Journal: American Journal of Nuclear Medicine and Molecular Imaging

Article Title: Core-based lipid nanoparticles as a nanoplatform for delivery of near-infrared fluorescent imaging agents

doi:

Figure Lengend Snippet: Schematic structure of LNP. PEGylated lipids regulate the solubility of LNPs in water solutions. Metal chelating groups are used for binding protein via a His6 tag. The His6 tag controls the protein ligand binding site orientation allowing the attachment of protein molecules without any chemical modification. The Pyro molecules are conjugated to cholesterol or to glycerol based lipids. Fluorescent properties of the molecule will allow in vivo molecular imaging and photodynamic therapy. The lipid core composition has influence on the stability of the LNPs.

Article Snippet: To study how the nature of the core influences LNP stability and size, we designed and assembled LNPs with different core lipids: (i) cholesterol (CE); (ii) cholesterol oleate (CE-OA); (iii) N-BOC-protected amino cholesterol oleate (BOC-CE-OA).

Techniques: Solubility, Binding Assay, Ligand Binding Assay, Modification, In Vivo, Imaging

Journal: American Journal of Nuclear Medicine and Molecular Imaging

Article Title: Core-based lipid nanoparticles as a nanoplatform for delivery of near-infrared fluorescent imaging agents

doi:

Figure Lengend Snippet: Size distribution of the LNPs (A) with Boc modified cholesterol oleate (BOC-CE-OA) core, (B) without core, (C) with cholesterol oleate core, and (D) with cholesterol core as measured by dynamic light scattering. The structure of corresponding LNP core components are shown on the right side of the Figure.

Article Snippet: To study how the nature of the core influences LNP stability and size, we designed and assembled LNPs with different core lipids: (i) cholesterol (CE); (ii) cholesterol oleate (CE-OA); (iii) N-BOC-protected amino cholesterol oleate (BOC-CE-OA).

Techniques: Modification

Journal: American Journal of Nuclear Medicine and Molecular Imaging

Article Title: Core-based lipid nanoparticles as a nanoplatform for delivery of near-infrared fluorescent imaging agents

doi:

Figure Lengend Snippet: Binding of ICAM-1-LNP-Pyro-PtdEtn to CD8+ CER43 T cells expressing cell surface αLβ2 integrins that recognize ICAM-1. The cells were incubated with the LNPs conjugate in the presence (A) or absence (B) of Ca2+ and Mg2+ in the staining buffer. Cells incubated with plain LNPs, i.e., untargeted nanoparticles (grey) were used as negative control. The mean fluorescent intensity of the cells was measured by flow cytometry. Fluorescence was excited at 633 nm and emission collected at 675 nm. (C) The dependence of ICAM-1-NP-Pyro-PtdEtn binding to CER-43 cells on ICAM-1 concentration is shown. Cells incubated with untargeted nanoparticles were utilized as a negative control. Mean±SD is shown, (*)=p<0.05.

Article Snippet: To study how the nature of the core influences LNP stability and size, we designed and assembled LNPs with different core lipids: (i) cholesterol (CE); (ii) cholesterol oleate (CE-OA); (iii) N-BOC-protected amino cholesterol oleate (BOC-CE-OA).

Techniques: Binding Assay, Expressing, Incubation, Staining, Negative Control, Flow Cytometry, Fluorescence, Concentration Assay

Journal: American Journal of Nuclear Medicine and Molecular Imaging

Article Title: Core-based lipid nanoparticles as a nanoplatform for delivery of near-infrared fluorescent imaging agents

doi:

Figure Lengend Snippet: Binding of (A) ICAM-1-LNP-Pyro-PtdEtn and (B) ICAM-1-LNP-Pyro-CE-OA to CD8+ CER43 T cells. Binding of the LNPs loaded with irrelevant pHLA-DRB1 protein is shown with grey line. Cells incubated with plain LNPs, i.e., untargeted nanoparticles (grey) were used as negative control. (C and D) Time course of ICAM-1-LNPs (black) binding to CD8+ CER43 T cells. CER-43 cells were incubated in the presence of (C) ICAM-1-LNP-Pyro-PtdEtn or (D) ICAM-1-LNP-Pyro-CE-OA for 30 min to 24 h. Cells incubated with untargeted LNPs (grey) were used as negative control. After exhaustive washing, the cells were analyzed by flow cytometry. Mean±SD is shown, (*)=p<0.05. The mean fluorescent intensity of the cells was measured by flow cytometry. Fluorescence was excited at 633 nm with emission at 675 nm.

Article Snippet: To study how the nature of the core influences LNP stability and size, we designed and assembled LNPs with different core lipids: (i) cholesterol (CE); (ii) cholesterol oleate (CE-OA); (iii) N-BOC-protected amino cholesterol oleate (BOC-CE-OA).

Techniques: Binding Assay, Incubation, Negative Control, Flow Cytometry, Fluorescence

Journal: American Journal of Nuclear Medicine and Molecular Imaging

Article Title: Core-based lipid nanoparticles as a nanoplatform for delivery of near-infrared fluorescent imaging agents

doi:

Figure Lengend Snippet: Fluorescent microscopy of CER-43 cells stained with (A) ICAM-1-LNP-Pyro- PtdEtn and (B) plain LNPs. CER-43 cells were stained with LNPs for 3 hr at 37°C. After washing the samples were used for microscopy on a wide field fluorescence microscope. The near-infrared fluorescence of the probes was excited by Xenon Lamp at 620/60 nm and emission was collected with 700/75 nm band pass filter.

Article Snippet: To study how the nature of the core influences LNP stability and size, we designed and assembled LNPs with different core lipids: (i) cholesterol (CE); (ii) cholesterol oleate (CE-OA); (iii) N-BOC-protected amino cholesterol oleate (BOC-CE-OA).

Techniques: Microscopy, Staining, Fluorescence

Journal: Nutrition Journal

Article Title: Effects of supplementing n-3 fatty acid enriched eggs and walnuts on cardiovascular disease risk markers in healthy free-living lacto-ovo-vegetarians: a randomized, crossover, free-living intervention study

doi: 10.1186/1475-2891-13-29

Figure Lengend Snippet: Fatty acid composition of erythrocyte membranes before and after each of the three dietary treatments

Article Snippet: Lipomics Technologies (West Sacramento, CA) extracted and analyzed the erythrocyte membrane lipids as described previously [ ].

Techniques: Control