luria broth Search Results


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  • 96
    Thermo Fisher luria broth
    Luria Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 477 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria broth/product/Thermo Fisher
    Average 96 stars, based on 477 article reviews
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    96
    Millipore luria broth
    The predicted Flp/Tad pilus has no effect on growth, PCWDEs, motility or biofilm formation in vitro . A) In vitro growth of P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) in <t>hrp</t> -inducing minimal media supplemented with 10% v/v potato tuber extract. P. wasabiae and P. atrosepticum reached different cell densities, although there was no significant difference between the wild-type strains and the corresponding mutant strains. B) Production of plant cell wall-degrading enzymes (PCWDEs) in P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) growing on indicator plates containing 0.7% polygalacturonic acid (PGA) or 0.5% carboxymethylcellulose (CMC). In the figure, “average Ø” indicates the diameter of the halo around the bacteria in centimeters. The averages and standard deviations (SD) of four replicates (n = 4) are provided, and the experiment was repeated a minimum of three times with similar results. C) Flagella-based motility of P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) on 0.25% agar plates. All strains were motile, and no significant differences in spreading were observed. D) The in vitro biofilm formation ability of P. atrosepticum SCRI1043 and P. wasabiae SCC3193 differed in 0.4% glycerol after 18 h of incubation, but there was no significant difference between the wild-type strains and the corresponding mutant strains (Δ flp / tad , ΔECA0785, Δ flp ). E) The in vitro biofilm formation of P. atrosepticum SCRI1043 and P. wasabiae SCC3193 differed in <t>Luria</t> broth after 6 h of incubation, but there was no difference between the wild-type strains and the corresponding mutant strains (Δ flp / tad , ΔECA0785 and Δ flp ). All experiments were repeated a minimum of three times with a minimum of three replicates. The figures represent the averages and standard deviations of one experiment.
    Luria Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 868 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Difco luria broth
    The predicted Flp/Tad pilus has no effect on growth, PCWDEs, motility or biofilm formation in vitro . A) In vitro growth of P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) in <t>hrp</t> -inducing minimal media supplemented with 10% v/v potato tuber extract. P. wasabiae and P. atrosepticum reached different cell densities, although there was no significant difference between the wild-type strains and the corresponding mutant strains. B) Production of plant cell wall-degrading enzymes (PCWDEs) in P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) growing on indicator plates containing 0.7% polygalacturonic acid (PGA) or 0.5% carboxymethylcellulose (CMC). In the figure, “average Ø” indicates the diameter of the halo around the bacteria in centimeters. The averages and standard deviations (SD) of four replicates (n = 4) are provided, and the experiment was repeated a minimum of three times with similar results. C) Flagella-based motility of P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) on 0.25% agar plates. All strains were motile, and no significant differences in spreading were observed. D) The in vitro biofilm formation ability of P. atrosepticum SCRI1043 and P. wasabiae SCC3193 differed in 0.4% glycerol after 18 h of incubation, but there was no significant difference between the wild-type strains and the corresponding mutant strains (Δ flp / tad , ΔECA0785, Δ flp ). E) The in vitro biofilm formation of P. atrosepticum SCRI1043 and P. wasabiae SCC3193 differed in <t>Luria</t> broth after 6 h of incubation, but there was no difference between the wild-type strains and the corresponding mutant strains (Δ flp / tad , ΔECA0785 and Δ flp ). All experiments were repeated a minimum of three times with a minimum of three replicates. The figures represent the averages and standard deviations of one experiment.
    Luria Broth, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 572 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria broth/product/Difco
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    92
    Fisher Scientific luria broth
