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  • 99
    Thermo Fisher o generuler ultra low range dna ladder
    Electrophoretic analysis of partial pep A gene (596 bp) of B. pseudomallei digested with Stu I and Hinc II restriction analysis. (12% polyacrylamide gel, 1X TBE buffer, 8 V/cm, 130 min); Lane M- <t>O’GeneRuler™</t> ultra low range <t>DNA</t> ladder; Lane 1- B. pseudomallei NCTC 13178; Lane 2- B. pseudomallei ATCC 23343; Lane 3- Type I; Lane 4- Type II; Lane 5- Type III.
    O Generuler Ultra Low Range Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/o generuler ultra low range dna ladder/product/Thermo Fisher
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    o generuler ultra low range dna ladder - by Bioz Stars, 2020-04
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    94
    Thermo Fisher low molecular weight rna marker
    In vivo expression and SL trans -splicing of a chimeric TnI/β-gal mRNA from a TnI/β-gal gene construct lacking the SL sequence. ( A ) Expression of β-gal, revealed by X-Gal staining (blue), in tail muscle of embryos 12 h following introduction of CiTnILacZ(−1.5) DNA into zygotes by electroporation. ( B ) RT–PCR amplification of β-gal mRNA with β-gal-specific leftward priming, and rightward priming with the SL primer. (Lane 1 ) Size markers (MBI <t>Fermentas</t> ladder mix, 10–0.1 kb; top visible band, 3 kb, bottom visible band 500 bp). (Lanes 2,3 ) RT–PCR products from two different batches of transfected embryos. The template in lane 4 was tRNA, used as a carrier in embryo <t>RNA</t> isolations. A 550-bp product, the size predicted for SL-ended β-gal mRNA, was produced from both embryo batches. [Production of this product required the presence of both primers and did not occur when CiTnILacZ(−1.5) plasmid DNA was used as the amplification template. An additional product of ∼400 bp seen in lanes 2 and 3 required only the β-gal-specific primer and was produced in control amplifications of CiTnILacZ(−1.5) plasmid DNA; it apparently results from rightward mis-priming by the β-gal-specific primer upstream of its normal leftward priming site.] ( C ) DNA sequence of 550-bp RT–PCR product. The 550-bp product (as in B ) was recovered and sequenced using the SL primer ( right ) and β-gal-specific primer ( left ). The leftward sequence confirmed the presence of the SL primer (bold) immediately upstream of TnI mRNA nucleotide 17. Not shown is the Bam HI site engineered at the 5′-end of the SL primer. Sequences deriving from the β-gal reporter gene are shown in lower case.
    Low Molecular Weight Rna Marker, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low molecular weight rna marker/product/Thermo Fisher
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    low molecular weight rna marker - by Bioz Stars, 2020-04
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    90
    Thermo Fisher low range rna molecular weight markers
    Genomic organization and expression strategy of Or-CD15 . ( A ) Screen shot of the RpL23A / Uhg7 region taken from the UCSC genome browser. The RpL23A-RA and Uhg7 transcripts are depicted in blue, the location of Or-CD15 and the newly identified transcript RpL23A-RB are shown in black. Below, sequence conservation as measured by phastcons and the coverage of a multiple genome alignment is indicated; ( B ) Northern blot analysis of adult fruit fly <t>RNA.</t> The genomic position of the utilised probe is outlined in ( A ). The band at about 100 nt corresponds to snoRNA Or-CD15 ; sizes are indicated by an RNA molecular weight ladder (RiboRuler Low Range, Life Technologies); ( C ) <t>PCR</t> experiments using primers annealing to RpL23A exon 1 and Uhg7 exon 4; amplification of the genomic DNA (gDNA) produces the expected fragment of about 2 kb; the same primers used in RT-PCR experiments successfully amplified a fragment of 1.2 kb, representative of the new RpL23A-RB transcript. In this transcript, RpL23A and Uhg7 sequences are fused to each other. On the right, a DNA molecular weight ladder (100bp DNA ladder, New England BioLabs, Ipswich, MA, USA) is given.
    Low Range Rna Molecular Weight Markers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low range rna molecular weight markers/product/Thermo Fisher
    Average 90 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    low range rna molecular weight markers - by Bioz Stars, 2020-04
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    89
    Thermo Fisher 100 nt rna low molecular weight marker
