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  • 95
    MiddleBrook Pharmaceuticals medium
    Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco 7h9 medium
    —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid <t>7H9</t> medium supplemented with 0.05% Tween 80, ADC supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).
    7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 95/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Difco 7h9 liquid medium
    Growth of M. smegmatis in various nitrogen sources. Wild-type SMR5 and glnR deletion strain MH1 were grown in the presence of the indicated substances (10 mM final concentration) as sole nitrogen source. Standard <t>7H9</t> medium was used as positive control; 7H9 lacking any nitrogen source as negative control (7H9-N). (A) Putative nitrogen sources deduced from microarray results, (B) putative nitrogen sources used by closely related species.
    7h9 Liquid Medium, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Becton Dickinson 7h9 liquid medium
    Growth of M. smegmatis in various nitrogen sources. Wild-type SMR5 and glnR deletion strain MH1 were grown in the presence of the indicated substances (10 mM final concentration) as sole nitrogen source. Standard <t>7H9</t> medium was used as positive control; 7H9 lacking any nitrogen source as negative control (7H9-N). (A) Putative nitrogen sources deduced from microarray results, (B) putative nitrogen sources used by closely related species.
    7h9 Liquid Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Difco middlebrook 7h9 medium
    Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in <t>Middlebrook</t> <t>7H9</t> medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation
    Middlebrook 7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 95/100, based on 835 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    MiddleBrook Pharmaceuticals 7h9 liquid medium
    Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in <t>Middlebrook</t> <t>7H9</t> medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation
    7h9 Liquid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 99/100, based on 622 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson middlebrook 7h9 medium
    Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in <t>Middlebrook</t> <t>7H9</t> medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation
    Middlebrook 7h9 Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 97/100, based on 259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Difco 7h10 solid medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    7h10 Solid Medium, supplied by Difco, used in various techniques. Bioz Stars score: 91/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Difco 7h9 liquid growth medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    7h9 Liquid Growth Medium, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Difco middelbrook 7h9 medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    Middelbrook 7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook 7h9 medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    Middlebrook 7h9 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 99/100, based on 2844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook 7h9 oadc medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    Middlebrook 7h9 Oadc Medium, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco 7h9 basal liquid medium
    prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook <t>7H9</t> broth with glycerol. The optical density at 600
    7h9 Basal Liquid Medium, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    MiddleBrook Pharmaceuticals middlebrook 7h9 broth liquid medium
    prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook <t>7H9</t> broth with glycerol. The optical density at 600
    Middlebrook 7h9 Broth Liquid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals dubos oleic albumin complex enriched middlebrook 7h9 liquid medium
    prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook <t>7H9</t> broth with glycerol. The optical density at 600
    Dubos Oleic Albumin Complex Enriched Middlebrook 7h9 Liquid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Difco middlebrook 7h9
    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on <t>Middlebrook</t> 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook <t>7H9</t> liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .
    Middlebrook 7h9, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco mycomedia
    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on <t>Middlebrook</t> 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook <t>7H9</t> liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .
    Mycomedia, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals difco middlebrook 7h9
    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on <t>Middlebrook</t> 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook <t>7H9</t> liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .
    Difco Middlebrook 7h9, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Difco 7h9 broth base
    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on <t>Middlebrook</t> 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook <t>7H9</t> liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .
    7h9 Broth Base, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid 7H9 medium supplemented with 0.05% Tween 80, ADC supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).

    Journal: Genome Biology and Evolution

    Article Title: Unexpected Genomic and Phenotypic Diversity of Mycobacterium africanum Lineage 5 Affects Drug Resistance, Protein Secretion, and Immunogenicity

    doi: 10.1093/gbe/evy145

    Figure Lengend Snippet: —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid 7H9 medium supplemented with 0.05% Tween 80, ADC supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).

    Article Snippet: Cultivation of all isolates ( , online) was performed on 7H11 solid medium with OADC supplement (Difco), or in liquid 7H9 medium supplemented with ADC (Difco), 0.05% Tween 80, and 0.2% w/v pyruvate ( ).

    Techniques:

    Growth of M. smegmatis in various nitrogen sources. Wild-type SMR5 and glnR deletion strain MH1 were grown in the presence of the indicated substances (10 mM final concentration) as sole nitrogen source. Standard 7H9 medium was used as positive control; 7H9 lacking any nitrogen source as negative control (7H9-N). (A) Putative nitrogen sources deduced from microarray results, (B) putative nitrogen sources used by closely related species.

    Journal: BMC Research Notes

    Article Title: Nitrogen starvation-induced transcriptome alterations and influence of transcription regulator mutants in Mycobacterium smegmatis

    doi: 10.1186/1756-0500-6-482

    Figure Lengend Snippet: Growth of M. smegmatis in various nitrogen sources. Wild-type SMR5 and glnR deletion strain MH1 were grown in the presence of the indicated substances (10 mM final concentration) as sole nitrogen source. Standard 7H9 medium was used as positive control; 7H9 lacking any nitrogen source as negative control (7H9-N). (A) Putative nitrogen sources deduced from microarray results, (B) putative nitrogen sources used by closely related species.

    Article Snippet: Mycobacterial strains were grown in Middlebrook 7H9 liquid medium (Difco Laboratories; per 900 ml approx.

