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  • 97
    Millipore m tris hcl
    M Tris Hcl, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris hcl/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m tris hcl - by Bioz Stars, 2021-05
    97/100 stars
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    86
    Roche m tris hcl
    SDS–PAGE gels [12.5%( w / v ) acrylamide; coloured by the Coomassie staining technique] showing purification of the holo-His 6 <t>-HasA–HasR</t> complex. Lane 1, solubilized membrane fraction in 100 m M <t>Tris–HCl</t> pH 7.5, 2%( w / v ) ZW3-14;
    M Tris Hcl, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris hcl/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m tris hcl - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    99
    Millipore m tris
    SDS–PAGE gels [12.5%( w / v ) acrylamide; coloured by the Coomassie staining technique] showing purification of the holo-His 6 <t>-HasA–HasR</t> complex. Lane 1, solubilized membrane fraction in 100 m M <t>Tris–HCl</t> pH 7.5, 2%( w / v ) ZW3-14;
    M Tris, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m tris - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    86
    GE Healthcare m tris hcl
    Detection of <t>ZmIPK1</t> protein in different maize tissues via western blotting. Proteins were extracted from maize leaf (lane 1), immature ear (lane 2), root (lane 3), seed at 12 DAP (lane 4), endosperm at 15 (lane 5), 20 (lane 6), and 25 (lane 7) DAP, and embryo at 24 (lane 8) and 31 (lane 9) DAP. After SDS-PAGE on a 8% to 16% gradient <t>Tris-Gly</t> polyacrylamide gel, the proteins were transferred onto a nitrocellulose membrane, incubated with polyclonal antibodies against maize ZmIPK1 protein, and detected with goat anti-rabbit antibody conjugated to horseradish peroxidase. ZmIPK1 protein (10 ng) purified from E. coli lysate was used as the reference (lane 10).
    M Tris Hcl, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris hcl/product/GE Healthcare
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m tris hcl - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier





    Image Search Results


    SDS–PAGE gels [12.5%( w / v ) acrylamide; coloured by the Coomassie staining technique] showing purification of the holo-His 6 -HasA–HasR complex. Lane 1, solubilized membrane fraction in 100 m M Tris–HCl pH 7.5, 2%( w / v ) ZW3-14;

    Journal: Acta Crystallographica Section F: Structural Biology and Crystallization Communications

    Article Title: Purification, crystallization and preliminary X-ray analysis of the outer membrane complex HasA-HasR from Serratia marcescens

    doi: 10.1107/S1744309105041394

    Figure Lengend Snippet: SDS–PAGE gels [12.5%( w / v ) acrylamide; coloured by the Coomassie staining technique] showing purification of the holo-His 6 -HasA–HasR complex. Lane 1, solubilized membrane fraction in 100 m M Tris–HCl pH 7.5, 2%( w / v ) ZW3-14;

    Article Snippet: The cells were harvested at 5000 rev min−1 for 10 min and resuspended in 10 m M Tris–HCl pH 7.5, 10 m M imidazole for His6 -HasA cells and 100 m M Tris–HCl pH 7.5 for HasR cells with protease-inhibitor cocktail (Roche; one tablet per 50 ml).

    Techniques: SDS Page, Staining, Purification

    Detection of ZmIPK1 protein in different maize tissues via western blotting. Proteins were extracted from maize leaf (lane 1), immature ear (lane 2), root (lane 3), seed at 12 DAP (lane 4), endosperm at 15 (lane 5), 20 (lane 6), and 25 (lane 7) DAP, and embryo at 24 (lane 8) and 31 (lane 9) DAP. After SDS-PAGE on a 8% to 16% gradient Tris-Gly polyacrylamide gel, the proteins were transferred onto a nitrocellulose membrane, incubated with polyclonal antibodies against maize ZmIPK1 protein, and detected with goat anti-rabbit antibody conjugated to horseradish peroxidase. ZmIPK1 protein (10 ng) purified from E. coli lysate was used as the reference (lane 10).

    Journal: Plant Physiology

    Article Title: Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase from Maize: Molecular and Biochemical Characterization [OA]

    doi: 10.1104/pp.107.095455

    Figure Lengend Snippet: Detection of ZmIPK1 protein in different maize tissues via western blotting. Proteins were extracted from maize leaf (lane 1), immature ear (lane 2), root (lane 3), seed at 12 DAP (lane 4), endosperm at 15 (lane 5), 20 (lane 6), and 25 (lane 7) DAP, and embryo at 24 (lane 8) and 31 (lane 9) DAP. After SDS-PAGE on a 8% to 16% gradient Tris-Gly polyacrylamide gel, the proteins were transferred onto a nitrocellulose membrane, incubated with polyclonal antibodies against maize ZmIPK1 protein, and detected with goat anti-rabbit antibody conjugated to horseradish peroxidase. ZmIPK1 protein (10 ng) purified from E. coli lysate was used as the reference (lane 10).

    Article Snippet: The unbound fractions containing ZmIPK1 protein were pooled and dialyzed against 20 m m Tris-HCl, pH 7.5, 1 m m DTT at room temperature for 2 h. Then, the sample was applied to an 8 mL Mono Q column (Amersham).

    Techniques: Western Blot, SDS Page, Incubation, Purification