoulder. Flow cytometric analysis allowed reporting of the total number of lymph node cells (left), flow cytometry gating scheme, FRCs (right), and FRC Pdpn histogram (furthest right). n ¼ 4 to 9 animals per group. C, qRT-PCR analysis of Pdpn (left), Thy1 (middle), and <t>Il7</t> (right) in FRCs cultured in vitro and treated for 4 days with CCM, B16.F10 TCM, or 4T1 TCM. n ¼ 3 to 10 independent experiments. D, IL7 protein quantification in supernatants of FRCs cultured in vitro and treated with CCM or B16.F10 TCM, assessed by <t>ELISA.</t> n ¼ 4 independent experiments. Flow cytometric quantification of Pdpn protein (E) and Thy1 protein (F) expression for FRCs cultured in vitro and treated with CCM or 4T1 TCM for 4 days. n ¼ 3 independent experiments. G, Flow cytometric analysis of TMRM incorporation by FRCs treated for 4 days with CCM or B16 TCM. n ¼ 4 independent experiments performed in triplicate. H, OCR of FRCs treated with CCM or B16.F10 TCM at baseline and in response to oligomycin, FCCP, and rotenone plus antimycin A. One representative of three experiments with five replicates. I, Baseline OCR from CCM or B16.F10 TCM-treated FRCs. n ¼ 3 experiments with 5 replicates. J, Representative confocal imaging of live cells treated with CCM or B16.F10 TCM. Green, Mitochondria (Mitotracker green); blue, nuclei (Hoechst). Scale bar: 7 mm. Data are mean with SEM. Significance (, P < 0.05; , P < 0.01; , P < 0.001; and , P < 0.0001) was determined by unpaired two-tailed t test or one-way ANOVA with Tukey post hoc (B). s.c., subcutaneous; B16, B16.F10; max, maximum; NS, not significant.
Evos M7000, supplied by Gibthai Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/evos m7000/product/Gibthai Co Ltd
Average 90 stars, based on 1 article reviews
evos m7000 - by Bioz Stars, 2026-05
90/100 stars
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flexible hose fitok ss-mm8-ft8-m7000  (FITOK GmbH)
90
FITOK GmbH flexible hose fitok ss-mm8-ft8-m7000
Figure 1. Soluble factors mediate FRC activation and mitochondrial imbalance. A, Experimental scheme used to investigate tumor-draining factors in vivo. B, Draining lymph nodes were harvested from female C57BL/6 mice that had received 10 daily subcutaneous doses of PBS, RPMI medium, or B16.F10 TCM into the shoulder. Flow cytometric analysis allowed reporting of the total number of lymph node cells (left), flow cytometry gating scheme, FRCs (right), and FRC Pdpn histogram (furthest right). n ¼ 4 to 9 animals per group. C, qRT-PCR analysis of Pdpn (left), Thy1 (middle), and <t>Il7</t> (right) in FRCs cultured in vitro and treated for 4 days with CCM, B16.F10 TCM, or 4T1 TCM. n ¼ 3 to 10 independent experiments. D, IL7 protein quantification in supernatants of FRCs cultured in vitro and treated with CCM or B16.F10 TCM, assessed by <t>ELISA.</t> n ¼ 4 independent experiments. Flow cytometric quantification of Pdpn protein (E) and Thy1 protein (F) expression for FRCs cultured in vitro and treated with CCM or 4T1 TCM for 4 days. n ¼ 3 independent experiments. G, Flow cytometric analysis of TMRM incorporation by FRCs treated for 4 days with CCM or B16 TCM. n ¼ 4 independent experiments performed in triplicate. H, OCR of FRCs treated with CCM or B16.F10 TCM at baseline and in response to oligomycin, FCCP, and rotenone plus antimycin A. One representative of three experiments with five replicates. I, Baseline OCR from CCM or B16.F10 TCM-treated FRCs. n ¼ 3 experiments with 5 replicates. J, Representative confocal imaging of live cells treated with CCM or B16.F10 TCM. Green, Mitochondria (Mitotracker green); blue, nuclei (Hoechst). Scale bar: 7 mm. Data are mean with SEM. Significance (, P < 0.05; , P < 0.01; , P < 0.001; and , P < 0.0001) was determined by unpaired two-tailed t test or one-way ANOVA with Tukey post hoc (B). s.c., subcutaneous; B16, B16.F10; max, maximum; NS, not significant.
Flexible Hose Fitok Ss Mm8 Ft8 M7000, supplied by FITOK GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flexible hose fitok ss-mm8-ft8-m7000/product/FITOK GmbH
Average 90 stars, based on 1 article reviews
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evos m7000 system  (KU Leuven)
90
KU Leuven evos m7000 system
Figure 1. Soluble factors mediate FRC activation and mitochondrial imbalance. A, Experimental scheme used to investigate tumor-draining factors in vivo. B, Draining lymph nodes were harvested from female C57BL/6 mice that had received 10 daily subcutaneous doses of PBS, RPMI medium, or B16.F10 TCM into the shoulder. Flow cytometric analysis allowed reporting of the total number of lymph node cells (left), flow cytometry gating scheme, FRCs (right), and FRC Pdpn histogram (furthest right). n ¼ 4 to 9 animals per group. C, qRT-PCR analysis of Pdpn (left), Thy1 (middle), and <t>Il7</t> (right) in FRCs cultured in vitro and treated for 4 days with CCM, B16.F10 TCM, or 4T1 TCM. n ¼ 3 to 10 independent experiments. D, IL7 protein quantification in supernatants of FRCs cultured in vitro and treated with CCM or B16.F10 TCM, assessed by <t>ELISA.</t> n ¼ 4 independent experiments. Flow cytometric quantification of Pdpn protein (E) and Thy1 protein (F) expression for FRCs cultured in vitro and treated with CCM or 4T1 TCM for 4 days. n ¼ 3 independent experiments. G, Flow cytometric analysis of TMRM incorporation by FRCs treated for 4 days with CCM or B16 TCM. n ¼ 4 independent experiments performed in triplicate. H, OCR of FRCs treated with CCM or B16.F10 TCM at baseline and in response to oligomycin, FCCP, and rotenone plus antimycin A. One representative of three experiments with five replicates. I, Baseline OCR from CCM or B16.F10 TCM-treated FRCs. n ¼ 3 experiments with 5 replicates. J, Representative confocal imaging of live cells treated with CCM or B16.F10 TCM. Green, Mitochondria (Mitotracker green); blue, nuclei (Hoechst). Scale bar: 7 mm. Data are mean with SEM. Significance (, P < 0.05; , P < 0.01; , P < 0.001; and , P < 0.0001) was determined by unpaired two-tailed t test or one-way ANOVA with Tukey post hoc (B). s.c., subcutaneous; B16, B16.F10; max, maximum; NS, not significant.
Evos M7000 System, supplied by KU Leuven, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/evos m7000 system/product/KU Leuven
Average 90 stars, based on 1 article reviews
evos m7000 system - by Bioz Stars, 2026-05
90/100 stars
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swift m7000 microscope  (Swift Optical Instruments Inc)
90
Swift Optical Instruments Inc swift m7000 microscope
Figure 1. Soluble factors mediate FRC activation and mitochondrial imbalance. A, Experimental scheme used to investigate tumor-draining factors in vivo. B, Draining lymph nodes were harvested from female C57BL/6 mice that had received 10 daily subcutaneous doses of PBS, RPMI medium, or B16.F10 TCM into the shoulder. Flow cytometric analysis allowed reporting of the total number of lymph node cells (left), flow cytometry gating scheme, FRCs (right), and FRC Pdpn histogram (furthest right). n ¼ 4 to 9 animals per group. C, qRT-PCR analysis of Pdpn (left), Thy1 (middle), and <t>Il7</t> (right) in FRCs cultured in vitro and treated for 4 days with CCM, B16.F10 TCM, or 4T1 TCM. n ¼ 3 to 10 independent experiments. D, IL7 protein quantification in supernatants of FRCs cultured in vitro and treated with CCM or B16.F10 TCM, assessed by <t>ELISA.</t> n ¼ 4 independent experiments. Flow cytometric quantification of Pdpn protein (E) and Thy1 protein (F) expression for FRCs cultured in vitro and treated with CCM or 4T1 TCM for 4 days. n ¼ 3 independent experiments. G, Flow cytometric analysis of TMRM incorporation by FRCs treated for 4 days with CCM or B16 TCM. n ¼ 4 independent experiments performed in triplicate. H, OCR of FRCs treated with CCM or B16.F10 TCM at baseline and in response to oligomycin, FCCP, and rotenone plus antimycin A. One representative of three experiments with five replicates. I, Baseline OCR from CCM or B16.F10 TCM-treated FRCs. n ¼ 3 experiments with 5 replicates. J, Representative confocal imaging of live cells treated with CCM or B16.F10 TCM. Green, Mitochondria (Mitotracker green); blue, nuclei (Hoechst). Scale bar: 7 mm. Data are mean with SEM. Significance (, P < 0.05; , P < 0.01; , P < 0.001; and , P < 0.0001) was determined by unpaired two-tailed t test or one-way ANOVA with Tukey post hoc (B). s.c., subcutaneous; B16, B16.F10; max, maximum; NS, not significant.
