Journal: Frontiers in bioscience (Landmark edition)

Article Title: Differential Gene Expression Signatures and Cellular Signaling Pathways induced by Lamin A/C Transcript Variants in MCF7 Cell Line.

doi: 10.31083/j.fbl2806113

Figure Lengend Snippet: Fig. 5. Lamin A/C transcript variants expression in MCF7/ADR cell line and the effect of Lamin A∆10 inhibition on cell survival

Article Snippet: The GFP-tagged lamin A∆50 was obtained fromAddgene.org (Plasmid #17653), while the Cterminal GFP-tagged lamin A, lamin C, and lamin A∆10 were purchased from OriGene (Rockville, MD, USA).

Techniques: Expressing, Inhibition

Journal: PloS one

Article Title: Whole-exome sequencing to identify a novel LMNA gene mutation associated with inherited cardiac conduction disease.

doi: 10.1371/journal.pone.0083322

Figure Lengend Snippet: Figure 4. Expression of lamin A/C in cultured atrial cardiomyocytes. HL-1 atrial cardiomyocytes transfected with wild-type lamin A (A) and lamin C (B) in fluorescent expression vectors, showing the cell nucleus with homogenous nuclear veil. HL-1 atrial cardiomyocytes transfected with p.G232V mutant lamin A (C) and lamin C (D), showing the cell nucleus with aggregates.

Article Snippet: To characterize the consequences of the LMNA mutation at the cellular level, transient cell transfections were performed in HL-1 atrial cardiomyocytes with WT or mutated lamin A (NM_170707.3) and lamin C (NM_005572.3) mRNA expressed as fusions to the C-terminus of green fluorescent protein ( pCMV6-AC-GFP, RG204970 and RG201809 for Lamin A and Lamin C repectively, OriGene Technologies).

Techniques: Expressing, Cell Culture, Transfection, Mutagenesis