m tris Search Results


m tris hcl ph8 0  (Jena Bioscience)
92
Jena Bioscience m tris hcl ph8 0
M Tris Hcl Ph8 0, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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auranofin  (Chem Impex International)
95
Chem Impex International auranofin
IL-8 level was assessed in Caco-2 cells treated with supernatant containing C. difficile toxins, with or without the addition of <t>auranofin.</t> OD450 coincides with the level of IL-8 in the cell supernatant. Error bars represent standard deviation values from triplicate samples used for each test agent. An asterisk (*) indicates significant difference (P < 0.05) between cells exposed to supernatant containing toxin alone and cells treated with supernatant containing auranofin (1 µg/mL) using one-way (ANOVA) followed by Dunnett’s multiple comparisons test.
Auranofin, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris hcl  (Jena Bioscience)
94
Jena Bioscience tris hcl
IL-8 level was assessed in Caco-2 cells treated with supernatant containing C. difficile toxins, with or without the addition of <t>auranofin.</t> OD450 coincides with the level of IL-8 in the cell supernatant. Error bars represent standard deviation values from triplicate samples used for each test agent. An asterisk (*) indicates significant difference (P < 0.05) between cells exposed to supernatant containing toxin alone and cells treated with supernatant containing auranofin (1 µg/mL) using one-way (ANOVA) followed by Dunnett’s multiple comparisons test.
Tris Hcl, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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chondroitinase abc solution  (AMS Biotechnology)
96
AMS Biotechnology chondroitinase abc solution
IL-8 level was assessed in Caco-2 cells treated with supernatant containing C. difficile toxins, with or without the addition of <t>auranofin.</t> OD450 coincides with the level of IL-8 in the cell supernatant. Error bars represent standard deviation values from triplicate samples used for each test agent. An asterisk (*) indicates significant difference (P < 0.05) between cells exposed to supernatant containing toxin alone and cells treated with supernatant containing auranofin (1 µg/mL) using one-way (ANOVA) followed by Dunnett’s multiple comparisons test.
Chondroitinase Abc Solution, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris hcl  (Chem Impex International)
95
Chem Impex International tris hcl
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Tris Hcl, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tris hcl/product/Chem Impex International
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tris buffer  (Jena Bioscience)
92
Jena Bioscience tris buffer
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Tris Buffer, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris  (Chem Impex International)
95
Chem Impex International tris
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Tris, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tris/product/Chem Impex International
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tris - by Bioz Stars, 2026-04
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dess martin periodinane  (Chem Impex International)
95
Chem Impex International dess martin periodinane
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Dess Martin Periodinane, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spheroplasting media without lys  (AMS Biotechnology)
96
AMS Biotechnology spheroplasting media without lys
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Spheroplasting Media Without Lys, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bis-tris-hcl 1 m solution  (Rigaku Corporation)
90
Rigaku Corporation bis-tris-hcl 1 m solution
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Bis Tris Hcl 1 M Solution, supplied by Rigaku Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4 × laemmli sample buffer  (Schleicher Inc)
90
Schleicher Inc 4 × laemmli sample buffer
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
4 × Laemmli Sample Buffer, supplied by Schleicher Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1m tris-hcl ph6.8  (Beyotime)
90
Beyotime 1m tris-hcl ph6.8

1m Tris Hcl Ph6.8, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


IL-8 level was assessed in Caco-2 cells treated with supernatant containing C. difficile toxins, with or without the addition of auranofin. OD450 coincides with the level of IL-8 in the cell supernatant. Error bars represent standard deviation values from triplicate samples used for each test agent. An asterisk (*) indicates significant difference (P < 0.05) between cells exposed to supernatant containing toxin alone and cells treated with supernatant containing auranofin (1 µg/mL) using one-way (ANOVA) followed by Dunnett’s multiple comparisons test.

