Journal: Cell

Article Title: SARS-CoV-2 mRNA vaccination induces functionally diverse antibodies to NTD, RBD, and S2

doi: 10.1016/j.cell.2021.06.005

Figure Lengend Snippet:

Article Snippet: M13 Reverse (−27) , Integrated DNA Technologies , 51-01-13-03.

Techniques: Virus, Recombinant, Lysis, Reverse Transcription, Amplification, Variant Assay, Nested PCR, Cloning, Plasmid Preparation, Software

Journal: Microbes and environments

Article Title: A New Fractionation and Recovery Method of Viral Genomes Based on Nucleic Acid Composition and Structure Using Tandem Column Chromatography

doi: 10.1264/jsme2.me14174

Figure Lengend Snippet: Fig. 2. Electrophoretic analysis of fractionated viral nucleic acids. (A) Binding and elution conditions for viral ssDNA on a cellulose column. Fifty nanograms of M13 circular ssDNA was adjusted to 1×STE buffer containing 25% ethanol, and applied to a cellulose spin-column. Lanes 1, 2, and 3 show the flow-through with 1×STE buffer containing 25% ethanol, the fraction eluted with 1×STE containing 15% ethanol, and the fraction eluted with 1×STE, respectively. (B) Separation of four known viral nucleic acid types. In the electropherogram, each of the lanes shows 50 ng of lambda dsDNA (lane 1), M13 circular ssDNA (lane 2), MoCV1-A dsRNA (lane 4), and 150 ng of MS2 ssRNA (lane 3), a mix- ture of the four viral genomes (50 ng of dsDNA, ssDNA, and dsRNA and 150 ng of ssRNA) (lane 5), the elution fraction of 1.0 M phosphate buffer for a hydroxyapatite column (lane 6), the flowthrough fraction of 25% ethanol in 1×STE buffer of cellulose spin column (lane 7), an elu- tion fraction of 1×STE buffer containing 15% ethanol (lane 8), and an elution fraction eluted with 1×STE (lane 9). (C) Fractionation of envi- ronmental viral nucleic acids from 100 mL of coastal sea water from the quay in JAMSTEC (35°19’9’’N, 139°39’2’’E). Lane 1, the elution fraction of 1.0 M phosphate buffer for a hydroxyapatite column; lane 2, the flow through fraction of 25% ethanol in 1×STE buffer of cellulose spin col- umn; lane 3, an elution fraction of 1×STE buffer containing 15% ethanol; and lane 4, an elution fraction eluted with 1×STE. Nucleic acids were sepa- rated by agarose gel (1%) electrophoresis and visualized with SYBR Gold.

Article Snippet: Escherichia coli phage lambda (NBRC20016) and M13 (DSM 13976) and their hosts (NBRC12713 and NBRC3302) were obtained from NBRC (Kisarazu, Japan) and DSMZ (Braunschweig, Germany).

Techniques: Binding Assay, Fractionation, Agarose Gel Electrophoresis, Electrophoresis

Journal: Cell reports

Article Title: Phage-delivered CRISPR-Cas9 for strain-specific depletion and genomic deletions in the gut microbiome

doi: 10.1016/j.celrep.2021.109930

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Plasmid: HP4_M13 (helper plasmid, Km R ) , , RRID: Addgene_120340.

Techniques: Virus, Cloning, Recombinant, Library Quantification, Ligation, Sequencing, CRISPR, Plasmid Preparation, Expressing, Software