Journal: Asian-Australasian Journal of Animal Sciences

Article Title: LIMK1/2 are required for actin filament and cell junction assembly in porcine embryos developing in vitro

doi: 10.5713/ajas.19.0744

Figure Lengend Snippet: Decreasing of blastocyst rate by treatment of LIMKi3. (A and B) Effects of LIMKi3 during early embryo development. LIMKi3 was treated at the morula stage with different concentrations (0, 10, 50, 100, and 200 μM). The blastocyst formation rates reduced in adose-dependent manner. LIMK, LIM kinases; LIMKi3, LIMK inhibitor. Scale bars, 200 μm. * p<0.05, ** p<0.01.

Article Snippet: To inhibit LIMK1/2 during early embryo development, morula, diluted in dimethyl sulfoxide (DMSO) were treated with LIMKi3 (Tocris Bioscience, Minneapolis, MN, USA).

Techniques:

Journal: Asian-Australasian Journal of Animal Sciences

Article Title: LIMK1/2 are required for actin filament and cell junction assembly in porcine embryos developing in vitro

doi: 10.5713/ajas.19.0744

Figure Lengend Snippet: Effect of LIMK inhibition on embryos actin with cell junction. (A and B) Decreasing actin levels after LIMKi3 treatment at the morula stage. In the cell-to-cell boundaries, actin intensity was decreased in LIMKi3 treatment group compared with the control group. Blue, nucleus; red, actin. Scale bars, 50 μm. (C) Cell junction assembly breakdown in LIMKi3 treated embryos. After treatment of LIMKi3, AJ, and TJ proteins were decreased with actin and p-cofilin. Blue, nucleus; green, β-catenin, E-cadherin, ZO-1, CXADR or p-Cofilin; red, actin. (D) p-cofilin level after treatment of LIMKi3 in blastocyst stage. The intensity of p-cofilin decreased in LIMKi3 treated embryos. Intensity of p-cofilin was calculated using ImageJ software. LIMK, LIM kinases; LIMKi3, LIMK inhibitor. Scale bars, 50 μm.

Article Snippet: To inhibit LIMK1/2 during early embryo development, morula, diluted in dimethyl sulfoxide (DMSO) were treated with LIMKi3 (Tocris Bioscience, Minneapolis, MN, USA).

Techniques: Inhibition, Control, Software

Journal: International Journal of Neuropsychopharmacology

Article Title: LIMK1/2 in the mPFC Plays a Role in Chronic Stress-Induced Depressive-Like Effects in Mice

doi: 10.1093/ijnp/pyaa067

Figure Lengend Snippet: Infusion of LIMKi 3 into the medial prefrontal cortex (mPFC) produced antidepressant-like effects in the chronic unpredictable mild stress (CUMS) model of depression. (A) Schematic timeline of the experimental procedures. (B) LIMKi 3 fully prevented the CUMS-induced depressive-like behaviors in mice, as revealed by the forced swim test (FST), tail suspension test (TST), and sucrose preference test (n = 10). (C) Both the (CUMS + 100 μM LIMKi 3)-treated and (CUMS + 200 μM LIMKi 3)-treated mice had significantly lower levels of pLIMK1, pLIMK2, and pCofilin in the mPFC than the CUMS-treated mice (n = 6). The expression of total LIMK1, LIMK2, and Cofilin in the mPFC remain unchanged among all groups (n = 6). All results are expressed as the means ± SEM; * P < .05, ** P < .01; n.s., no significance. The comparisons were made by 2-way ANOVA followed by Bonferroni’s test.

Article Snippet: LIMKi 3 (also named BMS-5, catalog no. T4598) and fluoxetine (catalog no. T0450L) were purchased from Targetmol (Boston, MA).

Techniques: Produced, Expressing

Journal: International Journal of Neuropsychopharmacology

Article Title: LIMK1/2 in the mPFC Plays a Role in Chronic Stress-Induced Depressive-Like Effects in Mice

doi: 10.1093/ijnp/pyaa067

Figure Lengend Snippet: Infusion of LIMKi 3 into the medial prefrontal cortex (mPFC) produced antidepressant-like effects in the chronic restraint stress (CRS) model of depression. (A) Schematic timeline of the experimental procedures. (B) LIMKi 3 significantly attenuated the CRS-induced depressive-like behaviors in mice, as revealed by the forced swim test (FST), tail suspension test (TST), and sucrose preference test (n = 10). (C) Both the (CRS + 100 μM LIMKi 3)-treated and (CRS + 200 μM LIMKi 3)-treated mice had significantly lower levels of pLIMK1, pLIMK2, and pCofilin in the mPFC than the CRS-treated mice (n = 6). The expression of total LIMK1, LIMK2, and Cofilin in the mPFC remained unchanged among all groups (n = 6). All results are expressed as the means ± SEM; ** P < .01; n.s., no significance. The comparisons were made by 2-way ANOVA followed by Bonferroni’s test.

Article Snippet: LIMKi 3 (also named BMS-5, catalog no. T4598) and fluoxetine (catalog no. T0450L) were purchased from Targetmol (Boston, MA).

Techniques: Produced, Expressing

Journal: International Journal of Neuropsychopharmacology

Article Title: LIMK1/2 in the mPFC Plays a Role in Chronic Stress-Induced Depressive-Like Effects in Mice

doi: 10.1093/ijnp/pyaa067

Figure Lengend Snippet: Infusion of LIMKi 3 into the medial prefrontal cortex (mPFC) produced antidepressant-like effects in the chronic social defeat stress (CSDS) model of depression. (A) Schematic timeline of the experimental procedures. (B) LIMKi 3 notably blocked the CSDS-induced depressive-like behaviors in mice, as revealed by the forced swim test (FST), tail suspension test (TST), sucrose preference test, and social interaction test (n = 10). (C) Both the (CSDS + 100 μM LIMKi 3)-treated and (CSDS + 200 μM LIMKi 3)-treated mice had significantly lower levels of pLIMK1, pLIMK2, and pCofilin in the mPFC than those of the CSDS-treated mice (n = 6). The expression of total LIMK1, LIMK2, and Cofilin in the mPFC remained unchanged among all groups (n = 6). All results are expressed as the means ± SEM; ** P < .01; n.s., no significance. The comparisons were made by 2-way ANOVA followed by Bonferroni’s test.

Article Snippet: LIMKi 3 (also named BMS-5, catalog no. T4598) and fluoxetine (catalog no. T0450L) were purchased from Targetmol (Boston, MA).

Techniques: Produced, Expressing