Journal: Biology of Sex Differences

Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

doi: 10.1186/s13293-018-0164-z

Figure Lengend Snippet: List of primary antibodies

Article Snippet: Mitoprofile total OXPHOS , Total Extracts DRG , 1:1.000 , Abcam (ab10413).

Techniques: Isolation

Journal: Biology of Sex Differences

Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

doi: 10.1186/s13293-018-0164-z

Figure Lengend Snippet: Effect of short-term diabetes on PGC-1α gene expression, respiratory chain complex IV expression and mitochondrial DNA. a RNA was extracted from DRG of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. RT-PCR was used to analyze mRNA levels of PGC1-α. b Immunoblot of mitochondrial OXPHOS Complex IV from DRG total extracts of Ctrl and STZ M and F rats. Representative Western blots are presented above the graph, with the respective loading control (GAPDH). The densitometry of each band was analyzed, and the results are expressed as percentage of M Ctrl. c Total DNA was isolated from non-diabetic Ctrl and STZ rats and run for quantitative real-time PCR to obtain a relative ratio of mt-CoxII (a gene coded on the mitochondrial genome) over 36B4 (a gene coded on nuclear genome), as indicator for relative mt-DNA copy number. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sexby diabetes were analyzed using two-way ANOVA (significance: ## p < 0.01; ### p < 0.001) followed by Bonferroni post hoc test (significance: * p < 0.05; **p < 0.01)

Article Snippet: Mitoprofile total OXPHOS , Total Extracts DRG , 1:1.000 , Abcam (ab10413).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Isolation, Real-time Polymerase Chain Reaction

Journal: Pharmaceuticals

Article Title: Daphnetin Ameliorates Neuropathic Pain via Regulation of Microglial Responses and Glycerophospholipid Metabolism in the Spinal Cord

doi: 10.3390/ph17060789

Figure Lengend Snippet: Effect of DAP on pain sensation in CCI rats. ( A ) Structural formula of DAP. ( B ) Experimental flowchart. ( C ) Spinal cord subdural intubation in rats. The rats were intubated at the L4−L5 spinal cord in the subdural cavity, as shown in the figure. After the injection of lidocaine hydrochloride, they immediately became paralyzed. ( D ) Change in TWT in CCI rats at different administration times ( n = 6). ( E ) Change in MWT in CCI rats at different administration times ( n = 6). * p < 0.05, ** p < 0.01 vs. model group.

Article Snippet: In experiment 1, rats were divided into 6 groups ( n = 6) in the pharmacodynamic study to analyze the mechanism of DAP activity: the control, sham, CCI, positive (3 mg/mL morphine) (850807, Qinghai pharmaceutical factory, Qinghai, China), DAP high-dose (DAPH; 0.5 mg/mL) (B21084, Yuanye Biotechnology Co., Ltd., Shanghai, China), and DAP low-dose (DAPL; 0.2 mg/mL) groups.

Techniques: Injection

Journal: Pharmaceuticals

Article Title: Daphnetin Ameliorates Neuropathic Pain via Regulation of Microglial Responses and Glycerophospholipid Metabolism in the Spinal Cord

doi: 10.3390/ph17060789

Figure Lengend Snippet: Effect of DAP on microglia activation and expression of inflammatory factors in the spinal cords of CCI rats. ( A ) The rat spinal cord microglia marker Iba-1 (green) was stained with immunofluorescence. The scale bar is 20 μm ( n = 3). ( B – E ) Expression of IL-1β, IL-6, and TNF-α proteins in the spinal cord ( n = 3). ( F ) Fluorescence intensity of Iba-1 in immunofluorescence. * p < 0.05, ** p < 0.01 vs. model group.

Article Snippet: In experiment 1, rats were divided into 6 groups ( n = 6) in the pharmacodynamic study to analyze the mechanism of DAP activity: the control, sham, CCI, positive (3 mg/mL morphine) (850807, Qinghai pharmaceutical factory, Qinghai, China), DAP high-dose (DAPH; 0.5 mg/mL) (B21084, Yuanye Biotechnology Co., Ltd., Shanghai, China), and DAP low-dose (DAPL; 0.2 mg/mL) groups.

Techniques: Activation Assay, Expressing, Marker, Staining, Immunofluorescence, Fluorescence

Journal: Pharmaceuticals

Article Title: Daphnetin Ameliorates Neuropathic Pain via Regulation of Microglial Responses and Glycerophospholipid Metabolism in the Spinal Cord

doi: 10.3390/ph17060789

Figure Lengend Snippet: Effect of DAP on P2X 4 receptors in spinal microglia in CCI rats. ( A – D , F , G ) Expression of IRF8, IRF5, P2X 4 , and BDNF proteins in spinal cord samples ( n = 3). ( E , H , I ) Expression of Iba-1 (red) and P2X 4 (green) receptors in the spinal dorsal horn, as determined by immunofluorescence double staining. The scale bar is 20 μm ( n = 3). * p < 0.05, ** p < 0.01 vs. model group.

Article Snippet: In experiment 1, rats were divided into 6 groups ( n = 6) in the pharmacodynamic study to analyze the mechanism of DAP activity: the control, sham, CCI, positive (3 mg/mL morphine) (850807, Qinghai pharmaceutical factory, Qinghai, China), DAP high-dose (DAPH; 0.5 mg/mL) (B21084, Yuanye Biotechnology Co., Ltd., Shanghai, China), and DAP low-dose (DAPL; 0.2 mg/mL) groups.

Techniques: Expressing, Immunofluorescence, Double Staining

Journal: Pharmaceuticals

Article Title: Daphnetin Ameliorates Neuropathic Pain via Regulation of Microglial Responses and Glycerophospholipid Metabolism in the Spinal Cord

doi: 10.3390/ph17060789

Figure Lengend Snippet: Effect of DAP on MAPK-pathway activation in the spinal microglia of CCI rats. ( A , B , D , E ) The expression of P38/p-P38, JNK/p-JNK, and ERK1/2/p-ERK1/2 proteins in the spinal cord ( n = 3). ( C , F , G ) The expression of Iba-1 and P38 in the spinal dorsal horn in immunofluorescence double staining. The scale bar is 20 μm ( n = 3). * p < 0.05, ** p < 0.01 vs. model group.

Article Snippet: In experiment 1, rats were divided into 6 groups ( n = 6) in the pharmacodynamic study to analyze the mechanism of DAP activity: the control, sham, CCI, positive (3 mg/mL morphine) (850807, Qinghai pharmaceutical factory, Qinghai, China), DAP high-dose (DAPH; 0.5 mg/mL) (B21084, Yuanye Biotechnology Co., Ltd., Shanghai, China), and DAP low-dose (DAPL; 0.2 mg/mL) groups.

Techniques: Activation Assay, Expressing, Immunofluorescence, Double Staining

Journal: Pharmaceuticals

Article Title: Daphnetin Ameliorates Neuropathic Pain via Regulation of Microglial Responses and Glycerophospholipid Metabolism in the Spinal Cord

doi: 10.3390/ph17060789

Figure Lengend Snippet: Analysis of DEP expression in the spinal cord samples among the sham, CCI, and DAP groups. ( A , B ) The volcano plot and clustering heat map of the DEPs between the CCI and sham groups. ( C , D ) The volcano plot and clustering heat map of the DEPs between the DAP and CCI groups.

Article Snippet: In experiment 1, rats were divided into 6 groups ( n = 6) in the pharmacodynamic study to analyze the mechanism of DAP activity: the control, sham, CCI, positive (3 mg/mL morphine) (850807, Qinghai pharmaceutical factory, Qinghai, China), DAP high-dose (DAPH; 0.5 mg/mL) (B21084, Yuanye Biotechnology Co., Ltd., Shanghai, China), and DAP low-dose (DAPL; 0.2 mg/mL) groups.

