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ImmunoTools
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New England Biolabs
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Journal: Cell Communication and Signaling : CCS
Article Title: NMDA-receptor antagonists block B-cell function but foster IL-10 production in BCR/CD40-activated B cells
doi: 10.1186/s12964-014-0075-5
Figure Lengend Snippet: NMDAR antagonists affect B-cell migration and Ig production. A) Ifenprodil impairs chemokine-induced B-cell migration. The migration of splenic B cells towards SDF-1α and CCL21 in the absence or presence of ifenprodil (30 μM) was determined and the relative + SD migration was calculated from three experiments. Migration of B cells in the absence of ifenprodil was set as 100%. B) Ifenprodil blocks Ig production. B cells were activated with LPS or LPS plus IL-4 in the presence or absence of ifenprodil (10 μM) and IgM and IgG secretion was determined at day 4 with ELISA. Ifenprodil was added at day 1, day 2 or day 3. Data are representative for two experiments.
Article Snippet: For detection of secreted IgM and IgG, B cells were activated with LPS (10 μg/ml) or
Techniques: Migration, Enzyme-linked Immunosorbent Assay