Journal: International Journal of Molecular Sciences

Article Title: Human Wharton’s Jelly-Derived Mesenchymal Stem Cells Minimally Improve the Growth Kinetics and Cardiomyocyte Differentiation of Aged Murine Cardiac c-kit Cells in In Vitro without Rejuvenating Effect

doi: 10.3390/ijms20225519

Figure Lengend Snippet: List of antibodies for CC characterisation and differentiation.

Article Snippet: FITC Rat Anti-mouse Sca-1 Antibody (Clone D7) , 1:10 , FC , Miltenyi Biotec, Germany (130-102-297).

Techniques:

Journal: eLife

Article Title: Epigenetic regulation of Wnt7b expression by the cis -acting long noncoding RNA Lnc-Rewind in muscle stem cells

doi: 10.7554/eLife.54782

Figure Lengend Snippet: ( A ) Table represents the values obtained by analysing the local sequence alignment between the human and murine Lnc-Rewind transcripts. Data were produced by using the implementation of the Smith–Waterman algorithm available at http://www.ebi.ac.uk/Tools/psa/emboss_water/ . ( B ) Semiquantitative RT-PCR (sqRT-PCR) quantification of the human hs_Lnc-Rewind transcript in proliferating (GM) myoblasts from a healthy donor. Mature Gapdh was used as endogenous control. ( C ) FACS plot showing MuSC isolation strategy from WT mice. MuSCs are isolated, among the lineage (CD45/CD31/Ter119) negative cells, as α7integrin+/Sca1− cells (magenta box) (left and middle panels). The right plot shows the check purity of sorted MuSCs. ( D ) qRT-PCR quantification of Myog and Mck in C 2 C 12 and MuSC-derived myoblasts in growth (GM) and differentiated (DM) conditions. Data represent the mean ± SEM of three biological replicates and were normalized on Gapdh mRNA. ( E ) sqRT-PCR quantification of Lnc-Rewind, using different primers, in cytoplasmic (Cyt) and nuclear (Nuc) fractions from proliferating C 2 C 12 and MuSC-derived myoblasts. The quality of fractionation was tested with mature ( Gapdh ) and precursor ( pre-Gapdh ) RNAs. –rt represents the negative control ( F ) Representative 60X confocal images of MuSC-derived myoblasts cell cultures hybridized with probes set specific for Lnc-Rewind (upper panels) and for a human mRNA (dlc1), as negative control (neg ctrl) (bottom panels). Autofluorescence (gray) is shown with false colour to visualize the cell body. DAPI, 4’,6-diamidino-2-phenylindole (blue); scale bar: 25 μm. ( G ) Representative 60× confocal images of C 2 C 12 cell cultures hybridized with probes set specific for Lnc-Rewind (upper panels) and for a human mRNA (dlc1), as negative control (neg ctrl) (bottom panels). Autofluorescence (grey) is shown with false colour to visualize the cell body. DAPI, 4’,6-diamidino-2-phenylindole (blue); scale bar: 25 μm. Data information: *p<0.05, unpaired Student’s t-test. Figure 1—figure supplement 1—source data 1. Source data for .

Article Snippet: Cells were incubated with primary antibodies CD31-PE (MiltenyBiotec, 130111540; RRID: AB_2657296 ), CD45-PE (MiltenyBiotec, 139110797; RRID: AB_2658218 ), Ter119-PE (MiltenyBiotec, 130112909; RRID: AB_2654115 ) 1:25; Sca1-FITC (MiltenyBiotec, 130116490; RRID: AB_2751322 ) 1:50; α7Integrin-APCVio770 (MiltenyBiotec, 130095212; Custom) 1:20 for 45 min on ice diluted in HBSS containing 0.2% BSA, 1% penicillin-streptomycin, and 1% DNAse I.

Techniques: Sequencing, Produced, Reverse Transcription Polymerase Chain Reaction, Control, Isolation, Quantitative RT-PCR, Derivative Assay, Fractionation, Negative Control

Journal: eLife

Article Title: Epigenetic regulation of Wnt7b expression by the cis -acting long noncoding RNA Lnc-Rewind in muscle stem cells

doi: 10.7554/eLife.54782

Figure Lengend Snippet:

Article Snippet: Cells were incubated with primary antibodies CD31-PE (MiltenyBiotec, 130111540; RRID: AB_2657296 ), CD45-PE (MiltenyBiotec, 139110797; RRID: AB_2658218 ), Ter119-PE (MiltenyBiotec, 130112909; RRID: AB_2654115 ) 1:25; Sca1-FITC (MiltenyBiotec, 130116490; RRID: AB_2751322 ) 1:50; α7Integrin-APCVio770 (MiltenyBiotec, 130095212; Custom) 1:20 for 45 min on ice diluted in HBSS containing 0.2% BSA, 1% penicillin-streptomycin, and 1% DNAse I.

Techniques: Sequencing, Control, Clone Assay, SYBR Green Assay, Magnetic Beads, Recombinant, Software

Journal: Respiratory Research

Article Title: Integrin α8 is a useful cell surface marker of alveolar lipofibroblasts

doi: 10.1186/s12931-025-03103-1

Figure Lengend Snippet: Antibodies and staining materials used in the present study

Article Snippet: Ly6a (Sca-1) , Miltenyi Biotec , 130-123-848 , IHC , 1:100.

Techniques: Staining

Journal: Respiratory Research

Article Title: Integrin α8 is a useful cell surface marker of alveolar lipofibroblasts

doi: 10.1186/s12931-025-03103-1

Figure Lengend Snippet: Localization and transcriptome profiles of ITGA8 + or SCA-1 + lung fibroblasts. ( A ) Representative images of a Pdgfra-GFP mouse lung stained with anti-ITGA8 antibody ( upper )/anti-SCA-1 antibody ( lower ), anti-SFTPC antibody ( upper ), and DAPI ( scale bars: 20 μm ). Triangles show ITGA8 + GFP + fibroblasts. Arrows show SCA-1 + GFP + fibroblasts. Br = bronchus; Ar = artery. The results are obtained from three independent adult male mice. ( B ) Quantifications of the distances from the nearest SFTPC-positive alveolar epithelial cell type 2 (AT2) in ITGA8-positive, SCA-1-positive, or ITGA8-negative fibroblasts. One hundred fibroblasts in each fibroblast subpopulation of the mouse lung are counted and the data are obtained from three independent Pdgfra-GFP mice (#1, #2, and #3). *** P < 0.001 (one-way analysis of variance with Bonferroni correction). ( C ) Reanalysis of single cell RNA-seq data of mouse lung cells isolated from Col1a1-GFP mice (GSE132771). Clustering of subpopulations ( left upper ) and visualization of mRNA expressions of Pdgfra , Itga8 , and Ly6a . The lower panels show the dot plot analysis for selected genes between Ly6a + and Itga8 + fibroblasts. ( D ) Analysis of our original single cell RNA-seq data of mouse lung mesenchymal cells isolated from wild-type mice (GSE244237). Clustering of subpopulations ( left upper ) and visualization of mRNA expressions of Pdgfra , Itga8 , and Ly6a . The lower panels show the dot plot analysis for selected genes between Ly6a + and Itga8 + fibroblasts

Article Snippet: Ly6a (Sca-1) , Miltenyi Biotec , 130-123-848 , IHC , 1:100.

Techniques: Staining, RNA Sequencing, Isolation