Techniques: Expressing

Journal: Pharmaceuticals

Article Title: Daphnetin Ameliorates Neuropathic Pain via Regulation of Microglial Responses and Glycerophospholipid Metabolism in the Spinal Cord

doi: 10.3390/ph17060789

Figure Lengend Snippet: GO and KEGG pathway enrichment analysis of the DEPs between the DAP and CCI groups. ( A , B ) GO enrichment analysis of the DEPs between the DAP and CCI groups. ( C , D ) KEGG pathway enrichment analysis of the DEPs between the DAP and CCI groups.

Article Snippet: In experiment 1, rats were divided into 6 groups ( n = 6) in the pharmacodynamic study to analyze the mechanism of DAP activity: the control, sham, CCI, positive (3 mg/mL morphine) (850807, Qinghai pharmaceutical factory, Qinghai, China), DAP high-dose (DAPH; 0.5 mg/mL) (B21084, Yuanye Biotechnology Co., Ltd., Shanghai, China), and DAP low-dose (DAPL; 0.2 mg/mL) groups.

Techniques:

Journal: Pharmaceuticals

Article Title: Daphnetin Ameliorates Neuropathic Pain via Regulation of Microglial Responses and Glycerophospholipid Metabolism in the Spinal Cord

doi: 10.3390/ph17060789

Figure Lengend Snippet: OPLS-DA score plots and permutation tests of spinal cord samples in the sham, CCI, and DAP groups. ( A , C ) OPLS-DA score plots and permutation tests in spinal cord samples between the sham and CCI groups. ( B , D ) OPLS-DA score plots and permutation tests between spinal cord samples from the DAP and CCI groups.

Article Snippet: In experiment 1, rats were divided into 6 groups ( n = 6) in the pharmacodynamic study to analyze the mechanism of DAP activity: the control, sham, CCI, positive (3 mg/mL morphine) (850807, Qinghai pharmaceutical factory, Qinghai, China), DAP high-dose (DAPH; 0.5 mg/mL) (B21084, Yuanye Biotechnology Co., Ltd., Shanghai, China), and DAP low-dose (DAPL; 0.2 mg/mL) groups.

Techniques:

Journal: Pharmaceuticals

Article Title: Daphnetin Ameliorates Neuropathic Pain via Regulation of Microglial Responses and Glycerophospholipid Metabolism in the Spinal Cord

doi: 10.3390/ph17060789

Figure Lengend Snippet: Expression analysis of DEMs in the spinal cord samples among the sham, CCI, and DAP groups. ( A ) Volcano plot of the DEPs between the CCI and sham groups. ( B ) Volcano plot of the DEPs between the CCI and DAP groups. ( C ) Venn diagram of DEMs in the CCI vs. sham and DAP vs. CCI groups. ( D ) Heat map of 36 DEPs between the CCI vs. sham and DAP vs. CCI groups.

Article Snippet: In experiment 1, rats were divided into 6 groups ( n = 6) in the pharmacodynamic study to analyze the mechanism of DAP activity: the control, sham, CCI, positive (3 mg/mL morphine) (850807, Qinghai pharmaceutical factory, Qinghai, China), DAP high-dose (DAPH; 0.5 mg/mL) (B21084, Yuanye Biotechnology Co., Ltd., Shanghai, China), and DAP low-dose (DAPL; 0.2 mg/mL) groups.

Techniques: Expressing

Journal: Pharmaceuticals

Article Title: Daphnetin Ameliorates Neuropathic Pain via Regulation of Microglial Responses and Glycerophospholipid Metabolism in the Spinal Cord

doi: 10.3390/ph17060789

Figure Lengend Snippet: KEGG enrichment analysis of DEMs between the DAP and CCI groups. ( A ) Circos map of pathways. ( B ) Bar map of pathways. ( C ) Bubble map of pathways. ( D ) Chord map of pathways.

Article Snippet: In experiment 1, rats were divided into 6 groups ( n = 6) in the pharmacodynamic study to analyze the mechanism of DAP activity: the control, sham, CCI, positive (3 mg/mL morphine) (850807, Qinghai pharmaceutical factory, Qinghai, China), DAP high-dose (DAPH; 0.5 mg/mL) (B21084, Yuanye Biotechnology Co., Ltd., Shanghai, China), and DAP low-dose (DAPL; 0.2 mg/mL) groups.