    The predicted Flp/Tad pilus has no effect on growth, PCWDEs, motility or biofilm formation in vitro . A) In vitro growth of P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) in <t>hrp</t> -inducing minimal media supplemented with 10% v/v potato tuber extract. P. wasabiae and P. atrosepticum reached different cell densities, although there was no significant difference between the wild-type strains and the corresponding mutant strains. B) Production of plant cell wall-degrading enzymes (PCWDEs) in P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) growing on indicator plates containing 0.7% polygalacturonic acid (PGA) or 0.5% carboxymethylcellulose (CMC). In the figure, “average Ø” indicates the diameter of the halo around the bacteria in centimeters. The averages and standard deviations (SD) of four replicates (n = 4) are provided, and the experiment was repeated a minimum of three times with similar results. C) Flagella-based motility of P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) on 0.25% agar plates. All strains were motile, and no significant differences in spreading were observed. D) The in vitro biofilm formation ability of P. atrosepticum SCRI1043 and P. wasabiae SCC3193 differed in 0.4% glycerol after 18 h of incubation, but there was no significant difference between the wild-type strains and the corresponding mutant strains (Δ flp / tad , ΔECA0785, Δ flp ). E) The in vitro biofilm formation of P. atrosepticum SCRI1043 and P. wasabiae SCC3193 differed in <t>Luria</t> broth after 6 h of incubation, but there was no difference between the wild-type strains and the corresponding mutant strains (Δ flp / tad , ΔECA0785 and Δ flp ). All experiments were repeated a minimum of three times with a minimum of three replicates. The figures represent the averages and standard deviations of one experiment.
    Luria Broth, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 74 article reviews
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    92
    HiMedia Laboratories luria broth
    Expression analysis of phrD in P . aeruginosa strain PAO1. ( a ) Northern blots of PhrD in over expression and disruption. Lane 1-pHERD phrD : over expression; lane 2-pHERD30T: vector control; lane 3- phrD ΩGm: disruption strain. Ethidium bromide stained 23S and 16S rRNA bands on agarose gels are shown as loading controls. ( b ) Time course qRT-PCR of phrD from the WT in LB, normalized with 16S rRNA and 0 h expression as the calibrator. ( c ) phrD . ( d ) Comparative PhrD levels in WT under nitrogen limited <t>MMP</t> and phosphate limited PPGAS media compared to that in <t>Luria</t> broth.
    Luria Broth, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Laboratorios Conda luria broth
    Expression analysis of phrD in P . aeruginosa strain PAO1. ( a ) Northern blots of PhrD in over expression and disruption. Lane 1-pHERD phrD : over expression; lane 2-pHERD30T: vector control; lane 3- phrD ΩGm: disruption strain. Ethidium bromide stained 23S and 16S rRNA bands on agarose gels are shown as loading controls. ( b ) Time course qRT-PCR of phrD from the WT in LB, normalized with 16S rRNA and 0 h expression as the calibrator. ( c ) phrD . ( d ) Comparative PhrD levels in WT under nitrogen limited <t>MMP</t> and phosphate limited PPGAS media compared to that in <t>Luria</t> broth.
    Luria Broth, supplied by Laboratorios Conda, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Research Products International luria broth
    Expression analysis of phrD in P . aeruginosa strain PAO1. ( a ) Northern blots of PhrD in over expression and disruption. Lane 1-pHERD phrD : over expression; lane 2-pHERD30T: vector control; lane 3- phrD ΩGm: disruption strain. Ethidium bromide stained 23S and 16S rRNA bands on agarose gels are shown as loading controls. ( b ) Time course qRT-PCR of phrD from the WT in LB, normalized with 16S rRNA and 0 h expression as the calibrator. ( c ) phrD . ( d ) Comparative PhrD levels in WT under nitrogen limited <t>MMP</t> and phosphate limited PPGAS media compared to that in <t>Luria</t> broth.
    Luria Broth, supplied by Research Products International, used in various techniques. Bioz Stars score: 92/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson luria broth
    Expression analysis of phrD in P . aeruginosa strain PAO1. ( a ) Northern blots of PhrD in over expression and disruption. Lane 1-pHERD phrD : over expression; lane 2-pHERD30T: vector control; lane 3- phrD ΩGm: disruption strain. Ethidium bromide stained 23S and 16S rRNA bands on agarose gels are shown as loading controls. ( b ) Time course qRT-PCR of phrD from the WT in LB, normalized with 16S rRNA and 0 h expression as the calibrator. ( c ) phrD . ( d ) Comparative PhrD levels in WT under nitrogen limited <t>MMP</t> and phosphate limited PPGAS media compared to that in <t>Luria</t> broth.