Journal: International journal of antimicrobial agents

Article Title: Antibacterial and antivirulence activities of auranofin against Clostridium difficile

doi: 10.1016/j.ijantimicag.2018.09.018

Figure Lengend Snippet: IL-8 level was assessed in Caco-2 cells treated with supernatant containing C. difficile toxins, with or without the addition of auranofin. OD450 coincides with the level of IL-8 in the cell supernatant. Error bars represent standard deviation values from triplicate samples used for each test agent. An asterisk (*) indicates significant difference (P < 0.05) between cells exposed to supernatant containing toxin alone and cells treated with supernatant containing auranofin (1 µg/mL) using one-way (ANOVA) followed by Dunnett’s multiple comparisons test.

Article Snippet: Auranofin, linezolid (Chem-Impex International, Wood Dale, IL), vancomycin hydrochloride (Gold Biotechnology, St. Louis, MO) metronidazole (Beantown Chemical Corporation, Hudson, NH), sodium selenite (MP Biomedicals, Santa Ana, CA), and fidaxomicin (Apexbio, Houston, TX) were procured from commercial vendors.

Techniques: Standard Deviation

The minimum inhibitory concentration (MIC, µg/mL) of auranofin and control drugs against vancomycin-resistant Enterococcus faecium isolates.

Journal: International journal of antimicrobial agents

Article Title: Antibacterial and antivirulence activities of auranofin against Clostridium difficile

doi: 10.1016/j.ijantimicag.2018.09.018

Figure Lengend Snippet: The minimum inhibitory concentration (MIC, µg/mL) of auranofin and control drugs against vancomycin-resistant Enterococcus faecium isolates.

Article Snippet: Auranofin, linezolid (Chem-Impex International, Wood Dale, IL), vancomycin hydrochloride (Gold Biotechnology, St. Louis, MO) metronidazole (Beantown Chemical Corporation, Hudson, NH), sodium selenite (MP Biomedicals, Santa Ana, CA), and fidaxomicin (Apexbio, Houston, TX) were procured from commercial vendors.

Techniques: Concentration Assay

The minimum inhibitory concentration (MIC, µg/mL) of auranofin and control anticlostridial drugs against C. difficile isolates.

Journal: International journal of antimicrobial agents

Article Title: Antibacterial and antivirulence activities of auranofin against Clostridium difficile

doi: 10.1016/j.ijantimicag.2018.09.018

Figure Lengend Snippet: The minimum inhibitory concentration (MIC, µg/mL) of auranofin and control anticlostridial drugs against C. difficile isolates.

Article Snippet: Auranofin, linezolid (Chem-Impex International, Wood Dale, IL), vancomycin hydrochloride (Gold Biotechnology, St. Louis, MO) metronidazole (Beantown Chemical Corporation, Hudson, NH), sodium selenite (MP Biomedicals, Santa Ana, CA), and fidaxomicin (Apexbio, Houston, TX) were procured from commercial vendors.

Techniques: Concentration Assay

Northern blot analyses of intracellular aminoacylation of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM Tris, pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.

Journal: ACS synthetic biology

Article Title: Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli

doi: 10.1021/acssynbio.5b00197

Figure Lengend Snippet: Northern blot analyses of intracellular aminoacylation of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM Tris, pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.

Article Snippet: Biochemical Characterization of the IFRS/tRNA Pyl Pair The aminoacylation assays were carried out for 60 min at 37 °C in aminoacylation buffer containing 100 mM Tris-HCl (pH 7.0), 10 mM MgCl 2 , 50 mM potassium chloride, and 5 mM DTT, with 1 mM 3-I-Phe (ChemImpex), 2 mM ATP, 500 nM IFRS, and 5 µM 32 P 3′-end-labeled transcript.

Techniques: Northern Blot, Expressing, Plasmid Preparation

Journal: STAR Protocols

Article Title: A metabolic labeling protocol to enrich myristoylated proteins from Caenorhabditis elegans

doi: 10.1016/j.xpro.2021.101013

Figure Lengend Snippet:

Article Snippet: 1M Tris-HCL PH6.8 , Beyotime , Cat# ST768.

Techniques: Recombinant, Virus, Protein Enrichment, Staining, Software