liquid 7h9 medium Difco Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    MiddleBrook Pharmaceuticals medium
    Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/medium/product/MiddleBrook Pharmaceuticals
    Average 93 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    medium - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    96
    Difco 7h9 medium
    —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid <t>7H9</t> medium supplemented with 0.05% Tween 80, ADC supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).
    7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 96/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h9 medium/product/Difco
    Average 96 stars, based on 248 article reviews
    Price from $9.99 to $1999.99
    7h9 medium - by Bioz Stars, 2020-02
    96/100 stars
      Buy from Supplier

    94
    Difco 7h9 liquid medium
    Effects of pH on the rate of inactivation of M. gordonae . Experimental conditions were a temperature of 25°C and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook <t>7H9-Tween</t> medium. No, initial number of CFU; N, number of CFU at each time point. Experiments were conducted with different phosphate (0.05 M) buffers within the pH range of 6.0 to 8.0. Slopes were calculated as follows: pH 6, y = 0.11 x ; pH 7, y = 0.09 x ; and pH 8, y = 0.02 x . R 2 , correlation coefficient values.
    7h9 Liquid Medium, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h9 liquid medium/product/Difco
    Average 94 stars, based on 235 article reviews
    Price from $9.99 to $1999.99
    7h9 liquid medium - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    97
    Difco middlebrook 7h9 medium
    M. smegmatis Δ cpa shows normal growth behavior under standard culture conditions but displays a slight growth defect during carbon starvation. ( A ) Msm parent and knockout cells were cultured in <t>Middlebrook</t> <t>7H9</t> medium at 37°C and cell density was measured at 600 nm at the indicated time points. Both strains showed identical growth behavior indicating that Cpa is dispensable during standard cell culture conditions. ( B ) cpa -knockout cells show impaired growth in the same medium devoid of glycerol as a main carbon source. Both strains were cultured in a minimal medium in absence of glycerol at 37°C. Both growth curves are representative of three or more independent experiments with the mean values ± SD plotted.
    Middlebrook 7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 97/100, based on 835 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook 7h9 medium/product/Difco
    Average 97 stars, based on 835 article reviews
    Price from $9.99 to $1999.99
    middlebrook 7h9 medium - by Bioz Stars, 2020-02
    97/100 stars
      Buy from Supplier

    94
    Becton Dickinson middlebrook 7h9 medium
    M. smegmatis Δ cpa shows normal growth behavior under standard culture conditions but displays a slight growth defect during carbon starvation. ( A ) Msm parent and knockout cells were cultured in <t>Middlebrook</t> <t>7H9</t> medium at 37°C and cell density was measured at 600 nm at the indicated time points. Both strains showed identical growth behavior indicating that Cpa is dispensable during standard cell culture conditions. ( B ) cpa -knockout cells show impaired growth in the same medium devoid of glycerol as a main carbon source. Both strains were cultured in a minimal medium in absence of glycerol at 37°C. Both growth curves are representative of three or more independent experiments with the mean values ± SD plotted.
    Middlebrook 7h9 Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook 7h9 medium/product/Becton Dickinson
    Average 94 stars, based on 259 article reviews
    Price from $9.99 to $1999.99
    middlebrook 7h9 medium - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    99
    Becton Dickinson 7h9 liquid medium
    M. smegmatis Δ cpa shows normal growth behavior under standard culture conditions but displays a slight growth defect during carbon starvation. ( A ) Msm parent and knockout cells were cultured in <t>Middlebrook</t> <t>7H9</t> medium at 37°C and cell density was measured at 600 nm at the indicated time points. Both strains showed identical growth behavior indicating that Cpa is dispensable during standard cell culture conditions. ( B ) cpa -knockout cells show impaired growth in the same medium devoid of glycerol as a main carbon source. Both strains were cultured in a minimal medium in absence of glycerol at 37°C. Both growth curves are representative of three or more independent experiments with the mean values ± SD plotted.
    7h9 Liquid Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h9 liquid medium/product/Becton Dickinson
    Average 99 stars, based on 110 article reviews
    Price from $9.99 to $1999.99
    7h9 liquid medium - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    84
    Difco 7h10 solid medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    7h10 Solid Medium, supplied by Difco, used in various techniques. Bioz Stars score: 84/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 solid medium/product/Difco
    Average 84 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    7h10 solid medium - by Bioz Stars, 2020-02
    84/100 stars
      Buy from Supplier

    99
    MiddleBrook Pharmaceuticals 7h9 liquid medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    7h9 Liquid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 99/100, based on 622 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h9 liquid medium/product/MiddleBrook Pharmaceuticals
    Average 99 stars, based on 622 article reviews
    Price from $9.99 to $1999.99
    7h9 liquid medium - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    84
    Difco 7h9 liquid growth medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    7h9 Liquid Growth Medium, supplied by Difco, used in various techniques. Bioz Stars score: 84/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h9 liquid growth medium/product/Difco
    Average 84 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    7h9 liquid growth medium - by Bioz Stars, 2020-02
    84/100 stars
      Buy from Supplier

    99
    MiddleBrook Pharmaceuticals middlebrook 7h9 medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    Middlebrook 7h9 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 99/100, based on 2844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook 7h9 medium/product/MiddleBrook Pharmaceuticals
    Average 99 stars, based on 2844 article reviews
    Price from $9.99 to $1999.99
    middlebrook 7h9 medium - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    75
    Difco middelbrook 7h9 medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    Middelbrook 7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 75/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middelbrook 7h9 medium/product/Difco
    Average 75 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    middelbrook 7h9 medium - by Bioz Stars, 2020-02
    75/100 stars
      Buy from Supplier

    79
    MiddleBrook Pharmaceuticals dubos oleic albumin complex enriched middlebrook 7h9 liquid medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    Dubos Oleic Albumin Complex Enriched Middlebrook 7h9 Liquid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dubos oleic albumin complex enriched middlebrook 7h9 liquid medium/product/MiddleBrook Pharmaceuticals
    Average 79 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    dubos oleic albumin complex enriched middlebrook 7h9 liquid medium - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    95
    Difco middlebrook 7h9
    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on <t>Middlebrook</t> 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook <t>7H9</t> liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .
    Middlebrook 7h9, supplied by Difco, used in various techniques. Bioz Stars score: 95/100, based on 320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook 7h9/product/Difco
    Average 95 stars, based on 320 article reviews
    Price from $9.99 to $1999.99
    middlebrook 7h9 - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    77
    Difco mycomedia
    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on <t>Middlebrook</t> 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook <t>7H9</t> liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .
    Mycomedia, supplied by Difco, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mycomedia/product/Difco
    Average 77 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    mycomedia - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    Image Search Results


    —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid 7H9 medium supplemented with 0.05% Tween 80, ADC supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).

    Journal: Genome Biology and Evolution

    Article Title: Unexpected Genomic and Phenotypic Diversity of Mycobacterium africanum Lineage 5 Affects Drug Resistance, Protein Secretion, and Immunogenicity

    doi: 10.1093/gbe/evy145

    Figure Lengend Snippet: —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid 7H9 medium supplemented with 0.05% Tween 80, ADC supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).

    Article Snippet: Cultivation of all isolates ( , online) was performed on 7H11 solid medium with OADC supplement (Difco), or in liquid 7H9 medium supplemented with ADC (Difco), 0.05% Tween 80, and 0.2% w/v pyruvate ( ).

    Techniques:

    Effects of pH on the rate of inactivation of M. gordonae . Experimental conditions were a temperature of 25°C and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. No, initial number of CFU; N, number of CFU at each time point. Experiments were conducted with different phosphate (0.05 M) buffers within the pH range of 6.0 to 8.0. Slopes were calculated as follows: pH 6, y = 0.11 x ; pH 7, y = 0.09 x ; and pH 8, y = 0.02 x . R 2 , correlation coefficient values.

    Journal: Applied and Environmental Microbiology

    Article Title: Chlorine Disinfection of Atypical Mycobacteria Isolated from a Water Distribution System

    doi: 10.1128/AEM.68.3.1025-1032.2002

    Figure Lengend Snippet: Effects of pH on the rate of inactivation of M. gordonae . Experimental conditions were a temperature of 25°C and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. No, initial number of CFU; N, number of CFU at each time point. Experiments were conducted with different phosphate (0.05 M) buffers within the pH range of 6.0 to 8.0. Slopes were calculated as follows: pH 6, y = 0.11 x ; pH 7, y = 0.09 x ; and pH 8, y = 0.02 x . R 2 , correlation coefficient values.

    Article Snippet: Cells were grown in Middlebrook 7H9 liquid medium (Difco Laboratories, West Molesley, Surrey, United Kingdom) containing 10% (vol/vol) oleic acid-albumin enrichment and 0.05% (vol/vol) Tween 80 at their optimal growth temperature (30 or 37°C, depending on the strain) in a rotary shaker (120 rpm).