    Luria Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 307 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Carl Roth GmbH luria broth
    Expression analysis of phrD in P . aeruginosa strain PAO1. ( a ) Northern blots of PhrD in over expression and disruption. Lane 1-pHERD phrD : over expression; lane 2-pHERD30T: vector control; lane 3- phrD ΩGm: disruption strain. Ethidium bromide stained 23S and 16S rRNA bands on agarose gels are shown as loading controls. ( b ) Time course qRT-PCR of phrD from the WT in LB, normalized with 16S rRNA and 0 h expression as the calibrator. ( c ) phrD . ( d ) Comparative PhrD levels in WT under nitrogen limited <t>MMP</t> and phosphate limited PPGAS media compared to that in <t>Luria</t> broth.
    Luria Broth, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Amresco luria broth
    Expression analysis of phrD in P . aeruginosa strain PAO1. ( a ) Northern blots of PhrD in over expression and disruption. Lane 1-pHERD phrD : over expression; lane 2-pHERD30T: vector control; lane 3- phrD ΩGm: disruption strain. Ethidium bromide stained 23S and 16S rRNA bands on agarose gels are shown as loading controls. ( b ) Time course qRT-PCR of phrD from the WT in LB, normalized with 16S rRNA and 0 h expression as the calibrator. ( c ) phrD . ( d ) Comparative PhrD levels in WT under nitrogen limited <t>MMP</t> and phosphate limited PPGAS media compared to that in <t>Luria</t> broth.
    Luria Broth, supplied by Amresco, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    luria broth - by Bioz Stars, 2020-08
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    92
    Melford Laboratories luria broth
    Expression analysis of phrD in P . aeruginosa strain PAO1. ( a ) Northern blots of PhrD in over expression and disruption. Lane 1-pHERD phrD : over expression; lane 2-pHERD30T: vector control; lane 3- phrD ΩGm: disruption strain. Ethidium bromide stained 23S and 16S rRNA bands on agarose gels are shown as loading controls. ( b ) Time course qRT-PCR of phrD from the WT in LB, normalized with 16S rRNA and 0 h expression as the calibrator. ( c ) phrD . ( d ) Comparative PhrD levels in WT under nitrogen limited <t>MMP</t> and phosphate limited PPGAS media compared to that in <t>Luria</t> broth.
    Luria Broth, supplied by Melford Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Teknova luria broth
    Expression analysis of phrD in P . aeruginosa strain PAO1. ( a ) Northern blots of PhrD in over expression and disruption. Lane 1-pHERD phrD : over expression; lane 2-pHERD30T: vector control; lane 3- phrD ΩGm: disruption strain. Ethidium bromide stained 23S and 16S rRNA bands on agarose gels are shown as loading controls. ( b ) Time course qRT-PCR of phrD from the WT in LB, normalized with 16S rRNA and 0 h expression as the calibrator. ( c ) phrD . ( d ) Comparative PhrD levels in WT under nitrogen limited <t>MMP</t> and phosphate limited PPGAS media compared to that in <t>Luria</t> broth.
    Luria Broth, supplied by Teknova, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    BioShop luria broth
    Expression analysis of phrD in P . aeruginosa strain PAO1. ( a ) Northern blots of PhrD in over expression and disruption. Lane 1-pHERD phrD : over expression; lane 2-pHERD30T: vector control; lane 3- phrD ΩGm: disruption strain. Ethidium bromide stained 23S and 16S rRNA bands on agarose gels are shown as loading controls. ( b ) Time course qRT-PCR of phrD from the WT in LB, normalized with 16S rRNA and 0 h expression as the calibrator. ( c ) phrD . ( d ) Comparative PhrD levels in WT under nitrogen limited <t>MMP</t> and phosphate limited PPGAS media compared to that in <t>Luria</t> broth.
    Luria Broth, supplied by BioShop, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    scharlau luria broth
    Expression analysis of phrD in P . aeruginosa strain PAO1. ( a ) Northern blots of PhrD in over expression and disruption. Lane 1-pHERD phrD : over expression; lane 2-pHERD30T: vector control; lane 3- phrD ΩGm: disruption strain. Ethidium bromide stained 23S and 16S rRNA bands on agarose gels are shown as loading controls. ( b ) Time course qRT-PCR of phrD from the WT in LB, normalized with 16S rRNA and 0 h expression as the calibrator. ( c ) phrD . ( d ) Comparative PhrD levels in WT under nitrogen limited <t>MMP</t> and phosphate limited PPGAS media compared to that in <t>Luria</t> broth.
    Luria Broth, supplied by scharlau, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    US Biological Life Sciences luria broth
