Journal: Journal of integrative neuroscience

Article Title: Ginsenoside Rb1 attenuates lipopolysaccharide-induced neural damage in the brain of mice via regulating the dysfunction of microglia and astrocytes.

doi: 10.31083/j.jin2004084

Figure Lengend Snippet: Fig. 5. Rb1 treatment suppressed the LPS-induced microglial activation and inhibited the production of IL-1β in the brain. IBa-1 immunostaining and the IL-1β test were performed 24 h after LPS i.p. injection. The IBa-1 positive cells (Red) were shown in CA1, S1Tr, S1BF, and S2 area (A). The nuclei were stained with DAPI (Blue). The IBA1 cells in CA1, S1Tr, S1BF, and S2 were counted (B). The IL-1β production in the brain was analyzed with ELISA (C). Data are represented as mean ± SD (n = 6 or 9). ###p < 0.001 versus the control group; **p < 0.01 and ***p < 0.001 versus the LPS group.

Article Snippet: Tissues were weighed and homogenized in ice-cold normal saline, and amounts of IL-1β in the mouse brain were determined with ELISA Kits (Raybiotech, Norcross, GA,USA) according to themanufacturer’s instructions.

Techniques: Activation Assay, Immunostaining, Injection, Staining, Enzyme-linked Immunosorbent Assay, Control