    Genomic organization and expression strategy of Or-CD15 . ( A ) Screen shot of the RpL23A / Uhg7 region taken from the UCSC genome browser. The RpL23A-RA and Uhg7 transcripts are depicted in blue, the location of Or-CD15 and the newly identified transcript RpL23A-RB are shown in black. Below, sequence conservation as measured by phastcons and the coverage of a multiple genome alignment is indicated; ( B ) Northern blot analysis of adult fruit fly <t>RNA.</t> The genomic position of the utilised probe is outlined in ( A ). The band at about 100 nt corresponds to snoRNA Or-CD15 ; sizes are indicated by an RNA molecular weight ladder (RiboRuler Low Range, Life Technologies); ( C ) <t>PCR</t> experiments using primers annealing to RpL23A exon 1 and Uhg7 exon 4; amplification of the genomic DNA (gDNA) produces the expected fragment of about 2 kb; the same primers used in RT-PCR experiments successfully amplified a fragment of 1.2 kb, representative of the new RpL23A-RB transcript. In this transcript, RpL23A and Uhg7 sequences are fused to each other. On the right, a DNA molecular weight ladder (100bp DNA ladder, New England BioLabs, Ipswich, MA, USA) is given.
    100 Nt Rna Low Molecular Weight Marker, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100 nt rna low molecular weight marker/product/Thermo Fisher
    Average 89 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher riboruler low range rna ladder
    Incubation of in vitro transcripts of CRISPR1, 2 and 3 with Cas6-1. ( A ) A total of 2.3 μM of CRISPR1 transcript is cleaved by 6.2 μM Cas6-1 by incubation for 1 h. ( B ) A total of 2 μM of CRISPR2 transcript is cleaved by 6.2 μM Cas6-1 by incubation for 1 h. ( C ) A total of 3.2 μM of CRISPR3 transcript is not cleaved by 6.5 μM Cas6-1 by incubation for 2 h. ( D ) The cleavage of 6.6 μM of the shorter CRISPR1* in vitro transcript by 5.8 μM Cas6-1 monitored over a time of 2 h 20 min. Since no finally mature crRNA ( 17 ) was detected, the in vivo presence of an unknown ribonuclease is suggested. All reactions were performed at 37°C in a reaction volume of 5 μl. <t>RNA</t> was separated by 8 M urea 10% PAGE and bands were visualized by ethidium bromide staining. Diamonds represent repeats and rectangles spacer sequences. Transcripts were not gel purified, explaining the appearance of fragments smaller than the full length transcripts of CRISPR1 and 2 in absence of Cas6-1. Eb1, elution buffer 1 used as negative control. Molecular markers: MN, Low Range ssRNA Ladder (NEB); MF, <t>RiboRuler</t> Low Range RNA Ladder (Thermo Fisher Scientific).
    Riboruler Low Range Rna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/riboruler low range rna ladder/product/Thermo Fisher
    Average 99 stars, based on 99 article reviews
    Price from $9.99 to $1999.99
    riboruler low range rna ladder - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher generuler ultra low range dna ladder
    Incubation of in vitro transcripts of CRISPR1, 2 and 3 with Cas6-1. ( A ) A total of 2.3 μM of CRISPR1 transcript is cleaved by 6.2 μM Cas6-1 by incubation for 1 h. ( B ) A total of 2 μM of CRISPR2 transcript is cleaved by 6.2 μM Cas6-1 by incubation for 1 h. ( C ) A total of 3.2 μM of CRISPR3 transcript is not cleaved by 6.5 μM Cas6-1 by incubation for 2 h. ( D ) The cleavage of 6.6 μM of the shorter CRISPR1* in vitro transcript by 5.8 μM Cas6-1 monitored over a time of 2 h 20 min. Since no finally mature crRNA ( 17 ) was detected, the in vivo presence of an unknown ribonuclease is suggested. All reactions were performed at 37°C in a reaction volume of 5 μl. <t>RNA</t> was separated by 8 M urea 10% PAGE and bands were visualized by ethidium bromide staining. Diamonds represent repeats and rectangles spacer sequences. Transcripts were not gel purified, explaining the appearance of fragments smaller than the full length transcripts of CRISPR1 and 2 in absence of Cas6-1. Eb1, elution buffer 1 used as negative control. Molecular markers: MN, Low Range ssRNA Ladder (NEB); MF, <t>RiboRuler</t> Low Range RNA Ladder (Thermo Fisher Scientific).
    Generuler Ultra Low Range Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/generuler ultra low range dna ladder/product/Thermo Fisher
    Average 99 stars, based on 134 article reviews
    Price from $9.99 to $1999.99
    generuler ultra low range dna ladder - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Electrophoretic analysis of partial pep A gene (596 bp) of B. pseudomallei digested with Stu I and Hinc II restriction analysis. (12% polyacrylamide gel, 1X TBE buffer, 8 V/cm, 130 min); Lane M- O’GeneRuler™ ultra low range DNA ladder; Lane 1- B. pseudomallei NCTC 13178; Lane 2- B. pseudomallei ATCC 23343; Lane 3- Type I; Lane 4- Type II; Lane 5- Type III.

    Journal: BMC Microbiology

    Article Title: Enzymatic and molecular characterisation of leucine aminopeptidase of Burkholderia pseudomallei

    doi: 10.1186/1471-2180-13-110

    Figure Lengend Snippet: Electrophoretic analysis of partial pep A gene (596 bp) of B. pseudomallei digested with Stu I and Hinc II restriction analysis. (12% polyacrylamide gel, 1X TBE buffer, 8 V/cm, 130 min); Lane M- O’GeneRuler™ ultra low range DNA ladder; Lane 1- B. pseudomallei NCTC 13178; Lane 2- B. pseudomallei ATCC 23343; Lane 3- Type I; Lane 4- Type II; Lane 5- Type III.

    Article Snippet: An O’GeneRuler™ Ultra Low Range DNA ladder (Fermentas, Lithuania) was used as molecular weight marker.

    Techniques:

    In vivo expression and SL trans -splicing of a chimeric TnI/β-gal mRNA from a TnI/β-gal gene construct lacking the SL sequence. ( A ) Expression of β-gal, revealed by X-Gal staining (blue), in tail muscle of embryos 12 h following introduction of CiTnILacZ(−1.5) DNA into zygotes by electroporation. ( B ) RT–PCR amplification of β-gal mRNA with β-gal-specific leftward priming, and rightward priming with the SL primer. (Lane 1 ) Size markers (MBI Fermentas ladder mix, 10–0.1 kb; top visible band, 3 kb, bottom visible band 500 bp). (Lanes 2,3 ) RT–PCR products from two different batches of transfected embryos. The template in lane 4 was tRNA, used as a carrier in embryo RNA isolations. A 550-bp product, the size predicted for SL-ended β-gal mRNA, was produced from both embryo batches. [Production of this product required the presence of both primers and did not occur when CiTnILacZ(−1.5) plasmid DNA was used as the amplification template. An additional product of ∼400 bp seen in lanes 2 and 3 required only the β-gal-specific primer and was produced in control amplifications of CiTnILacZ(−1.5) plasmid DNA; it apparently results from rightward mis-priming by the β-gal-specific primer upstream of its normal leftward priming site.] ( C ) DNA sequence of 550-bp RT–PCR product. The 550-bp product (as in B ) was recovered and sequenced using the SL primer ( right ) and β-gal-specific primer ( left ). The leftward sequence confirmed the presence of the SL primer (bold) immediately upstream of TnI mRNA nucleotide 17. Not shown is the Bam HI site engineered at the 5′-end of the SL primer. Sequences deriving from the β-gal reporter gene are shown in lower case.

    Journal: Genes & Development

    Article Title: mRNA 5?-leader trans-splicing in the chordates

    doi: 10.1101/gad.865401

    Figure Lengend Snippet: In vivo expression and SL trans -splicing of a chimeric TnI/β-gal mRNA from a TnI/β-gal gene construct lacking the SL sequence. ( A ) Expression of β-gal, revealed by X-Gal staining (blue), in tail muscle of embryos 12 h following introduction of CiTnILacZ(−1.5) DNA into zygotes by electroporation. ( B ) RT–PCR amplification of β-gal mRNA with β-gal-specific leftward priming, and rightward priming with the SL primer. (Lane 1 ) Size markers (MBI Fermentas ladder mix, 10–0.1 kb; top visible band, 3 kb, bottom visible band 500 bp). (Lanes 2,3 ) RT–PCR products from two different batches of transfected embryos. The template in lane 4 was tRNA, used as a carrier in embryo RNA isolations. A 550-bp product, the size predicted for SL-ended β-gal mRNA, was produced from both embryo batches. [Production of this product required the presence of both primers and did not occur when CiTnILacZ(−1.5) plasmid DNA was used as the amplification template. An additional product of ∼400 bp seen in lanes 2 and 3 required only the β-gal-specific primer and was produced in control amplifications of CiTnILacZ(−1.5) plasmid DNA; it apparently results from rightward mis-priming by the β-gal-specific primer upstream of its normal leftward priming site.] ( C ) DNA sequence of 550-bp RT–PCR product. The 550-bp product (as in B ) was recovered and sequenced using the SL primer ( right ) and β-gal-specific primer ( left ). The leftward sequence confirmed the presence of the SL primer (bold) immediately upstream of TnI mRNA nucleotide 17. Not shown is the Bam HI site engineered at the 5′-end of the SL primer. Sequences deriving from the β-gal reporter gene are shown in lower case.

    Article Snippet: RNA markers were the MBI Fermentas low-molecular weight RNA marker set.

    Techniques: In Vivo, Expressing, Construct, Sequencing, Staining, Electroporation, Reverse Transcription Polymerase Chain Reaction, Amplification, Transfection, Produced, Plasmid Preparation

    Genomic organization and expression strategy of Or-CD15 . ( A ) Screen shot of the RpL23A / Uhg7 region taken from the UCSC genome browser. The RpL23A-RA and Uhg7 transcripts are depicted in blue, the location of Or-CD15 and the newly identified transcript RpL23A-RB are shown in black. Below, sequence conservation as measured by phastcons and the coverage of a multiple genome alignment is indicated; ( B ) Northern blot analysis of adult fruit fly RNA. The genomic position of the utilised probe is outlined in ( A ). The band at about 100 nt corresponds to snoRNA Or-CD15 ; sizes are indicated by an RNA molecular weight ladder (RiboRuler Low Range, Life Technologies); ( C ) PCR experiments using primers annealing to RpL23A exon 1 and Uhg7 exon 4; amplification of the genomic DNA (gDNA) produces the expected fragment of about 2 kb; the same primers used in RT-PCR experiments successfully amplified a fragment of 1.2 kb, representative of the new RpL23A-RB transcript. In this transcript, RpL23A and Uhg7 sequences are fused to each other. On the right, a DNA molecular weight ladder (100bp DNA ladder, New England BioLabs, Ipswich, MA, USA) is given.

    Journal: Non-Coding RNA

    Article Title: Unusual Novel SnoRNA-Like RNAs in Drosophila melanogaster

    doi: 10.3390/ncrna1020139

    Figure Lengend Snippet: Genomic organization and expression strategy of Or-CD15 . ( A ) Screen shot of the RpL23A / Uhg7 region taken from the UCSC genome browser. The RpL23A-RA and Uhg7 transcripts are depicted in blue, the location of Or-CD15 and the newly identified transcript RpL23A-RB are shown in black. Below, sequence conservation as measured by phastcons and the coverage of a multiple genome alignment is indicated; ( B ) Northern blot analysis of adult fruit fly RNA. The genomic position of the utilised probe is outlined in ( A ). The band at about 100 nt corresponds to snoRNA Or-CD15 ; sizes are indicated by an RNA molecular weight ladder (RiboRuler Low Range, Life Technologies); ( C ) PCR experiments using primers annealing to RpL23A exon 1 and Uhg7 exon 4; amplification of the genomic DNA (gDNA) produces the expected fragment of about 2 kb; the same primers used in RT-PCR experiments successfully amplified a fragment of 1.2 kb, representative of the new RpL23A-RB transcript. In this transcript, RpL23A and Uhg7 sequences are fused to each other. On the right, a DNA molecular weight ladder (100bp DNA ladder, New England BioLabs, Ipswich, MA, USA) is given.

