m dtt Search Results


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  • 99
    New England Biolabs m dtt
    M Dtt, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher m dtt
    Polysulfides inhibit PTEN by sulfane sulfur addition. (A) Recombinant PTEN-WT was <t>prereduced</t> with 20 m M <t>DTT</t> for 10 min, followed by buffer exchange into a nonreducing PTEN assay buffer and exposure to 100 μ M H 2 O 2 or NaHS
    M Dtt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore m dtt
    Redox titration of ZmGLYK activity. Recombinant ZmGLYK was incubated with varying ratios of <t>DTT</t> ox /DTT and 0.9 μ m <t>EcoTrx</t> at pH 7.7 and assayed for activity. Data points represent means from three independent experiments. The redox midpoint potential
    M Dtt, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    GE Healthcare m dtt
    Disulfide reduction during catalysis by Ero1p and the de-regulated mutant C150A/C295A. Enzyme at a final concentration of 2 μ M was mixed with 100 μ M <t>Trx</t> red . Aliquots were removed from the reactions at the indicated time points, and disulfide status was preserved by blocking with maleimide reagents. The migration of Ero1p on SDS-PAGE at the various time points was examined and compared with wild-type Ero1p (WT), the indicated Ero1p mutants, and <t>DTT-treated</t> Ero1p (WT reduced). (A) The Trx red oxidation reaction was blocked with AMS and separated by SDS-PAGE under nonreducing conditions. The asterisk (*) indicates a species that migrates slower than Ero1p C90A/C349A when blocked with AMS, but faster than this disulfide mutant when blocked with NEM (compare with panel C). (B) The same experiment as in (A) was performed, but the samples were reduced with DTT after AMS treatment before separation by SDS-PAGE. (C) Reactions were blocked with NEM and applied to the gel under nonreducing conditions.
    M Dtt, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 2052 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 340 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 628 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore m dtt dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Boehringer Mannheim m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Carl Roth GmbH m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 92/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Research Products International m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by Research Products International, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Boehringer Mannheim 10ã‚â m m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    10ã‚â M M Dtt, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Fisher Scientific 4ãƒâ— ssc 1 m m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    4ãƒâ— Ssc 1 M M Dtt, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    FUJIFILM m dtt pbs
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt Pbs, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m dtt  (Abcam)
    99
    Abcam m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m dtt  (ATUM)
    90
    ATUM m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by ATUM, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Bio Basic Canada m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Duchefa m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by Duchefa, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Gold Biotechnology Inc m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    INDOFINE m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by INDOFINE, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Nacalai m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m dtt  (Sanyo)
    92
    Sanyo m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by Sanyo, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Trion Research m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by Trion Research, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Polysulfides inhibit PTEN by sulfane sulfur addition. (A) Recombinant PTEN-WT was prereduced with 20 m M DTT for 10 min, followed by buffer exchange into a nonreducing PTEN assay buffer and exposure to 100 μ M H 2 O 2 or NaHS

    Journal: Antioxidants & Redox Signaling

    Article Title: Polysulfides Link H2S to Protein Thiol Oxidation

    doi: 10.1089/ars.2012.5041

    Figure Lengend Snippet: Polysulfides inhibit PTEN by sulfane sulfur addition. (A) Recombinant PTEN-WT was prereduced with 20 m M DTT for 10 min, followed by buffer exchange into a nonreducing PTEN assay buffer and exposure to 100 μ M H 2 O 2 or NaHS

    Article Snippet: Typically, roGFP2-His was prereduced with 20 m M DTT for 10 min before the experiment, and passed through gel filtration columns (Thermo Scientific) pre-equilibrated with the roGFP2 assay buffer to remove DTT immediately before the start of the assay.

    Techniques: Recombinant, Buffer Exchange

    Redox titration of ZmGLYK activity. Recombinant ZmGLYK was incubated with varying ratios of DTT ox /DTT and 0.9 μ m EcoTrx at pH 7.7 and assayed for activity. Data points represent means from three independent experiments. The redox midpoint potential

    Journal: Plant Physiology

    Article Title: An Autoinhibitory Domain Confers Redox Regulation to Maize Glycerate Kinase 1An Autoinhibitory Domain Confers Redox Regulation to Maize Glycerate Kinase 1 [W]

    doi: 10.1104/pp.110.157719

    Figure Lengend Snippet: Redox titration of ZmGLYK activity. Recombinant ZmGLYK was incubated with varying ratios of DTT ox /DTT and 0.9 μ m EcoTrx at pH 7.7 and assayed for activity. Data points represent means from three independent experiments. The redox midpoint potential

    Article Snippet: For reduction, about 0.9 μ m purified GLYK protein was incubated with 10 m m DTT and 0.9 μ m EcoTrx (Sigma-Aldrich) in 20 m m sodium phosphate, pH 7.8, for 60 min at room temperature.

