Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Paeoniflorin Attenuates Dexamethasone-Induced Apoptosis of Osteoblast Cells and Promotes Bone Formation via Regulating AKT/mTOR/Autophagy Signaling Pathway

doi: 10.1155/2021/6623464

Figure Lengend Snippet: Effect of paeoniflorin on DEX-induced apoptosis in MC3T3-E1 cells. (a, b) The extent of apoptosis of MC3T3-E1 cells was detected by flow cytometry after Annexin V-FITC/PI double staining. The apoptotic rate was measured by flow cytometric analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX. (c) MC3T3-E1 cells were stained by Hoechst to show the extent of apoptosis after interventions of DEX and paeoniflorin. (d) The expressions of osteogenic protein-Runx2 (runt-related transcription factor2) and apoptotic proteins such as Bcl-2 (B cell leukemia 2) and Bax (BCL2-associated X protein) were measured by western blot. ∗ P < 0.05, ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX; ## P < 0.01 vs. DEX. (e) Protein levels of caspase-3 and cleaved caspase-3 were determined by western blot analysis, shown in quantitative analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX.

Article Snippet: For Hoechst staining, 10 μ L Hoechst live cell staining solution (Beyotime Institute of Biotechnology) was added to the culture medium and mixed gently.

Techniques: Flow Cytometry, Double Staining, Control, Staining, Western Blot

Journal: Cell Death & Disease

Article Title: Protein synthesis inhibition and loss of homeostatic functions in astrocytes from an Alzheimer’s disease mouse model: a role for ER-mitochondria interaction

doi: 10.1038/s41419-022-05324-4

Figure Lengend Snippet: a Cells were pulsed with puromycin (4 µM, 1.5 h), fixed and analysed by IF with anti-puromycin antibody (green) and with DAPI to stain nuclei (blue). Images were acquired with Leica Thunder imager 3D live cell microscope, scale bar = 25 µm. Data are expressed as mean ± SEM, WT-iAstro cells n = 40, 3Tg-iAstro cells n = 40, from 4 independent experiments; **** p < 0.05 by unpaired t -test. b WB with anti-puromycin antibody and ponceau staining on cells treated with 4 µM puromycin. Data are expressed as mean ± SEM of 12 independent experiments; **** p < 0.0001 by unpaired t -test.

Article Snippet: Images were acquired using a FV-1000 Olympus laser confocal scanning system, Zeiss 710 confocal laser scanning microscope, Leica SP8 LIGHTNING Confocal Microscope imaging systems and Leica Thunder imager 3D live cell.

Techniques: Staining, Microscopy