lipofectamine rnaimax Search Results


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  • 99
    Thermo Fisher lipofectamine rnaimax
    Photo-controlled IL1β protein silencing with mPEG- b -P(APNBMA) polyplexes, <t>Lipofectamine</t> <t>RNAiMAX</t> lipoplexes, and hybrid nanocomplexes. AoAFs were treated with siRNA using the various carriers, irradiated with 365 nm light for either 0 min (black bars) or 10 min (gold bars), and lysed for western blot analysis at 48 h post-transfection. Data represent the IL1β protein expression levels relative to the levels of the loading control glyceraldehyde 3-phosphate dehydrogenase (GAPDH), normalized to the protein levels in controls with no siRNA treatment. Results are shown as the mean ± standard deviation of data obtained from three independent experiments.
    Lipofectamine Rnaimax, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 51145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine rnaimax/product/Thermo Fisher
    Average 99 stars, based on 51145 article reviews
    Price from $9.99 to $1999.99
    lipofectamine rnaimax - by Bioz Stars, 2020-08
    99/100 stars
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    90
    Fisher Scientific lipofectamine rnaimax
    Photo-controlled IL1β protein silencing with mPEG- b -P(APNBMA) polyplexes, <t>Lipofectamine</t> <t>RNAiMAX</t> lipoplexes, and hybrid nanocomplexes. AoAFs were treated with siRNA using the various carriers, irradiated with 365 nm light for either 0 min (black bars) or 10 min (gold bars), and lysed for western blot analysis at 48 h post-transfection. Data represent the IL1β protein expression levels relative to the levels of the loading control glyceraldehyde 3-phosphate dehydrogenase (GAPDH), normalized to the protein levels in controls with no siRNA treatment. Results are shown as the mean ± standard deviation of data obtained from three independent experiments.
    Lipofectamine Rnaimax, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine rnaimax/product/Fisher Scientific
    Average 90 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    lipofectamine rnaimax - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    92
    Horizon Discovery lipofectamine rnaimax
    Photo-controlled IL1β protein silencing with mPEG- b -P(APNBMA) polyplexes, <t>Lipofectamine</t> <t>RNAiMAX</t> lipoplexes, and hybrid nanocomplexes. AoAFs were treated with siRNA using the various carriers, irradiated with 365 nm light for either 0 min (black bars) or 10 min (gold bars), and lysed for western blot analysis at 48 h post-transfection. Data represent the IL1β protein expression levels relative to the levels of the loading control glyceraldehyde 3-phosphate dehydrogenase (GAPDH), normalized to the protein levels in controls with no siRNA treatment. Results are shown as the mean ± standard deviation of data obtained from three independent experiments.
    Lipofectamine Rnaimax, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 92/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine rnaimax/product/Horizon Discovery
    Average 92 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    lipofectamine rnaimax - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    91
    Millipore lipofectamine rnaimax
    Photo-controlled IL1β protein silencing with mPEG- b -P(APNBMA) polyplexes, <t>Lipofectamine</t> <t>RNAiMAX</t> lipoplexes, and hybrid nanocomplexes. AoAFs were treated with siRNA using the various carriers, irradiated with 365 nm light for either 0 min (black bars) or 10 min (gold bars), and lysed for western blot analysis at 48 h post-transfection. Data represent the IL1β protein expression levels relative to the levels of the loading control glyceraldehyde 3-phosphate dehydrogenase (GAPDH), normalized to the protein levels in controls with no siRNA treatment. Results are shown as the mean ± standard deviation of data obtained from three independent experiments.
    Lipofectamine Rnaimax, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine rnaimax/product/Millipore
    Average 91 stars, based on 70 article reviews
    Price from $9.99 to $1999.99
    lipofectamine rnaimax - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    Image Search Results


    Photo-controlled IL1β protein silencing with mPEG- b -P(APNBMA) polyplexes, Lipofectamine RNAiMAX lipoplexes, and hybrid nanocomplexes. AoAFs were treated with siRNA using the various carriers, irradiated with 365 nm light for either 0 min (black bars) or 10 min (gold bars), and lysed for western blot analysis at 48 h post-transfection. Data represent the IL1β protein expression levels relative to the levels of the loading control glyceraldehyde 3-phosphate dehydrogenase (GAPDH), normalized to the protein levels in controls with no siRNA treatment. Results are shown as the mean ± standard deviation of data obtained from three independent experiments.

    Journal: Advanced biosystems

    Article Title: Attenuation of Maladaptive Responses in Aortic Adventitial Fibroblasts through Stimuli-Triggered siRNA Release from Lipid-Polymer Nanocomplexes

    doi: 10.1002/adbi.201700099

    Figure Lengend Snippet: Photo-controlled IL1β protein silencing with mPEG- b -P(APNBMA) polyplexes, Lipofectamine RNAiMAX lipoplexes, and hybrid nanocomplexes. AoAFs were treated with siRNA using the various carriers, irradiated with 365 nm light for either 0 min (black bars) or 10 min (gold bars), and lysed for western blot analysis at 48 h post-transfection. Data represent the IL1β protein expression levels relative to the levels of the loading control glyceraldehyde 3-phosphate dehydrogenase (GAPDH), normalized to the protein levels in controls with no siRNA treatment. Results are shown as the mean ± standard deviation of data obtained from three independent experiments.

    Article Snippet: Solutions of siRNA and Lipofectamine RNAiMAX were prepared in Opti-MEM and mixed according to Life Technologies’ protocol (to produce a final solution containing 0.2 µg siRNA and 3 µL Lipofectamine in a total volume of 96 µL).

    Techniques: Irradiation, Western Blot, Transfection, Expressing, Standard Deviation

    Occludin knockdown attenuates serine protease-induced increase in TER. A : SCBN cells were treated with 50, 100, or 300 nM occludin siRNA for 48 h and knockdown assessed by Western blotting. B and C : representative tracings of SCBN cells transfected with 300 nM occludin siRNA for 48 h, and treated with trypsin ( B , n = 6–9) or matriptase ( C , n = 4). Controls include untransfected cells, and RNAiMAX Lipofectamine with or without scrambled siRNA. Baseline TER ( n = 10–14) ( D ) and change in TER 15 min post serine protease treatment ( E and F ) were assessed. Occludin siRNA induced a significant reduction in baseline TER and attenuated the trypsin- and matriptase-induced increase in TER. * P

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: The serine protease-mediated increase in intestinal epithelial barrier function is dependent on occludin and requires an intact tight junction

    doi: 10.1152/ajpgi.00441.2015

    Figure Lengend Snippet: Occludin knockdown attenuates serine protease-induced increase in TER. A : SCBN cells were treated with 50, 100, or 300 nM occludin siRNA for 48 h and knockdown assessed by Western blotting. B and C : representative tracings of SCBN cells transfected with 300 nM occludin siRNA for 48 h, and treated with trypsin ( B , n = 6–9) or matriptase ( C , n = 4). Controls include untransfected cells, and RNAiMAX Lipofectamine with or without scrambled siRNA. Baseline TER ( n = 10–14) ( D ) and change in TER 15 min post serine protease treatment ( E and F ) were assessed. Occludin siRNA induced a significant reduction in baseline TER and attenuated the trypsin- and matriptase-induced increase in TER. * P

    Article Snippet: Cells were transfected using RNAiMAX Lipofectamine (Invitrogen) in media without antibiotics.

    Techniques: Western Blot, Transfection

    Intracellular amount of emodin in K562/ADM and K562 cells. Both cells were incubated with various concentrations of emodin (0.1–10 μM) at 37°C or 4°C for 2 h (A); K562/ADM cells were co-treated with 0, 1, 3, 10 μM of verapamil or 0, 2, 4, 10 μM of cyclosporine A and emodin (5 μM) for 2 h (B). RNAi of P-gp (C). A-: K562/ADM cells transfected with negative control siRNA; AL: K562/ADM cells transfected with Lipofectamine RNAiMAX Reagent; AP: K562/ADM cells transfected with P-gp siRNA; A: K562/ADM cells; S: K562 cells. After transfection with P-gp siRNA, intracellular amount of emodin (5 μM) was enhanced compared with other control groups (D). Data were represented as the mean ± S.D. of three independent experiments. * P

    Journal: PLoS ONE

    Article Title: Emodin reverses leukemia multidrug resistance by competitive inhibition and downregulation of P-glycoprotein

    doi: 10.1371/journal.pone.0187971

    Figure Lengend Snippet: Intracellular amount of emodin in K562/ADM and K562 cells. Both cells were incubated with various concentrations of emodin (0.1–10 μM) at 37°C or 4°C for 2 h (A); K562/ADM cells were co-treated with 0, 1, 3, 10 μM of verapamil or 0, 2, 4, 10 μM of cyclosporine A and emodin (5 μM) for 2 h (B). RNAi of P-gp (C). A-: K562/ADM cells transfected with negative control siRNA; AL: K562/ADM cells transfected with Lipofectamine RNAiMAX Reagent; AP: K562/ADM cells transfected with P-gp siRNA; A: K562/ADM cells; S: K562 cells. After transfection with P-gp siRNA, intracellular amount of emodin (5 μM) was enhanced compared with other control groups (D). Data were represented as the mean ± S.D. of three independent experiments. * P

    Article Snippet: The Lipofectamine RNAiMAX Reagent was purchased from Invitrogen Trading Co., Ltd (Shanghai, China).

    Techniques: Incubation, Transfection, Negative Control

    In vitro function of siRNA-TLP and cell viability. ( a ) Western blot time course of siRNA-TLP function regulating AR expression in LNCaP cells. ( b ) Cell viability of LNCaP cells after treatment with siRNA-TLPs measured by MTS assay (1, 5, 10, 20 nM siRNA-TLP). ( c ) LNCaP cell confluence measured over time after treatment with siRNA-TLPs (20 nM), and images taken 165 hours after siRNA-TLP treatment depicting cell confluence. AR = siRNA targeting the androgen receptor. Ctrl = scrambled control siRNA sequence. Lipo = Lipofectamine ® RNAiMax.

    Journal: Advanced functional materials

    Article Title: Properties of Native High-Density Lipoproteins Inspire Synthesis of Actively Targeted In Vivo siRNA Delivery Vehicles

    doi: 10.1002/adfm.201602600

    Figure Lengend Snippet: In vitro function of siRNA-TLP and cell viability. ( a ) Western blot time course of siRNA-TLP function regulating AR expression in LNCaP cells. ( b ) Cell viability of LNCaP cells after treatment with siRNA-TLPs measured by MTS assay (1, 5, 10, 20 nM siRNA-TLP). ( c ) LNCaP cell confluence measured over time after treatment with siRNA-TLPs (20 nM), and images taken 165 hours after siRNA-TLP treatment depicting cell confluence. AR = siRNA targeting the androgen receptor. Ctrl = scrambled control siRNA sequence. Lipo = Lipofectamine ® RNAiMax.

    Article Snippet: For comparisons against conventional transfection reagents, Lipofectamine® RNAiMax transfections were used to treat cells with Ctrl, AR, EZH2, or SR-B1 siRNAs according to the protocol provided by the manufacturer (Invitrogen).

    Techniques: In Vitro, Western Blot, Expressing, MTS Assay, Sequencing

    Stability of RNA on siRNA-TLP and siRNA-TLP function after incubation in human serum. ( a ) RNA stability of siRNA-TLPs compared to free RNA sequences upon exposure to RNase A. ( b ) RNA stability of siRNA-TLPs compared to free RNA sequences after exposure to human plasma. ( c ) Western blot of AR protein expression after treatment of LNCaP cells with TLPs and siRNA-TLPs that have been incubated in human serum (48 hrs, 20 nM siRNA-TLP), and serum supernatant (SN) fraction containing only albumin and HDL separated from siRNA-TLPs. The particle type added to human serum is indicated in parenthesis. AR = siRNA targeting the androgen receptor. Ctrl = scrambled control siRNA sequence. Lipo = Lipofectamine ® RNAiMax.

    Journal: Advanced functional materials

    Article Title: Properties of Native High-Density Lipoproteins Inspire Synthesis of Actively Targeted In Vivo siRNA Delivery Vehicles

    doi: 10.1002/adfm.201602600

    Figure Lengend Snippet: Stability of RNA on siRNA-TLP and siRNA-TLP function after incubation in human serum. ( a ) RNA stability of siRNA-TLPs compared to free RNA sequences upon exposure to RNase A. ( b ) RNA stability of siRNA-TLPs compared to free RNA sequences after exposure to human plasma. ( c ) Western blot of AR protein expression after treatment of LNCaP cells with TLPs and siRNA-TLPs that have been incubated in human serum (48 hrs, 20 nM siRNA-TLP), and serum supernatant (SN) fraction containing only albumin and HDL separated from siRNA-TLPs. The particle type added to human serum is indicated in parenthesis. AR = siRNA targeting the androgen receptor. Ctrl = scrambled control siRNA sequence. Lipo = Lipofectamine ® RNAiMax.

    Article Snippet: For comparisons against conventional transfection reagents, Lipofectamine® RNAiMax transfections were used to treat cells with Ctrl, AR, EZH2, or SR-B1 siRNAs according to the protocol provided by the manufacturer (Invitrogen).

    Techniques: Incubation, Western Blot, Expressing, Sequencing

    Cell uptake of siRNA-TLP and functional dependence on scavenger receptor type B-1 (SR-B1). ( a ) Cellular uptake of siRNA-TLP by LNCaP cells measured by fluorescent signal in cells over time using an Incucyte ZOOM (20 nM siRNA-TLP). ( b ) Representative fluorescent images of siRNA-TLP uptake (72 hrs). ( c ) Western blot of LNCaP cells pre-treated with SR-B1 and Ctrl siRNA 48 hrs prior to siRNA-TLPs addition to determine if function is dependent on cellular SR-B1 expression. The hours in parenthesis indicate the total treatment time of siRNA sequences added using Lipofectamine ® RNAiMAX. LNCaP cells were treated with siRNA-TLPs for 48 hrs. Quantification of western blot data. AR = siRNA targeting the androgen receptor. Ctrl = scrambled control siRNA sequence. Lipo = Lipofectamine ® RNAiMax.

    Journal: Advanced functional materials

    Article Title: Properties of Native High-Density Lipoproteins Inspire Synthesis of Actively Targeted In Vivo siRNA Delivery Vehicles

    doi: 10.1002/adfm.201602600

    Figure Lengend Snippet: Cell uptake of siRNA-TLP and functional dependence on scavenger receptor type B-1 (SR-B1). ( a ) Cellular uptake of siRNA-TLP by LNCaP cells measured by fluorescent signal in cells over time using an Incucyte ZOOM (20 nM siRNA-TLP). ( b ) Representative fluorescent images of siRNA-TLP uptake (72 hrs). ( c ) Western blot of LNCaP cells pre-treated with SR-B1 and Ctrl siRNA 48 hrs prior to siRNA-TLPs addition to determine if function is dependent on cellular SR-B1 expression. The hours in parenthesis indicate the total treatment time of siRNA sequences added using Lipofectamine ® RNAiMAX. LNCaP cells were treated with siRNA-TLPs for 48 hrs. Quantification of western blot data. AR = siRNA targeting the androgen receptor. Ctrl = scrambled control siRNA sequence. Lipo = Lipofectamine ® RNAiMax.

    Article Snippet: For comparisons against conventional transfection reagents, Lipofectamine® RNAiMax transfections were used to treat cells with Ctrl, AR, EZH2, or SR-B1 siRNAs according to the protocol provided by the manufacturer (Invitrogen).

