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Image Search Results
Journal: The Journal of Experimental Medicine
Article Title: Amelioration of epidermal hyperplasia by TNF inhibition is associated with reduced Th17 responses
doi: 10.1084/jem.20071094
Figure Lengend Snippet: Th17 cell products and downstream mediators are rapidly down-modulated with etanercept treatment compared with Th1 and Th2 cell products. mRNA expression normalized to HARP for (A) Th17 cell products IL-17 and IL-22 and (B) Th1 cell products IFN-γ and LTA-1. Error bars represent the mean ± SEM. (C) Multivariate U-statistics correlating the change in Th17 or Th1 cell products with histological response (epidermal thickness, K16, and Ki67) over time. (D) Downstream effectors of Th17 cells, CCL20, and DEFB4. (E) MX-1, downstream effector of Th1 cells. (F) Th2 cell product IL-4. All mRNA was evaluated in nonlesional skin (NL), lesional skin (LS), and in the lesional index plaque at weeks 1, 2, 4, and 12. Error bars represent the mean ± SEM. Baseline lesional values were compared with other time points. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
Article Snippet: Sequences of other primers and probes used in this study were as follows: CCL20 (MIP-3α) forward, GCTTTGATGTCAGTGCTGCTACTC; CCL20 reverse, GTATCCAAGACAGCAGTCAAAGTTG; CCL20 probe, FAM-TGCGGCGAATCAGAAGCAGCAA-TAMRA; IL-6 forward, CCAGGAGCCCAGCTATGAAC; IL-6 reverse, CCCAGGGAGAAGGCAACTG; IL-6 probe, FAM-CCTTCTCCACAAGCGCCTTCGGT-TAMRA; IL-4 forward, CGACTGCACAGCAGTTCCA; IL-4 reverse, AGGTTCCTGTCGAGCCGTTT; IL-4 probe, FAM-AGGCACAAGCAGCTGATCCGATTCC-TAMRA; IL-20 (Hs00218888_m1); IL-12 p35 (Hs00168405_m1); LTA-1 (
Techniques: Expressing
Journal: Schizophrenia research
Article Title: Abnormal gene and protein expression of inflammatory cytokines in the postmortem brain of schizophrenia patients
doi: 10.1016/j.schres.2017.04.043
Figure Lengend Snippet: TaqMan primers/probes used for qPCR analysis
Article Snippet: Relative expression levels are reported as fold change and ΔC t values are used for further statistical analysis (Applied Biosystems User Bulletin No. 2). table ft1 table-wrap mode="anchored" t5 caption a7 TaqMan accession Probe location (exon boundary) Assay function ACTB Hs99999903_m1 1–1 House Keeping (HK) GAPDH Hs99999905_m1 3–3 House Keeping (HK) TNF-α Hs99999043_m1 1–2 target gene IL-1β Hs01555410_m1 3–4 target gene IL-1RN (IL-1RA) Hs00893626_m1 4–5 target gene IL-2 Hs00174114_m1 2–3 target gene IL-6 Hs00985639_m1 2–3 target gene IL8 Hs00174103_m1 1–2 target gene IL10 Hs00961622_m1 4–5 target gene IL13 Hs00174379_m1 1–2 target gene LTA
Techniques: