Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: NIPP-induced alterations in extracellular H 2 O 2 levels, intracellular ROS production, and MMP in tumor cells. ( A ): H 2 O 2 level in the cell culture medium RPMI 1640 and DMEM/F12, and PBS after treatment with NIPP ( a ); NO 2 − level in the cell culture medium RPMI 1640 and DMEM/F12, and PBS after treatment with NIPP ( b ); NO 3 − level in the cell culture medium RPMI 1640 and DMEM/F12, and PBS after treatment with NIPP ( c ). ( B ): ROS level inside the tumor cells SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), MDA-MB-231 ( f ) cells, after treatment with the NIPP in 30 min ( n = 6). All data are normalized to the control group; ( C ): Mitochondria membrane potential relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), MDA-MB-231 ( f ) cells after the exposure of NIPP 24 h, 48 h, and 72 h. Every experiment was replicated at least 3 times. All data are normalized to the control group. *** p < 0.001, **: p < 0.01, *: p < 0.05.

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Cell Culture, Control, Membrane

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: NIPP inhibits the proliferation of tumor cells. ( A ): Cell growth curves for SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells. The groups were categorized based on their distance from NIPP to the medium surface: the control group, 1 cm group, 3 cm group, and 6 cm group. All groups, except the control group, were exposed to NIPP for 4 min. Each experiment was conducted at least three times. ( B ): Visualization of NIPP treatment tumor cells with different distances. ( C ): Cell growth curves for SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells. The groups were organized based on the treatment time of NIPP for the tumor cells: the control group, 1 min group, 2 min group, and 4 min group. Except for the control group, the distance of NIPP from the culture medium was 1 cm for all groups. ( D ): Visualization of NIPP treatment tumor cells with different duration. Each experiment was repeated at least three times. ( E ): Wound-healing Test of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells at 0 and 24 h. Each experimental group was compared with the control group.

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Control

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: NIPP treatment alters the cytoskeletal organization of tumor cells and glucose consumption in tumor cell culture medium. ( A ): Immunofluorescence of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells. Green represents F-actin, and Blue represents the Nucleus. Magnification 20×. Next row of pictures are partial screenshots from the previous row. ( B ): Glucose relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells after the exposure to NIPP for 24 h, 48 h, and 72 h. ( C ): Lactate relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells after the exposure to NIPP for 24 h, 48 h, and 72 h. Every experiment was replicated at least 3 times. Each experimental group was compared with the control group. ***: p < 0.001, **: p < 0.01, *: p < 0.05.

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Cell Culture, Immunofluorescence, Control

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: NIPP increases LDH release in tumor cell cultures and induced no significante alterations of SOD levels in tumor cell. ( A ): LDH relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells after the exposure to NIPP for 30 min and 24 h. ( B ): SOD relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells after the exposure to NIPP for 24 h. Every experiment was replicated at least 3 times. Each experimental group was compared with the control group. ***: p < 0.001, **: p < 0.01, *: p < 0.05.

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Control

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: ( A ): Quantification of HSP27 relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7, and MDA-MB-231 cells after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. The uncropped bolts are shown in .

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Expressing, Western Blot, Control

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: ( A ): Quantification of HSP40 relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7, and MDA-MB-231 cells after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. **: p < 0.01, *: p < 0.05. The uncropped bolts are shown in .

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Expressing, Western Blot, Control

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: ( A ): Quantification of HSP70 relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7, and MDA-MB-231 cells after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. **: p < 0.01, *: p < 0.05. The uncropped bolts are shown in .

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Expressing, Western Blot, Control

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: ( A ): Quantification of HSP90α relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7 cells, and MDA-MB-231 after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. They were statistically evaluated with Student t -test. **: p < 0.01, *: p < 0.05. The uncropped bolts are shown in .

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Expressing, Western Blot, Control

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: ( A ): Quantification of HSP90β relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7 cells, and MDA-MB-231 after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. They were statistically evaluated with Student t -test. **: p < 0.01, *: p < 0.05. The uncropped bolts are shown in .