    Expression analysis of phrD in P . aeruginosa strain PAO1. ( a ) Northern blots of PhrD in over expression and disruption. Lane 1-pHERD phrD : over expression; lane 2-pHERD30T: vector control; lane 3- phrD ΩGm: disruption strain. Ethidium bromide stained 23S and 16S rRNA bands on agarose gels are shown as loading controls. ( b ) Time course qRT-PCR of phrD from the WT in LB, normalized with 16S rRNA and 0 h expression as the calibrator. ( c ) phrD . ( d ) Comparative PhrD levels in WT under nitrogen limited <t>MMP</t> and phosphate limited PPGAS media compared to that in <t>Luria</t> broth.
    Luria Broth, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore luria bertani broth
    Growth of DH5α cells containing pET28a, pGreenII, and pMET1-03. (A) Cell morphology. Aliquots of culture were viewed using a light microscope under phase contrast at 100 × magnification with an oil immersion objective. A graticule was used to provide a measuring scale. The length of the sides of the visible squares was 50 µm. The length of a typical E. coli cell is 2 µm. (B) The growth of E. coli using overnight cultures as an inoculum. 50 ml of LB media (containing 50 µg/ml kanamycin) within a 250 ml Erlenmeyer flask was inoculated with 0.2 OD 600 units of cells from a 5 ml overnight culture (see Materials and Methods ). Growth was monitored by measuring OD 600 values. The data-points for pET28a and pGreenII are represented by diamonds and squares, respectively. (C) Colony morphology. The pictures are of primary transformants following overnight incubation. The graduations at the bottom of each image in this panel correspond to 1 mm. (D) Restriction enzyme analysis of a selection of plasmids isolated from mutants that produce large colonies. The lane labeled M contains the 1 kb Plus DNA Ladder (Life Technologies). The mutants are in lanes 1–16. Numbering on the left of the panel indicates the expected sizes of the two fragments produced by Eco RI digestion of pMET1-03. The smaller of the two fragments corresponds to the MET1 cassette destined for plants. Labels that are outlined indicate plasmids with obvious rearrangements. The gel used for electrophoresis was composed of 0.8% [w/v] agarose and stained with ethidium bromide. LB, <t>Luria</t> <t>Bertani;</t> OD, optical density.
    Luria Bertani Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Difco luria bertani broth
    Biofilm formation by A . pleuropneumoniae serotype 1 reference strain S4074 grown in different culture media using the crystal violet staining protocol described in Materials and methods. LB: <t>Luria-Bertani;</t> TSB: tryptic soy broth; M-H: Mueller Hinton; BHI: brain heart infusion.
    Luria Bertani Broth, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 976 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The predicted Flp/Tad pilus has no effect on growth, PCWDEs, motility or biofilm formation in vitro . A) In vitro growth of P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) in hrp -inducing minimal media supplemented with 10% v/v potato tuber extract. P. wasabiae and P. atrosepticum reached different cell densities, although there was no significant difference between the wild-type strains and the corresponding mutant strains. B) Production of plant cell wall-degrading enzymes (PCWDEs) in P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) growing on indicator plates containing 0.7% polygalacturonic acid (PGA) or 0.5% carboxymethylcellulose (CMC). In the figure, “average Ø” indicates the diameter of the halo around the bacteria in centimeters. The averages and standard deviations (SD) of four replicates (n = 4) are provided, and the experiment was repeated a minimum of three times with similar results. C) Flagella-based motility of P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) on 0.25% agar plates. All strains were motile, and no significant differences in spreading were observed. D) The in vitro biofilm formation ability of P. atrosepticum SCRI1043 and P. wasabiae SCC3193 differed in 0.4% glycerol after 18 h of incubation, but there was no significant difference between the wild-type strains and the corresponding mutant strains (Δ flp / tad , ΔECA0785, Δ flp ). E) The in vitro biofilm formation of P. atrosepticum SCRI1043 and P. wasabiae SCC3193 differed in Luria broth after 6 h of incubation, but there was no difference between the wild-type strains and the corresponding mutant strains (Δ flp / tad , ΔECA0785 and Δ flp ). All experiments were repeated a minimum of three times with a minimum of three replicates. The figures represent the averages and standard deviations of one experiment.

