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  • 99
    Thermo Fisher lipofectamine 3000 kits
    PD-L1 disruption assay of Cas9/sgRNA and siRNA delivered by Lipofectamine 3000. Abbreviation:  PD-L1, programmed cell death ligand-1.
    Lipofectamine 3000 Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 107 article reviews
    Price from $9.99 to $1999.99
    lipofectamine 3000 kits - by Bioz Stars, 2020-08
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    91
    Fisher Scientific lipofectamine 3000
    PD-L1 disruption assay of Cas9/sgRNA and siRNA delivered by Lipofectamine 3000. Abbreviation:  PD-L1, programmed cell death ligand-1.
    Lipofectamine 3000, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 91/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 3000/product/Fisher Scientific
    Average 91 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    lipofectamine 3000 - by Bioz Stars, 2020-08
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    93
    Roche lipofectamine 3000
    PD-L1 disruption assay of Cas9/sgRNA and siRNA delivered by Lipofectamine 3000. Abbreviation:  PD-L1, programmed cell death ligand-1.
    Lipofectamine 3000, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 3000/product/Roche
    Average 93 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    lipofectamine 3000 - by Bioz Stars, 2020-08
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    90
    promega lipofectamine 3000
    PD-L1 disruption assay of Cas9/sgRNA and siRNA delivered by Lipofectamine 3000. Abbreviation:  PD-L1, programmed cell death ligand-1.
    Lipofectamine 3000, supplied by promega, used in various techniques. Bioz Stars score: 90/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 3000/product/promega
    Average 90 stars, based on 162 article reviews
    Price from $9.99 to $1999.99
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    91
    Millipore lipofectamine 3000
    PD-L1 disruption assay of Cas9/sgRNA and siRNA delivered by Lipofectamine 3000. Abbreviation:  PD-L1, programmed cell death ligand-1.
    Lipofectamine 3000, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 3000/product/Millipore
    Average 91 stars, based on 109 article reviews
    Price from $9.99 to $1999.99
    lipofectamine 3000 - by Bioz Stars, 2020-08
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    92
    GE Healthcare lipofectamine 3000
    PD-L1 disruption assay of Cas9/sgRNA and siRNA delivered by Lipofectamine 3000. Abbreviation:  PD-L1, programmed cell death ligand-1.
    Lipofectamine 3000, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 3000/product/GE Healthcare
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    lipofectamine 3000 - by Bioz Stars, 2020-08
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    Fisher Scientific lipofectamine 3000 reagent
    PD-L1 disruption assay of Cas9/sgRNA and siRNA delivered by Lipofectamine 3000. Abbreviation:  PD-L1, programmed cell death ligand-1.
    Lipofectamine 3000 Reagent, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 3000 reagent/product/Fisher Scientific
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    lipofectamine 3000 reagent - by Bioz Stars, 2020-08
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    origene lipofectamine 3000
    PD-L1 disruption assay of Cas9/sgRNA and siRNA delivered by Lipofectamine 3000. Abbreviation:  PD-L1, programmed cell death ligand-1.
    Lipofectamine 3000, supplied by origene, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 3000/product/origene
    Average 92 stars, based on 23 article reviews
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    genepharma company lipofectamine 3000
    PD-L1 disruption assay of Cas9/sgRNA and siRNA delivered by Lipofectamine 3000. Abbreviation:  PD-L1, programmed cell death ligand-1.
    Lipofectamine 3000, supplied by genepharma company, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 3000/product/genepharma company
    Average 92 stars, based on 32 article reviews
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    lipofectamine 3000 - by Bioz Stars, 2020-08
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    Thermo Fisher lipofactamine 3000
    PD-L1 disruption assay of Cas9/sgRNA and siRNA delivered by Lipofectamine 3000. Abbreviation:  PD-L1, programmed cell death ligand-1.
    Lipofactamine 3000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofactamine 3000/product/Thermo Fisher
    Average 90 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
    lipofactamine 3000 - by Bioz Stars, 2020-08
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    93
    Promega lipofectamine 3000 reagent
    PD-L1 disruption assay of Cas9/sgRNA and siRNA delivered by Lipofectamine 3000. Abbreviation:  PD-L1, programmed cell death ligand-1.
    Lipofectamine 3000 Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 3000 reagent/product/Promega
    Average 93 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    lipofectamine 3000 reagent - by Bioz Stars, 2020-08
    93/100 stars
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    91
    Millipore lipofectamine 3000 reagent
    PD-L1 disruption assay of Cas9/sgRNA and siRNA delivered by Lipofectamine 3000. Abbreviation:  PD-L1, programmed cell death ligand-1.
    Lipofectamine 3000 Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 3000 reagent/product/Millipore
    Average 91 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    lipofectamine 3000 reagent - by Bioz Stars, 2020-08
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    Image Search Results


    PD-L1 disruption assay of Cas9/sgRNA and siRNA delivered by Lipofectamine 3000. Abbreviation:  PD-L1, programmed cell death ligand-1.

    Journal: International Journal of Nanomedicine

    Article Title: Stearyl polyethylenimine complexed with plasmids as the core of human serum albumin nanoparticles noncovalently bound to CRISPR/Cas9 plasmids or siRNA for disrupting or silencing PD-L1 expression for immunotherapy

    doi: 10.2147/IJN.S181440

    Figure Lengend Snippet: PD-L1 disruption assay of Cas9/sgRNA and siRNA delivered by Lipofectamine 3000. Abbreviation: PD-L1, programmed cell death ligand-1.

    Article Snippet: Lipofectamine 3000, Hoechst 33342, FBS, and mouse interferon (IFN)-γ recombinant protein were provided by Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques:

    siRNA-mediated silencing of PCGF isoforms increases  E. chaffeensis  infection. THP-1 cells were transfected with isoform-specific siRNA and then infected with  E. chaffeensis  at 24 h posttransfection. (A) Alexa Fluor 488-conjugated siRNA-transfected cell, showing high efficiency of RNA transfection using Lipofectamine 3000. (B) Western blot analysis of the total cell lysate from control and siRNA-transfected THP-1 cells confirmed the decrease in PCGF2, PCGF3, PCGF4, and PCGF5 48 h posttransfection. GAPDH was used as the loading control. The relative abundance of PCGF isoforms in siRNA-transfected cells was determined after normalization to the loading control and then represented as the percentage remaining after the knockdown. (C) Table representing the percentage increase in ehrlichial morulae and the average number of morulae/cell for each PCGF isoform-specific knockdown. The average morula counts were determined by counting the number of morula present in each field of view and then dividing that by the number of cells counted. The experiment was repeated three times in duplicate, and the values shown are means ± standard deviations (Stdev). (D) The fold change in ehrlichial infection was determined by comparing the ehrlichial  dsb  to the host cell  gapdh  in individual PCGF knockdown using real-time qPCR at 48 hpi ( n  = 3; *,  P  ≤ 0.05).

    Journal: Infection and Immunity

    Article Title: Ehrlichia chaffeensis TRP120 Effector Targets and Recruits Host Polycomb Group Proteins for Degradation To Promote Intracellular Infection

    doi: 10.1128/IAI.00845-17

    Figure Lengend Snippet: siRNA-mediated silencing of PCGF isoforms increases E. chaffeensis infection. THP-1 cells were transfected with isoform-specific siRNA and then infected with E. chaffeensis at 24 h posttransfection. (A) Alexa Fluor 488-conjugated siRNA-transfected cell, showing high efficiency of RNA transfection using Lipofectamine 3000. (B) Western blot analysis of the total cell lysate from control and siRNA-transfected THP-1 cells confirmed the decrease in PCGF2, PCGF3, PCGF4, and PCGF5 48 h posttransfection. GAPDH was used as the loading control. The relative abundance of PCGF isoforms in siRNA-transfected cells was determined after normalization to the loading control and then represented as the percentage remaining after the knockdown. (C) Table representing the percentage increase in ehrlichial morulae and the average number of morulae/cell for each PCGF isoform-specific knockdown. The average morula counts were determined by counting the number of morula present in each field of view and then dividing that by the number of cells counted. The experiment was repeated three times in duplicate, and the values shown are means ± standard deviations (Stdev). (D) The fold change in ehrlichial infection was determined by comparing the ehrlichial dsb to the host cell gapdh in individual PCGF knockdown using real-time qPCR at 48 hpi ( n = 3; *, P ≤ 0.05).

    Article Snippet: Briefly, specific siRNA (3 μl) and Lipofectamine 3000 reagent (7.5 μl) were added to Opti-MEM medium (250 μl) (Invitrogen), incubated for 5 min at room temperature, and then added to the cell suspension in a 6-well plate.