    Techniques: Titration, Activity Assay, Recombinant, Incubation

    Light and thiol activation of GLYK in desalted maize leaf soluble crude extracts. A, Light activation after 4 h of dark adaptation of leaves. After 10 min of illumination, leaves were darkened again. B, Thiol activation by addition of 10 m m DTT to extracts

    Journal: Plant Physiology

    Article Title: An Autoinhibitory Domain Confers Redox Regulation to Maize Glycerate Kinase 1An Autoinhibitory Domain Confers Redox Regulation to Maize Glycerate Kinase 1 [W]

    doi: 10.1104/pp.110.157719

    Figure Lengend Snippet: Light and thiol activation of GLYK in desalted maize leaf soluble crude extracts. A, Light activation after 4 h of dark adaptation of leaves. After 10 min of illumination, leaves were darkened again. B, Thiol activation by addition of 10 m m DTT to extracts

    Article Snippet: For reduction, about 0.9 μ m purified GLYK protein was incubated with 10 m m DTT and 0.9 μ m EcoTrx (Sigma-Aldrich) in 20 m m sodium phosphate, pH 7.8, for 60 min at room temperature.

    Techniques: Activation Assay

    Disulfide reduction during catalysis by Ero1p and the de-regulated mutant C150A/C295A. Enzyme at a final concentration of 2 μ M was mixed with 100 μ M Trx red . Aliquots were removed from the reactions at the indicated time points, and disulfide status was preserved by blocking with maleimide reagents. The migration of Ero1p on SDS-PAGE at the various time points was examined and compared with wild-type Ero1p (WT), the indicated Ero1p mutants, and DTT-treated Ero1p (WT reduced). (A) The Trx red oxidation reaction was blocked with AMS and separated by SDS-PAGE under nonreducing conditions. The asterisk (*) indicates a species that migrates slower than Ero1p C90A/C349A when blocked with AMS, but faster than this disulfide mutant when blocked with NEM (compare with panel C). (B) The same experiment as in (A) was performed, but the samples were reduced with DTT after AMS treatment before separation by SDS-PAGE. (C) Reactions were blocked with NEM and applied to the gel under nonreducing conditions.

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Steps in reductive activation of the disulfide-generating enzyme Ero1p

    doi: 10.1002/pro.473

    Figure Lengend Snippet: Disulfide reduction during catalysis by Ero1p and the de-regulated mutant C150A/C295A. Enzyme at a final concentration of 2 μ M was mixed with 100 μ M Trx red . Aliquots were removed from the reactions at the indicated time points, and disulfide status was preserved by blocking with maleimide reagents. The migration of Ero1p on SDS-PAGE at the various time points was examined and compared with wild-type Ero1p (WT), the indicated Ero1p mutants, and DTT-treated Ero1p (WT reduced). (A) The Trx red oxidation reaction was blocked with AMS and separated by SDS-PAGE under nonreducing conditions. The asterisk (*) indicates a species that migrates slower than Ero1p C90A/C349A when blocked with AMS, but faster than this disulfide mutant when blocked with NEM (compare with panel C). (B) The same experiment as in (A) was performed, but the samples were reduced with DTT after AMS treatment before separation by SDS-PAGE. (C) Reactions were blocked with NEM and applied to the gel under nonreducing conditions.

    Article Snippet: For oxidation assays, Trx was reduced with 100 m M DTT for 1 h and then desalted using a PD-10 column (GE Healthcare) equilibrated in 50 m M phosphate buffer, pH 7.5, 65 m M NaCl, 0.5 m M EDTA.

    Techniques: Mutagenesis, Concentration Assay, Blocking Assay, Migration, SDS Page, Affinity Magnetic Separation

    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m DTT with apo-AcnB (0.2

    Journal: The Journal of Biological Chemistry

    Article Title: Iron-Sulfur (Fe-S) Cluster Assembly

    doi: 10.1074/jbc.M110.127449

    Figure Lengend Snippet: Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m DTT with apo-AcnB (0.2

    Article Snippet: For the experiment in the absence of DTT during the FeS transfer, apo-aconitase B was first pretreated with 5 m m DTT for 30 min, before desalting the protein solution via a MicroBiospin column (Bio-Rad).

    Techniques: Incubation