    Journal: PLoS ONE

    Article Title: Role and Regulation of the Flp/Tad Pilus in the Virulence of Pectobacterium atrosepticum SCRI1043 and Pectobacterium wasabiae SCC3193

    doi: 10.1371/journal.pone.0073718

    Figure Lengend Snippet: The predicted Flp/Tad pilus has no effect on growth, PCWDEs, motility or biofilm formation in vitro . A) In vitro growth of P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) in hrp -inducing minimal media supplemented with 10% v/v potato tuber extract. P. wasabiae and P. atrosepticum reached different cell densities, although there was no significant difference between the wild-type strains and the corresponding mutant strains. B) Production of plant cell wall-degrading enzymes (PCWDEs) in P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) growing on indicator plates containing 0.7% polygalacturonic acid (PGA) or 0.5% carboxymethylcellulose (CMC). In the figure, “average Ø” indicates the diameter of the halo around the bacteria in centimeters. The averages and standard deviations (SD) of four replicates (n = 4) are provided, and the experiment was repeated a minimum of three times with similar results. C) Flagella-based motility of P. atrosepticum SCRI1043, P. wasabiae SCC3193 and their derivatives (Δ flp / tad , ΔECA0785 and Δ flp ) on 0.25% agar plates. All strains were motile, and no significant differences in spreading were observed. D) The in vitro biofilm formation ability of P. atrosepticum SCRI1043 and P. wasabiae SCC3193 differed in 0.4% glycerol after 18 h of incubation, but there was no significant difference between the wild-type strains and the corresponding mutant strains (Δ flp / tad , ΔECA0785, Δ flp ). E) The in vitro biofilm formation of P. atrosepticum SCRI1043 and P. wasabiae SCC3193 differed in Luria broth after 6 h of incubation, but there was no difference between the wild-type strains and the corresponding mutant strains (Δ flp / tad , ΔECA0785 and Δ flp ). All experiments were repeated a minimum of three times with a minimum of three replicates. The figures represent the averages and standard deviations of one experiment.

    Article Snippet: For gene expression studies by relative qPCR, bacteria were cultured until late log phase in Luria broth or hrp -inducing minimal medium salts supplemented with 0.4% polygalacturonic acid (PGA, P3850; Sigma-Aldrich) or 10% v/v potato tuber extract at 15°C (for microarray validation) or 28°C (for examining flp /tad -related genes).

    Techniques: In Vitro, Mutagenesis, Incubation

    Expression analysis of phrD in P . aeruginosa strain PAO1. ( a ) Northern blots of PhrD in over expression and disruption. Lane 1-pHERD phrD : over expression; lane 2-pHERD30T: vector control; lane 3- phrD ΩGm: disruption strain. Ethidium bromide stained 23S and 16S rRNA bands on agarose gels are shown as loading controls. ( b ) Time course qRT-PCR of phrD from the WT in LB, normalized with 16S rRNA and 0 h expression as the calibrator. ( c ) phrD . ( d ) Comparative PhrD levels in WT under nitrogen limited MMP and phosphate limited PPGAS media compared to that in Luria broth.

    Journal: Scientific Reports

    Article Title: Quorum sensing in Pseudomonas aeruginosa mediated by RhlR is regulated by a small RNA PhrD

    doi: 10.1038/s41598-018-36488-9

    Figure Lengend Snippet: Expression analysis of phrD in P . aeruginosa strain PAO1. ( a ) Northern blots of PhrD in over expression and disruption. Lane 1-pHERD phrD : over expression; lane 2-pHERD30T: vector control; lane 3- phrD ΩGm: disruption strain. Ethidium bromide stained 23S and 16S rRNA bands on agarose gels are shown as loading controls. ( b ) Time course qRT-PCR of phrD from the WT in LB, normalized with 16S rRNA and 0 h expression as the calibrator. ( c ) phrD . ( d ) Comparative PhrD levels in WT under nitrogen limited MMP and phosphate limited PPGAS media compared to that in Luria broth.

    Article Snippet: 200 µl cultures were periodically withdrawn from cells growing in Luria broth, MMP or PPGAS media. β -galactosidase activity was determined as described previously after normalization with protein in mg .

    Techniques: Expressing, Northern Blot, Over Expression, Plasmid Preparation, Staining, Quantitative RT-PCR

    PhrD positively regulates rhlR expression in P . aeruginosa PAO1. Time course measurement of RhlR levels by β- galactosidase activity of P3 rhlR::lacZ fusion (i), and qRT-PCR (ii), measured in WT and phrD disruption mutant under: ( a ) Phosphate limited PPGAS medium ( b ) Luria broth; ( c ) N-limited MMP medium. In case of MMP, the cells were first grown to the desired OD in N-rich medium and resuspended in N-limited MMP. There was no net growth owing to nitrogen starvation. β- galactosidase activity of the disruption strain complemented with phrD over expression plasmid is measured in Luria broth. The data is represented as the mean ± SD of three individual experiments and each sample was analyzed in triplicates.

    Journal: Scientific Reports

    Article Title: Quorum sensing in Pseudomonas aeruginosa mediated by RhlR is regulated by a small RNA PhrD

    doi: 10.1038/s41598-018-36488-9

    Figure Lengend Snippet: PhrD positively regulates rhlR expression in P . aeruginosa PAO1. Time course measurement of RhlR levels by β- galactosidase activity of P3 rhlR::lacZ fusion (i), and qRT-PCR (ii), measured in WT and phrD disruption mutant under: ( a ) Phosphate limited PPGAS medium ( b ) Luria broth; ( c ) N-limited MMP medium. In case of MMP, the cells were first grown to the desired OD in N-rich medium and resuspended in N-limited MMP. There was no net growth owing to nitrogen starvation. β- galactosidase activity of the disruption strain complemented with phrD over expression plasmid is measured in Luria broth. The data is represented as the mean ± SD of three individual experiments and each sample was analyzed in triplicates.

    Article Snippet: 200 µl cultures were periodically withdrawn from cells growing in Luria broth, MMP or PPGAS media. β -galactosidase activity was determined as described previously after normalization with protein in mg .

    Techniques: Expressing, Activity Assay, Quantitative RT-PCR, Mutagenesis, Over Expression, Plasmid Preparation