    Techniques: Functional Assay, Western Blot, Expressing, Sequencing

    siRNA-TLP optimization, and function. ( a ) UV-Vis spectroscopy measurement of nanoparticle (λ max ~520 nm) and RNA (λ max ~260 nm) after centrifugation for purification from unreacted components. ( b ) ζ-potential measurement of TLPs, DOTAP-TLPs and siRNA-TLP particles synthesized with increasing DOTAP:RNA molar ratios from 10:1–40:1. ( c ) Hydrodynamic diameter (nm) measurement of 5 nm Au NP (PDI: 0.313 ± 0.192), TLP (PDI: 0.478 ± 0.140), and siRNA-TLP particles synthesized with increasing DOTAP:RNA molar ratios from 10:1–40:1. The polydispersity indices (PDI) are as follows: AR-TLP 10:1 (PDI: 0.184 ± 0.011), Ctrl-TLP 10:1 (PDI: 0.190 ± 0.003), AR-TLP 20:1 (PDI: 0.153 ± 0.004), Ctrl-TLP 20:1 (PDI: 0.183 ± 0.003), AR-TLP 30:1 (PDI: 0.174 ± 0.012), Ctrl-TLP 30:1 (PDI: 0.229 ± 0.007), AR-TLP 40:1 (0.118 ± 0.016), Ctrl-TLP 40:1 (0.121 ± 0.008). ( d ) UV-Vis spectroscopy measuring purified siRNA-TLPs synthesized with increasing DOTAP:RNA molar ratios. ( e ) Western blot of siRNA-TLP function regulating target AR expression in LNCaP cells (48 hrs, 20 nM siRNA-TLP). All UV-Vis spectra were normalized according to λ max ~520 nm. AR = siRNA targeting the androgen receptor. Ctrl = scrambled control siRNA sequence. Lipo = Lipofectamine ® RNAiMax.

    Journal: Advanced functional materials

    Article Title: Properties of Native High-Density Lipoproteins Inspire Synthesis of Actively Targeted In Vivo siRNA Delivery Vehicles

    doi: 10.1002/adfm.201602600

    Figure Lengend Snippet: siRNA-TLP optimization, and function. ( a ) UV-Vis spectroscopy measurement of nanoparticle (λ max ~520 nm) and RNA (λ max ~260 nm) after centrifugation for purification from unreacted components. ( b ) ζ-potential measurement of TLPs, DOTAP-TLPs and siRNA-TLP particles synthesized with increasing DOTAP:RNA molar ratios from 10:1–40:1. ( c ) Hydrodynamic diameter (nm) measurement of 5 nm Au NP (PDI: 0.313 ± 0.192), TLP (PDI: 0.478 ± 0.140), and siRNA-TLP particles synthesized with increasing DOTAP:RNA molar ratios from 10:1–40:1. The polydispersity indices (PDI) are as follows: AR-TLP 10:1 (PDI: 0.184 ± 0.011), Ctrl-TLP 10:1 (PDI: 0.190 ± 0.003), AR-TLP 20:1 (PDI: 0.153 ± 0.004), Ctrl-TLP 20:1 (PDI: 0.183 ± 0.003), AR-TLP 30:1 (PDI: 0.174 ± 0.012), Ctrl-TLP 30:1 (PDI: 0.229 ± 0.007), AR-TLP 40:1 (0.118 ± 0.016), Ctrl-TLP 40:1 (0.121 ± 0.008). ( d ) UV-Vis spectroscopy measuring purified siRNA-TLPs synthesized with increasing DOTAP:RNA molar ratios. ( e ) Western blot of siRNA-TLP function regulating target AR expression in LNCaP cells (48 hrs, 20 nM siRNA-TLP). All UV-Vis spectra were normalized according to λ max ~520 nm. AR = siRNA targeting the androgen receptor. Ctrl = scrambled control siRNA sequence. Lipo = Lipofectamine ® RNAiMax.

    Article Snippet: For comparisons against conventional transfection reagents, Lipofectamine® RNAiMax transfections were used to treat cells with Ctrl, AR, EZH2, or SR-B1 siRNAs according to the protocol provided by the manufacturer (Invitrogen).

    Techniques: UV-Vis Spectroscopy, Centrifugation, Purification, Synthesized, Western Blot, Expressing, Sequencing