    Article Snippet: The size of RNAs was determined by using high range and low range RNA molecular weight markers (Life Technologies) Quantitative real-time RT-PCR (qRT-PCR) experiments were performed in triplicate using iQ5 Multicolor Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) as previously described [ ].

    Techniques: Expressing, Sequencing, Northern Blot, Molecular Weight, Polymerase Chain Reaction, Amplification, Reverse Transcription Polymerase Chain Reaction

    Incubation of in vitro transcripts of CRISPR1, 2 and 3 with Cas6-1. ( A ) A total of 2.3 μM of CRISPR1 transcript is cleaved by 6.2 μM Cas6-1 by incubation for 1 h. ( B ) A total of 2 μM of CRISPR2 transcript is cleaved by 6.2 μM Cas6-1 by incubation for 1 h. ( C ) A total of 3.2 μM of CRISPR3 transcript is not cleaved by 6.5 μM Cas6-1 by incubation for 2 h. ( D ) The cleavage of 6.6 μM of the shorter CRISPR1* in vitro transcript by 5.8 μM Cas6-1 monitored over a time of 2 h 20 min. Since no finally mature crRNA ( 17 ) was detected, the in vivo presence of an unknown ribonuclease is suggested. All reactions were performed at 37°C in a reaction volume of 5 μl. RNA was separated by 8 M urea 10% PAGE and bands were visualized by ethidium bromide staining. Diamonds represent repeats and rectangles spacer sequences. Transcripts were not gel purified, explaining the appearance of fragments smaller than the full length transcripts of CRISPR1 and 2 in absence of Cas6-1. Eb1, elution buffer 1 used as negative control. Molecular markers: MN, Low Range ssRNA Ladder (NEB); MF, RiboRuler Low Range RNA Ladder (Thermo Fisher Scientific).

    Journal: Nucleic Acids Research

    Article Title: Structural constraints and enzymatic promiscuity in the Cas6-dependent generation of crRNAs

    doi: 10.1093/nar/gkw786

    Figure Lengend Snippet: Incubation of in vitro transcripts of CRISPR1, 2 and 3 with Cas6-1. ( A ) A total of 2.3 μM of CRISPR1 transcript is cleaved by 6.2 μM Cas6-1 by incubation for 1 h. ( B ) A total of 2 μM of CRISPR2 transcript is cleaved by 6.2 μM Cas6-1 by incubation for 1 h. ( C ) A total of 3.2 μM of CRISPR3 transcript is not cleaved by 6.5 μM Cas6-1 by incubation for 2 h. ( D ) The cleavage of 6.6 μM of the shorter CRISPR1* in vitro transcript by 5.8 μM Cas6-1 monitored over a time of 2 h 20 min. Since no finally mature crRNA ( 17 ) was detected, the in vivo presence of an unknown ribonuclease is suggested. All reactions were performed at 37°C in a reaction volume of 5 μl. RNA was separated by 8 M urea 10% PAGE and bands were visualized by ethidium bromide staining. Diamonds represent repeats and rectangles spacer sequences. Transcripts were not gel purified, explaining the appearance of fragments smaller than the full length transcripts of CRISPR1 and 2 in absence of Cas6-1. Eb1, elution buffer 1 used as negative control. Molecular markers: MN, Low Range ssRNA Ladder (NEB); MF, RiboRuler Low Range RNA Ladder (Thermo Fisher Scientific).

    Article Snippet: Before loading onto denaturing 8 M urea 10% PAA gels, reactions were incubated at 95°C for 5 min. As size markers served the Low Range ssRNA Ladder from NEB (MN ), the RiboRuler Low Range RNA Ladder from Thermo Fisher Scientific (MF ) and the Low Molecular Weight Marker from Affymetrix (MA ).

    Techniques: Incubation, In Vitro, In Vivo, Polyacrylamide Gel Electrophoresis, Staining, Purification, Negative Control