Journal: mAbs

Article Title: Generation and in vivo characterization of a novel high-affinity human antibody targeting carcinoembryonic antigen

doi: 10.1080/19420862.2023.2217964

Figure Lengend Snippet: In vitro characterization of new anti-CEA antibodies. (a) SPR sensorgram of G9, F7, and F4 in a scFv format. K D values were calculated to be 640 nM, 50 nM, and 7.7 nM, respectively. (b) Flow cytometry analysis with the new antibodies in IgG format on CEA-expressing CT26 cells and on CEA-negative CT26 wild-type cells. (c) Immunofluorescence staining with IgG formats on the human colon adenocarcinoma xenograft LS174T. Anti-CEA antibodies were detected in green. Blood vessels were detected by CD31 staining (red). 20× magnification, scale bars = 100 μm.

Article Snippet: Immunofluorescence analysis was performed on LS174T and HT-29 xenografts harvested from mice, on patient-derived colon cancer samples, and on a human tissue microarray (Amsbio, T6235700–5).

Techniques: In Vitro, Flow Cytometry, Expressing, Immunofluorescence, Staining

Journal: mAbs

Article Title: Generation and in vivo characterization of a novel high-affinity human antibody targeting carcinoembryonic antigen

doi: 10.1080/19420862.2023.2217964

Figure Lengend Snippet: Ex vivo immunofluorescence-based biodistribution analysis. Immunofluorescence analysis assessed tumor targeting of new anti-CEA antibodies in IgG format. Two hundred micrograms of IgG-FITC were injected intravenously into LS174T-bearing mice. Tumors were excised 24 hours after injection. IgG-FITC was detected in green; blood vessels were detected through CD31 staining (red). 20× magnification, scale bars = 100 μm.

Article Snippet: Immunofluorescence analysis was performed on LS174T and HT-29 xenografts harvested from mice, on patient-derived colon cancer samples, and on a human tissue microarray (Amsbio, T6235700–5).

Techniques: Ex Vivo, Immunofluorescence, Injection, Staining

Journal: mAbs

Article Title: Generation and in vivo characterization of a novel high-affinity human antibody targeting carcinoembryonic antigen

doi: 10.1080/19420862.2023.2217964

Figure Lengend Snippet: Quantitative biodistribution with radiolabeled anti-CEA antibodies in diabody format. Quantitative biodistribution analysis of radio iodinated anti-CEA diabodies in BALB/c nude mice bearing subcutaneous LS174T colon adenocarcinomas. Organs were harvested 24 hours after intravenous injection, and radioactivity was quantified. Results are shown as the percentage of injected dose per gram (ID/g (%)). Error bars = SEM; n = 4.

Article Snippet: Immunofluorescence analysis was performed on LS174T and HT-29 xenografts harvested from mice, on patient-derived colon cancer samples, and on a human tissue microarray (Amsbio, T6235700–5).

Techniques: Injection, Radioactivity

Journal: ACS Omega

Article Title: Microbubble Enhanced Delivery of Vitamin C for Treatment of Colorectal Cancer

doi: 10.1021/acsomega.4c06779

Figure Lengend Snippet: LS174TIn VitroCell Response to PAL. LS174T cell viability following 1 h exposure to PAL and BL. The bar chart shows treatment with PAL compared to the carrier (BL), which used the same liposome preparation method and yielded a similar liposome concentration but omitted PA ( n = 3, error bars represent standard error for three biological repeats).

Article Snippet: LS174T human Caucasian colon adenocarcinoma (ECACC 87060401) and HCT116 human colon carcinoma (ECACC 91091005) cells were initially obtained from the European Collection of Authenticated Cell Cultures (ECACC, UK).

Techniques: Concentration Assay

Journal: ACS Omega

Article Title: Microbubble Enhanced Delivery of Vitamin C for Treatment of Colorectal Cancer

doi: 10.1021/acsomega.4c06779

Figure Lengend Snippet: Fluorescence maps and analysis of LS174T cells cultured on-chip and treated with PAL, MBs, and US. Treatment with (a) culture media only, or (b) PAL + MB + US (5×). Histograms showing the distribution of live and dead stained cells (dead pixel count multiplied by 10 to aid visualization), corresponding to the confocal images of LS174T cells post-treatment with (c) culture media or (d) PAL + MB + US (5×). Histograms showing the distribution of the fraction of dead LS174T cells (dead pixel count/total pixel count) which received treatment with (e) culture media only or (f) PAL + MB + US (5×).

Article Snippet: LS174T human Caucasian colon adenocarcinoma (ECACC 87060401) and HCT116 human colon carcinoma (ECACC 91091005) cells were initially obtained from the European Collection of Authenticated Cell Cultures (ECACC, UK).

Techniques: Fluorescence, Cell Culture, Staining

Journal: ACS Omega

Article Title: Microbubble Enhanced Delivery of Vitamin C for Treatment of Colorectal Cancer

doi: 10.1021/acsomega.4c06779

Figure Lengend Snippet: LS174T cell viability post BL, PAL, MB, and US exposures on-chip. (a) Cell viability data for LS174T cells on-chip, post-treatment with BL, PAL (5 mM), MB (1 × 10 8 MBs/mL), and US combinations ( n > 3 for all conditions, except for PAL (No US), conducted twice; error bars represent the standard error). Confocal images of stained LS174T cells following treatment with PAL + MB + US (5×) (b) inside and (c) outside of the US-exposed region. Confocal images of stained LS174T cells treated with culture media (DMEM) in the channel’s (d) central and (e) edge regions.

Article Snippet: LS174T human Caucasian colon adenocarcinoma (ECACC 87060401) and HCT116 human colon carcinoma (ECACC 91091005) cells were initially obtained from the European Collection of Authenticated Cell Cultures (ECACC, UK).

Techniques: Staining

Journal: PLoS ONE

Article Title: Genetic Ablation of Afadin Causes Mislocalization and Deformation of Paneth Cells in the Mouse Small Intestinal Epithelium

doi: 10.1371/journal.pone.0110549

Figure Lengend Snippet: ( A ) Western blotting of afadin and EphB3 using immunoprecipitates with the indicated antibodies from the Ls174T colon cancer cell line. ( B ) Immunostaining of the small intestine of control and afadin -cKO mice with antibodies against EphB3 (green), lysozyme (red), and Hoechst33258 (blue). Scale bars = 50 µm. ( C ) Relative intensity of EphB3 signal in crypt cells to that of non-crypt cells is shown. 18 crypts from 6 pictures (control) and 24 crypts from 8 pictures ( afadin -cKO) were analyzed.

Article Snippet: The colon cancer cell line Ls174T (DS Pharma Biomedical Co., Osaka, Japan) was cultured in MEM containing 1% NEAA, 2 mM L-glutamine, and 10% FBS and lysed in 50 mM Tris HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl 2 , 1% Nonidet P-40, 1 mM EGTA, and 10% glycerol supplemented with 1 μg/ml aprotinin, 1 μg/ml leupeptin, 20 μg/ml phenylmethylsulfonyl fluoride, and phosphatase inhibitors.

Techniques: Western Blot, Immunostaining, Control