Swift M7000 Microscope, supplied by Swift Optical Instruments Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/swift m7000 microscope/product/Swift Optical Instruments Inc
Average 90 stars, based on 1 article reviews
swift m7000 microscope - by Bioz Stars, 2026-05
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medmont m7000 perimeter  (Medmont International Pty Ltd)
90
Medmont International Pty Ltd medmont m7000 perimeter
Figure 1. Soluble factors mediate FRC activation and mitochondrial imbalance. A, Experimental scheme used to investigate tumor-draining factors in vivo. B, Draining lymph nodes were harvested from female C57BL/6 mice that had received 10 daily subcutaneous doses of PBS, RPMI medium, or B16.F10 TCM into the shoulder. Flow cytometric analysis allowed reporting of the total number of lymph node cells (left), flow cytometry gating scheme, FRCs (right), and FRC Pdpn histogram (furthest right). n ¼ 4 to 9 animals per group. C, qRT-PCR analysis of Pdpn (left), Thy1 (middle), and <t>Il7</t> (right) in FRCs cultured in vitro and treated for 4 days with CCM, B16.F10 TCM, or 4T1 TCM. n ¼ 3 to 10 independent experiments. D, IL7 protein quantification in supernatants of FRCs cultured in vitro and treated with CCM or B16.F10 TCM, assessed by <t>ELISA.</t> n ¼ 4 independent experiments. Flow cytometric quantification of Pdpn protein (E) and Thy1 protein (F) expression for FRCs cultured in vitro and treated with CCM or 4T1 TCM for 4 days. n ¼ 3 independent experiments. G, Flow cytometric analysis of TMRM incorporation by FRCs treated for 4 days with CCM or B16 TCM. n ¼ 4 independent experiments performed in triplicate. H, OCR of FRCs treated with CCM or B16.F10 TCM at baseline and in response to oligomycin, FCCP, and rotenone plus antimycin A. One representative of three experiments with five replicates. I, Baseline OCR from CCM or B16.F10 TCM-treated FRCs. n ¼ 3 experiments with 5 replicates. J, Representative confocal imaging of live cells treated with CCM or B16.F10 TCM. Green, Mitochondria (Mitotracker green); blue, nuclei (Hoechst). Scale bar: 7 mm. Data are mean with SEM. Significance (, P < 0.05; , P < 0.01; , P < 0.001; and , P < 0.0001) was determined by unpaired two-tailed t test or one-way ANOVA with Tukey post hoc (B). s.c., subcutaneous; B16, B16.F10; max, maximum; NS, not significant.
Medmont M7000 Perimeter, supplied by Medmont International Pty Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/medmont m7000 perimeter/product/Medmont International Pty Ltd
Average 90 stars, based on 1 article reviews
medmont m7000 perimeter - by Bioz Stars, 2026-05
90/100 stars
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energy dispersive spectroscopy kevex model m7000  (Kevex Inc)
90
Kevex Inc energy dispersive spectroscopy kevex model m7000
Figure 1. Soluble factors mediate FRC activation and mitochondrial imbalance. A, Experimental scheme used to investigate tumor-draining factors in vivo. B, Draining lymph nodes were harvested from female C57BL/6 mice that had received 10 daily subcutaneous doses of PBS, RPMI medium, or B16.F10 TCM into the shoulder. Flow cytometric analysis allowed reporting of the total number of lymph node cells (left), flow cytometry gating scheme, FRCs (right), and FRC Pdpn histogram (furthest right). n ¼ 4 to 9 animals per group. C, qRT-PCR analysis of Pdpn (left), Thy1 (middle), and <t>Il7</t> (right) in FRCs cultured in vitro and treated for 4 days with CCM, B16.F10 TCM, or 4T1 TCM. n ¼ 3 to 10 independent experiments. D, IL7 protein quantification in supernatants of FRCs cultured in vitro and treated with CCM or B16.F10 TCM, assessed by <t>ELISA.</t> n ¼ 4 independent experiments. Flow cytometric quantification of Pdpn protein (E) and Thy1 protein (F) expression for FRCs cultured in vitro and treated with CCM or 4T1 TCM for 4 days. n ¼ 3 independent experiments. G, Flow cytometric analysis of TMRM incorporation by FRCs treated for 4 days with CCM or B16 TCM. n ¼ 4 independent experiments performed in triplicate. H, OCR of FRCs treated with CCM or B16.F10 TCM at baseline and in response to oligomycin, FCCP, and rotenone plus antimycin A. One representative of three experiments with five replicates. I, Baseline OCR from CCM or B16.F10 TCM-treated FRCs. n ¼ 3 experiments with 5 replicates. J, Representative confocal imaging of live cells treated with CCM or B16.F10 TCM. Green, Mitochondria (Mitotracker green); blue, nuclei (Hoechst). Scale bar: 7 mm. Data are mean with SEM. Significance (, P < 0.05; , P < 0.01; , P < 0.001; and , P < 0.0001) was determined by unpaired two-tailed t test or one-way ANOVA with Tukey post hoc (B). s.c., subcutaneous; B16, B16.F10; max, maximum; NS, not significant.
Energy Dispersive Spectroscopy Kevex Model M7000, supplied by Kevex Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/energy dispersive spectroscopy kevex model m7000/product/Kevex Inc
Average 90 stars, based on 1 article reviews
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90/100 stars
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m7000 six-port switching valve  (Rheodyne lp)
90
Rheodyne lp m7000 six-port switching valve
Figure 1. Soluble factors mediate FRC activation and mitochondrial imbalance. A, Experimental scheme used to investigate tumor-draining factors in vivo. B, Draining lymph nodes were harvested from female C57BL/6 mice that had received 10 daily subcutaneous doses of PBS, RPMI medium, or B16.F10 TCM into the shoulder. Flow cytometric analysis allowed reporting of the total number of lymph node cells (left), flow cytometry gating scheme, FRCs (right), and FRC Pdpn histogram (furthest right). n ¼ 4 to 9 animals per group. C, qRT-PCR analysis of Pdpn (left), Thy1 (middle), and <t>Il7</t> (right) in FRCs cultured in vitro and treated for 4 days with CCM, B16.F10 TCM, or 4T1 TCM. n ¼ 3 to 10 independent experiments. D, IL7 protein quantification in supernatants of FRCs cultured in vitro and treated with CCM or B16.F10 TCM, assessed by <t>ELISA.</t> n ¼ 4 independent experiments. Flow cytometric quantification of Pdpn protein (E) and Thy1 protein (F) expression for FRCs cultured in vitro and treated with CCM or 4T1 TCM for 4 days. n ¼ 3 independent experiments. G, Flow cytometric analysis of TMRM incorporation by FRCs treated for 4 days with CCM or B16 TCM. n ¼ 4 independent experiments performed in triplicate. H, OCR of FRCs treated with CCM or B16.F10 TCM at baseline and in response to oligomycin, FCCP, and rotenone plus antimycin A. One representative of three experiments with five replicates. I, Baseline OCR from CCM or B16.F10 TCM-treated FRCs. n ¼ 3 experiments with 5 replicates. J, Representative confocal imaging of live cells treated with CCM or B16.F10 TCM. Green, Mitochondria (Mitotracker green); blue, nuclei (Hoechst). Scale bar: 7 mm. Data are mean with SEM. Significance (, P < 0.05; , P < 0.01; , P < 0.001; and , P < 0.0001) was determined by unpaired two-tailed t test or one-way ANOVA with Tukey post hoc (B). s.c., subcutaneous; B16, B16.F10; max, maximum; NS, not significant.