    Techniques: Infection, Transfection, Western Blot, Real-time Polymerase Chain Reaction

    Knockout of p47phox prevents sinus nodal cell death and CaMKII oxidization observed with Ang II. Cas9 KO plasmid transfection of p47phox in sinus nodal cell was performed using a Lipofectamine 3000. Plated cells were exposed to the Cas9 KO plasmid or empty vector control. After this, Ang II (20 µM) was added to the culture medium for 5 days. From these cells, ox-CaMKII levels (A), apoptotic cells (B), and caspase-3 activity (C) were evaluated. Ang II, angiotensin II; CaMKII, calmodulin kinase II; ox-CaMKII, oxidized-CaMKII; PBS, phosphate buffered saline; T-CaMKII, total CaMKII; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling. Data are shown as the means±SEM of 3-6 mice per group.  a) Significantly different ( P

    Journal: Anatomy & Cell Biology

    Article Title: Protective effects of kaempferol against cardiac sinus node dysfunction via CaMKII deoxidization

    doi: 10.5115/acb.2015.48.4.235

    Figure Lengend Snippet: Knockout of p47phox prevents sinus nodal cell death and CaMKII oxidization observed with Ang II. Cas9 KO plasmid transfection of p47phox in sinus nodal cell was performed using a Lipofectamine 3000. Plated cells were exposed to the Cas9 KO plasmid or empty vector control. After this, Ang II (20 µM) was added to the culture medium for 5 days. From these cells, ox-CaMKII levels (A), apoptotic cells (B), and caspase-3 activity (C) were evaluated. Ang II, angiotensin II; CaMKII, calmodulin kinase II; ox-CaMKII, oxidized-CaMKII; PBS, phosphate buffered saline; T-CaMKII, total CaMKII; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling. Data are shown as the means±SEM of 3-6 mice per group. a) Significantly different ( P

    Article Snippet: Knockout of p47phox by Cas9 KO plasmid Cas9 KO plasmid transfection of p47phox in sinus nodal cell was performed using a Lipofectamine 3000 Kit (Life Technologies, CA, USA).

    Techniques: Knock-Out, Plasmid Preparation, Transfection, Activity Assay, TUNEL Assay, Mouse Assay

    ERK1/2 knockdown suppresses PDCoV infection. (A) ST cells were transfected with 100 nM ERK-specific siRNA or control siRNA using Lipofectamine 3000 followed by PDCoV infection at 24 h post-transfection. At 12 hpi, cellular lysates were prepared and subjected to immunoblotting using an antibody against ERK1/2, p-ERK1/2, or p-Elk-1. The blot was also reacted with an anti-β-actin antibody to verify equal protein leading. Each protein expression was quantitatively analyzed by densitometry and presented as the relative density value to the β-actin gene, and fold changes in each protein:β-actin ratio are plotted. (B) For immunostaining, transfected and infected cells were fixed at 12 hpi and stained with an anti-PDCoV N antibody followed by Alexa green-conjugated goat anti-mouse secondary antibody (first panels). The cells were then counterstained with DAPI (second panels) and examined under a fluorescent microscope at 200× magnification. Quantification of PDCoV production was assessed exactly as described in the legend of Fig. 4 B and presented (left panel) (C) The virus supernatants were collected at 12 hpi, and viral titers were determined. Values are representative of the mean of three independent experiments, and error bars represent the mean ± SDM. ** P

    Journal: Virus Research

    Article Title: Porcine deltacoronavirus activates the Raf/MEK/ERK pathway to promote its replication

    doi: 10.1016/j.virusres.2020.197961

    Figure Lengend Snippet: ERK1/2 knockdown suppresses PDCoV infection. (A) ST cells were transfected with 100 nM ERK-specific siRNA or control siRNA using Lipofectamine 3000 followed by PDCoV infection at 24 h post-transfection. At 12 hpi, cellular lysates were prepared and subjected to immunoblotting using an antibody against ERK1/2, p-ERK1/2, or p-Elk-1. The blot was also reacted with an anti-β-actin antibody to verify equal protein leading. Each protein expression was quantitatively analyzed by densitometry and presented as the relative density value to the β-actin gene, and fold changes in each protein:β-actin ratio are plotted. (B) For immunostaining, transfected and infected cells were fixed at 12 hpi and stained with an anti-PDCoV N antibody followed by Alexa green-conjugated goat anti-mouse secondary antibody (first panels). The cells were then counterstained with DAPI (second panels) and examined under a fluorescent microscope at 200× magnification. Quantification of PDCoV production was assessed exactly as described in the legend of Fig. 4 B and presented (left panel) (C) The virus supernatants were collected at 12 hpi, and viral titers were determined. Values are representative of the mean of three independent experiments, and error bars represent the mean ± SDM. ** P

    Article Snippet: 2.8 Knocked down expression of ERK by siRNA ST cells were transfected with 100 nM siRNA targeting ERK1/2 or control siRNA using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocols.

    Techniques: Infection, Transfection, Expressing, Immunostaining, Staining, Microscopy