    Techniques: Concentration Assay

    Effects of temperature on chlorine inactivation of M. gordonae . Experimental conditions were pH 7 and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. The chlorine susceptibility of M. gordonae was analyzed at 4, 16, and 25°C. No, initial number of CFU; N, number of CFU at each time point. Symbols: ▪, 4°C ( y = 0.01 x ); ▴, 16°C ( y = 0.02 x ); ⧫, 25°C ( y = 0.09 x ). R 2 , correlation coefficient values.

    Journal: Applied and Environmental Microbiology

    Article Title: Chlorine Disinfection of Atypical Mycobacteria Isolated from a Water Distribution System

    doi: 10.1128/AEM.68.3.1025-1032.2002

    Figure Lengend Snippet: Effects of temperature on chlorine inactivation of M. gordonae . Experimental conditions were pH 7 and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. The chlorine susceptibility of M. gordonae was analyzed at 4, 16, and 25°C. No, initial number of CFU; N, number of CFU at each time point. Symbols: ▪, 4°C ( y = 0.01 x ); ▴, 16°C ( y = 0.02 x ); ⧫, 25°C ( y = 0.09 x ). R 2 , correlation coefficient values.

    Article Snippet: Cells were grown in Middlebrook 7H9 liquid medium (Difco Laboratories, West Molesley, Surrey, United Kingdom) containing 10% (vol/vol) oleic acid-albumin enrichment and 0.05% (vol/vol) Tween 80 at their optimal growth temperature (30 or 37°C, depending on the strain) in a rotary shaker (120 rpm).

    Techniques: Concentration Assay

    M. smegmatis Δ cpa shows normal growth behavior under standard culture conditions but displays a slight growth defect during carbon starvation. ( A ) Msm parent and knockout cells were cultured in Middlebrook 7H9 medium at 37°C and cell density was measured at 600 nm at the indicated time points. Both strains showed identical growth behavior indicating that Cpa is dispensable during standard cell culture conditions. ( B ) cpa -knockout cells show impaired growth in the same medium devoid of glycerol as a main carbon source. Both strains were cultured in a minimal medium in absence of glycerol at 37°C. Both growth curves are representative of three or more independent experiments with the mean values ± SD plotted.

    Journal: eLife

    Article Title: Cdc48-like protein of actinobacteria (Cpa) is a novel proteasome interactor in mycobacteria and related organisms

    doi: 10.7554/eLife.34055

    Figure Lengend Snippet: M. smegmatis Δ cpa shows normal growth behavior under standard culture conditions but displays a slight growth defect during carbon starvation. ( A ) Msm parent and knockout cells were cultured in Middlebrook 7H9 medium at 37°C and cell density was measured at 600 nm at the indicated time points. Both strains showed identical growth behavior indicating that Cpa is dispensable during standard cell culture conditions. ( B ) cpa -knockout cells show impaired growth in the same medium devoid of glycerol as a main carbon source. Both strains were cultured in a minimal medium in absence of glycerol at 37°C. Both growth curves are representative of three or more independent experiments with the mean values ± SD plotted.

    Article Snippet: Mycobacterium smegmatis mc(2) 155 ( Msm ) was grown in liquid Middlebrook 7H9 medium (Difco) supplemented with 0.025% (w/v) Tween 80 and 0.2% (w/v) glycerol, unless otherwise stated.

    Techniques: Knock-Out, Cell Culture

    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook 7H10 plates and incubated at 37 °C for 21 days.

    Journal: Scientific Reports

    Article Title: Assessing the role of Rv1222 (RseA) as an anti-sigma factor of the Mycobacterium tuberculosis extracytoplasmic sigma factor SigE

    doi: 10.1038/s41598-019-41183-4

    Figure Lengend Snippet: Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook 7H10 plates and incubated at 37 °C for 21 days.

    Article Snippet: M. tuberculosis H37Rv was grown in either Middlebrook 7H9 liquid medium or Middlebrook 7H10 solid medium (Difco) supplemented with 0.05% Tween 80 and ADN (2% glucose, 5% bovine serum albumin, 0.85% NaCl).

    Techniques: Mutagenesis, Incubation

    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on Middlebrook 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .

    Journal: Frontiers in Microbiology

    Article Title: Functional Studies of Five Toxin-Antitoxin Modules in Mycobacterium tuberculosis H37Rv

    doi: 10.3389/fmicb.2016.02071

    Figure Lengend Snippet: Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on Middlebrook 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .

    Article Snippet: M. smegmatis mc2 155 was grown in Middlebrook 7H9 (a liquid medium, Difco) containing 0.2% glycerol and 0.02% Tween 80 or Middlebrook 7H10 (a solid agar medium, Difco) containing 0.2% glycerol.

    Techniques: Transformation Assay, Plasmid Preparation, Incubation