M7000 Six Port Switching Valve, supplied by Rheodyne lp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
m7000 six-port switching valve - by Bioz Stars, 2026-05
90/100 stars
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evostm m7000 imaging system  (Fisher Scientific)
86
Fisher Scientific evostm m7000 imaging system
Figure 1. Soluble factors mediate FRC activation and mitochondrial imbalance. A, Experimental scheme used to investigate tumor-draining factors in vivo. B, Draining lymph nodes were harvested from female C57BL/6 mice that had received 10 daily subcutaneous doses of PBS, RPMI medium, or B16.F10 TCM into the shoulder. Flow cytometric analysis allowed reporting of the total number of lymph node cells (left), flow cytometry gating scheme, FRCs (right), and FRC Pdpn histogram (furthest right). n ¼ 4 to 9 animals per group. C, qRT-PCR analysis of Pdpn (left), Thy1 (middle), and <t>Il7</t> (right) in FRCs cultured in vitro and treated for 4 days with CCM, B16.F10 TCM, or 4T1 TCM. n ¼ 3 to 10 independent experiments. D, IL7 protein quantification in supernatants of FRCs cultured in vitro and treated with CCM or B16.F10 TCM, assessed by <t>ELISA.</t> n ¼ 4 independent experiments. Flow cytometric quantification of Pdpn protein (E) and Thy1 protein (F) expression for FRCs cultured in vitro and treated with CCM or 4T1 TCM for 4 days. n ¼ 3 independent experiments. G, Flow cytometric analysis of TMRM incorporation by FRCs treated for 4 days with CCM or B16 TCM. n ¼ 4 independent experiments performed in triplicate. H, OCR of FRCs treated with CCM or B16.F10 TCM at baseline and in response to oligomycin, FCCP, and rotenone plus antimycin A. One representative of three experiments with five replicates. I, Baseline OCR from CCM or B16.F10 TCM-treated FRCs. n ¼ 3 experiments with 5 replicates. J, Representative confocal imaging of live cells treated with CCM or B16.F10 TCM. Green, Mitochondria (Mitotracker green); blue, nuclei (Hoechst). Scale bar: 7 mm. Data are mean with SEM. Significance (, P < 0.05; , P < 0.01; , P < 0.001; and , P < 0.0001) was determined by unpaired two-tailed t test or one-way ANOVA with Tukey post hoc (B). s.c., subcutaneous; B16, B16.F10; max, maximum; NS, not significant.