Techniques:

Journal: Pharmaceuticals

Article Title: Daphnetin Ameliorates Neuropathic Pain via Regulation of Microglial Responses and Glycerophospholipid Metabolism in the Spinal Cord

doi: 10.3390/ph17060789

Figure Lengend Snippet: Association analysis of DEPs and DEMs between the DAP and CCI groups. ( A ) KEGG pathways. ( B ) Heat map of the 42 DEPs between the DAP and CCI groups. ( C ) Glycerophospholipid metabolism pathway.

Article Snippet: In experiment 1, rats were divided into 6 groups ( n = 6) in the pharmacodynamic study to analyze the mechanism of DAP activity: the control, sham, CCI, positive (3 mg/mL morphine) (850807, Qinghai pharmaceutical factory, Qinghai, China), DAP high-dose (DAPH; 0.5 mg/mL) (B21084, Yuanye Biotechnology Co., Ltd., Shanghai, China), and DAP low-dose (DAPL; 0.2 mg/mL) groups.

Techniques:

Journal: Scientific Reports

Article Title: Boreal marine fauna from the Barents Sea disperse to Arctic Northeast Greenland

doi: 10.1038/s41598-019-42097-x

Figure Lengend Snippet: Genetic evidence of Atlantic cod ( Gadus morhua) ( a – c ), beaked redfish ( Sebastes mentella) ( d – f ) and deep-sea shrimp ( Pandalus borealis) ( g – i ) specimens found off Northeast Greenland originating from the Barents Sea. Maps ( a , d , g ) show species known distribution extent (shaded colours) in the Northeast Atlantic, catch sites of individuals in Northeast Greenland (NEG) waters (full circles), reference samples (hollow circles) and a proposed dispersal route (arrow). DAPC scatterplots ( b , e , h ) show how the NEG groups relate to the reference populations of the Northeast Atlantic Ocean. DAPC cluster ellipses were set to contain 95% of genotypes. DAPC scatterplots explain 94% ( b ), 92% ( e ) and 97% ( h ) of the total variation observed. STRUCTURE barplots ( c , f , i ) show membership probabilities ( q ) for NEG individuals based on the reference populations used. Maps were created using ESRI ArcMap (v. 10.6, www.arcgis.com ).

Article Snippet: Discriminant Analysis of Principle Components (DAPC) strongly support the assignment results by clustering the NE Greenland specimens with the corresponding Barents Sea populations (Fig. ).

Techniques:

Journal: Communications Biology

Article Title: NUPR1 protects against hyperPARylation-dependent cell death

doi: 10.1038/s42003-022-03705-1

Figure Lengend Snippet: a OXPHOS metabolism, reflected by oxygen consumption rate (OCR) levels were measured in MiaPaCa-2 cells after 24 h treatments. b MiaPaCa-2 cells were treated with ZZW-115 (1.5 µM) and olaparib (25 µM) for 24 h, then, loaded with MitoTracker Deep-Red FM and, after fixation, stained with DAPI. Flow cytometry analysis were carried out using MitoProbe™ TMRM ( c ), MitoSOX™ Red ( d ), CellROX™ Orange Reagent ( e ) or Fluo-4-AM ( f ) for analysis of the mitochondrial membrane potential, mitochondrial superoxide levels, intracellular ROS levels and the cytosolic calcium concentration, respectively, after 24 h of incubation with the drugs. Data represent mean ± SEM. Two-way, Sidak was used, * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001. g PLA was performed in MiaPaCa-2 cells in the presence or in the absence of ZZW-115 together or not with 5-FU in the presence or absence of olaparib. Mouse anti-PAR and rabbit anti-Mitofusin2 antibodies were used. A representative experiment is shown ( n = 3). ImageJ was used to count the number of green dots. Mean ± SEM of foci/nucleus are included.

Article Snippet: Finally, samples were mounted using the Prolong Gold antifade reagent with DAPI (Thermon Fisher).

Techniques: Staining, Flow Cytometry, Membrane, Concentration Assay, Incubation