    Techniques: Concentration Assay, Positive Control, Negative Control, Microarray

    Mass spectrometry analysis of MAs. MAs were extracted from mid-log-phase liquid 7H9 medium cultures. Samples were analyzed via a QTRAP 4000 mass spectrometer. (A) Individual sums of C 26 α-, C 24 α-, C 26 keto-, and C 24 keto-MA profiles in

    Journal: Infection and Immunity

    Article Title: An ethA-ethR-Deficient Mycobacterium bovis BCG Mutant Displays Increased Adherence to Mammalian Cells and Greater Persistence In Vivo, Which Correlate with Altered Mycolic Acid Composition

    doi: 10.1128/IAI.01332-13

    Figure Lengend Snippet: Mass spectrometry analysis of MAs. MAs were extracted from mid-log-phase liquid 7H9 medium cultures. Samples were analyzed via a QTRAP 4000 mass spectrometer. (A) Individual sums of C 26 α-, C 24 α-, C 26 keto-, and C 24 keto-MA profiles in

    Article Snippet: Wild-type (WT) M. bovis BCG (Pasteur strain ATCC 35734) and derivative strains were grown at 37°C in Middlebrook liquid 7H9 medium (Difco) or on 7H11 agar supplemented with ADS (0.5% bovine serum albumin fraction V, 0.2% dextrose, 0.85% saline) enrichment, 0.05% Tween 80, and 0.2% glycerol with appropriate antibiotics (80 μg/ml hygromycin [Roche], 20 μg/ml kanamycin [Sigma]).

    Techniques: Mass Spectrometry

    Expression of S18 mRNAs and proteins in M. tuberculosis H37Rv during growth in 7H9/ADC vs. Sauton's medium

    Journal: Molecular microbiology

    Article Title: Zinc Regulates a Switch between Primary and Alternative S18 Ribosomal Proteins in Mycobacterium tuberculosis

    doi: 10.1111/mmi.13022

    Figure Lengend Snippet: Expression of S18 mRNAs and proteins in M. tuberculosis H37Rv during growth in 7H9/ADC vs. Sauton's medium

    Article Snippet: M. tuberculosis H37Rv was grown either in Middlebrook 7H9 liquid culture medium (Difco) supplemented with ADC (0.5% albumin, 0.2% glucose, 0.085% NaCl and 0.05% Tween 80) or Sauton's medium prepared without zinc salts (0.05% KH2 PO4 , 0.05% MgSO4 7H2 O, 0.2% citric acid, 0.005% ferric ammonium citrate, 6% glycerol, 0.4% Asparagine, 0.05% Tween 80, pH 7.4).

    Techniques: Expressing

    Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in Middlebrook 7H9 medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation

    Journal:

    Article Title: A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿

    doi: 10.1128/JB.00257-07

    Figure Lengend Snippet: Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in Middlebrook 7H9 medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation

    Article Snippet: M. smegmatis mc2 155 was grown in liquid cultures using Middlebrook 7H9 medium (Difco) supplemented with 0.2% glycerol and 0.05% Tween 80 or minimal Hartmans-de Bont (HB) medium ( ) at 37°C.

    Techniques:

    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook 7H10 plates and incubated at 37 °C for 21 days.

    Journal: Scientific Reports

    Article Title: Assessing the role of Rv1222 (RseA) as an anti-sigma factor of the Mycobacterium tuberculosis extracytoplasmic sigma factor SigE

    doi: 10.1038/s41598-019-41183-4

    Figure Lengend Snippet: Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook 7H10 plates and incubated at 37 °C for 21 days.

    Article Snippet: M. tuberculosis H37Rv was grown in either Middlebrook 7H9 liquid medium or Middlebrook 7H10 solid medium (Difco) supplemented with 0.05% Tween 80 and ADN (2% glucose, 5% bovine serum albumin, 0.85% NaCl).

    Techniques: Mutagenesis, Incubation

    prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook 7H9 broth with glycerol. The optical density at 600

    Journal: Journal of Bacteriology

    Article Title: The prrAB Two-Component System Is Essential for Mycobacterium tuberculosis Viability and Is Induced under Nitrogen-Limiting Conditions

    doi: 10.1128/JB.06258-11

    Figure Lengend Snippet: prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook 7H9 broth with glycerol. The optical density at 600

    Article Snippet: M. tuberculosis H37Rv cultures were grown at 37°C in Middlebrook 7H9 basal liquid medium (Difco) supplemented with 10% ADS (0.5% bovine serum albumin fraction V, 0.2% dextrose, 140 mM NaCl) enrichment and 0.05% Tween 80 (herein described as supplemented Middlebrook 7H9) with or without 0.2% glycerol and Hyg (100 μg/ml) when required.

    Techniques: In Vitro, Quantitation Assay

    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on Middlebrook 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .

    Journal: Frontiers in Microbiology

    Article Title: Functional Studies of Five Toxin-Antitoxin Modules in Mycobacterium tuberculosis H37Rv

    doi: 10.3389/fmicb.2016.02071

    Figure Lengend Snippet: Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on Middlebrook 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .

    Article Snippet: M. smegmatis mc2 155 was grown in Middlebrook 7H9 (a liquid medium, Difco) containing 0.2% glycerol and 0.02% Tween 80 or Middlebrook 7H10 (a solid agar medium, Difco) containing 0.2% glycerol.

    Techniques: Transformation Assay, Plasmid Preparation, Incubation