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Expressing, Western Blot, Control

Journal: Scientific Reports

Article Title: Dynamic interplay between sortilin and syndecan-1 contributes to prostate cancer progression

doi: 10.1038/s41598-023-40347-7

Figure Lengend Snippet: Sortilin controls glucose uptake in PCa cells and glucose alters expression of sugar metabolism-related proteins. ( a ) Sortilin (SORT1) and syndecan-1 (SDC1)-interacting proteins, which are implicated in PCa development and progression. Red nodes; SORT and SDC1, gray nodes; proteins crucial for PCa metabolism relevant to this study. Line thickness indicates the strength of data support. ( b ) diagram showing involvement of sortilin (SORT) in GLUT4 and potentially GLUT1 pathways. ( c ) Expression of GLUT1, ( d ) GLUT4 and ( e ) glucose uptake in LNCaP cells after sortilin knockdown (siSORT) as compared to scrambled siRNA (Scr siRNA). ( f ) Expression of SORT, GLUT1 and GLUT4 in different glucose concentrations in prostate cell lines. Western blotting signal was normalised using total protein staining. Data are presented as mean ± SD, in ( c,d , f ) n = 3 (independent experiments), in ( e ) n = 5 (independent experiments), in ( c–e ), one-sample t-test, in ( f ) one-way ANOVA test, where samples were normalized and compared to 5.5 mM glucose.

Article Snippet: Human prostate cell PNT2 (ECACC 95012613), LNCaP (clone FCG, ECACC 89110211) and PC3 (ECACC 90112714) were obtained from the European Collection of Authenticated Cell Cultures (ECACC) from CellBank Australia (Children's Medical Research Institute, Westmead, NSW, Australia).

Techniques: Expressing, Knockdown, Western Blot, Staining

Journal: Scientific Reports

Article Title: Dynamic interplay between sortilin and syndecan-1 contributes to prostate cancer progression

doi: 10.1038/s41598-023-40347-7

Figure Lengend Snippet: Sortilin (SORT) interacts with progranulin (PRGN) and LPL in PCa cells and PRGN is secreted by aggressive PC3 cells. ( a ) Representative confocal images showing co-labelling of cells with anti-PRGN (red) and anti-SORT (green) antibodies. Scale bars; 10 µm. ( b ) Quantification of colocalisation between PRGN and SORT. ( c ) Amount of PRGN in conditioned media (CM) and corresponding cell lysates with quantification of band densities and ratio of PRGN in CM to PRGN in cell lysates. ( d ) Endogenous SORT was immunoprecipitated (IP) with anti-SORT antibodies and PRGN was detected by Western blotting. Input; cell lysate. ( e ) Representative confocal images showing co-labelling of cells with anti-LPL (red) and anti-SORT (green) antibodies. Scale bars; 10 µm. ( f ) Quantification of colocalisation between LPL and SORT. ( g ) Amount of LPL in CM and corresponding cell lysates with quantification of band densities in cell lysates. In ( c ) and ( g ) PNT2 bands from the same membrane were shifted towards LNCaP and PC3 bands. Western blotting signal was normalised using total protein staining. ( h ) Endogenous SORT was immunoprecipitated (IP) with anti-SORT antibodies and LPL was detected by Western blotting. Input; cell lysate. Data are presented as mean ± SD, in ( b , f ) n = 10 representative ROIs, in ( c , g ) n = 3 (independent experiments), one-way ANOVA.

Article Snippet: Human prostate cell PNT2 (ECACC 95012613), LNCaP (clone FCG, ECACC 89110211) and PC3 (ECACC 90112714) were obtained from the European Collection of Authenticated Cell Cultures (ECACC) from CellBank Australia (Children's Medical Research Institute, Westmead, NSW, Australia).

Techniques: Immunoprecipitation, Western Blot, Membrane, Staining