Journal: Nature communications

Article Title: The HIV co-receptor CCR5 regulates osteoclast function.

doi: 10.1038/s41467-017-02368-5

Figure Lengend Snippet: Fig. 7 The blockade of CCL5 in vivo. a The serum levels of CCL3 and CCL5 in 10-week-old mice were measured by an ELISA (n = 5). b Mouse anti-CCL5 neutralizing antibodies (mCCL5 neu ab, 500 μg per mouse) were injected (once per week for 2 weeks) into 6-week-old male C57BL/6J mice (n = 5). IgG was administered to the control group. c, d μCT images (scale bars, 100 μm) and parameters (n = 5 mice per group) are shown. e Representative images of the distal femurs from the control IgG and mCCL5 ab groups. HE- (scale bars, 100 μm) and TRAP-stained sections (scale bars, 50 μm) are shown in the upper and lower panels, respectively (n = 5). f Quantitative bone histomorphometric analyses were conducted of the trabecular bones in the distal femurs of control IgG and mouse CCL5 neutralizing antibody-injected mice. *P < 0.05 (by Student’s t-test) in comparison to control and mCCL5-neuAb mice. All values are shown as the mean ± SD, n = 5

Article Snippet: A Mouse RANKL ELISA kit (R&D system), Mouse Osteocalcin/Bone gla protein, OT/BGP ELISA kit (Biomedical Technologies, Stoughton, MA), MCSF (R&D system), Osteoprotegerin/TNFRSF11B ELISA kit (R&D system).

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Injection, Control, Staining, Comparison, Neutralizing Assay