Journal:

Article Title: Reverse Sanger sequencing of RNA by MALDI-TOF mass spectrometry after solid phase purification

doi: 10.1093/nar/gnh089

Figure Lengend Snippet: MALDI mass spectra of the RNA in vitro transcription performed with pGEM 3Zf linearized with (A) HindIII (sequence of 60mer: 5′-PPP-GGGCGAAUUCGAGCUCGGUACCCGGGGAUCCUCUAGAGUCGACCUGCAGGCAUGCAAGCU-3′) or with (B) SmaI (sequence of 23mer: 5′-PPP-GGGCGAAUUCGAGCUCGGUACCC-3′) and transcribed with T7 RNA polymerase utilizing unmodified NTP after EtOH-precipitation and ZipTip purification. The byproducts, abortive products (2–12 nt) and slippage products (G4–G7), are labeled according to their length.

Article Snippet: RNA was synthesized by runoff transcription from a linearized plasmid DNA vector pGEM 3Zf (Promega, Mannheim, Germany) carrying two promotors directed at each other from opposite ends of a multiple cloning site.

Techniques: In Vitro, Sequencing, Purification, Labeling

Journal:

Article Title: Reverse Sanger sequencing of RNA by MALDI-TOF mass spectrometry after solid phase purification

doi: 10.1093/nar/gnh089

Figure Lengend Snippet: MALDI mass spectra of RNA transcripts after solid-phase purification by hybridization using magnetic beads No. 2 (A) or magnetic beads No. 1 (B). In vitro transcription was performed with pGEM 3Zf linearized (A) with HindIII (sequence of 60mer: 5′-GGGCGAAUUCGAGCUCGGUACCCGGGGAUCCUCUAGAGUCGACCUGCAGGCAUGCAAGCU-3′). Signals marked with open circle: leakage bond of the universal complementary sequence coated to magnetic beads; (B) with SmaI (sequence of 23mer: 5′-PPP-GGGCGAAUUCGAGCUCGGUACCC-3′) and transcribed with T7 RNA polymerase utilizing unmodified NTP.

Article Snippet: RNA was synthesized by runoff transcription from a linearized plasmid DNA vector pGEM 3Zf (Promega, Mannheim, Germany) carrying two promotors directed at each other from opposite ends of a multiple cloning site.

Techniques: Purification, Hybridization, Magnetic Beads, In Vitro, Sequencing

Journal: Frontiers in Endocrinology

Article Title: Transcriptional Regulation of Vih by Oct4 and Sox9 in Scylla paramamosain

doi: 10.3389/fendo.2020.00650

Figure Lengend Snippet: Primers used in this study.

Article Snippet: These dsRNAs were synthesized from the linearized template by using the T7 RiboMAXTM Express RNAi System (Promega).

Techniques: Sequencing, Transfection, Expressing

Journal: Frontiers in Endocrinology

Article Title: Transcriptional Regulation of Vih by Oct4 and Sox9 in Scylla paramamosain

doi: 10.3389/fendo.2020.00650

Figure Lengend Snippet: Oct4 or Sox9 RNA interference reduces the expression level of SpVih . (A) Relative expression of Oct4 in the eyestalk after injection of dsOct4, dsEGFP, and SPSS. (B) Relative expression of Sox9 in the eyestalk after injection of dsOct4, dsEGFP, and SPSS. (C) The relative expression of SpVih in the eyestalk after injection of dsSox9, dsOct4, dsEGFP, and SPSS. NC, negative control group; BC, blank control group; Bar with different letters indicates significant differences, P < 0.05.

Article Snippet: These dsRNAs were synthesized from the linearized template by using the T7 RiboMAXTM Express RNAi System (Promega).

Techniques: Expressing, Injection, Negative Control, Control

Journal: Frontiers in Endocrinology

Article Title: Transcriptional Regulation of Vih by Oct4 and Sox9 in Scylla paramamosain

doi: 10.3389/fendo.2020.00650

Figure Lengend Snippet: Oct4 or Sox9 RNA interference promotes the expression level of SpVtg . (A) Relative expression of SpVtg in the ovary after injection of dsSox9, dsOct4, dsEGFP, and SPSS. (B) The relative expression of SpVtg in hepatopancreas after injection of dsSox9, dsOct4, dsEGFP, and SPSS. NC, negative control group; BC, blank control group; Bar with different letters indicates significant differences, P < 0.05.

Article Snippet: These dsRNAs were synthesized from the linearized template by using the T7 RiboMAXTM Express RNAi System (Promega).

Techniques: Expressing, Injection, Negative Control, Control