Evostm M7000 Imaging System, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/evostm m7000 imaging system/product/Fisher Scientific
Average 86 stars, based on 1 article reviews
evostm m7000 imaging system - by Bioz Stars, 2026-05
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Image Search Results


Figure 1. Soluble factors mediate FRC activation and mitochondrial imbalance. A, Experimental scheme used to investigate tumor-draining factors in vivo. B, Draining lymph nodes were harvested from female C57BL/6 mice that had received 10 daily subcutaneous doses of PBS, RPMI medium, or B16.F10 TCM into the shoulder. Flow cytometric analysis allowed reporting of the total number of lymph node cells (left), flow cytometry gating scheme, FRCs (right), and FRC Pdpn histogram (furthest right). n ¼ 4 to 9 animals per group. C, qRT-PCR analysis of Pdpn (left), Thy1 (middle), and Il7 (right) in FRCs cultured in vitro and treated for 4 days with CCM, B16.F10 TCM, or 4T1 TCM. n ¼ 3 to 10 independent experiments. D, IL7 protein quantification in supernatants of FRCs cultured in vitro and treated with CCM or B16.F10 TCM, assessed by ELISA. n ¼ 4 independent experiments. Flow cytometric quantification of Pdpn protein (E) and Thy1 protein (F) expression for FRCs cultured in vitro and treated with CCM or 4T1 TCM for 4 days. n ¼ 3 independent experiments. G, Flow cytometric analysis of TMRM incorporation by FRCs treated for 4 days with CCM or B16 TCM. n ¼ 4 independent experiments performed in triplicate. H, OCR of FRCs treated with CCM or B16.F10 TCM at baseline and in response to oligomycin, FCCP, and rotenone plus antimycin A. One representative of three experiments with five replicates. I, Baseline OCR from CCM or B16.F10 TCM-treated FRCs. n ¼ 3 experiments with 5 replicates. J, Representative confocal imaging of live cells treated with CCM or B16.F10 TCM. Green, Mitochondria (Mitotracker green); blue, nuclei (Hoechst). Scale bar: 7 mm. Data are mean with SEM. Significance (, P < 0.05; , P < 0.01; , P < 0.001; and , P < 0.0001) was determined by unpaired two-tailed t test or one-way ANOVA with Tukey post hoc (B). s.c., subcutaneous; B16, B16.F10; max, maximum; NS, not significant.

Journal: Cancer Immunology Research

Article Title: Tumor-Derived Lactic Acid Modulates Activation and Metabolic Status of Draining Lymph Node Stroma

doi: 10.1158/2326-6066.cir-21-0778

Figure Lengend Snippet: Figure 1. Soluble factors mediate FRC activation and mitochondrial imbalance. A, Experimental scheme used to investigate tumor-draining factors in vivo. B, Draining lymph nodes were harvested from female C57BL/6 mice that had received 10 daily subcutaneous doses of PBS, RPMI medium, or B16.F10 TCM into the shoulder. Flow cytometric analysis allowed reporting of the total number of lymph node cells (left), flow cytometry gating scheme, FRCs (right), and FRC Pdpn histogram (furthest right). n ¼ 4 to 9 animals per group. C, qRT-PCR analysis of Pdpn (left), Thy1 (middle), and Il7 (right) in FRCs cultured in vitro and treated for 4 days with CCM, B16.F10 TCM, or 4T1 TCM. n ¼ 3 to 10 independent experiments. D, IL7 protein quantification in supernatants of FRCs cultured in vitro and treated with CCM or B16.F10 TCM, assessed by ELISA. n ¼ 4 independent experiments. Flow cytometric quantification of Pdpn protein (E) and Thy1 protein (F) expression for FRCs cultured in vitro and treated with CCM or 4T1 TCM for 4 days. n ¼ 3 independent experiments. G, Flow cytometric analysis of TMRM incorporation by FRCs treated for 4 days with CCM or B16 TCM. n ¼ 4 independent experiments performed in triplicate. H, OCR of FRCs treated with CCM or B16.F10 TCM at baseline and in response to oligomycin, FCCP, and rotenone plus antimycin A. One representative of three experiments with five replicates. I, Baseline OCR from CCM or B16.F10 TCM-treated FRCs. n ¼ 3 experiments with 5 replicates. J, Representative confocal imaging of live cells treated with CCM or B16.F10 TCM. Green, Mitochondria (Mitotracker green); blue, nuclei (Hoechst). Scale bar: 7 mm. Data are mean with SEM. Significance (, P < 0.05; , P < 0.01; , P < 0.001; and , P < 0.0001) was determined by unpaired two-tailed t test or one-way ANOVA with Tukey post hoc (B). s.c., subcutaneous; B16, B16.F10; max, maximum; NS, not significant.

Article Snippet: Protein quantification per ELISA was performed according to the R&D System Quantikine IL7 ELISA Kit (R&D Systems, #M70000,) product manual.

Techniques: Activation Assay, In Vivo, Cytometry, Quantitative RT-PCR, Cell Culture, In Vitro, Enzyme-linked Immunosorbent Assay, Expressing, Imaging, Two Tailed Test