    Growth of DH5α cells containing pET28a, pGreenII, and pMET1-03. (A) Cell morphology. Aliquots of culture were viewed using a light microscope under phase contrast at 100 × magnification with an oil immersion objective. A graticule was used to provide a measuring scale. The length of the sides of the visible squares was 50 µm. The length of a typical E. coli cell is 2 µm. (B) The growth of E. coli using overnight cultures as an inoculum. 50 ml of LB media (containing 50 µg/ml kanamycin) within a 250 ml Erlenmeyer flask was inoculated with 0.2 OD 600 units of cells from a 5 ml overnight culture (see Materials and Methods ). Growth was monitored by measuring OD 600 values. The data-points for pET28a and pGreenII are represented by diamonds and squares, respectively. (C) Colony morphology. The pictures are of primary transformants following overnight incubation. The graduations at the bottom of each image in this panel correspond to 1 mm. (D) Restriction enzyme analysis of a selection of plasmids isolated from mutants that produce large colonies. The lane labeled M contains the 1 kb Plus DNA Ladder (Life Technologies). The mutants are in lanes 1–16. Numbering on the left of the panel indicates the expected sizes of the two fragments produced by Eco RI digestion of pMET1-03. The smaller of the two fragments corresponds to the MET1 cassette destined for plants. Labels that are outlined indicate plasmids with obvious rearrangements. The gel used for electrophoresis was composed of 0.8% [w/v] agarose and stained with ethidium bromide. LB, Luria Bertani; OD, optical density.

    Journal: G3: Genes|Genomes|Genetics

    Article Title: An Improved Binary Vector and Escherichia coli Strain for Agrobacterium tumefaciens-Mediated Plant Transformation

    doi: 10.1534/g3.116.029405

    Figure Lengend Snippet: Growth of DH5α cells containing pET28a, pGreenII, and pMET1-03. (A) Cell morphology. Aliquots of culture were viewed using a light microscope under phase contrast at 100 × magnification with an oil immersion objective. A graticule was used to provide a measuring scale. The length of the sides of the visible squares was 50 µm. The length of a typical E. coli cell is 2 µm. (B) The growth of E. coli using overnight cultures as an inoculum. 50 ml of LB media (containing 50 µg/ml kanamycin) within a 250 ml Erlenmeyer flask was inoculated with 0.2 OD 600 units of cells from a 5 ml overnight culture (see Materials and Methods ). Growth was monitored by measuring OD 600 values. The data-points for pET28a and pGreenII are represented by diamonds and squares, respectively. (C) Colony morphology. The pictures are of primary transformants following overnight incubation. The graduations at the bottom of each image in this panel correspond to 1 mm. (D) Restriction enzyme analysis of a selection of plasmids isolated from mutants that produce large colonies. The lane labeled M contains the 1 kb Plus DNA Ladder (Life Technologies). The mutants are in lanes 1–16. Numbering on the left of the panel indicates the expected sizes of the two fragments produced by Eco RI digestion of pMET1-03. The smaller of the two fragments corresponds to the MET1 cassette destined for plants. Labels that are outlined indicate plasmids with obvious rearrangements. The gel used for electrophoresis was composed of 0.8% [w/v] agarose and stained with ethidium bromide. LB, Luria Bertani; OD, optical density.

    Article Snippet: 0179) as described were grown in Luria Bertani broth (Sigma) with kanamycin selection (50 µg/ml) and shaking (200 rpm) at 37°.

    Techniques: Light Microscopy, Incubation, Selection, Isolation, Labeling, Produced, Electrophoresis, Staining

    Biofilm formation by A . pleuropneumoniae serotype 1 reference strain S4074 grown in different culture media using the crystal violet staining protocol described in Materials and methods. LB: Luria-Bertani; TSB: tryptic soy broth; M-H: Mueller Hinton; BHI: brain heart infusion.

    Journal: Veterinary Research

    Article Title: Effects of growth conditions on biofilm formation by Actinobacillus pleuropneumoniae

    doi: 10.1051/vetres/2009051

    Figure Lengend Snippet: Biofilm formation by A . pleuropneumoniae serotype 1 reference strain S4074 grown in different culture media using the crystal violet staining protocol described in Materials and methods. LB: Luria-Bertani; TSB: tryptic soy broth; M-H: Mueller Hinton; BHI: brain heart infusion.

    Article Snippet: A colony was transferred into 5 mL of Luria-Bertani broth (LB; Difco), tryptic soy broth (TSB; Difco), Mueller Hinton broth (MH; Difco) or BHI (BHI-A; Difco or BHI-B; Oxoid Ltd, Basingstoke, Hampshire, UK) with 5 μg/mL NAD and incubated at 37 °C overnight with agitation.

    Techniques: Staining